Pharmacognosy-2014 Accepted Abstracts

106 Pages·2014·1.52 MB·English

199th OMICS Group Conference
2nd International Conference and Exhibition on
Pharmacognosy,
Phytochemistry & Natural Products
August 25-27, 2014 DoubleTree by Hilton Beijing, China
Accepted Abstracts
Pharmacognosy-2014
Page 131
Samreen Saleem et al., Nat Prod Chem Res 2014, 2:5
http://dx.doi.org/10.4172/2329-6836.S1.005
2nd International Conference and Exhibition on
Pharmacognosy, Phytochemistry & Natural Products
August 25-27, 2014 DoubleTree by Hilton Beijing, China
Fagonia cretica L.: Antioxidant potential, protective effects on H O -induced DNA damage and
2 2
identification of polyphenols by RP-HPLC analysis
Samreen Saleem1, Laila Jafri1, Jasia Bokhari1, Nazif Ullah2 and Bushra Mirza1
1Quaid-i-Azam University, Pakistan
2Abdul Wali Khan University, Pakistan
Fagonia cretica L. is a popular folk medicine and it is used with honey in rural areas of Pakistan for its thirst quenching
and blood purifying properties. The present study was designed to determine antioxidant potential and polyphenolic
compounds of F. cretica. Total phenolic and flavonoid contents of F. cretica were estimated and protective effects of plant
extracts on HO-induced DNA damage was determined. Ethyl acetate extract of F. cretica (FCE) contained highest amount of
2 2
phenolics and flavonoids and its antioxidant and protective activities in all tested antioxidant systems were higher than the other
extracts. All extracts of F. cretica showed significant protection in HO-induced DNA damage assay. The RP-HPLC analyses
2 2
provided first ever report regarding the presence of important natural antioxidant compounds such as gallic acid, chlorogenic
acid, rutin, quercitrin and kaempferol in FCE. These results indicate that F. cretica extracts possess potent antioxidant activities
tested in this study and could be used as a potential therapeutic agent in the treatment of diseases involving oxidative stresses.
[email protected], [email protected]
Nat Prod Chem Res 2014 Pharmacognosy-2014 Volume 2, Issue 5
ISSN: 2329-6836, NPCR an open access journal
August 25-27, 2014
Page 132
John A Avbunudiogba et al., Nat Prod Chem Res 2014, 2:5
http://dx.doi.org/10.4172/2329-6836.S1.005
2nd International Conference and Exhibition on
Pharmacognosy, Phytochemistry & Natural Products
August 25-27, 2014 DoubleTree by Hilton Beijing, China
Development and evaluation of liquid oral phytoformulation of Phyllanthus amarus
John A Avbunudiogba1, Christian A Alalor1, Philip F Builders2 and Success Odozie1
1Delta State University, Nigeria
2National Institute for Pharmaceutical Research and Development, Nigeria
Background: The leaves of Phyllanthus amarus (family: Euphorbiaceae) is reported to have goodmedicinal values such as
antitussive properties. However the extract of the plant is verybitter, this constitutes a challenge in formulating an acceptable
oral liquid dosage form.Therefore, the aim of this study is to develop a pleasant tasting liquid preparation of theextract by a
taste masking technique as well as evaluate some physicochemical propertiesof the formulation that relate to its stability.
Methods: Six formulations (A - F) of the extract were prepared. To obtain the most stable andacceptable taste of the herbal syrup
the physicochemical properties such as: Colour, taste, pH, specific gravity, as well as its antioxidant activity were evaluated.
Results and discussion: Formulation C which contains ethanol, citric acid, glycerin and syrup BP as the taste masking agents
was adjudged to have the most acceptable taste and stability. Generally formulations C showed a pH of 6.61 ± 0.02 and 6.62
± 0.04, specific gravity of 1.24 ± 0.02 g/ml and 1.28 ± 0.01 g/ml immediately after formulation and after storage for 10 weeks
respectively.
Conclusion: Formulating P. amarus extract with ethanol, citric acid, glycerin and syrup BPproduced palatable and stable
herbal syrup.
Biography
John A Avbunudiogba holds Masters of Philosophy degree in Pharmaceutics and Pharmaceutical Technology, 2013 (University of Benin, Nigeria); Doctor of
Pharmacy degree (Clinical), 2005, (University of Benin, Nigeria); Master in Business Administration (MBA), 2003 (Delta State University, Abraka, Nigeria) and
Bachelor of Pharmacy degree (BPharm), 1995 (University of Benin, Nigeria). Currently, he is a Lecturer with Delta State University, Abraka, Nigeria, Faculty of
Pharmacy, Department of Pharmaceutics and Industrial Pharmacy. He is also pursuing academic PhD program in Pharmaceutics and Pharmaceutical Technology
at University of Benin, Benin City, Nigeria. He has published more than 15 papers in Pharmaceutics and Phytomedicine in reputed journals.
[email protected]
Nat Prod Chem Res 2014 Pharmacognosy-2014 Volume 2, Issue 5
ISSN: 2329-6836, NPCR an open access journal
August 25-27, 2014
Page 133
Humaira Parveen, Nat Prod Chem Res 2014, 2:5
http://dx.doi.org/10.4172/2329-6836.S1.005
2nd International Conference and Exhibition on
Pharmacognosy, Phytochemistry & Natural Products
August 25-27, 2014 DoubleTree by Hilton Beijing, China
Design, synthesis and biological evaluation of ferrocenyl linked heterocycles as potent antimicrobial agents
Humaira Parveen
University of Tabuk, Saudi Arabia
Infectious diseases caused by bacteria and fungi have emerged as important causes of human morbidity and mortality
worldwide due to their ability to thwart therapeutic regimens by rapidly evolving resistance to antimicrobial agents.
Therefore, design and synthesis of newer, efficacious and safer antimicrobials remain an area of immense significance. As a part
of ongoing program devoted to the search of biologically active heterocycles, twenty four novel ferrocenyl linked sulphur and
nitrogen containing heterocyclic compounds have been synthesized. The structures of all compounds were well characterized
by spectroscopic methods such as IR, NMR, mass and elemental analyses. All newly synthesized compounds were screened for
in vitro antimicrobial activity against the bacterial cultures of Staphylococcus aureus (ATCC-25923), Bacillussubtilis (ATCC-
6635), Salmonella typhimurium (ATCC - 14028), Escherichia coli (ATCC -25922) and fungal cultures of Candida albicans
(ATCC-24433) and Candida krusei (ATCC-6528), by agar well diffusion method. The results of antimicrobial studies revealed
that most of the compounds inhibited growth of bacterial as well as fungal strains. Out of 24 compounds, 16 compounds
emerged as potent antimicrobials. The toxicological studies of these compounds have been studied by MTT assay on PC12-
rat pheochoromocytoma cell line and the results showed that all compounds were non-toxic at a concentration of 100 µM,
indicating that these compounds can acts as novel leads for the development of newer yet safer antimicrobial agents with better
spectrum and potency.
Biography
Humaira Parveen has completed her PhD from Aligarh Muslim University (India) and Postdoctoral studies from Jamia Millia Islamia, New Delhi (India). She also
worked as a Scientist at Department of Chemistry, Jamia Millia Islamia and published a number of research articles in reputed journals. She is also serving as a
reviewer of a number of journals published by Elsevier, John Wiley & Sons, Taylor and Francis Inc., De Gruyter etc.
[email protected]
Nat Prod Chem Res 2014 Pharmacognosy-2014 Volume 2, Issue 5
ISSN: 2329-6836, NPCR an open access journal
August 25-27, 2014
Page 134
Sumi Wijaya et al., Nat Prod Chem Res 2014, 2:5
http://dx.doi.org/10.4172/2329-6836.S1.005
2nd International Conference and Exhibition on
Pharmacognosy, Phytochemistry & Natural Products
August 25-27, 2014 DoubleTree by Hilton Beijing, China
Antibacterial and neuroprotective activities of Mikaniascandens (L.)Willd. (Asteraceae)
Sumi Wijaya1, Ting Kang Nee2, Khoo Teng Jin1 and Christophe Wiart2
1Widya Mandala Catholic University, Indonesia
2The University of Nottingham, Malaysia
The hexane, ethyl acetate and ethanol extract of Mikaniascandens (L.) Willd.were assessed for their antibacterial, antioxidant,
anti-inflammatory and acetylcholinesterase inhibitory activities. The antibacterial activity was evaluated by the pour-plate
disc diffusion assay, the minimum inhibitory concentration, the minimum bactericidal concentration and the death kinetic
assay. The antioxidant activity was evaluated by the ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl
(DPPH) and β-carotene bleaching assays. The anti-inflammatory activity was determined by 5-lipoxygenase inhibitory
activity (5-Lox), meanwhile acetylcolinesterase activity was determined by the TLC-bioautography assay. Among the extracts,
the hexane extract showed the best antibacterial properties. The ethanol extract showed the highest antioxidant and good
5-lipoxygenase inhibitory activity with an IC50 value equal to 23.87 µg/ml. The best acetylcholinesterase inhibitory activity was
elicited by the hexane and ethanol extracts. The total phenolic content values were in the range 106.67 mg/g to 1066.67 mg/g
gallic acid equivalent in the Folin-Ciocalteu assay. The results suggest that Mikaniascandens (L.) Willd.may provide a natural
source of antibacterial, antioxidant, anti-inflammatory and acetylcholinesterase agents.
Biography
Sumi Wijaya works in Widya Mandala Catholic University, Indonesia. She has keen interest in bio constituents from Natural Source.
[email protected]
Nat Prod Chem Res 2014 Pharmacognosy-2014 Volume 2, Issue 5
ISSN: 2329-6836, NPCR an open access journal
August 25-27, 2014
Page 135
Deqiang Dou et al., Nat Prod Chem Res 2014, 2:5
http://dx.doi.org/10.4172/2329-6836.S1.005
2nd International Conference and Exhibition on
Pharmacognosy, Phytochemistry & Natural Products
August 25-27, 2014 DoubleTree by Hilton Beijing, China
The active constituents of yacon leaves
Deqiang Dou, Tingguo Kang and Feng Dong
Liaoning University of Traditional Chinese Medicine, China
Yacon, Smallanthus sonchifolius, which was originally cultivated in the Andean highlands of South America, was introduced
into China via Japan in the 1990s. It has been reported that the tubers of yacon contain a high concentration of oligofructans
and polyphenols, and that its leaf extract has potent antidiabetic effects. To determine the antidiabetic components of the yacon
leaves, 16 compounds were isolated form water and ethanolic extracts of yacon leaves by various kinds of chromatography
methods, such as silica gel, ODS, Sephadex-LH20 column and preparative HPLC. Eight new compounds were characterized as
smaditerpenic acid A (1), smaditerpenic acid B (2), smaditerpenic acid C (3), smaditerpenic acid D (4), ent-kaurane-3β, 16β,
17, 19-tertol (5), ent-kaurane-16β, 17, 18, 19-tertol (6), 4-[(1E)-3-butoxybut-1-en-1-yl]-3, 5, 5-trimethylcyclohex-3-en-1-yl
β-D-glucopyranoside (7) and 5, 8-dihydroxyl-(5H, 8H)-β-ionol (8). Other ingredients were all firstly isolated from the title
genus and they were: ent-kaurane-3β,16β,17-triol (9), ent-kaurane-16β, 17-diol-19-oic acid (10), ent-kauran- 16β, 17, 18-triol
(11), 1-pentacosanol (12), octacosanol (13), 3’, 4’, 5-trihydroxy-3, 7-dimethoxyflavone (14), 3, 4-dihydroxybenzaldehyde (15)
and isorhamnetin (16). The α-glucosidase inhibition activity of smaditerpenic acid-type and kaurane-type compounds were
examined, indicating the inhibition activity of smaditerpenic acid-type compounds are stronger and their intensity is similar
to Acarbose. The contents of chlorogenic acid and smaditerpenic acid A in yacon leaves cultivated in China, Korea, Japan and
Peru was compared, indicating the smaditerpenic acid A in yacon leaves increased concurrently as the latitude. In addition, the
anti-diabetes activity of extracts prepared with different extraction methods was compared.
Biography
Deqiang Dou is a Professor in the College of Pharmacy, Liaoning University of Traditional Chinese Medicine. He received his PhD and MSc in Shenyang
Pharmaceutical University and BSc in Jilin Univeristy and also has two years Post-doctoral experience in Meijo University, Japan and the Rutgers University,
USA. He is the Council member of Tonic Drugs Pharmacology, Chinese Pharmacological Society and Liaoning Pharmaceutical Society etc. More than 20 projects
including national 973, NSFC etc. were undertaken and fulfilled. His current research focuses on the quality, bioactives, new drug development and adverse drugs
reactions of traditional Chinese medicine. He received 5 prizes for the Progress of Science and Technology over Provincial grades were awarded and over 200
papers have been published, of which 50 are published in SCI-indexed journals.
[email protected]
Nat Prod Chem Res 2014 Pharmacognosy-2014 Volume 2, Issue 5
ISSN: 2329-6836, NPCR an open access journal
August 25-27, 2014
Page 136
S N Meena et al., Nat Prod Chem Res 2014, 2:5
http://dx.doi.org/10.4172/2329-6836.S1.005
2nd International Conference and Exhibition on
Pharmacognosy, Phytochemistry & Natural Products
August 25-27, 2014 DoubleTree by Hilton Beijing, China
Evaluation of medicinal properties of Grewia nervosa (Lour.) Panigrahi
S N Meena, S C Ghadi and M K Janarthanam
Goa University, India
Grewia nervosa (Lour) Panigrahi, belonging to the family Malvaceae s.l. is widely distributed along the Western Ghats of
India. Although it has been commonly used in traditional medicine, the medicinal properties have not been scientifically
evaluated. Phytochemical analysis established the presence of phenolic compounds, tannins, alkaloids and saponins in leaves.
The aqueous and methanol extracts from leaves and bark of G. nervosa were investigated for medicinal properties using in
vitro assays. The methanol extract of leaves demonstrated 97.5% inhibition of α-amylase activity. Additionally, the methanol
extract of leaves also demonstrated antioxidant activity (5.41) 0.23 mmol/g, dw) that was higher compared to aqueous extract
(3.32) 0.45 mmol/g, dw). Further, the methanol extract of bark exhibited anti-lipoxygenase activity indicative of its potential to
control inflammatory activity. These results suggest that Grewia nervosa would be a potential source for treatment of diabetes
and its associated complications such as oxidative stress and inflammation.
[email protected]
Nat Prod Chem Res 2014 Pharmacognosy-2014 Volume 2, Issue 5
ISSN: 2329-6836, NPCR an open access journal
August 25-27, 2014
Page 137
Huma Shareef et al., Nat Prod Chem Res 2014, 2:5
http://dx.doi.org/10.4172/2329-6836.S1.005
2nd International Conference and Exhibition on
Pharmacognosy, Phytochemistry & Natural Products
August 25-27, 2014 DoubleTree by Hilton Beijing, China
Hypotensive effect of Cardiospermum halicacabum Linn. in anesthetized rats
Huma Shareef, Ghazala H Rizwani and Ahsana Dar
University of Karachi, Pakistan
In traditional medicine Cardiospermum halicacabum L. (Sapindeaceae) is used against various ailments. The search for a new
remedy, easily available, non-expensive, able to lower hypertension and standardize blood pressure, made the author and her
team to develop an herbal medicine. Crude ethanol extract of C. halicacabum and its various fractions ethyl acetate and butanol
showed a dose-dependent hypotensive effect in anaesthetized rats. The trachea was exposed and freed from connective tissue
and incubated by cannula to facilitate spontaneous respiration. The right carotid artery and left jugular vein were cannulated
with polyethylene tubing PE-50 for monitoring blood pressure changes via pressure transducer (Gould P23 ID) connected to
a Grass model 79D polygraph and for i.v. injection, respectively. Drugs or the plant extracts were administered at a constant
volume of 0.5 ml/kg, followed by injection of 0.2 ml of saline that flushed the cannula. Systolic, diastolic and mean arterial
blood pressure (MABP) was measured in mm Hg and heart rate in beats/min Ethanol extract of C. halicacabum showed a
significant activity at 50 mg/kg dose. Ethyl acetate fraction (10, 20, 30, 40, and 50 mg/kg) induced dose dependent fall in
systolic and diastolic blood pressure, heart rate of rats. At 10-30 mg/kg the hypotensive effect non-significantly reduced by 10-
15%. However, the extract at 40 mg/kg induced significant hypotensive effect calculated as 30.95 ± 3.2% MABP and this effect
persists till 50 mg/kg. The higher polar fraction (butanol) of the whole plant failed to produce any significant response against
MABP at all the tested doses (10-50 mg/kg). C. halicacabum lowers blood pressure, exerts a dose-dependent hypotensive effect,
can be used as hypotensor.
Biography
Huma Shareef has completed her PhD in Pharmacognosy in 2013 from University of Karachi. She is the Associate professor and Head of The department of
Pharmaognosy, Faculty of Pharmacy, Ziauddin University. She has published more than 25 papers in reputed journals.
[email protected]
Nat Prod Chem Res 2014 Pharmacognosy-2014 Volume 2, Issue 5
ISSN: 2329-6836, NPCR an open access journal
August 25-27, 2014
Page 138
Zhang Shidong et al., Nat Prod Chem Res 2014, 2:5
http://dx.doi.org/10.4172/2329-6836.S1.005
2nd International Conference and Exhibition on
Pharmacognosy, Phytochemistry & Natural Products
August 25-27, 2014 DoubleTree by Hilton Beijing, China
Aqueous extract of Bai-Hu-Tang, a classical Chinese herb formula, prevents LPS fever in rabbits
Zhang Shidong and Yan Zuoting
Chinese Academy of Agricultural Sciences, China
Bai-Hu-Tang (BHT) was traditionally used to clear excess heat and promote generation of body fluids.To investigate
the effect and mechanism of BHT, the model of fever syndrome of Chinese medicine pattern was imitated by LPS
injection i.v. in rabbits, and BHT was gavaged. The results showed that there is no striking impact from Baihu Soup to serum
immunoglobulin (IgG, IgA, IgM) and T-AOC in model animals, but SOD was decreased and MDA increased.Thepathologic
observation in liver tissue and serum biochemical analysis was conducted before and after treatment with the BHT. The results
showed that rabbit liver lesion was caused by 15µg/kg LPS strikingly with the levels of serum ALT, AST and Urea increased,
while TC was decreased.After treatment with the formula, BHT prevented sudden increase of IL-10, TNF-α, ALT and AST,
and liver damage induced by LPS. However, there were no significant changes of other cytokines of IL-2, IL-4, and IFN-γ.
BHT also prevented significantly decrease of the percentage of CD8+ T cells since LPS injection, but there was no change of
CD4+ cells percentage. Comparative analyses of gene expression profile in rabbits’liver were analyzed using Agilent cDNA
microarrays. The results demonstrated that genes expression pattern could be clustered into three groups significantly, and
there were 606 up-regulated genes and 859 down-regulated genes in model group, and 106 up-regulated genes and 429 down-
regulated genes in BHT-prevented group. GO annotation indicated that differentially expressed genes were involved in 24
kinds of physiological activities. Among these processes, metabolic process, catabolic process, biosynthetic process, immune
response, and inflammatory process have significant gene expression changes in the course of Qifen Syndrome formation and
BHT prevention. In conclusion, BHT played an important role in immunity protection and anti-injury through preventing
immunoinflammatory damage by LPS.
Biography
Yan Zuoting has completed his PhD at the age of 44 years from Gansu Agriculture University. He is the Director of master degree candidate, a senior professional
Veterinarian. He is good at Chinese traditional veterinary medicine and herbal medicine research. He has published more than 60 papers in reputed journals.
[email protected]
Nat Prod Chem Res 2014 Pharmacognosy-2014 Volume 2, Issue 5
ISSN: 2329-6836, NPCR an open access journal
August 25-27, 2014
Page 139
Magdy M D Mohammed, Nat Prod Chem Res 2014, 2:5
http://dx.doi.org/10.4172/2329-6836.S1.005
2nd International Conference and Exhibition on
Pharmacognosy, Phytochemistry & Natural Products
August 25-27, 2014 DoubleTree by Hilton Beijing, China
Structure-antimutagenicity relationship of anthraquinones
Magdy M D Mohammed
National Research Center, Egypt
Mutagens are not only involved in genotoxicity and carcinogenesis but also involved in the inception and pathogenesis
of several chronic degenerative diseases including hepatic disorders, neurodegenerative disorders, cardiovascular
disorders, diabetes, arthritis, chronic inflammation and in the process of ageing. One of the best ways to minimize the
detrimental effects of mutagens is by the use of natural antimutagens. Naturally occurring antimutagenic principles present in
plants, human diet and other sources have protective effects against mutagens. These include flavonoids, phenolics, coumarins,
carotenoids, anthraquinones, tannins, saponins and many more. The present review attempts to furnish a brief overview on
structure-antimutagenicity relationships of natural and synthetic anthraquinones, to highlight on the structural features of
anthraquinones which accounted for their activity.
Biography
Magdy M D Mohammed has completed his BSc at the age 22 years, and worked at National Research Center as Researcher Assistant by the year 2000. He
finished his MSc in December 2005 and got promoted to the position as Assistant Researcher. He was awarded the PhD degree from Faculty of Science - El-
Menoufia University, Egypt in December 2008. In June 2009 he was granted a German Egyptian Research Short term Scholarship [GERSS] for Post Doctor. He has
been invited as a Visiting Researcher to the University of Southern Denmark - Department of Physics, Chemistry and Pharmacy for six months. He has participated
in many local (Egypt) and international (India) conferences as a speaker. He has published more than 8 papers in reputed journals and serving as excellent reviewer
in different reputed journals dealing with the isolation and structure elucidation of bioactive naturally occurring secondary metabolites from medicinal and terrestrial
plants. His major research area is the isolation and structural elucidation of the different classes of natural occurring compounds, and then studies their biological
activity and finally study the structure activity relationship.
[email protected]
Nat Prod Chem Res 2014 Pharmacognosy-2014 Volume 2, Issue 5
ISSN: 2329-6836, NPCR an open access journal
August 25-27, 2014
Page 140