Plant Abiotic Stress-

146 Pages·2009·1.43 MB·English

Plant Abiotic Stress-
from signaling to development
2nd meeting of the INPAS*
14-17 May 2009 Tartu Estonia
*International Network of Plant Abiotic Stress (INPAS)
is supported by EU COST action FA0605
Plant Abiotic Stress –
from signaling to development
Tartu, Estonia
14-17 May 2009
Scientific Committee:
Antonio F. Tiburcio (Universtat de Barcelona, Spain, chair of INPAS, cost-inpas.org)
Dorothea Bartels (University of Bonn, Germany)
Laszlo Bogre (Royal Holloway University of London, UK)
Pedro Carrasco (Universitat de Valencia, Spain)
Rina Iannacone (Metapontum Agrobios S.S., Italy)
Hannes Kollist (University of Tartu, Estonia)
Csaba Koncz (Max-Planck Institute, Köln, Germany)
Ülo Niinemets (Estonian University of Life Sciences, Estonia)
Ioanna Stavridou (COST FA0605 Science Officer)
Laszlo Szabados (Biological Research Center, Hungary)
Bernd Wollenweber (Aarhus University)
Aviah Zilberstein (Tel Aviv University, Israel)
Local organizing Committee:
Hannes Kollist
Krisiina Laanemets
Ove Lindgren
Liina Margna
Ebe Merilo
Heino Moldau
Priit Pechter
Irina Puzõrjova
Triin Vahisalu
Ülo Niinemets
Steffen M. Noe
Cover photo by Ülle Kollist
Design of conference materials by Tanel Vahisalu
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Welcome
Plants, unlike animals, cannot move. This requires that adverse changes in their environment are rapidly
recognized, discerned and responded to with adequate reactions. Cold, salinity and drought are among the
major stresses that adversely affect plant growth and productivity. In fact, these abiotic stresses represent
the main cause of crop failure worldwide, dipping average yields for major crops by more than 50%. Since
the world population is increasing at an alarming rate, minimizing these losses is also a major concern for all
nations coping with the increasing food demand.
The overall aim of the conference is to provide a platform for interdisciplinary discussions between scientists
dealing with different aspects of plants abiotic stress – from signaling to development. We hope that the
efforts of the speakers will be rewarded by active discussions during the sessions and throughout the whole
conference.
The meeting is organized and supported by the European Cooperation in Science and Technology (COST)
Action FA0605 entitled “Signalling control of stress tolerance and production of stress protective compounds
in plants” (2007-2011). This is an International Network of Plant Abiotic Stress (INPAS, cost-inpas.org) that
stimulates collaborations between complementary activities of experts working in various fields of stress
biology and is currently composed by 58 partners from 28 participating countries.
We also thank the support from the Estonian Ministry of Education and Research and from our sponsors.
Finally, it is our great pleasure to welcome you to this conference. We hope that these few days in Tartu will
include professional highlights and social events to be remembered for all participants.
Antonio F. Tiburcio (chair of INPAS)
Hannes Kollist (chair of local organizing committee)
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Table of Contents
Programme 4
Abstracts 6
Oral Presentations 7
Poster Presentations 39
List of Authors 128
Maps and travel Information 137
Sponsors 139
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Programme
DAY 1 (14th of May, Thursday)
17:00-22:00 Registration and poster set up
20:30-22:00 Welcome refreshments
DAY 2 (15th of May, Friday)
8:00-9:00 Registration and poster set up
9:00-9:15 Welcome note by Antonio Tiburcio, chair of INPAS
ABIOTIC STRESS SIGNALLING
Chair: Simon Gilroy
9:15-9:45 Ülo Niinemets (Estonia): Scaling abiotic stress resistance from molecular mechanisms to
the field
9:45-10:10 Tamas Dalmay (UK): Plant short RNAs and stress
10:10-10:30 Irute Meskiene (Austria): Stress and Plant Developmental control by protein phosphatases
10:30-10:50 Tony Remans (Netherlands): Elucidating the molecular triggers of root developmental
responses to heavy metal stress
10:50-11:10 Teun Munnik (Netherlands): Phospholipid Signaling in Plant Stress & Development
11:10-11:40 Coffee break
Chair: Dorothea Bartels
11:40-12:10 Montserrat Pages (Spain): Drought tolerance in maize, an important crop in agriculture
12:10-12:30 Laszlo Bogre (UK): Signalling pathways regulating the extent and directionality of plant
growth in response to environmental stress factors and during development
12:30-12:50 Dudy Bar-Zvi (Israel): Structure function of tomato ASR1 - a plant specific stress regulated
hydrophilin
12:50-13:10 Natalia Stepanchenko (Russia): Cross-talk between ethylene and abscisic acid signaling
pathways mediates proliferation of Arabidopsis thaliana cultivated cells
13:10-14:30 Lunch
GENETICS AND NATURAL VARIATION
Chair: Laszlo Szabados
14:30-15:00 Matthieu Reymond (Germany): Genetic and molecular basis of plant performance using
natural variation in Arabidopsis thaliana
15:00-15:20 Arnd Heyer (Germany): Mathematical Modelling of Acclimation to low Temperature Re-
veals Contrasting Strategies in Natural Accessions of Arabidopsis thaliana
15:20-15:40 Ruben Alcazar (Germany): Environmental dependence of genetic epistatic networks
modulating growth, immune responses and speciation processes in Arabidopsis
15:40-16:00 Gad Galili (Israel): Principal transcriptional programs regulating plant metabolism in re-
sponse to abiotic stresses
16:00-16:30 Coffee break
Chair: Ülo Niinemets
16:30-17:00 John Doonan (UK): Natural variation in cell growth: roles in adaptation to environmental
stresses
17:00-17:20 Arnould Savoure (France): Opposite stress signalling pathways are present in Arabidopsis
thaliana and in Thellungiella halophila
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17:20-17:40 Myriam Olortegui (Netherlands): Natural genetic variation of abiotic multi-stress responses
in Arabidopsis
17:40-18:00 Mary Prathiba (Hungary): A Novel genetic approach for Identifying genes involved in absci-
sic acid regulation
18:00-19:30 Free time
19:30-22:00 Poster session with beer/wine/buffet
DAY 3 (16th of May, Saturday)
PHYSIOLOGY, BIOCHEMISTRY & METABOLIC PROFILES
Chair: Hannes Kollist
9:00-9:30 Rainer Hedrich (Germany): Guard cells in action
9:30-10:00 Maria Israelsson Nordström (Sweden): Keeping up with changing CO levels- new
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insights from the guard cells
10:00-10:20 Irina Puzõrjova (Estonia): Over-expression of ERD15 affects stomatal response to several
atmospheric stimuli
10:20-10:40 Triin Vahisalu (Estonia): Key proteins in governing stomatal response to ozone and induc-
tion of reactive oxygen species in plants
10:40-11:00 Kumud Mishra (Czech Republic): Feasibility experiments for developing tools and method-
ology for non-invasive sensing of drought resistance in tomato transgenics
11:00-11:30 Coffee break
Chair: Antonio Tiburcio
11:30-12:00 Alain Bouchereau (France): A comparative functional analysis of salt and osmotic stress
metabolomes in Thellungiella halophila and Arabidopsis thaliana
12:00-12:20 Teresa Altabella (Spain): Putrescine as signaling molecule involved in the control of stress
responses to cold and drought
12:20-12:40 Claudia Jonak (Austria): High soil salinity: Metabolic adaptation, redox balance and signal-
ling
12:40-13:00 Aviah Zilberstein (Israel): Newly Identified Cytosolic-Mitochondrial Proline-P5C Cycle in
Plants
13:00-14:30 Lunch
ABIOTIC STRESS AND DEVELOPMENT
Chair: Pedro Carrasco
14:30-15:00 Simon Gilroy (USA): Feeling green: mechanotransduction in Arabidopsis growth and
development
15:00-15:20 Seth Davis (Germany): Redox stress is a major component of circadian-clock resetting in
response to dawn
15:20-15:40 Carlos S. Galvan-Ampudia (Netherlands): Osmotic stress-induced signals control root
growth
15:40-16:00 Margarete Müller (Germany): UBP14 is involved in root hair development under phosphate
starvation in Arabidopsis
16:00-16:30 Coffee Break
16:30-18:00 Management Committee Meeting
18:00-20:00 Sightseeing Tour
20:00-03:00 Closing Dinner in Gunpowder Cellar
DAY 4 (17th of May, Sunday)
6:00-12:00 Dismounting posters
6:00-12:00 Checkout from Dorpat hotel
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Abstracts
6
Oral Presentations
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SCALING ABIOTIC STRESS RESISTANCE FROM MOLECULAR MECHANISMS TO THE FIELD
Ülo NIINEMETS
Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi 1,
51014 Tartu, Estonia
Global climate change implies simultaneous modification of multiple environmental drivers (CO , water availa-
2
bility and temperature), but the mechanisms of acclimation to interacting environmental stresses are still poor-
ly understood. To understand such responses to interactive environmental variables, structural and physio-
logical controls on photosynthetic and respiratory acclimation and stress-induced volatile organic compound
(volatile plant hormones and volatile compounds produced during oxidative signalling) emissions are studied.
Lab results in various model plants with varying longevity and abiotic stress resistance (wild and mutant geno-
types of Arabidopsis, Nicotiana and Populus) are transferred to natural environments by field experiments and
mathematical modelling using scenario analyses to predict plant performance in future climates. The presen-
tation emphasizes the need to study plant abiotic stress resistance in model systems with various levels of
constitutive stress tolerance and potential to acclimate to altered environments.
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CENTRAL ROLES OF THE SNRK2 SUBFAMILY OF PROTEIN KINASES IN ABA SIGNALING
Jian-Kang ZHU, Hiroaki FUJII
Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA,
[email protected]
Abscisic acid (ABA) is an important phytohormone regulating seed dormancy, germination, seedling growth,
and plant transpiration. We report here an Arabidopsis triple mutant that is disrupted in three SnRK2s (SNF1-
Related Protein Kinase Subfamily 2) and nearly completely insensitive to ABA. These SnRK2s, SnRK2.2,
SnRK2.3 and SnRK2.6 (also known as OST1), are activated by ABA and can phosphorylate the ABF (ABA-
Responsive Element Binding Factor) family of b-ZIP transcription factors, which are important for the activation
of ABA-responsive genes. Though stomatal regulation of snrk2.6 and seed germination and seedling growth
of the snrk2.2/2.3 double mutant are insensitive to ABA, ABA responses are still present in these mutants, and
the growth and reproduction of these mutants are not very different from those of the wild type. In contrast,
the snrk2.2/2.3/2.6 triple mutant grows poorly and produces few seeds. The triple mutant plants lose water
extremely fast when ambient humidity is not high. Even on 50 µM ABA, the triple mutant can germinate and
grow, whereas the most insensitive known mutants cannot develop on 10 µM ABA. In-gel kinase assays showed
that all ABA-activated protein kinase activities are eliminated in the triple mutant. Furthermore, the expression
of ABA-induced genes examined is completely blocked in the triple mutant. These results demonstrate that the
protein kinases SnRK2.2, 2.3, and 2.6 have redundant functions, and suggest that ABA signaling is critical for
plant growth and reproduction.
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