Physiological, Anatomical and Gene Expression studies under Drought among F1 Progenies of Musa

120 Pages·2012·16.54 MB·English

Physiological, Anatomical and
Gene Expression studies under
Drought among F Progenies of Musa
1

MADHURI.P
PAL 0227

DEPARTMENT OF PLANT BIOTECHNOLOGY
UNIVERSITY OF AGRICULTURAL SCIENCES
BENGALURU
2012
Physiological, Anatomical and Gene
Expression studies under Drought
among F Progenies of Musa
1

MADHURI.P
PAL 0227

Thesis submitted to the
University of Agricultural Sciences, Bengaluru
in partial fulfillment of the requirements
for the award of the Degree of
Master of Science (Agriculture)
in
Plant Biotechnology

BENGALURU JULY, 2012
Affectionately
Dedicated to
My beloved

Parents
DEPARTMENT OF PLANT BIOTECHNOLOGY
UNIVERSITY OF AGRICULTURAL SCIENCES
BENGALURU-560065
CERTIFICATE
This is to certify that the thesis entitled “Physiological, Anatomical
and Gene expression studies under drought among F
1
progenies of Musa” submitted by Ms. MADHURI.P, ID. No. PAL
0227 in partial fulfilment of the requirement for the degree of Master of
Science (Agriculture) in Plant Biotechnology to the University of
Agricultural Sciences, Bengaluru, is a record of bonafide research work
done by her during the period of her study in this University under my
guidance and supervision and that no part of the thesis has previously
formed the basis for the award of any degree, diploma, associate ship,
fellowship or other similar titles.
Bangalore
July, 2012 Dr. K.V. RAVISHANKAR
Senior scientist
Division of Biotechnology
IIHR, Hessaraghatta, Bangalore
(MAJOR ADVISOR)
Approved by:
Chairman : ___________________________
(K.V. RAVISHANKAR)
Members : 1. ___________________________
(P.H. RAMANJINI GOWDA)
2. ___________________________
(NATARAJA KARABA)
3. ___________________________
(LAXMAN, R.H.)
4. ___________________________
(REKHA, A.)
5. ___________________________
(EARANNA, N.)
Acknowledgement
The task of acknowledging the help that was offered to me throughout this
study by my teachers and friends is bigger than the study itself. I feel scanty of words
to the magnitude of their help. I could not have completed this work without enjoying
their endless patience and affection. Under this decorum I would like to recall all of
them with utmost gratitude.
Would like to convey my sincere gratitude and thanks to all those who have
been instrumental in the course of my studies at the University of Agricultural
Sciences, Bengaluru. It is not possible to mention the names of all the individuals
who contributed to this piece of work but I fully recognize and appreciate your
valuable contributions.
I am very grateful to Department of Biotechnology which made possible for
me to undertake these studies and accomplish this research work.
My deep gratitude goes to my chair person Dr. K. V. RAVISHANKAR for his
valuable guidance and encouragement during my entire postgraduate study. Mere
any words from the vastness of literature fails to express my sense of gratitude to him,
for his continued calm endurance shown throughout my research programme and
compilation of this thesis, without which this work would not have seen the light of the
day.
It gives me great pleasure to express my whole hearted thanks and reverential
regards to my Advisory committee members Dr. Rekha.A, Senior Scientist, Division
of Fruit crops, IIHR, Hessaraghatta, Bangalore, Dr. P.H. Ramanjini Gowda,
professor, Department of plant Biotechnology ,GKVK, Bangalore, Dr. Laxman R.H,
senior scientist Division of plant physiology IIHR, Hessaraghatta, Bangalore,
Dr. Nataraja Karaba, Associate professor, Department of crop physiology, GKVK
Bangalore, Dr. Earanna associate professor, Department of plant Biotechnology
,GKVK, Bangalore, for their inspiring guidance at every step, continuous
encouragement, motivational support, constant supervision and discreet suggestions
which helped me to complete research successfully.
I am extremely thankful to Dr. T.H. Ashok, Professor and Head, Department
Biotechnology and all the staff members Dr. Dayal Doss, Dr. C.K. Suresh,
Dr. Shyamalamma, Dr. Anitha Peter, Dr. Theertha Prasad, and Dr. K.M.
Harinikumar who have provided an excellent studying atmosphere during my
Masters programme.
I am over whelmed with sincere feelings of indebtedness to my beloved
parents, Mrs. Manjula prakash & Mr. R.C. Prakash Kumar sister Smt. Vasundhara
Satish My Brother in law Mr. Satish K.N and their lovely son Master Harith Suman
I could not been able to attain the present stage without their blessings, love and
support.
Special thanks are also extended to all my friends and lab mates Mr. John
Sunoj, Ms. Puneeta, Ms. Vinenthitha, Ms. Akhila Rao, Ms.Pavitra, Param sir, for
their help.
I also thank all my classmates and friends for their support and help they
have extended in whole degree program.
I have absolutely no words to express my sincere thanks to labours of our
field, who helped me immensely throughout the course of investigation in the field
such as Julakshi, sreenivas, Krishna, Hanumantha raju, Muniraju
The non-teaching staff members of Dept. of Biotechnology were every bit
understanding. They were kind and provided all the support they could.
……………..Any omission in this brief acknowledgement does not mean lack of
gratitude.
Bengaluru,
July, 2012 MADHURI. P
PHYSIOLOGICAL, ANATOMICAL AND GENE EXPRESSION STUDIES
UNDER DROUGHT AMONG F PROGENIES OF MUSA
1

MADHURI.P

ABSTRACT
The present study examines the physiological and molecular
behaviour of some of the F progenies obtained from the cross between
1
M.acuminata. spp burmaniceoides commonly recognised as ‘Calcutta-4’
(AA), having high stomatal density (388 total stomata/cm2), and ‘Bee Hee
Kela’ (BB), having low stomatal density (313 total stomata/cm2). Two
each F progenies from group having high (D-22, D-40), medium (D-37,
1
D-19) and low stomatal density (D-5, D-51) were physiologically
evaluated for photosynthesis rate (PN), stomatal conductance (gs) and
transpiration rate (E) and some of the root morphological characters.
Among the parents ‘Bee hee kela’ (BB) showed high PN, E, gs,
specific leaf weight, leaf water retention capacity, root length, root
volume and leaf epicuticular wax content than Calcutta-4(AA). Medium
stomata density group (D-19, D-37) showed higher stomatal conductance
(0.18 mol H O. m-2. sec-1) than the parents (0.13 mol H O. m-2. sec-1),
2 2
and F ’s with high stomatal density (D-22 and D-40) showed higher
1
water use efficiency (WUE;5.9 gm.dm/L).
Stomatal conductance in parents and progenies was positively
correlated with net photosynthesis (r=0.91) and there was no significant
correlation between stomatal density and photosynthesis, transpiration
rate. cDNA was amplified with primers designed for selected candidate
stomatal development genes namely YODA, ERECTA and FLP.

Signature of the Student Signature of the Major Advisor
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F
1
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1
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¸Àå
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ªÀÄÆ® ¸À UÀ½VAvÀ ºÉZÀÄÑ ¸ÉÆÖªÀiÁål ªÁºÀPÀvÉAiÀÄ£ÀÄß( ) vÉÆÃj¹zÀªÀÅ. r-
¸Àå 0.13 mol H2O. m-2. sec-1 22
ºÁUÀÆ r- ¸À¹UÀ¼ÀÄ G½zÉgÉqÀÄ UÀÄA¦VAvÀ®Æ ºÉZÀÄÑ ¤ÃgÀÄ §¼ÀPÉ ¸ÁªÀÄxÀåð( )ªÀ£ÀÄß
40 WUE
vÉÆÃj¹zÉ(5.9 gm.dm/L).
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F
1
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r=0.91
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.
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WUE
ºÁUÀÆ F ªÀA±À¸ÀÜ ¸À¹UÀ¼À ¹- rJ£ïJAiÀÄ£ÀÄß ¸ÉÆÖªÀiÁåmÁ ¨É¼ÀªÀtÂUÉUÉ CUÀvÀåªÁzÀ PÉ®ªÀÅ
1
ªÀA±ÀªÁ»UÀ¼ÁzÀ YODA, ERECTA ªÀÄvÀÄÛ FLP UÀ¼À£ÀÄß ¥ÉæöʪÀÄgïUÀ¼À ¸ÀºÁAiÀÄ¢AzÀ C©üªÀåQÛ
CzsÀåAiÀÄ£ÀPÉÌ M¼À¥Àr¸À¯Á¬ÄvÀÄ.

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CONTENTS
CHAPTER TITLE Page No.
I INTRODUCTION 1 - 5
II REVIEW OF LITERATURE 6 - 26
III MATERIAL AND METHODS 27 - 42
IV RESULTS 43 - 55
V DISCUSSION 56 - 61
VI SUMMARY 62 - 64
VII REFERENCES 65 - 86
VIII ANNEXURE
LIST OF TABLES
Table Page
Title
No. No.
1. List of primers used for molecular analysis 38
2. Stomatal density studies of parents and F progenies 44
1
F plants selected for further physiological and
1
3. 45
molecular studies.
Gas exchange parameters recorded among different
4. 48
Banana genotypes differing in stomatal density.
Differences in physiological and biochemical
5. characteristics among different Banana genotypes 49
differing in stomatal density.
Correlation co efficient values and level of significance
6. 50
among correlations between Physiological parameters.
Root characters among different Banana genotypes
7. 51
differing in stomatal density.
Quantification of RNA samples isolated from well
8. watered and stressed leaf samples of selected Banana 53
genotypes.
Expression of different stomatal genes in selected
9. 55
genotypes.