Photoactivated antiviral and antitumor compositions

27 Pages·2016·1.73 MB·English

Iowa State University Patents Iowa State University Research Foundation, Inc.
7-14-1998
Photoactivated antiviral and antitumor
compositions
George A. Kraus
Iowa State University, [email protected]
Susan L. Carpenter
Iowa State University, [email protected]
Jacob W. Petrich
Iowa State University, [email protected]
Follow this and additional works at:http://lib.dr.iastate.edu/patents
Part of theAnimal Sciences Commons, and theChemistry Commons
Recommended Citation
Kraus, George A.; Carpenter, Susan L.; and Petrich, Jacob W., "Photoactivated antiviral and antitumor compositions" (1998).Iowa
State University Patents. 215.
http://lib.dr.iastate.edu/patents/215
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Photoactivated antiviral and antitumor compositions
Abstract
Disclosed herein are compounds, compositions, and methods to inactivate a virus and destroy tumor cells.
The methods involve the addition into the cell of a compound containing a photosensitizing chemical and an
energy donating chemical, optionally linked by a chemical tether. Also introduced into the cell are means to
chemically activate the energy donating chemical which photoactivates the photosensitizing chemical which
then destroys the tumor or virus. The photosensitizing chemical is preferably hypericin, porphyrin, or an
analog and the energy donating chemical is preferably luciferin or an analog. Methods for synthesizing the
chemicals are also disclosed. Further, the energy donating chemical is activated by an activating chemical. The
expression of the activating chemical is regulated so as to target the virus-infected or tumor cells. Regulating
the activating chemical is accomplished by a number of methods including construction of an expression
plasmid containing a gene encoding the activating chemical under control of a promoter which is
transactivated by replication of the virus or transactivated by elevated levels of proteins expressed in tumor
cells.
Keywords
Chemistry, Animal Science
Disciplines
Animal Sciences | Chemistry
This patent is available at Iowa State University Digital Repository:http://lib.dr.iastate.edu/patents/215
USOU5780287A
United States Patent [19]
[11] Patent Number: 5,780,287
Kraus et al. [45] Date of Patent: Jul. 14, 1998
[54] PHOTOACTIVATED ANT [VIRAL AND Meruelo et al.. “Therapeutic agents with dramatic antiret
ANTITUMOR COMPOSITIONS roviral activity and little toxicity at effective doses: Aromatic
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[51] rm. c1.6 ............................. .. C12N 7/04; C12N 7/06:
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#I‘M0O3. Oct. 1991.
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[57] ABSTRACT
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Rodriguez. et a1. 1988. PNAS. USA vol. 85 pp. 1667-1671. Disclosed herein are compounds. compositions. and meth
Lenard. et al.. “Photodynamic inactivation of infectivity of ods to inactivate a virus and destroy tumor cells. The
human immunodeficiency virus and other enveloped viruses methods involve the addition into the cell of a compound
using hypericin and rose bengal: Inhibition of fusion and containing a photosensitizing chemical and an energy donat
syncytia formation” Proc. Natl. Acad. Sci, USA. 90: 158-162 ing chemical. optionally linked by a chemical tether. Also
(Jan. 1993)). introduced into the cell are means to chemically activate the
Van Steveninck et al.. “Photodynamic Effects Induced by the energy donating chemical which photoactivates the photo
Luciferin/Luciferase System.” Photochemistry and Photo sensitizing chemical which then destroys the tumor or virus.
biology. 43:2. 213-216 (1986). The photosensitizing chemical is preferably hypericin.
Carpenter et al.. “Identi?cation of a Hypervan'able Region in porphyrin. or an analog and the energy donating chemical is
the Long Terminal Repeat of Equine Infectious Anemia preferably luciferin or an analog. Methods for synthesizing
Virus.” Journal of Virology. 65:3. 1605-1610 (Mar. 1991). the chemicals are also disclosed. Further. the energy donat
ing chemical is activated by an activating chemical. The
Kraus et al.. “Antiretroviral Activity of Synthetic Hypericin
expression of the activating chemical is regulated so as to
and Related Analogs.” Biochemical and Biophysical
target the virus-infected or tumor cells. Regulating the
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activating chemical is accomplished by a number of meth
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ods including construction of an expression plasmid con
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control of a promoter which is transactivated by replication
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125:4-6 (Apr. 19. 1991). expressed in tumor cells.
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14621-4 (Mar. 6. 1992). 15 Claims, 6 Drawing Sheets
5,780,287
Page 2
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Sci, USA. vol. 87. pp. 473-477. Jan. 1990. Therapy. Retroviruses and Disease. 1989.
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