Nucleic Acid Transfection

316 Pages·2010·9.133 MB·English

by  Peter Hahn, Elizabeth Scanlan (auth.), Wolfgang Bielke, Christoph Erbacher (eds.)

296
Topics in Current Chemistry
Editorial Board:
A. de Meijere K.N. Houk C.A. Hunter H. Kessler
l l l
J.-M. Lehn S.V. Ley M. Olivucci J. Thiem B.M. Trost
l l l l
M. Venturi P. Vogel H. Wong H. Yamamoto
l l l
Topics in Current Chemistry
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Nucleic Acid Transfection
Volume Editors:
(cid:1)
Wolfgang Bielke Christoph Erbacher
With Contributions by
A. Ahmad (cid:1) M.N. Bacon (cid:1) K. Berg (cid:1) M. Berstad (cid:1) N.F. Bouxsein (cid:1)
C. Bra¨uchle (cid:1) M. Bradley (cid:1) M. Caldero´n (cid:1) H.M. Evans (cid:1) K.K. Ewert (cid:1)
W.Fischer(cid:1) R.Haag(cid:1) P.Hahn(cid:1) I.Hedfors(cid:1) A.Høgset(cid:1) R.Koynova(cid:1)
C.S. McAllister (cid:1) P.M. McLendon (cid:1) L. Prasmickaite (cid:1) T.M. Reineke (cid:1)
N.Ruthardt (cid:1) C.R.Safinya (cid:1)E.E.Salcher (cid:1)C.E. Samuel(cid:1) E.Scanlan (cid:1)
P.K. Selbo (cid:1) A. Sizovs (cid:1) S. Srinivasachari (cid:1) B. Tenchov (cid:1)
A. Unciti-Broceta (cid:1) E. Wagner (cid:1) A. Weyergang (cid:1) A. Zidovska
Editors
Dr.WolfgangBielke Dr.ChristophErbacher
QIAGENGmbH QIAGENGmbH
QiagenStr.1 DivisionCorporateResearch
40724Hilden QiagenStr.1
Germany 40724Hilden
[email protected] Germany
[email protected]
ISSN0340-1022 e-ISSN1436-5049
ISBN978-3-642-16429-3 e-ISBN978-3-642-16430-9
DOI10.1007/978-3-642-16430-9
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QiagenStr.1 DivisionCorporateResearch
40724Hilden QiagenStr.1
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[email protected] Germany
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Editorial Board
Prof.Dr.ArmindeMeijere Prof.Dr.Jean-MarieLehn
Institutfu¨rOrganischeChemie ISIS
derGeorg-August-Universita¨t 8,alle´eGaspardMonge
Tammanstr.2 BP70028
37077Go¨ttingen,Germany 67083StrasbourgCedex,France
[email protected] [email protected]
Prof.Dr.KendallN.Houk Prof.Dr.StevenV.Ley
UniversityofCalifornia UniversityChemicalLaboratory
DepartmentofChemistryandBiochemistry LensfieldRoad
405HilgardAvenue CambridgeCB21EW
LosAngeles,CA90024-1589,USA GreatBritain
[email protected] [email protected]
Prof.Dr.ChristopherA.Hunter Prof.Dr.MassimoOlivucci
DepartmentofChemistry Universita`diSiena
UniversityofSheffield DipartimentodiChimica
SheffieldS37HF,UnitedKingdom ViaADeGasperi2
c.hunter@sheffield.ac.uk 53100Siena,Italy
[email protected]
Prof.Dr.HorstKessler
Prof.Dr.JoachimThiem
Institutfu¨rOrganischeChemie
TUMu¨nchen Institutfu¨rOrganischeChemie
Lichtenbergstraße4 Universita¨tHamburg
86747Garching,Germany Martin-Luther-King-Platz6
[email protected] 20146Hamburg,Germany
[email protected]
vi EditorialBoard
Prof.Dr.BarryM.Trost Prof.Dr.HenryWong
DepartmentofChemistry TheChineseUniversityofHongKong
StanfordUniversity UniversityScienceCentre
Stanford,CA94305-5080,USA DepartmentofChemistry
[email protected] Shatin,NewTerritories
[email protected]
Prof.Dr.MargheritaVenturi
Prof.Dr.HisashiYamamoto
DipartimentodiChimica
Universita`diBologna ArthurHollyCompton
viaSelmi2 DistinguishedProfessor
40126Bologna,Italy DepartmentofChemistry
[email protected] TheUniversityofChicago
5735SouthEllisAvenue
Prof.Dr.PierreVogel Chicago,IL60637
773-702-5059
LaboratoryofGlycochemistry
USA
andAsymmetricSynthesis
[email protected]
EPFL–Ecolepolytechniquefe´derale
deLausanne
EPFLSBISICLGSA
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1015Lausanne,Switzerland
pierre.vogel@epfl.ch
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Preface
Transfectionofmacromoleculesintovariouscelltypesortissues,eitherinvitroor
in vivo, has become an indispensable technique for most fields of biomedical
research. Furthermore, future applications will likely lead to an increase in the
importance of transfection technologies since, with the exception of using certain
drugs, there is currentlylittle alternative to transfection for gene-specific manipu-
lationofcells.Typically,transfectionsareperformedwithvarioustypesofnucleic
acids such as genetic expression constructs, siRNAs, and occasionally messenger
RNAs. Although many physical and viral transfection methods have been devel-
oped for use in different applications, the majority of protocols use chemical
transfectionreagents.
In this volume, we have brought together articles from various experts in
thechemicaltransfectionreagentfield.Topicscoveredincludedescriptionsofthe
chemistry of chemical transfection compounds and details of the fundamental
parametersthatinfluencetransfectionefficiencies.
ThearticlebyHahnandScanlangivesageneraloverviewofcommontransfec-
tionmethodsandpresentsavaluableentrancepointtothefield.
Students,butalsoadvancedresearchers,areadvisedtocontinuewiththearticle
by Unciti-Broceta et al., which gives a comprehensive outline of the chemistry
underlyingliquidandsolidsynthesisofcationiclipidsanddendrimers.
Asummary anddiscussion ofempirical data on relationships between cationic
lipid molecular structure and transfection activity are provided by Koynova and
Tenchov Structural features of cationic lipids are related to their interaction with
biomembranesandtheuptakeefficiencyoftransfectioncomplexes.
Several authors describe distinct classes of chemical transfection reagents.
Fischer et al. summarize synthesis methods, features, and applications of hyper-
branchedpolyaminesascarriersfornucleicacids.
A review from Sizovs et al. discusses carbohydrate-based polymeric transfec-
tionreagents,turningspecialattentiontotheirpotentialapplicationsinpreclinical
modelsandforthetreatmentofdiseases.
Ewert et al. present recent work on structural studies of cationic lipid/nucleic
acid interaction, optimized for therapeutic nucleic acid delivery. Fundamental
ix