P/ACE™ MDQ Installation and Maintenance Manual 32 - AB Sciex

108 Pages·2012·4.48 MB·English

Instructions For Use
P/ACE MDQ
Installation & Maintenance
32 Karat 8.0
A36419AC
March 2012
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821
P/ACE MDQ Installation & Maintenance
32 Karat 8.0
PN A36419AC (March 2012)
© 2012 Beckman Coulter, Inc.
All Rights Reserved
Printed in the USA
Made in the USA
Trademarks
BECKMAN COULTER, the stylized logo, P/ACE, 32 Karat,
and MDQ are trademarks of Beckman Coulter, Inc., and
are registered with the USPTO.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Find us on the World Wide Web at:
www.beckmancoulter.com
Table of Contents
Section 1 Introduction
General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Section 2 Specifications and Site Requirements
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Section 3 Hardware and Software Installation
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Installation - General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Unpacking and Repacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Repacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Installation - Hardware / Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Installing the GPIB Communication Interface Board . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Configuring the Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Power/Peripherals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
CMOS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Boot Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Installing the GPIB Communication Interface Board Driver . . . . . . . . . . . . . . . . . . . . 11
Installing the 32 Karat Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Software Licenses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Installing the Software License Key . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Changing the Network Name or Workstation Operating System . . . . . . . . . . . . . . . . . . 15
Installing the P/ACE MDQ Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Installing the UV or PDA Detector/Capillary Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . 16
Installing the Capillary Cartridge for UV/PDA Detectors . . . . . . . . . . . . . . . . . . . . . . . . . 19
Installing the LIF Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Pre-installation Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Bench Space . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Installing the Interconnect Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Connecting the Laser Cable(s) to the Interconnect Module . . . . . . . . . . . . . . . . . . . . 21
Installing the LIF Detector Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Installing Filters in the Filter Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Installing the Filter Housing into the Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Installing the LIF Detector into the P/ACE MDQ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Preparing the Cartridge for LIF Usage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Installing the LIF Plug and Probe Stabilizer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Connecting the Probe to the Clamping Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Connecting the Computer to the P/ACE MDQ Instrument . . . . . . . . . . . . . . . . . . . . . . . 27
Analog Outputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
PDA Detector Outputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
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UV Detector Outputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
LIF Detector Outputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Configuring the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Installing the Buffer/Sample Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Installation - Additional Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Buffer Tray Reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
External Detector Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Section 4 Maintenance
Maintenance Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Rebuilding the Capillary Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Removing the Capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Replacing the Coolant Tubing and Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Marking the Capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Installing the Aperture Clip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Trimming the Capillary Ends . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Filling Vials and Installing Caps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Filling 0.5 mL Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Filling the 2.0 mL Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Filling PCR Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Filling the 96-Well Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Installing the UV Detector Wavelength Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Calibrating the PDA Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Changing the Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Cleaning the Fiber Optics (UV and PDA Detectors) . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
LIF Care and Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Inspecting the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Calibrating the LIF Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Accessing the LIF Calibration Wizard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Manual Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Auto Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Calibration Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Capillary Diameter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Instrument Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Cleaning the Interface Block, Ejectors and Electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Refilling the Coolant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Replacing the Electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Replacing the Quad-Rings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Replacing the Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
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Section 5 Additional Features
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Connecting Detectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Connecting (Remote Start) Trigger Signals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Trigger Start of Data Acquisition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Hold Off Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Stop Data Acquisition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Outputs 1 & 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Outputs 3 & 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Connecting External Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
SS420X External Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Installing the SS420X External Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Connecting Detectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Connecting Start Inputs (Trigger Signals) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Trigger Start of Data Acquisition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Hold Off Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Stop Data Acquisition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Outputs 1 & 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Outputs 3 through 8 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Connecting External Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Configuring the SS420X External Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
P/ACE™ MDQ External Detector Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Initial Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Installing the Capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Installing the Capillary in the Standard Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Installing the Capillary in the EDA-Only Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Installing the Cartridge in the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Programming for EDA Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Connecting to an External Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Additional Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
P/ACE MDQ™ MDQ Buffer Reservoir Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Filling the Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Installing the Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Programming the Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Solvent Compatibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
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Warranty
Warranty
Subject to the exceptions and upon the conditions stated below, the Company warrants that the
products sold shall be free from defects in workmanship and materials for one year after delivery
of the products to the original Purchaser by the Company and if any such product should prove to
be defective within such one year period, the Company agrees, at its option, either (1) to correct
by repair or at the Company's election by replacement, any such defective product provided that
investigation and factory inspection discloses that such defect developed under normal and
proper use, or (2) to refund the purchase price.
The exceptions and conditions mentioned above are as follows:
a. Components or accessories manufactured by the Company which by their nature are
not intended to and will not function for one year are warranted only to give reasonable
service for a reasonable time. What constitutes a reasonable time and a reasonable
service shall be determined solely by the Company. A complete list of such
components and accessories is maintained at the factory.
b. The Company makes no warranty with respect to components or accessories not
manufactured by it. In the event of defect in any such component or accessory the
Company will give reasonable assistance to Purchaser in obtaining from the respective
manufacturer whatever adjustment is authorized by the manufacturer's own warranty.
c. Any component claimed to be defective must, if required by the Company, be returned
to the factory, transportation charges prepaid, and will be returned to Purchaser with
transportation charges collect unless the product is found to be defective, in which case
the Company will pay all transportation charges.
d. If the product is a reagent or the like, it is warranted only to conform to the quantity
and content and for the period (but not in excess of one year) stated on the label at the
time of delivery.
e. The Company may from time to time provide a special printed warranty with respect to
a certain product and where applicable, such warranty shall be deemed incorporated
herein by reference.
IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF
ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY
AND THAT THE COMPANY SHALL HAVE NO LIABILITY FOR SPECIAL OR
CONSEQUENTIAL DAMAGES OF ANY KIND OR FROM ANY CAUSE WHATSOEVER
ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR,
MAINTENANCE, OR REPLACEMENT OF ANY OF THE PRODUCTS SOLD.
Representations and warranties made by any person, including dealers and representatives of the
Company, which are inconsistent or in conflict with the terms of this warranty, shall not be
binding upon the Company unless reduced in writing and approved by an expressly authorized
officer of the Company.
P/ACE MDQ Installation & Maintenance Manual
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Warranty
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Safety Information
Safety Information
This section provides safety information and instructions for the hardware and accessories of the
system. It is broken down into the following subsections:
r Symbols
r Waste Management and Recycling
r Chemical and Biological Safety
r Electrical Safety
r Moving Parts Safety
P/ACE MDQ Installation & Maintenance Manual
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Safety Information
Safety Symbols
Safety Symbols
The following information describes the notes and warnings used in this document. Beckman
Coulter recommends that you review this information before using the system.
WARNING Paragraphs marked by “WARNING” alert you of a potential hazard to your personal safety if you
do not adhere to the information stated within the paragraph.
CAUTION Paragraphs marked by “CAUTION” indicate that there is a potential danger of equipment damage.
IMPORTANT Paragraphs marked by “IMPORTANT” contain information about a possible software program
failure, draw attention to a specific software setting or point out that a loss of data may occur if information
stated within the paragraph is not adhered to or if procedures are executed incorrectly.
NOTE Paragraphs marked by “NOTE” contain supplemental or explanatory information concerning the
current topic or procedural step.
The symbols displayed below and on the instrument are reminders that all safety instructions
should be read and understood before installation, operation, maintenance, or repair to this
instrument is attempted.
CAUTION This icon accompanies text and/or other symbols dealing with potential
damage to equipment. When present, it indicates that there is a potential danger of
equipment damage, software program failure, or that a loss of data may occur if
information stated within the “CAUTION” paragraph is not adhered to or procedures
are executed incorrectly.
HIGH VOLTAGE Paragraphs marked by this symbol indicate that a potential hazard to
your personal safety exists from a high voltage source.
BIOHAZARD Paragraphs marked by this symbol indicate that a potential hazard to
your personal safety exists from a biological source.
LASER LIGHT Paragraphs marked by this symbol indicate that a potential hazard to
your personal safety exists from a laser source.
SHARP OBJECTS Paragraphs marked by this symbol indicate that a potential hazard
to your personal safety exists from unblunted corners or other appendages on the
outside or inside of the equipment.
HOT SURFACE Paragraphs marked by this symbol indicate that a potential hazard to
your personal safety exists from heated surfaces or other appendages on the outside
or inside of the equipment.
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