Optical probes in biology PDF
Preview Optical probes in biology
BIOLOGICAL SCIENCES/BIOPHYSICS Zhang SERIES IN CELLULAR AND CLINICAL IMAGING Ammasi Periasamy, series editor Mehta Optical Probes in Biology explores the diverse capabilities of these powerful Schultz and versatile tools and presents various approaches used to design, develop, and implement them. Top international scientists show you how to incorporate Optical Probes state-of-the-art optical probes and fluorescence imaging into your research. The book examines the use of optical probes to detect and track numerous molecular processes in living cells, including GTPase and kinase activities, membrane lipids, voltage, metal ions, metabolic signals, RNA, and histone in Biology modifications. It critically reviews the different probe designs and delves into the strategies for developing new fluorescent protein varieties with enhanced capabilities. It also covers sophisticated imaging techniques and equipment, such as both intensity and lifetime-based fluorescence microscopy methods, used to visualize and track optical probes. In addition, the book goes beyond live-cell tracking to discuss the growing application of activity-based probes for performing pharmacological drug screening and probing molecular processes in living animals. It also discusses emerging techniques that are expanding optical probe-based approaches into new biological frontiers. FEATURES • Focuses on the use of optical probes to track events in single cells and living animals • Describes the properties of fluorescent proteins and their importance in the design of fluorescent biosensors • Explains how Förster/fluorescence resonance energy transfer-based biosensors report key molecular information • Helps you choose the best probe deszgn for your application • Provides detailed discussions on selecting the appropriate imaging Edited by experiment and experimental set-up for answering a given biological Jin Zhang question • Covers several promising new techniques, including super-resolution microscopy that can observe biological phenomena in unprecedented Sohum Mehta spatial detail as well as optogenetic techniques that can both detect and manipulate intracellular events Carsten Schultz K14759 ISBN: 978-1-4665-1011-1 90000 9 781466 510111 K14759_Cover_PubGr.indd All Pages 12/9/14 1:20 PM Optical Probes in Biology Series in Cellular and Clinical Imaging Series Editor Ammasi Periasamy PUBLISHED Coherent Raman Scattering Microscopy edited by Ji-Xin Cheng and Xiaoliang Sunney Xie Imaging in Cellular and Tissue Engineering edited by Hanry Yu and Nur Aida Abdul Rahim Second Harmonic Generation Imaging edited by Francesco S. Pavone and Paul J. Campagnola The Fluorescent Protein Revolution edited by Richard N. Day and Michael W. Davidson Natural Biomarkers for Cellular Metabolism: Biology, Techniques, and Applications edited by Vladimir V. Gukasyan and Ahmed A. Heikal Optical Probes in Biology edited by Jin Zhang, Sohum Mehta, and Carsten Schultz SERIES IN CELLULAR AND CLINICAL IMAGING Ammasi Periasamy, series editor Optical Probes in Biology Edited by Jin Zhang Sohum Mehta Carsten Schultz CRC Press Taylor & Francis Group 6000 Broken Sound Parkway NW, Suite 300 Boca Raton, FL 33487-2742 © 2015 by Taylor & Francis Group, LLC CRC Press is an imprint of Taylor & Francis Group, an Informa business No claim to original U.S. Government works Version Date: 20140723 International Standard Book Number-13: 978-1-4665-1012-8 (eBook - PDF) This book contains information obtained from authentic and highly regarded sources. Reasonable efforts have been made to publish reliable data and information, but the author and publisher cannot assume responsibility for the validity of all materials or the consequences of their use. The authors and publishers have attempted to trace the copyright holders of all material reproduced in this publication and apologize to copyright holders if permission to publish in this form has not been obtained. If any copyright material has not been acknowledged please write and let us know so we may rectify in any future reprint. Except as permitted under U.S. Copyright Law, no part of this book may be reprinted, reproduced, transmit- ted, or utilized in any form by any electronic, mechanical, or other means, now known or hereafter invented, including photocopying, microfilming, and recording, or in any information storage or retrieval system, without written permission from the publishers. 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Visit the Taylor & Francis Web site at http://www.taylorandfrancis.com and the CRC Press Web site at http://www.crcpress.com Contents Series Preface ix Preface xiii Editors xvii Contributors xix SeCtion i introduction and Basics 1 Engineering of Optimized Fluorescent Proteins: An Overview from a Cyan and FRET Perspective 3 Lindsay Haarbosch, Joachim Goedhart, Mark A. Hink, Laura van Weeren, Daphne S. Bindels, and Theodorus W. J. Gadella 2 Fluorescent Imaging Techniques: FRET and Complementary Methods 33 Stefan Terjung and Yury Belyaev SeCtion ii tracking: Sensors for tracking Biomolecules 3 Protein-Based Calcium Sensors 73 Thomas Thestrup and Oliver Griesbeck 4 Monitoring Membrane Lipids with Protein Domains Expressed in Living Cells 89 Peter Varnai and Tamas Balla v vi Contents 5 Biosensors of Small GTPase Proteins for Use in Living Cells and Animals 137 Ellen C. O’Shaughnessy, Jason J. Yi, and Klaus M. Hahn 6 Molecular Beacon–Type RNA Imaging 167 Felix Hövelmann and Oliver Seitz 7 Optical Probes for Metabolic Signals 199 Yin Pun Hung and Gary Yellen 8 Genetically Encoded Voltage Indicators 229 Hiroki Mutoh, Walther Akemann, and Thomas Knöpfel 9 Prototypical Kinase Sensor Design Motifs for In Vitro and In Vivo Imaging 247 Gary C. H. Mo, Ambhighainath Ganesan, and Jin Zhang 10 RNA 267 Samie R. Jaffrey 11 Fluorescent Sensors for Imaging Zinc Dynamics in Biological Fluids 291 Wen-hong Li 12 Histone Modification Sensors in Living Cells 317 Hiroshi Kimura and Yuko Sato SeCtion iii Beyond Live-Cell tracking: Sensors for Diverse Applications 13 Chemical Probes for Fluorescence Imaging in Living Mice 335 Toshiyuki Kowada, Hiroki Maeda, and Kazuya Kikuchi Contents vii 14 Optical Probes for In Vivo Brain Imaging 355 Ksenia V. Kastanenka, Michal Arbel-Ornath, Eloise Hudry, Elena Galea, Hong Xie, and Brian J. Bacskai 15 Smart Imaging Probes for the Study of Protease Function 377 Ehud Segal and Matthew Bogyo SeCtion iV emerging techniques 16 Super-Resolution Imaging 413 Robert K. Neely, Wim Vandenberg, and Peter Dedecker 17 Imaging in Optogenetics 435 Xiaobo Wang, Li He, Carol A. Vandenberg, and Denise Montell
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