Nucleotide sequences coding for cis-aconitic decarboxylase and

40 Pages·2013·3.36 MB·English

US 20110099670A1
(19) United States
(12) Patent Application Publication (10) Pub. No.: US 2011/0099670 A1
Koops et al. (43) Pub. Date: Apr. 28, 2011
(54) NUCLEOTIDE SEQUENCES CODING FOR (30) Foreign Application Priority Data
CIS-ACONITIC DECARBOXYLASE AND USE
THEREOF Feb. 14; 2008 (EP) ................................ .. 081514523
Publication Classi?cation
(76) Inventors: Andries Jurriaan Koops; (51) Int. Cl.
Opheusden (NL); Leendert C121’ 7/44 (200601)
Hendrik De Graaff; Arnhem (NL); CIZN 9/88 (200601)
Ingrid Maria Van Der Meer; C12N 15/00 (200601)
Wagemn. gen (N L);_ W1l. helmus C12N 5/10 (2006.01)
, , C12N 1/15 (2006.01)
Antonius Marla Van Den Berg, C1 2N 1/19 (200601)
Renkum (NL) A01H 5/00 (2006.01)
C07C 57/13 (2006.01)
. (52) US. Cl. ................... .. 800/298; 536/232; 435/320.1;
(21) Appl' No" 12/867’030 435/419; 435/254.11; 435/2543; 435/2545;
435/254.22; 435/142; 562/590
(22) PCT Filed: Feb. 13, 2009 (57) ABSTRACT
The present invention relates to nucleotide sequences encod
(86) PCT No.: PCT/NL09/50065 ing polypeptides With cis-aconitic decarboxylase activity; the
cells transformed With such nucleotide sequences; preferably
§ 371 (c)(1); fungal or plant cells; and to methods Wherein such trans
(2); (4) Date: Dec. 13, 2010 formed cells are use for the production of itaconic acid.
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