Novel Approaches in Biosensors and Rapid Diagnostic Assays: 43rd OHOLO Conference Eilat, Israel, October 10–14, 1999

321 Pages·2001·15.018 MB·English

by  Frank J. Steemers, Jane A. Ferguson (auth.), Zvi Liron, Avraham Bromberg, Morly Fisher (eds.)

Novel Approaches in
Biosensors and Rapid
Diagnostic Assays
43rd OHOLO Conference
Eilat, Israel, October 10-14, 1999
NOVEL APPROACHES IN BIOSENSORS
AND RAPID DIAGNOSTIC ASSAYS
Israel Institute for Biological Research
Ness-Ziona, Israel
US-Israel Science and Technology Commission
Scientific Organizing Committee
Zvi Liron, Avraham Bromberg,
Morly Fisher, Yoram Yahav
Scientific Advisory Committee
D. Walt, H. Vogel, I. Willner, S. Marx,
F. Fish, D. Katz
Permanent Organizing Committee
A. Shafferman, A. Golombek, S. Kinamon
Novel Approaches in
Biosensors and Rapid
Diagnostic Assays
Edited by
Zvi Liron
Avraham Bromberg and
Morly Fisher
Israel Institute for Biological Research
Ness-Ziona, Israel
Springer Science+Business Media, LLC
Library of Congress Cataloging-in-Publication Data
Novel approaches in biosensors and rapid diagnostic assays/edited by Zvi Liron,
Avraham Bromberg, and Morly Fisher,
p. ; cm.
Includes bibliographical references and index.
ISBN 978-1-4613-5452-9 ISBN 978-1-4615-1231-8 (eBook)
DOI 10.1007/978-1-4615-1231-8
1. Biosensors—Congresses. I. Liron, Zvi. II. Bromberg, Avraham. III. Fisher, Morly.
IV. OHOLO Conference (43rd: 1999: Eilat, Israel)
[DNLM: 1. Biosensing Techniques—Congresses. 2. Biological Assay—Congresses. QT
36 N937 2000]
R857.B54 N685 2000
610'.28—dc21
00-046608
Proceedings of the 43rd OHOLO Conference on Novel Approaches in Biosensors and Rapid
Diagnostic Assays, held October 10-14, 1999, in Eilat, Israel
ISBN 978-1-4613-5452-9
©2001 Springer Science+Business Media New York
Originally published by Kluwer Academic/Plenum Publishers, New York in 2001
Softcover reprint of the hardcover 1 st edition 2001
http://www.wkap.nl/
10 9 8 7 6 5 4 3 21
A CLP. record for this book is available from the Library of Congress
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted ni any form or by
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permission from the Publisher
PREFACE
In the medical, food, and environmental fields there is a continuous demand for
inexpensive and sensitive analytical devices that are reliable, rapid, capable of high
throughput screening, and have low cost per test unit. Small and portable biosensor
devices are designed to fulfill most of these requirements, and can be used in laboratory
and on-site field testing.
The multidisciplinary nature of biosensors research was illuminated by the topics
presented in the 43rd OHOLO conference on "Novel Aproaches in Biosensors and
Rapid Diagnostic Assays" held in Eilat, Israel in October 1999.
This conference proceedings volume discussed major issues in optical, acoustic
and electrochemical-based biosensors, biochips, sensing recognition elements, and
biosensors for medical and environmental applications. The papers presented at the
conference represent basic and applied research studies in the fields of diagnostic
assays and biosensor development. Novel technologies, such as arrays of sensors using
high-density fiber optics to sense labeled or unlabeled oligonucleotides, and patterned
arrays of recognition elements, demonstrated the capability of biosensors to analyse
multiple analytes.
Optical techniques, such as thin film metal oxide waveguides, have been applied
for sensor development. Acoustic wave devices were used in the study of biochemical
macromolecules and cells immobilized within the sensor-liquid interface, and were
also utilized to monitor antigen-antibody and ligand-receptor interactions. Electro
chemical techniques have been utilized to monitor DNA hybridization in real time, and
in the development of screen-printed electrodes based on enzyme amplification. These
techniques have been used also in development of new DNA sensors and immunosen
sors based on biocatalyzed precipitation of insoluble products, and in the development
of new bioelectrodes based on noble metal nanoparticles composite sol-gel instead of
the traditional carbon particles.
The potential of synthetic receptors prepared form imprinted polymers as recog
nition elements was demonstrated by the binding of caffeine to the tailored cavity
formed in the presence of caffeine and monitored by an acoustic sensor. Optimized
polymer imprinting techniques have been achieved by using nano-imprinted particles,
composite imprints, and surface impriting. Bio-recognition elements such as the
v
vi PREFACE
avidin-biotin system or cell membrane receptors were demonstrated for sensing and
for signal transduction.
The new biochip technology enabled comprehensive studies of neuronal nicotinic
acetylcholine receptor response in physiological conditions. Standardization of these
techniques is necessary in DNA analysis. Polymer materials have been utilized in
development of a new inexpensive disposable sensor based on reflection holograms, in
colorimetric sensor based on self-assembled polymers, and in the development of new
latex microspheres widely used in medical diagnostic applications. Microsphere utili
zation was also demonstrated in the luminescence oxygen channeling assay.
Overviews of past and present rapid diagnostic methods used in microbiology as
well as new advanced technologies for detection of biological materials in the natural
environment were presented. Practical medical applications have been demonstrated in
studies of continuous and direct in situ measurements of temporal variations in the
oxygen partial pressure during photodynamic therapy, and in the study of glucose
monitoring by combined iontophoretic extraction with an in situ glucose sensor.
Last but not least, the interaction among participants from different countries and
of different expertise and the informal discussions were vital to the success of the
conference.
We are grateful to all contributors to this book and special thanks should be
expressed to our colleagues: D. Walt, H. Vogel, I. Willner, S. Marx, A. Raz and N.
Kaushansky.
ACKNOWLEDGMENTS
The Organizing Committee gratefully acknowledges the generous financial contribu
tion of the following organizations:
U.S.-Israel Science & Technology Commission, Tel-Aviv, Israel
Ministry of Tourism, Jerusalem, Israel
vii
CONTENTS
1. Analyzing Unamplified Gene Sequences with High Density Optical
Fiber Arrays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Frank J. Steemers, Jane A. Ferguson, and David R. Walt
2. Array Biosensor for Simultaneous Detection of Multiple Analytes 7
Fraces S. Ligler, James W. Hazzard, Joel P. Golden, and Chris A. Rowe
3. Acoustic Wave Study of Interfacially-Bound Proteins, Nucleic Acids
and Blood Platelets ........................................... 17
M. Thompson, B. A. Cavic, L. M. Furtado, Z. Morel, and N. Tassew
4. High Frequency Acoustic STW Biosensor ........................ 33
Zvi Liron, Nathali Kaushansky, Netzach Graziani, Avi Keysary,
Itzhak Barness, and Sharon Marx
5. Ultrasensitive and Specific Electronic Transduction of DNA Sensing
Processes ................................................... 47
Itamar Willner, Fernando Patolsky, Lital Alfonta, Amir Lichtenstein,
and Bilha Willner
6. Enzyme-Amplified Amperometric Detection of Hybridization and
of a Single Base Pair Mutation in an 18 Base Oligonucleotide
on a 7 IJ..m Diameter Microelectrode ............................. 79
Daren J. Caruana, Thierry de Lumley-Woodyear, and Adam Heller
7. Development and Application of Bioelectrochemical Sensors for
On-Site Monitoring ........................................... 101
l. Eshkenazi, T. Neufeld, V. Sacks, Y. Herschkovitz and J. Rishpon
8. Sol-Gel Derived Composite Ceramic Carbon Biosensors 111
L. Rabinovich and o. Lev
ix
x CONTENTS
9. Holographic Biosensors ....................................... 127
Christopher R. Lowe, Andrew G. Mayers, Jeff Blyth,
and Roger B. Millington
10. Waveguide Fluorosensor for the Detection of Ligand-Receptor
Interactions: The 5-HT 3 Serotonin Receptor ....................... 135
Evelyne L. Schmid, Ruud Hovius, Michael Pawlak, Markus Ehrat,
and Horst Vogel
11. Importance of Biochips in the Study of Neuronal Nicotinic
Acetylcholine Receptors: Biochips and Nicotinic Receptors .......... 147
Y. Villiger, M. O. Heusckel, J. Romand, K. H. Karuse, P. Renaud,
and D. Bertrand
12. Improving DNA-Chip Technology: Chemical Aspects. . . . . . . . . . . . . . . 165
Jorg D. Hoheisel, Frank Diehl, Marcel Scheideler, Nicole Hauser,
Verena Aign, Stefan Matysiak, and Markus Beier
13. The Application of Biorecognition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173
Meir Wilchek, Heike Hofstetter, and Oliver Hofstetter
14. The Use of Imprinted Polymers as Recognition Elements in Biosensors
and Binding Assays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 193
Ecevit Yilmaz, Lei Ye, Karsten Haupt, and Klaus Mosbach
15. Molecular Imprinting and Chemical Sensing: Synthetic Antibodies 211
F. L. Dickert, K. Halikias, O. Hayden, P. Liu, and A. Rohrer
16. Electropolymerizable Bipyrrole-Biotin: A New Derivative for the
Immobilization of Ligands onto Electrode Surfaces ................. 225
Karine Perie, Vitali Strokhin, Robert S. Marks,
and Jean-Paul Lellouche
17. New Approaches to Rapid Diagnostics Using Bichromic Polymers and
Organic Thin Films . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 235
Deborah H. Charych
18. Recent Uses of Microspheres in Diagnostic Tests and Assays. . . . . . . . . 245
Leigh B. Bangs
19. Luminescence Oxygen Channeling Assay (LOCI~): A Highly Sensitive
and Versatile Homogeneous Assay Method . . . . . . . . . . . . . . . .. . . . . . . . 265
Nurith Kum
CONTENTS xi
20. Direct and Continuous Measurements of Oxygen Partial Pressure Using
a Tissue-Inserted Optical Oxygen Microsensor: During Photodynamic
Therapy .................................................... 273
1. Zilberstein, Y. Salomon, A. Scherz, and A. Bromberg
21. Glucose Monitoring by Reverse Iontophoresis 285
Michael 1. Tierney
22. Rapid Methods of Microbiological Analysis: An Update . . . . . . . . . . . .. 295
Daniel Y. C. Fung
23. Revolutionary Technologies for Bio Detection 313
Midred A. Donlon
Index...... . .... ....... . .......... . . . .... . .. ...... . . . ........ .. 319