Organ Regeneration: 3D Stem Cell Culture & Manipulation

248 Pages·2017·13.7 MB·English

by  Takashi Tsuji (eds.)

Methods in
Molecular Biology 1597
Takashi Tsuji Editor
Organ
Regeneration
3D Stem Cell Culture
& Manipulation
M M B
ethods in olecular iology
Series Editor
John M. Walker
School of Life and Medical Sciences
University of Hertfordshire
Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes:
http://www.springer.com/series/7651
Organ Regeneration
3D Stem Cell Culture & Manipulation
Edited by
Takashi Tsuji
Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan
Organ Technologies Inc., Tokyo, Japan
Editor
Takashi Tsuji
Laboratory for Organ Regeneration
RIKEN Center for Developmental Biology
Kobe, Hyogo, Japan
Organ Technologies Inc.
Tokyo, Japan
ISSN 1064-3745 ISSN 1940-6029 (electronic)
Methods in Molecular Biology
ISBN 978-1-4939-6947-0 ISBN 978-1-4939-6949-4 (eBook)
DOI 10.1007/978-1-4939-6949-4
Library of Congress Control Number: 2017933953
© Springer Science+Business Media LLC 2017
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Dedication
This book is dedicated to the memory of Yoshiki Sasai, a scientist who made a great contri-
bution to the advancement of developmental biology.
v
Preface
Organogenesis is a complex process that involves tissue self-organization, cell-cell interac-
tions, regulations of cell signaling molecules, and cell movements. During embryonic
development, organ-forming fields are organized in a process depending on the body plan.
Various lineages of stem cells are produced and play central roles in organ development. In
recent years, stem cell researchers have made advances in various aspects of three- dimensional
organogenesis including cell growth, differentiation, and morphogenesis. Studies using
multipotent stem cells have provided knowledge of the complex pattern formation and tis-
sue self-organization during embryogenesis.
Stem cell research not only promotes basic biology but also can aid the development of
regenerative medicine as a potential future clinical application. The current approaches to
developing future regenerative therapies are influenced by our understanding of embryonic
development, stem cell biology, and tissue engineering technology. To restore the partial
loss of organ function, stem cell transplantation therapies were developed for several dis-
eases such as hematopoietic malignancies, Parkinson’s disease, myocardial infarction, and
hepatic insufficiency. The next generation of regenerative therapy will be the development
of fully functioning bioengineered organs that can replace lost or damaged organs follow-
ing disease, injury, or aging. It is expected that bioengineering technology will be devel-
oped to reconstruct fully functional organs in vitro through the precise arrangement of
several different cell types.
In recent years, significant advances in techniques for organ regeneration have been
made using three-dimensional stem cell culture in vitro. Several groups recently reported
the generation of neuroectodermal and endodermal organs via the regulation of complex
patterning signals during embryogenesis and self-formation of pluripotent stem cells in
three-dimensional (3D) stem cell culture. Other groups attempted to generate functional
organs that develop by reciprocal epithelial and mesenchymal interactions using embryonic
organ inductive stem cells. Several groups reported the generation of three-dimensional
mini-organs/tissues by the reproduction of stem cells and their niches. These studies pro-
vide a better understanding of organogenesis in developmental biology and open possibili-
ties for methodologies to be used in next-generation organ regenerative therapy.
Here, we focus on recent studies of organ regeneration from stem cells using in vitro
three-dimensional cell culture and manipulation. These protocols have led both basic and
clinical researchers to face new challenges in the investigation of organogenesis in develop-
mental biology in order to develop applications for next-generation regenerative therapies.
I sincerely thank all of the authors for their contributions. I am also grateful to Dr. John
Walker, the Editor in Chief of the MIMB series, for his continued support. I also thank
Patrick Martin and Yasutaka Okazaki, Editors of the Springer Protocol series.
Kobe, Hyogo, Japan Takashi Tsuji
vii
Contents
Dedication....................................................... v
Preface.......................................................... vii
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
1 Generation of Various Telencephalic Regions from Human Embryonic
Stem Cells in Three-Dimensional Culture............................ 1
Taisuke Kadoshima, Hideya Sakaguchi, and Mototsugu Eiraku
2 Generation of a Three-Dimensional Retinal Tissue from Self-Organizing
Human ESC Culture ........................................... 17
Atsushi Kuwahara, Tokushige Nakano, and Mototsugu Eiraku
3 3D Culture for Self-Formation of the Cerebellum from Human
Pluripotent Stem Cells Through Induction of the Isthmic Organizer........ 31
Keiko Muguruma
4 Reconstitution of a Patterned Neural Tube from Single Mouse
Embryonic Stem Cells........................................... 43
Keisuke Ishihara, Adrian Ranga, Matthias P. Lutolf, Elly M. Tanaka,
and Andrea Meinhardt
5 Functional Pituitary Tissue Formation............................... 57
Chikafumi Ozone and Hidetaka Suga
6 Directed Differentiation of Mouse Embryonic Stem Cells
Into Inner Ear Sensory Epithelia in 3D Culture........................ 67
Jing Nie, Karl R. Koehler, and Eri Hashino
7 Generation of Functional Thyroid Tissue Using 3D-Based Culture
of Embryonic Stem Cells......................................... 85
Francesco Antonica, Dominika Figini Kasprzyk, Andrea Alex Schiavo,
Mírian Romitti, and Sabine Costagliola
8 Functional Tooth Regeneration.................................... 97
Masamitsu Oshima, Miho Ogawa, and Takashi Tsuji
9 Functional Hair Follicle Regeneration by the Rearrangement
of Stem Cells.................................................. 117
Kyosuke Asakawa, Koh-ei Toyoshima, and Takashi Tsuji
10 Functional Salivary Gland Regeneration ............................. 135
Miho Ogawa and Takashi Tsuji
11 Generation of a Bioengineered Lacrimal Gland by Using the Organ
Germ Method................................................. 153
Masatoshi Hirayama, Kazuo Tsubota, and Takashi Tsuji
12 Generation of Gastrointestinal Organoids from Human Pluripotent
Stem Cells.................................................... 167
Jorge O. Múnera and James M. Wells
ix
x Contents
13 Generation of a Three-Dimensional Kidney Structure from Pluripotent
Stem Cells.................................................... 179
Yasuhiro Yoshimura, Atsuhiro Taguchi, and Ryuichi Nishinakamura
14 Making a Kidney Organoid Using the Directed Differentiation
of Human Pluripotent Stem Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
Minoru Takasato and Melissa H. Little
15 Liver Regeneration Using Cultured Liver Bud......................... 207
Keisuke Sekine, Takanori Takebe, and Hideki Taniguchi
16 Formation of Stomach Tissue by Organoid Culture Using Mouse
Embryonic Stem Cells........................................... 217
Taka-aki K. Noguchi and Akira Kurisaki
17 In Vivo Model of Small Intestine .................................. 229
Mahe M. Maxime, Nicole E. Brown, Holly M. Poling,
and Helmrath A. Michael
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247
Contributors
Francesco antonica • Institute of Interdisciplinary Research in Molecular Human
Biology (IRIBHM), Université Libre de Bruxelles, Brussels, Belgium; Department
of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK
KyosuKe asaKawa • Laboratory for Organ Regeneration, RIKEN Center for
Developmental Biology, Kobe, Hyogo, Japan
nicole e. Brown • Department of Pediatric General and Thoracic Surgery, Cincinnati
Children’s Hospital Medical Center, Cincinnati, OH, USA
saBine costagliola • Institute of Interdisciplinary Research in Molecular Human Biology
(IRIBHM), Université Libre de Bruxelles, Brussels, Belgium
Mototsugu eiraKu • In Vitro Histogenesis team, RIKEN Center for Developmental
Biology, Kobe, Hyogo, Japan
eri HasHino • Department of Otolaryngology—Head and Neck Surgery, and Stark
Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis,
IN, USA
MasatosHi HirayaMa • Department of Ophthalmology, Keio University School of Medicine,
Tokyo, Japan
KeisuKe isHiHara • DFG Research Center for Regenerative Therapies Dresden, Technische
Universität Dresden, Dresden, Germany
taisuKe KadosHiMa • Cell Asymmetry team, RIKEN Center for Developmental Biology,
Kobe, Hyogo, Japan; Asubio Pharma Co ., Ltd ., Kobe, Hyogo, Japan
doMiniKa Figini KasprzyK • Institute of Interdisciplinary Research in Molecular Human
Biology (IRIBHM), Université Libre de Bruxelles, Brussels, Belgium
Karl r. KoeHler • Department of Otolaryngology—Head and Neck Surgery, and Stark
Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis,
IN, USA
aKira KurisaKi • Graduate School of Life and Environmental Sciences, The University
of Tsukuba, Tsukuba, Ibaraki, Japan; Research Institute for Drug Discovery, National
Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki,
Japan
atsusHi KuwaHara • Laboratory for In Vitro Histogenesis, RIKEN Center for
Developmental Biology, Chuo, Kobe, Japan; Regenerative and Cellular Medicine Office,
Sumitomo Dainippon Pharma Co ., Ltd ., Chuo, Kobe, Japan; Environmental Health
Science Laboratory, Sumitomo Chemical Co ., Ltd ., Osaka, Japan
Melissa H. little • Murdoch Children’s Research Institute, Parkville, VIC, Australia;
Department of Pediatrics, University of Melbourne, Parkville, VIC, Australia
MattHias p. lutolF • Laboratory of Stem Cell Bioengineering, Institute of Bioengineering,
School of Life Sciences and School of Engineering, Ecole Polytechnique Fédérale de
Lausanne (EPFL), Lausanne, Switzerland; Institute of Chemical Sciences and
Engineering, School of Basic Science, EPFL, Lausanne, Switzerland
MaHe M. MaxiMe • Department of Pediatric General and Thoracic Surgery, Cincinnati
Children’s Hospital Medical Center, Cincinnati, OH, USA; Department of Pediatrics,
University of Cincinnati, Cincinnati, OH, USA
xi
xii Contributors
andrea MeinHardt • DFG Research Center for Regenerative Therapies Dresden,
Technische Universität Dresden, Dresden, Germany
HelMratH a. MicHael • Department of Pediatric General and Thoracic Surgery,
Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA; Department
of Pediatrics, University of Cincinnati, Cincinnati, OH, USA
KeiKo MuguruMa • Laboratory for Cell Asymmetry, RIKEN Center for Developmental
Biology, Chuo, Kobe, Japan
Jorge o. Múnera • Division of Developmental Biology, Cincinnati Children’s Hospital,
Cincinnati, OH, USA
toKusHige naKano • Environmental Health Science Laboratory, Sumitomo Chemical Co .,
Ltd ., Osaka, Japan
Jing nie • Department of Otolaryngology—Head and Neck Surgery, and Stark
Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis,
IN, USA
ryuicHi nisHinaKaMura • Department of Kidney Development, Institute of Molecular
Embryology and Genetics, Kumamoto University, Kumamoto, Japan
taKa-aKi K. nogucHi • Graduate School of Life and Environmental Sciences,
The University of Tsukuba, Tsukuba, Ibaraki, Japan
MiHo ogawa • Laboratory for Organ Regeneration, RIKEN Center for Developmental
Biology, Kobe, Hyogo, Japan; Organ Technologies Inc ., Tokyo, Japan
MasaMitsu osHiMa • Department of Oral Rehabilitation and Regenerative Medicine,
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama
University, Okayama, Japan; Laboratory for Organ Regeneration, RIKEN Center for
Developmental Biology, Kobe, Hyogo, Japan
cHiKaFuMi ozone • Department of Endocrinology and Diabetes, Graduate School of
Medicine, Nagoya University, Nagoya, Aichi, Japan; Laboratory for Organ
Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan
Holly M. poling • Department of Pediatric General and Thoracic Surgery, Cincinnati
Children’s Hospital Medical Center, Cincinnati, OH, USA
adrian ranga • Laboratory of Stem Cell Bioengineering, Institute of Bioengineering,
School of Life Sciences and School of Engineering, Ecole Polytechnique Fédérale de
Lausanne (EPFL), Lausanne, Switzerland; Department of Mechanical Engineering,
KU Leuven, Belgium
Mírian roMitti • Institute of Interdisciplinary Research in Molecular Human Biology
(IRIBHM), Université Libre de Bruxelles, Brussels, Belgium
Hideya saKagucHi • In Vitro Histogenesis team, RIKEN Center for Developmental
Biology, Kobe, Hyogo, Japan; Department of Clinical Application, Center for iPS Cell
Research and Application (CiRA), Kyoto University, Kyoto, Japan
andrea alex scHiavo • Institute of Interdisciplinary Research in Molecular Human
Biology (IRIBHM), Université Libre de Bruxelles, Brussels, Belgium
KeisuKe seKine • Department of Regenerative Medicine, Yokohama City University
Graduate School of Medicine, Yokohama, Kanagawa, Japan
HidetaKa suga • Department of Endocrinology and Diabetes, Nagoya University Hospital,
Nagoya, Aichi, Japan
atsuHiro tagucHi • Department of Kidney Development, Institute of Molecular
Embryology and Genetics, Kumamoto University, Kumamoto, Japan