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“PEST SUCCESSION AND IMPACT OF NITROGENOUS FERTILIZER AND INSECTICIDES ON INCIDENCE OF LEAFHOPPER, Amrasca biguttula biguttula (Ishida) IN OKRA” BY PATHAN NAZIYABANU PARAVEZKHAN B. Sc. (Agri.) DEPARTMENT OF AGRICULTURAL ENTOMOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND – 388 110 2016 (Registration No. 04-2396-2014) “PEST SUCCESSION AND IMPACT OF NITROGENOUS FERTILIZER AND INSECTICIDES ON INCIDENCE OF LEAFHOPPER, Amrasca biguttula biguttula (Ishida) IN OKRA” A THESIS SUBMITTED TO THE ANAND AGRICULTURAL UNIVERSITY IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF Master of Science (AGRICULTURE) IN AGRICULTURAL ENTOMOLOGY BY PATHAN NAZIYABANU PARAVEZKHAN B. Sc. (Agri.) DEPARTMENT OF AGRICULTURAL ENTOMOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND – 388 110 2016 (Registration No. 04-2396-2014) Dedicated to My Beloved Family Abstract PEST SUCCESSION AND IMPACT OF NITROGENOUS FERTILIZER AND INSECTICIDES ON INCIDENCE OF LEAFHOPPER, Amrasca biguttula biguttula (Ishida) IN OKRA Name of student Major Guide Pathan Naziyabanu P. Dr. T.M. Bharpoda DEPARTMENT OF AGRICULTURAL ENTOMOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND – 388 110 ABSTRACT Investigations on succession of major insect pests and impact of nitrogenous fertilizer and insecticides on incidence of leafhopper, A. biguttula biguttula in okra were carried out at Agronomy Farm, B. A. College of Agriculture, Anand Agricultural University, Anand during kharif 2014, summer, 2015 and kharif 2015; whereas residues analysis of flonicamid 50 WP was carried out at the laboratory of All India Network Project on Pesticide Residues, Anand Agricultural University, Anand (Gujarat) during kharif, 2015. Leafhopper population showed two peaks with highly significant positive association of Bright Sunshine Hour (BSS) (r = 0.72**) and maximum temperature (MaxT) (0.70**) during kharif, 2014. Whereas during summer, 2015 and kharif 2015; it was noticed with two peaks. The population aphid showed two peaks during kharif, 2014. During summer, 2015 aphid population showed highly significant negative association with BSS (r = -0.72**). The incidence of whitefly remained low during all three seasons. The highest activity of mite was recorded during 3rd week of October, 4th week of May and 2nd week of September during kharif (2014), summer (2015) and kharif (2015), respectively. Temperature (minimum and maximum), BSS and wind speed (WS) were the important abiotic factors affected the fluctuation of mite population. The activity of mealybug was recorded only during kharif, 2015 with highly positive association of BSS (r = 0.77**) and significant negative association of WS (-0.62*). Fruit damage caused by Helicoverpa armigera showed highly significant negative association with WS (r = -0.73**), MinT (-0.78**) and RH (-0.83**) whereas, BSS (0.77**) and MaxT (0.74**) revealed 2 highly significant positive association during kharif (2014). During kharif (2015), MinT (-0.70*) exerted significant negative association. The activity of Earias vittella as fruit borer was observed in all the seasons. Correlation study revealed that BSS (r = 0.88**) and MaxT (0.86**) exhibited highly significant positive impact whereas, WS (-0.75**) and RH (-0.90**) had highly significant negative association 2 during kharif, 2014. During summer (2015); BSS (0.83**), WS (0.74**), MaxT (0.79**) and MinT (0.85**) showed highly positive significant association with the fruit damage. The activity of spiders was seen in all the three seasons. MaxT, MinT, BSS and WS are important physical factors interfered significantly on the activity of spiders in okra ecosystem. During kharif, 2014; Scymnus sp. showed highly significant association with mite (0.99**) and spiders revealed highly significant association with aphid (0.77**). During kharif, 2015; coccinellids showed significant positive association with aphid (0.88**). Dimethoate 30 EC (0.03%), thiamethoxam 25 WG (0.01%), flonicamid 50 WP (0.015%) and dinotefuran 20 SG (0.008%) was evaluated against leafhopper, A. biguttula biguttula infesting okra grown with three different nitrogenous fertilizer doses viz., N (50 1 kg/ha), N (100 kg/ha) and N (150 kg/ha) as well as N (no nitrogen). 2 3 0 Flonicamid (0.75 leafhoppers/leaf) was found significantly superior by recording the lowest population of pest. Dinotefuran (1.57) was second best insecticide against leafhopper followed by thiamethoxam. Significantly the lowest (2.00) leafhopper population was recorded in N (no fertilizer plots). Treatment N (2.19) and N 0 1 2 (2.26) were at par and the highest (2.42) leafhopper population was observed in N . 3 The different doses of nitrogenous fertilizer and insecticides had no any significant impact on the efficacy of insecticides in okra ecosystem against leafhopper. The treatments also reflected on okra yield. The spray application of flonicamid 50 WP was found Below Determination Limit (BDL) after 10 days. In nutshell, flonicamid 50 WP (0.015%) was found most effective against leafhopper in okra grown under recommended dose of nitrogen i.e. 100 kg/ha. It is advisable to spray flonicamid 50 WP at vegetative stage to avoid residue problem during picking periods. Dr. T. M. Bharpoda Professor Department of Agricultural Entomology B. A. College of Agriculture Anand Agricultural University Anand-388 110 Gujarat (India) C E R T I F I C A T E This is to certify that the thesis entitled “Pest succession and impact of nitrogenous fertilizer and insecticides on incidence of leafhopper, Amrasca biguttula biguttula (Ishida) in okra” submitted by PATHAN NAZIYABANU PARAVEZKHAN (Reg. No. 04-2396-2014) in partial fulfillment of the requirements for the degree of Master of Science in Agricultural Entomology of the Anand Agricultural University is a record of bonafide research work carried out by her under my guidance and supervision. The thesis has not previously formed the basis for the award of any degree, diploma or other title. Place: Anand (T. M. Bharpoda) Date: /06/2016 Major Guide DECLARATION This is to declare that the whole of the research work reported herein for the partial fulfillment of the requirement for the degree of Master of Science in Agricultural Entomology by the undersigned is the result of investigation done by me under the guidance and supervision of Dr. T. M. Bharpoda, Professor, Department of Agricultural Entomology, B. A. College of Agriculture, Anand Agricultural University, Anand and no part of work has been submitted for any other degree so far. Place: Anand (N. P. Pathan) Date: /06/2016 Counter signed by (T. M. Bharpoda) Professor Department of Agricultural Entomology B. A. College of Agriculture Anand Agricultural University Anand-388 110 ACKNOWLEDGEMENT First of all, I passionately pray to the “Al mighty God whose grace and blessing I could be competent to achieve this endeavor. I am indeed conceited to state that my skilled guide granted all his academic assets to explain painstaking plans that allow me to manage all resources to complete my research work in time. Therefore, my first and principal reserved thanks to my scholarly guide Dr. T. M. Bharpoda, Professor, Department of Entomology, B. A. College of Agriculture, AAU, Anand, Gujarat (India). I am also thankful to him for his painstaking efforts in checking this thesis and making it a reality. I would remain indebted forever. I am also undoubtedly thankful to Dr. P. K. Borad, Professor and Head, Department of Entomology, AAU, Anand for his praiseworthy, academic and constructive suggestions throughout the degree programme. I am highly grateful to state my thanks to the members of my Advisory Committee, Dr. N. M. Gohel, Assistant Research Scientist (Plant Pathology), B A C A, AAU, Anand; Dr. D. B. Sisodiya Associate Research Scientist, Arnej, AAU, Anand and Dr. P. R. Vaishnav, Professor and Head, Department of Agricultural Statistics, AAU, Anand and for their valuable guidance and ever willing co-operation. With full respect, I express my heartfelt and special thanks to Dr. D. M. Korat, Associate Director of Research, A. A. U., Anand; and Dr. R. G. Parmar Associate Professor of Plant Pathology, AAU, Anand for their encouragement. I am also thankful to Dr. P. G. Shah, Shri A. R. Patel, Dr. K. D. Parmar and Milanbhai, A I N P on Pesticide Residue, I C A R Unit, A. A. U., Anand, for their guidance and providing all facilities during residue analysis for my research. I acknowledge with thanks the facilities provided by the Director of Research & Dean PG studies, A.A.U., Anand and the Principal, B. A. College of Agriculture, Anand, during the course of my studies. I also wish to place on record my deep sense of appreciation to Dr C. C. Patel Research Scientist, Dr. R. K. Thummar, Assistant Professor, Ku. Meeral Suthar, Assistant Professor, Department of Entomology B A C A, AAU, Anand; Shri N. A. Bhatt, Assistant Resesarch Scientist, Bidi Tobacco Research Station, AAU, Anand, Dr. Manish Dabhi, Assistant Professor, and all the staff members of the Department of Entomology,

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B. A. College of Agriculture, Anand Agricultural University, Anand ige ra. ,. E . vittella. Leafh o p p e r, Ap h id. , W h itefly, Sem iloo p e r, Sp id e rs.
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