Part B: Proteolytic Enzymes

980 Pages·1976·15.785 MB·English

by  Laszlo Lorand (Eds.)

Contributors to Volume XLV
Article numbers are in parentheses following the names of contributors.
Affiliations listed are current.
KINARDNA NAIRASADGAB (25), Department P. L. NAMELOC (2), Biology Department,
of Medicine, University o Pennsyl- Brookhaven Natio~tal Laboratory, -pU
vania School of Medicine, Philadel- ton, New York
phia, Pennsylvania TREBOR W. COLMAN (12, 25), Depart-
DANIEL YDGAB (54), Research Institute ment o Medicine, University of Penn-
ro Pharmaceutical Chemistry, Buda- sylvania Medical School, Philadelphia,
pest, Hungary Pennsylvania
AVE SADARAB (54), Research Institute ro C. G. CURTIS (15), Department o Bio-
Pharmaceutical Chemistry, Budapest, chemistry, University College, ,fidraC
Hungary Wales, United Kingdom
G. H. BAaLOW (17, 18, 20), Biochemistry LUAP S. DAMUS (53), Department o
Laboratory, Abbott Laboratories, caidraC and Thoracic Surgery, Colum-
North Chicago, Illinois bia-Presbyterian Medical Center, New
DENNIS TTERRAB (29), Department of York, New York
lacigoloiB Sciences, University o Den- EARL W. DAVIE (7a, 8, 9, 10), Depart-
ver, Denver, Colorado ment o Biochemistry, University o
DIANA C. TLETRAB (72), Biology Depart- Washington, Seattle, Washington
merit, Brookhaven National Labora- THOMAS DIETL (67, 68), Organische-
tory, Upton, New York Chemisches Institut, Lehrstuhl r~i
SICNARF J. BEHAL (41), Department o Organische Chemie und Biochemie,
Biochemistry, Texas Tech University ,nehcn~iM West Germany
School o Medicine, Lubbock, Texas LEIRBAG R. UAEPARD (38), Department
OLZSAL SSEREB (79), Institut r~i Meeres- of Microbiology, University o Mon-
kunde an der Universitiit Kiel, Kiel, treal, Montreal, Quebec, Canada
West Germany BEN F. SDRAWDE (29), Department o
YEHUDITH BIRK (56, 57, 58, 59, 60, 61, Biology, Reed College, Portland,
62, 63), Department o/ Agricultural nogerO
Biochemistry, Faculty o/ Agriculture, FRANZ FIEDLER (24), Institut r~i Klin-
The Hebrew University of Jerusalem, ische Chemie und Klinische Biochemie,
Rehovot, Israel Universitiit Mi~nchen, Mi~nchen, West
WILLIAM J. YAWKCORB (21), The Depart- Germany
ment o/ Chemistry, The University of EDWIN FINE (73, 74, 75), Institut r~i
Notre Dame, Notre Dame, Indiana Klinische Chemie und Klinische Bio-
DRAWDE J. ,LLORRAC JR. (28), Depart- chemie, der Universitiit Mi~nchen,
ment o/ Zoology, University o/ Mary- Mfinchen, West Germany
land, College Park, Maryland JEAN-MARIE FRERE (51), Service de Mi-
SICNARF J. ONILLETSAC (21, 23), The De- ,eigoloiborc Faculte ed Medecine, Uni-
partment o/Chemistry, The University versit~ ed Liege, Institut de Botanique,
o/ Notre Dame, Notre Dame, Indiana Liege, Belgium
DANA AVOHCE~ (71), Institute o/ cinagrO HANS FRITZ (27, 70, 73, 74, 75, 76, 79),
Chemistry and Biochemistry, Czechos- Institut ru Klinische Chemie und
lovak Acedemy of Sciences, Fleming- Klinische Biochemie der Universitiit
ovo, Czechoslovakia M~nchen, M~nchen, West Germany
SELRAHC G. ENARI-ICOC (7), Department MARbIES FROHNE (42), Physialogisch-
of Immunopathology, Scripps Clinic chemisches Institut der Martin-Luther-
and Research Foundation, La Jolla, Universitiit, Halle-Wittemberg, Ger-
ainrofilaC many
CONTRIBUTORS TO VOLUME XLV xi
KAzuo AWAKISUF (8, 10), Department o der Universitiit Miinchen, M(tnchen,
Biochemistry, University o Washing- West Germany
ton, Seattle, Washington THEO NNAMFOH (35, 49), Department o
ARABRAB C. FURIE (16), Hematology Biochemistry, University o Toronto,
Section, Department o Medicine, Toronto, Ontario, Canada
Tuts-New England Medical Center IKAADAS AGANAWI (37, 78), Division o
and Tuts University Medical School, Plasma Proteins, Institute ro Protein
Boston, Massachusetts Research, Osaka University, Suita,
ECURB FURIE (16), Hematology Section, Osaka, Japan
Department o Medicine, Tuts-New NOYLOJ JESTY (11), Department o
England Medical Center and Tuts Medicine--Hematology, State Uni-
U~dversity School o Medicine, Boston, versity o New York, Health Sciences
Massachusetts Center, Stony Brook, New York
G. ECIRUAM REHCUAG (34), Biochemistry E. T. KAISER (1), Departments o Bio-
Group, Department o Chemistry, The chemistry and Chemistry, University
University o Calgary, Calgary, Al- o Chicago, Chicago, Illinois
berta, Canada ECIRTAEB LLESSAK (36), Department o
EIRAM-NAEJ GHUYSEN (51), Service de Biochemistry, Medical College o Wis-
Microbiologie, Faculte de Medecine, consin, Milwaukee, Wisconsin
Universit5 de Ligge, Institut de HrsAo IC~TO (7a), Department o Bio-
Botanique, Lidge, Belgium chemistry, University o Washington,
OLZSAL GRA~ (54), Research Institute for Seattle, Washington
Pharmaceutical Chemistry, Budapest, TARFE RELSSEK (45), Eye and Ear Hos-
Hu~gary pital, University o Pittsburgh School
LEwis J. ENEERG (72), Biology Depart- o Medicine, Pittsburgh, Pennsylvania
ment, Brookhaven National Labora- CNEREF J. YDZEK (1, 64), Department o
tory, Upton, New York Biochemistry, University o Chicago,
JOHN H. GRIFFIN (7), Department o Chicago, Illinois
Immunopathology, Scripps Clinic and VROMAM KIKUC~I (40), Noda Institute
Research Foundation, La olla, CaIi- or Scientific Research, Noda-shi,
ornia Chiba-Ken, Japan
L. S. HALL (17), Biochemistry Labora- YRNEH S. KINGI)ON (14), Dental Re-
tory, Abbott Laboratories, North search Center and the Departments
Chicago, Illinois o Medicine and Biochemistry, Uni-
TSROH HANSON (42), Physiologisch- versity o North Carolina School
chemisches Institut der Martin-Luther- o Medicine, Chapel Hill, North
UniversitSt, Halle-Wittenberg, Ger- Carolina
many GNINNEH REYEMRETSOLK (3), Chemisches
PETEa C. ItARPEL (52, 65), Department Dlslitut der BundesanstaIt fi~r Milch-
o Medicine, Division o Hematology, orschung, Kiel, Germany
The New York Hospital-Cornell Medi- OKIHEKAT KOIDE (7a), Department o
cal Center, New York, New York Biochemistry, University o Washing-
RIKIMARU HAYnSHI (48), Research In- ton, Seattle, Washington
stitute for Food Science, Kyoto Uni- KAJ KRESCI (70), Kinderpoliklinik der
versity, Uji, Kyoto, Japan Universitiit Mi~nchen, Mi~nchen, West
TREBOR L. NOSKIR'~IEH (64), Department Germany
o Biochemistry, University o Chicago, H. G. ,MAHTAL JR. (2), Biology Depart-
Chicago, Illinois ment, Brookhaven National Labora-
LRAK RESSARTSHCOH (76, 77), Biochemis- tory, Upton, New York
ches Laboratorium der HNO-Klirlik, KRAM E. ZA;:(EL (9), Department o Bio-
xii CONTRIBUTORS TO VOLUME XLV
chemistry, University o Washington, ORIHCINEG OSHIMA (37), Division o
Seattle, Washington Plasma Proteins, Institute ro Protein
M~bInh LEYH-BouILLE (51), Service de Research, Osaka University, Suita,
MicrobioIogie, Faculte de Medecine, ,akasO Japan
Universitd de Libge, Institut de Bota- I-ITEBAZILE K. NOSRETTAP (30, 31), The
nique, Ligge, Belgium Institute ro Cancer Research, Fox
GWY~nE tI. LITTLE (41), Department Chase Cancer Center, Philadelphia,
o Biochemistry, Texas Tech University Pennsylvania
School o Medicine, Lubbock, Texas DLO~AH R. PERXINS (51), Department o
L. ONAROL (4, 15), Department o Bio- Microbiology, University o Liverpool,
chemistry and Molecular Biology, Liverpool, United Kingdom
Northwestern University, Evanston, TORBEn KEABELLE PE~ERS0N (54), De-
Illinois partment o Molecular Biology, Uni-
REGOR L. DALRDNUL (14), Dental Research versity o Aarhu~, Aarhus, Denmark
Center and the Departments o Bio- SECNARF AnN PITUCK (5), Department
chemistry and Pathology, University o Internal Medicine, Yale University
Jo North Carolina School o Medicine, School o Medicine, New Haven, Con-
Chapel Hill, North Carolina necticut
RENREW TDIELHCAM (79), lnstitut r~if K. L. IXSOKALOP (26), Department o
Physiologische Chemie und Physikal- Obstetrics and Gynecology, Washington
ische Biochemie der t"6tisrevinU -nt'iM University School o Medicine, St.
chen, Miinchen, West Germany Louis, Missouri
nAFFATS MACnUSSON (54), Department JoHn M. TTOCSERP (32, 33, 44), Depart-
o Molecular Biology, University o meat o Biochemistry and Biophysics,
Aarhus, ,st~hraA Denmark Texas A & M University College Sta-
KEnnETH G. MANn (13, 14), Section o tion, Texas
Hematology Research, Mayo Clinic MEaTON H. SLOBVP (72), Department
and Foundation, Rochester, Minnesota o Animal Sciences, Washington State
SICNARF S. ,DNALKRAM JR. (19), Depart- University, Pullman, Washington
ment Jo Biological Chemistry, Univer- TREBOR EFFILCDAR (6), Hematology Re-
sity Jo Southern ,ainroJilaC School Jo search Laboratory, Oklahoma Medical
Medicine, and Cancer Center, Los An- Foundation, Oklahoma City, Okla-
geles, ainroJilaC homa
TAKASm II-ICARUM (39), Department Jo ERNST H. SEDREMIER (3), Chemisches
Clinical Science, Kyoto University, Institut, der Bundesanstalt fir Milch-
Faculty Jo Medicine, Sakyoku, Kyoto, ,gnuhcsro Kiel, Germany
Japan HTENNEK C. ROBBINS (22), Michael
tozo ATIRAN: (46), Institute ro Protein Reese Blood Center Chicago, Illinois
Research, Osaka University, Suita G. ROnCARI (43), Institut ru Molekul-
Osaka, Japan arbiologie und Biophysik, Eidgenos-
ELAY NOSREMEN (5, 6, 11), Department sische Technische Hochschule, Zurich-
o Medicine--Hematology, State Uni- Honggerberg, Switzerland
versity o New York, Health Sciences TREBOR D. ~REBNESOR (53), Department
Center, Stony Brook, New York o Medicine, Harvard Medical School,
LEUNAM NIETO (51), Centro ed Investi- and Beth Israel Hospital, Boston,
senoicag ,sacigoloiB Instituto de Biolo- Massachusetts
aig Celular, Madrid, Spain ~(ENJI II-ICUGAKAS (40), Mitsubishi-Kasei
C. NOLAn (17), Biochemistry Laboratory, Institute o Lie Sciences, Minamicoya,
Abbott Laboratories, North Chicago, Machiada-shi, Tokyo, Japan
Illinois HANS SCHmSSLEa (75), Institut rt~ Kli-
CONTRIBUTORS TO VOLUME XLV Xlll oo.
nische Chemie und Klinische Bio- DLARAH EI-ICSEHCST (67, 68, 69, 74),
chemie, der Universit~t M ,nehent~ Institut r~iJ Organische Chemie, Leh~-
Mi~nchen, West Germany stuhl iir Organische Chemie und Bio-
RETEID-FLOW ONINUELI.ICS (27), Institut chemie, Technische Universitiit Mi~n-
rt~ Klinische Chemie und Klinische chen, M~tnchen, West Germany
Biochemie, der Universit~t M~nchen, SusvMu AWASANUST (46), Institute roJ
Mi~nchen, West Germany Protein Research, Osaka University,
E. N. SHAW (2), Biology Department, Suita, Osaka, Japan
Brookhaven National Laboratory, Up- OAMAt} AWAZEMU (55), Microbial Chem-
ton, New York istry Research Foundation, Institute o
DLAREG E. ,GNIRFEIS JR. (21), The Depart- Microbial Chemistry, Tokyo, Japan
ment o Chemistry, The University OERrl W. RENOAW (32), Department of
o Notre Dame, Notre Dame, Indiana Biochemistry and Nutrition, Univer-
SEMAJ M. ZTEDOS (21, 23), The Depart- sity o Nebraska, Lincoln, Nebraska
ment Jo Chemistry, The University RETEP I-I. DRAW (36), Department o Bio-
o Notre Dame, Notre Dame, Indiana chemistry, Medical College of Wiscon-
WILLIS L. SENRATS (41), Department of sin, Milwaukee, Wisconsin
Biochemistry, Texas Tech University
TREBOR M. G~EBNIEW (12), Department
School o Medicine, Lubbock, Texas
o Medicine, Harvard Medical School,
M. I.ICVRNIETS (66), Centre National de
Boston, Massachusetts
Transfusion Sanguine, Paris, France
ALLETS ~I. WILKES (33, 44), Department
KENNETH J. NOSNEVETS (34), Biochemis-
of Biochemistry and Biophysics, Texas
try Group, Department o Chemistry,
A & M University, College Station,
The University Jo Calgary, Calgary,
Texas
Alberta, Canada
CHRISTINE L. TI.IGIRW (36), Department
K. R~XCOTS (18), Pentapharm, Ltd., Basle,
o Biochemistry, Medical College o
Switzerland
Wisconsin, Milwaukee, Wisconsin
E. LLOTS (43), Institut rt~ Molekularbi-
TREG REREDNUW (79), Institut rt~J Klin-
ologie und Biophysik, Eidgenossische
ische Chemie und Klinische Biochemie
Technische Hochschule, Zurich-Hong-
der Universitiit Mi~nehen, Mi~nchen,
gerberg, Switzerland
West Germany
Louis AIRAMMUS (22), Departments of
ARIEH YnRON (45, 50), Department o
Medicine and Pathology, Pritzker
Biophysics, The Weizmann Institute
School o Medicine, The University
o Science, Rehovot, Israel
o Chicago, Chicago, Illinois
Tor~oJI SczcxI (37, 78), Division o L. J. D. DLEVENAZ (26), Department o
Physiology, University o Illinois at
Plasma Proteins, Institute ToJ Protein
the Medical Center, School o Medi-
Research, Osaka University, Suita,
cine, Chicago, Illinois
Osaka, Japan
UBONEDIt'I TAKAHASHI (78), Division o H. RERUL (43, 47), Institut r~i Molekular-
Plasma Proteins, Institute or Protein biologie und Biophysik, Eidgenossische
Research, Osaka University, Suita, Technische Hochschule, Zurich-Hong-
Osaka, Japan gerberg, Switzerland
Preface
This volume on "Proteolytic Enzymes," as the previous one (Volume
XIX) in the Methods in Enzymology series, was to have been coedited
with the late Dr. Gertrude E. Perlmann of The Rockefeller University.
Actually, she still participated in much of its organization, diligently
sending out letters even from the hospital during the summer of 1974,
but, alas, she could not see the task completed. In her passing, I lost a
personal friend and an excellent collaborator. My colleagues will surely
share my feelings in dedicating this volume to her memory.
Proteolytic enzymes are assuming increasing significance in biological
regulation. A published account of a symposium held in the fall of 1974,
entitled "Proteases and Biological Control" (E. Reich, D. B. Rifkin,
and E. Shaw, eds.). Cold Spring Harbor, New York, 1975, presents
ample evidence that proteolytic enzymes serve as key agents in many
intra- and extracellular phenomena. This is a far cry from the days when
proteases were considered merely useful tools for protein degradation in
nutrition and for protein sequencing. The delicately coordinated systems
of the blood coagulation cascade, fibrinolysis, kinin generation, comple-
ment activation, fertilization of eggs, cell migration, and mitogenic trans-
formations are but a few of the outstanding examples. In this volume an
effort was made to cover rather extensively the enzymes involved in
blood coagulation and fibrinolysis and to include the great variety of
naturally occurring inhibitors. In general, it is now clear that the field
of protease inhibitors is becoming just as exciting as that of the enzymes
themselves.
Special thanks are due to Dr. Joyce Bruner-Lorand and to Mrs.
Pauline Velasco for their constant help.
Laszlo Lorand
XV
GERTRUDE E. PERLMANN
METHODS IN ENZYMOLOGY
EDITED YB
Sidney P. Colowick and Nathan 0. Kaplan
VANDERBILT UNIVERSITY DEPARTMENT OF CHEMISTRY
SCHOOL OF MEDICINE UNIVERSITY OF CALIFORNIA
NASHVILLE~ TENNESSEE AT SAN DIEGO
LA JOLLA~ CALIFORNIA
I. Preparation and Assay of Enzymes
II. Preparation and Assay of Enzymes
III. Preparation and Assay of Substrates
IV. Special Techniques for the Enzymologist
V. Preparation and Assay of Enzymes
VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
VII. Cumulative Subject Index
ivx
METHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
Sidney P. Colowick Nathan .O Kaplan
EMULOV VIII. Complex Carbohydrates
Edited by HTEBAZILE F. DLEFUEN DNA ROTCIV GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited by WILLIS A. DOOW
EMULOV X. Oxidation and Phosphorylation
Edited by DLANOR W. KOORBATSE DNA DRANYAM E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited by C. H. W. SRIH
EMULOV XII. Nucleic Acids (Parts A and B)
Edited by ECNERWAL NAMSSORG DNA EIVIK EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited by J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited by DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited by HTENNEK NITSUK
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by TREBREH ROBAT DNA CELIA WHITE ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited by DLANOD B. KCIMROCCM DNA LEMUEL D. WRIGHT
~MULOV XIX. Proteolytic Enzymes
Edited by EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
iivx
XVlll .°° METHODS IN ENZYMOLOGY
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by MAILLIW B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXII. Biomembranes (Part B)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by AHTRAM G. SINNED DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
METHODS IN ENZYMOLOGY xix
EMULOV XXXV. Lipids (Part B)
Edited by JOHN M. NIETSNEWOL
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by TREB W. YELLAM'O DNA LEOJ G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by TREB W. YELLAM'O DNA LEOJ G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by LEOJ G. NAMDRAH DNA TREB W. YELLAM'O
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and
Organ Systems)
Edited by LEOJ G. NAMDRAH DNA TREB W. YELLAM'O
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by TREB W. YELLAM'O DNA LEOJ G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part )B
Edited by W. A. DooW
EMULOV XLII. Carbohydrate Metabolism (Part )C
Edited by W. A. DOOW
EMULOV XLIII. Antibiotics
Edited by JOHN H. HSAH
EMULOV XLIV. Immobilized Enzymes
Edited by SUALK HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited by OLZSAL DNAROL