Plant Cell and Tissue Culture

592 Pages·1994·10.448 MB·English

by  Friedrich Constabel, Jerry P. Shyluk (auth.), Indra K. Vasil, Trevor A. Thorpe (eds.)

PLANT CELL AND TISSUE CULTURE
Plant Cell and Tissue Culture
Edited by
INDRA K. VASIL
Laboratory of Plant and Molecular Biology, University of Florida,
Gainesville, FL, U.S.A.
and
TREVOR A. THORPE
Plant Physiology Research Group, Department of Biological Sciences,
The University of Calgary, Alberta, Canada
Springer-Science+Business Media, B.V.
Library of Congress Cataloging-in-Publication Data
ISBN 978-90-481-4327-6 ISBN 978-94-017-2681-8 ( eBook)
DOI 10.1007/978-94-017-2681-8
Reprinted 1996, 1998
03-0298-50 ts
Printed on acid-free paper
All Rights Reserved
© 1994 Springer Science+B usiness Media Dordrecht
Originally published by Kluwer Academic Publishers in 1994
Softcover reprint of the hardcover 1st edition 1994
No part of the material protected by this copyright notice may be reproduced or
utilized in any form or by any means, electronic or mechanical,
including photocopying, recording or by any information storage and
retrieval system, without written permission from the copyright owners.
Table of Contents
Preface vii
List of Contributors IX
PART A: METHODOLOGY
1. Initiation, Nutrition and Maintenance of Plant Cell and Tissue
Cultures
Friedrich Constabel and Jerry P. Shyluk 3
2. Morphogenesis and Regeneration
Trevor A. Thorpe 17
3. Meristem and Shoot Tip Culture: Requirements and
Applications
Narender S. Nehra and Kutty K. Kartha 37
4. Plant Protoplasts for Cell Fusion and Direct DNA Uptake:
Culture and Regeneration Systems
Attila Feher and Denes Dudits 71
5. Isolation and Characterisation of Mutant Cell Lines
Philip J. Dix 119
6. Origins, Causes and Uses of Variation in Plant Tissue Cultures
Angela Karp 139
7. Production and Use of Isogenic Lines
G. Wenzel and B. Foroughi-Wehr 153
8. In vitro Methods for the Control of Fertilization and Embryo
Development
V. Raghavan 173
9. Cryopreservation and Germplasm Storage
K.K. Kartha and F. Engelmann 195
10. Plant Transformation
M.A.W. Hinchee, D.R. Corbin, Ch.L. Armstrong, J.E. Fry,
S.S. Sato, D.L. Deboer, W.L. Petersen, T.A. Armstrong,
D.V. Connor-Ward, J.G. Layton, and R.B. Horsch 231
11. Cell Culture for Production of Secondary Metabolites
F. Constabel and R.T. Tyler 271
v
vi
PART B: IN VITRO CULTURES
12. In vitro Culture of Cereals and Grasses
Indra K. Vasil and Vimla Vasil 293
13. In vitro Culture of Legumes
M.R. Davey, V. Kumar and N. Hammatt 313
14. In vitro Culture of Vegetable Crops
John F. Reynolds 331
15. In vitro Culture of Potato
M.G.K. Jones 363
16. In vitro Culture of Root and Tuber Crops
Abraham D. Krikorian 379
17. In vitro Culture of Oilseeds
C.E. Palmer and W.A. Keller 413
18. In vitro Culture of Temperate Fruits
Richard H. Zimmerman and Harry J. Swartz 457
19. In vitro Culture of Tropical Fruits
J. W. Grosser 475
20. In vitro Culture of Plantation Crops
Abraham D. Krikorian 497
21. In vitro Culture of Forest Trees
Indra S. Harry and Trevor A. Thorpe 539
22. In vitro Culture of Ornamentals
Pierre Debergh 561
Subject Index 575
Species Index 587
Preface
The earliest organized efforts to induce sustained growth of plant cells in
culture were made in the 1930s. They were followed rapidly by the develop
ment of aseptic techniques and complex nutrient media. These advances,
combined with the discovery of plant growth regulators like auxins and
cytokinins, and their profound effects on morphogenesis, led to the regen
eration of plants from cultured tissues. Further refinement of techniques
made it possible to recover whole plants from isolated single cells, through
organogenesis as well as somatic embryogenesis. Today, plant regeneration
can be obtained from cell and tissue cultures of a wide variety of plants,
including most of the economically important species. Indeed, micropropa
gation has become an increasingly important and successful industry in all
parts of the world.
A new phase in the development of plant cell culture techniques started
around 1970 with the regeneration of plants from cultured protoplasts (and
protoplast fusion products), anthers and microspores. At the same time,
newly emerging recombinant DNA technology provided a powerful new tool
for the study of the molecular basis of plant development, as well as their
genetic manipulation and improvement. A wide variety of experimental
procedures were developed for the delivery and stable integration of alien
genes into the germplasm of plants. The synergism of plant cell culture and
molecular biology has led to remarkable advances in our understanding of
plant development and in the production of transgenic plants with valuable
agronomic characteristics. Many such transgenic crops are now undergoing
field trials, and are expected to be commercially available before the end of
this decade.
Eleven chapters in Part A of this volume provide generic accounts of all
of the important aspects of plant cell and tissue culture, from regeneration
to genetic transformation. An equal number of chapters in Part B describe
advances made in the in vitro culture of various economically important
groups of plants. Together, these accounts represent the state of the art of
plant cell and tissue culture.
Each of the chapters in this volume was prepared by distinguished individ
uals who have themselves made pioneering and significant contributions to
their respective fields of study. We are grateful to them for their diligence
vii
viii
in preparing their comprehensive and insightful accounts. It is our hope that
this volume will be useful not only to the newly initiated, but will also be a
source of much valuable information and new ideas for our colleagues inter
ested in the theory as well as the practical utility of plant cell and tissue
cultures.
INDRA K. VASIL
TREVOR A. THORPE
List of Contributors
CoNSTABEL, Friedrich, Plant Biotechnology Institute, National Research Council of Canada, 110
Gymnasium Place, Saskatoon, Saskatchewan, Canada S7N OW9
(Chapter 1 with Jerry P. Shyluk)
(Chapter 11 with R.T. Tyler)
DAVEY, M.R., Plant Genetic Manipulation Group, Department of Life Science, The University
of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
(Chapter 13 with V. Kumar and N. Hammatt)
DEBERGH, Pierre, Laboratory of Horticulture, State University of Ghent, Coupure 653, B-9000
Ghent, Belgium
(Chapter 22)
D1x, Philip J., Department of Biology, St. Patrick's College, Maynooth, County Kildare, Ireland
(Chapter 5)
ENGELMANN, F., ORSTOM, Institut Franr;ais de Recherche Scientifique pour le Developpement
et Cooperation, Montpellier, France
(Co-author of Chapter 9)
FEHER, Attila, Institute of Plant Biology, Biological Research Centre, Hungarian Academy of
Sciences, Temesvdri Krt 62, P.O. Box 521, H-6701 Szeged, Hungary
(Chapter 4 with Denes Dudits)
GROSSER, J. W., IFAS, Citrus Research and Education Center, University of Florida, Lake Alfred,
FL 33850, USA
(Chapter 19)
HAMMATT, N., Horticulture Research International, East Malting, Kent ME19 6BJ, United
Kingdom
(Co-author of Chapter 13)
HARRY, Indra S., Plant Physiology Research Group, Department of Biological Sciences, The
University of Calgary, 2500 University Drive NW, Calgary, Alberta, Canada T2N 1N4
(Chapter 21 with Trevor A. Thorpe)
HINcliEE, Maud A. W., Monsanto Plant Protection Improvement Institute, 700 Chesterfield VP,
St. Louis, MO 63198, USA
(Chapter 10 with David R. Corbin, Charles L. Armstrong, Joyce E. Fry, Shirley S. Sato,
David L. Deboer, William L. Petersen, Toni A. Armstrong, Danette V. Connor-Ward,
Jeanne G. Layton, and Robert B. Horsch)
JoNES, M.G.K., Plant Sciences, School of Biological and Environmental Science, Murdoch
University, Perth, WA 6150, Australia
(Chapter 15)
KARP, Angela, Department of Agricultural Sciences, University of Bristol, AFRC Institute of
Arable Crops Research, Long Ashton Research Station, Bristol, BS18 9AF, United Kingdom
(Chapter 6)
ix
X
KARTHA, Kutty K., Plant Biotechnology Institute, National Research Council of Canada, 110
Gymnasium Place, Saskatoon, Saskatchewan, Canada S7N OW9
(Co-author of Chapter 3)
(Chapter 9 with F. Engelmann)
KELLER, W.A., Plant Biotechnology Institute, National Research Council of Canada, 110
Gymnasium Place, Saskatoon, Saskatchewan, Canada S7N OW9
(Co-author of Chapter 17)
KRIKORIAN, Abraham D., Department of Biochemistry and Cell Biology, State University of
New York at Stony Brook, Stony Brook, NY 11794-5215, USA
(Chapters 16 and 20)
KuMAR, V., Plant Genetic Manipulation Group, Department of Life Science, University of
Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
(Co-author of Chapter 13)
NEHRA, Narender S., Plant Biotechnology Institute, National Research Council of Canada, 110
Gymnasium Place, Saskatoon, Saskatchewan, Canada S7N OW9
(Chapter 3 with Kutty K. Kartha)
PALMER, C.E., Department of Plant Science, University of Manitoba, Winnipeg, Manitoba,
Canada R3T 2N2
(Chapter 17 with W.A. Keller)
RAGHAVAN, V., Department of Plant Biology, The Ohio State University, 1735 Neil Avenue,
Columbus, OH 43210-1293, USA
(Chapter 8)
REYNOLDS, John F., Experimental Plant Genetics, The Up john Company, Kalamazoo, MI 49002,
USA
(Chapter 14)
SwARTZ, Harry S., Department of Horticulture, University of Maryland, College Park, MD
20742, USA
(Co-author of Chapter 18)
THORPE, Trevor A., Plant Physiology Research Group, Department of Biological Sciences, The
University of Calgary, 2500 University Drive NW, Calgary, Alberta, Canada T2N 1N4
(Chapter 2)
(Co-author of Chapter 21)
TYLER, R.T., Department of Applied Microbiology and Food Science, University of Sas
katchewan, Saskatoon, Saskatchewan, Canada S7N OWO
(Co-author of Chapter 11)
VASIL, Indra K., Laboratory of Plant Cell and Molecular Biology, Department of Horticultural
Sciences, 1143 Fifield Hall, University of Florida, Cainsville, FL 32611-0690, USA
(Chapter 12 with Vimla Vasil)
WENZEL, G., Federal Biological Research Centre for Agriculture and Forestry, Institute of Resis
tance Genetics, W-8059 Grilnbach, Germany
(Chapter 7 with B. Foroughi-Wehr)
ZIMMERMAN, Richard H., US Department of Agriculture, Agricultural Research Service
(USDA/ARS), Plant Sciences Institute, Fruit Laboratory, Bldg 004, Room 120, 10300 Balti
more Avenue, Beltsville, MD 20705-2350, USA
(Chapter 18 with Harry J. Swartz)
PART A
Methodology