ebook img

Plant Biotechnology and Medicinal Plants: Periwinkle, Milk Thistle and Foxglove PDF

213 Pages·2019·3.14 MB·English
Save to my drive
Quick download
Download
Most books are stored in the elastic cloud where traffic is expensive. For this reason, we have a limit on daily download.

Preview Plant Biotechnology and Medicinal Plants: Periwinkle, Milk Thistle and Foxglove

Mohamed Ramadan Rady Plant Biotechnology and Medicinal Plants Periwinkle, Milk Thistle and Foxglove Plant Biotechnology and Medicinal Plants Mohamed Ramadan Rady Plant Biotechnology and Medicinal Plants Periwinkle, Milk Thistle and Foxglove Mohamed Ramadan Rady Department of Plant Biotechnology National Research Centre Giza, Egypt ISBN 978-3-030-22928-3 ISBN 978-3-030-22929-0 (eBook) https://doi.org/10.1007/978-3-030-22929-0 © Springer Nature Switzerland AG 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Springer imprint is published by the registered company Springer Nature Switzerland AG The registered company address is: Gewerbestrasse 11, 6330 Cham, Switzerland Dedicated to my beloved family, Ayah, Achraf, and Salwa Preface Recently, secondary metabolite production through plant cell culture and plant biotechnology has attracted interest from scientists. The potential of some plant culture systems for the production of medically important compounds has been demonstrated. Recently, genetic transformation and metabolic engineering are important areas which may provide new ways and efficient systems to increase in vitro production of secondary metabolites in medicinal plants. This system can now provide a commercially realistic alternative to whole plants for the production of some drugs. More recently, the progress on the genetic manipulation of biosyn- thetic units in microorganisms (synthetic biology) has opened the possibility of in-depth exploration of the large chemical space of natural product derivatives. In spite of several successful reports on the studied plants, there is still a gap in the knowledge for biosynthetic pathways and upscaling of this culture system for commercial utilization. The purpose of this book is to provide recent information and studies about the induction of cell and organ cultures, establishment of transgenic cultures, and induction of hairy roots from in vitro cultures of periwinkle, milk thistle, and fox- glove plants with special emphasis on elicitation strategies by abiotic and biotic elicitors for the production of the bioactive compounds from cultures. This book is expected to serve as a guide for the use of plant biotechnology as alternative source for increasing pharmaceutically important anticancer, flavonolignan, and cardeno- lide compounds from the studied plants. This book will be valuable to researchers as well as students working in the area of medicinal plant biotechnology. It will also serve as a reference for the pharmaceutical industry. Giza, Egypt Mohamed Ramadan Rady vii Acknowledgment Foremost, I would like to thank Allah, without whom, nothing is possible. I dedicate this work to my deceased parents who gave me the best gift of all, higher education and the freedom of choice. Also, I owe a special thanks to my brothers for their love and unwavering support throughout my life and to Dr. Kenneth Teng, the editorial staff of Springer, New York, for his guidance and encouragement and for giving me the opportunity to conduct this book, without him, there would be no book at all. I would also like to acknowledge the Springer team for their help in technical and continuous support and for editing this book. Last, but not least, I would like to gratefully acknowledge the National Research Centre, Egypt, for their constant sup- port and excellent care. Mohamed Ramadan Rady ix Contents 1 Plant Biotechnology and Periwinkle . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 2 Biosynthesis of TIA in C. roseus . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3 In Vitro Culture of C. roseus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 3.1 Cell and Callus Cultures of C. roseus . . . . . . . . . . . . . . . . . 7 3.2 Plant Regeneration of C. roseus . . . . . . . . . . . . . . . . . . . . . . 14 3.3 Cryopreservation of C. roseus . . . . . . . . . . . . . . . . . . . . . . . 24 3.4 Genetic Transformation of C. roseus . . . . . . . . . . . . . . . . . . 29 4 Terpenoid Indole Alkaloid Production from In Vitro Culture of Catharanthus roseus Through Biotic, Abiotic Elicitation and Precursor Feeding . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 4.1 Abiotic Elicitation and TIA Production from In Vitro Cultures of C. roseus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 4.2 Biotic Elicitation and TIA Production from In Vitro Cultures of C. roseus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 4.3 Precursor Feeding and TIA Production from In Vitro Cultures of C. roseus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 5 C onclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 6 F uture Aspects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87 2 P lant Biotechnology and Milk Thistle . . . . . . . . . . . . . . . . . . . . . . . . . . 97 1 I ntroduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98 2 B iosynthesis of Silymarin in S. marianum . . . . . . . . . . . . . . . . . . . 99 3 In Vitro Culture of S. marianum . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 3.1 Cell and Callus Cultures of S. marianum. . . . . . . . . . . . . . . 101 3.2 Plant Regeneration of S. marianum . . . . . . . . . . . . . . . . . . . 106 3.3 Root Cultures of S. marianum . . . . . . . . . . . . . . . . . . . . . . . 109 3.4 Genetic Transformation of S. marianum . . . . . . . . . . . . . . . 111 xi xii Contents 4 Silymarin Production from In Vitro Culture of S. marianum Through Biotic and Abiotic Elicitation and Precursor Feeding . . . . 114 4.1 Abiotic Elicitation and Silymarin Production from In Vitro Cultures of Silybum marianum . . . . . . . . . . . 114 4.2 Biotic Elicitation and Silymarin Production from In Vitro Culture of Silybum marianum . . . . . . . . . . . . 133 4.3 Precursor Feeding and Silymarin Production from In Vitro Culture of Silybum marianum . . . . . . . . . . . . 138 5 Conclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140 6 Future Aspects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142 3 Plant Biotechnology and Foxglove . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150 2 Biosynthesis of Cardenolides in Digitalis ssp. . . . . . . . . . . . . . . . . 151 3 In Vitro Culture of Digitalis ssp. . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 3.1 C ell and Callus Cultures of Digitalis ssp. . . . . . . . . . . . . . . 153 3.2 Plant Regeneration of Digitalis ssp. . . . . . . . . . . . . . . . . . . . 155 3.3 Cryopreservation of Digitalis ssp. . . . . . . . . . . . . . . . . . . . . 164 3.4 Genetic Transformation of Digitalis ssp. . . . . . . . . . . . . . . . 167 4 C ardenolide Production from In Vitro Culture of Digitalis ssp. Through Biotic, Abiotic Elicitation and Precursor Feeding . . . . . . . 171 4.1 A biotic Elicitation and Cardenolide Production from In Vitro Cultures of Digitalis ssp. . . . . . . . . . . . . . . . . 171 4.2 B iotic Elicitation and Cardenolide Production from In Vitro Cultures of Digitalis ssp. . . . . . . . . . . . . . . . . 182 4.3 P recursor Feeding and Cardenolide Production from In Vitro Cultures of Digitalis ssp. . . . . . . . . . . . . . . . . 185 5 A ccumulation of Cardenolides in In Vitro Culture of Digitalis spp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 186 6 Conclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 192 7 Future Aspects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 193 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 194 Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 199 Abbreviations ABT 1-aminobenzotriazole ACES 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid AgNO Silver nitrate 3 APX1 Ascorbate peroxidase 1 AS Anthranilate synthase AVG Aminoethoxyvinylglycine B5 Gamborg et al. (1968) medium BA Benzyl adenine CA Coniferyl alcohol CaCI Calcium chloride 2 CAD Cinnamyl alcohol dehydrogenase Cd(NO) Cadmium nitrate 3 2 CDPK Calcium-dependent protein kinases CDs Cyclodextrins CH Casein hydrolase CHI Chalcone isomerase CHS Chalcone synthase chsA Chalcone synthase gene CLOT Clotrimazole CPA Chlorophenoxyacetic acid CuSO Copper sulfate 4 D4H Deacetoxyvindoline 4-hydroxylase DMAPP Dimethylallyl diphosphate (DMAPP) DMSO Dimethyl sulfoxide EGTA Ethylene glycol tetraacetic acid ER Endoplasmic reticulum FeSO Iron sulfate 4 G(G)PPS Geranyl (geranyl) diphosphate synthase G10H Geraniol 10-hydroxylase GA Gibberellic acid 3 GES Geraniol synthase xiii

See more

The list of books you might like

Most books are stored in the elastic cloud where traffic is expensive. For this reason, we have a limit on daily download.