Nitric Oxide Part B: Physiological and Pathological Processes

570 Pages·1996·10.315 MB·English

by  Lester Packer (Eds.)

Methods in Enzymology
Volume 269
Nitric Oxide
Part B
Physiological and
Pathological Processes
EDITED BY
Lester Packer
DEPARTMENT OF MOLECULAR AND CELL BIOLOGY
UNIVERSITY OF CALIFORNIA
BERKELEY. CALWORNIA
Editorial Advisory Board
Bruce N. Ames
Joseph Beckman
Enrique Cadenas
Victor Darley-Usmar
Bruce Freeman
Barry Halliwell
Louis J. Ignarro
Hlroe Nakazawa
William Pryor
Helmut Sits
kp
0
ACADEMIC PRESS
San Diego New York Boston London Sydney Tokyo Toronto
Contributors to Volume 269
elcitrA numbers are in parentheses gniwollof the names of .srotubirtnoc
snoitailiffA listed are .tnerruc
BHARAT B. AGGARWAL (15), Cytokine Re- BERNHARD BRONE (38), Department of IV~
search Laboratory, Department of Molecu- Experimental Division, Faculty of Medi-
lar Oncology, M. D. Anderson Cancer Cen- cine, University of Erlangen-Niinberg,
ter, University of Texas, Houston, Texas 45019 Erlangen, Germany
03077 A. BUSCH (44), Pediatric Respiratory Depart-
BRUCE N. AMES (24), Division of Biochemis- ment, Royal Brompton Hospital, London
try and Molecular Biology, University of SW3 6NP, United Kingdom
California, Berkeley, California 02749
THILO BUSCH (42), Klinik far Anaesthesiolo-
ERNST V. ARNOLD (5), Department of Chem-
gie und Operative Intensivmedizin, Vir-
istry, University of Wyoming, Laramie, Wy-
chow-Klinikum, Humboldt Universiti~t
oming 17028
Berlin, 13353 Berlin, Germany
OHARA AUGUSTO (32), Department of Bio-
C. A. BYRNES (44), Pediatric Respiratory De-
chemistry, Instituto de Quimica, Universi-
partment, Royal Brompton Hospital, Lon-
dade de Sao Paulo, 05599 Sao Paulo, Brazil
don SW3 6NP, United Kingdom
ETELVINO J. H. BECHARA (37), Instituto de
Quimica, Universidade de Sao Paulo, 80550 ENRIQUE CADENAS (7), Department of Molec-
Sao Paulo, Brazil ular Pharmacology and Toxicology, School
JOSEPH S. BECKMAN (19, 20, 27, 31), Depart- of Pharmacy, University of Southern Cali-
ments of Anesthesiology and Biochemistry, fornia, Los Angeles, California 33009
University of Alabama, Birmingham, Ala- MARIA C. CARRERAS (7), Laboratory of Oxy-
bama 33253 gen Metabolism, Margarita Companc de P.
NANCY BOERTH (14), Division of Molecular Acuaa, Department of Clinical Biochemis-
and Cellular Pathology, Department of try, University Hospital, University of Bue-
Pathology, University of Alabama, Bir- nos Aires, 1113 Buenos Aires, Argentina
mingham, Alabama 35294 STEPHEN CHRISTEN (24), Division of Bio-
D. ScoTt BOHLE (5, 28), Department of chemistry and Molecular Biology, Univer-
Chemistry, University of Wyoming, Lara- sity of California, Berkeley, California
mie, Wyoming 17028 02749
ALBERTO BOVERIS (7), Institute of Physical TRUDY L. LLEWNROC (14), Division of Molec-
Chemistry, School of Pharmacy and Bio-
ular and Cellular Pathology, Department of
chemistry, University of Buenos Aires, 3111
Pathology, University of Alabama, Bir-
Buenos Aires, Argentina
mingham, Alabama 35294
DAVID S. BREDT (11), Department of Physiol-
CARROLL E. CROSS (16), Department oflnter-
ogy and Program in Biomedical Sciences,
nil Medicine, Division of -itirC~yranomluP
School of Medicine, University of Califor-
cal Care Medicine, University of ,ainrofilaC
nia, San Francisco, California 34149
Davis, California 61659
JAY E. BRENMAN (11), Department of Physiol-
ogy and Program in Biomedical Sciences, JOHN P. CROW (17), Department of Anesthesi-
School of Medicine, University of Califor- ology, University of Alabama, Birmingham,
nia, San Francisco, California 34149 Alabama 35294
KARLIS BRIVIBA (37), Institute far Physiolo- RAFAEL CUETO (26, 29), Biodynamics Insti-
gische Chemie I, Heinrich-Heine-Universi- tute, Louisiana State University, Baton
tat, D-40001 Diisseldorf Germany Rouge, Louisiana 30807
ix
X CONTRIBUTORS TO VOLUME 269
VICTOR M. DARLEY-USMAR (35), Department PETER A. GLASSBRENNER (28), Department
of Pathology, Division of Molecular and of Chemistry, University of Wyoming, Lara-
Cellular Pathology, University of Alabama, mie, Wyoming 17028
Birmingham, Alabama 49253 D. NElL GRANGER (41), Department of Physi-
CAROL A. DELANEY (23), Biochemistry Labo- ology, Louisiana State University Medical
ratory, School of Biological Sciences, Sus- Center, Shreveport, Louisiana 03117
sex University, Falmer, Brighton, East Sus- IRENE C. GREEN (23), Biochemistry Labora-
sex BN1 9QG, United Kingdom tory, School of Biological Sciences, Sussex
TERESA DEROJAS-WALKER (22), Division of University, Falmer, Brighton, East Sussex
Toxicology, Massachusetts Institute of BN1 9QG, United Kingdom
Technology, Cambridge, Massachusetts
MICHAEL H. L. GREEN (23), MRC Cell Muta-
93120
tion Unit, Sussex University, Falmer, Brigh-
PAOLO DI MASCIO (37), Instituto de Quimica, ton, East Sussex BN1 9RR, United Kingdom
Universidade de Sao Paulo, 05508 Sao
BARRY HALLlWELL (16), Neurodegenerative
Paulo, Brazil
Diseases Research Center, King's College,
JEAN-CLAUDE DRAPIER (3), Section de Biolo- Chelsea Campus, University of London,
gic, Institute Curie, Unitd 563 INSERM, London SW3 6LX, United Kingdom
13257 Paris Cedex, France
TRACY P. HAMILTON (31), Department of
JASON P. EISERICH (16), Department of Inter- Chemistry, University of Alabama, Bir-
nal Medicine, Division of Pulmonary/Criti-
mingham, Alabama 35294
cal Care Medicine, University of ,ainrofilaC
BERNHARD HANSERT (28), Department of
Davis, California 61659
Chemistry, University of Wyoming, Lara-
REHPOTSIRHC H. EVANS (8), Departments of
mie, Wyoming 17028
Orthopaedic Surgery and Molecular Genet-
ics and Biochemistry, School of Medicine, ALFRED HAUSLADEN (4), Department of Bio-
chemistry, Duke University Medical ,retneC
University of Pittsburgh, Pittsburgh, Penn-
Durham, North Carolina 27710
sylvania 16251
KONRAD FALKE (42), Klinik far Anaesthesi- JOHN B. HIBBS, JR. (3), Division of Infectious
ologie und Operative Intensivmedizin, Vir- Disease, Department of Medicine, Univer-
chow-Klinikum, Humboldt t~itisrevinU Ber- sity of Utah, and Department of Veterans
lin, 13353 Berlin, Germany Affairs Medical Center, Salt Lake City,
Utah 84148
K. FEHSEL (40), Research Group lmmunobi-
ology 14.80, MED-Heinrich-Heine Uni- ADRIAN J. HOBBS (13), Department of Phar-
versitat, D-40225 Dusseldorf, Germany macology, School of Medicine, University
of California, Los Angeles, California
BRUCE A. FREEMAN (36), Department of An-
59009
esthesiology, Biochemistry and Molecular
Genetics, University of Alabama, Bir- ZHI-QIANG HUANG (19), Department of Neu-
mingham, Alabama 33253 rology, University of Western Ontario, Lon-
IRWIN FRIDOVICH (4), Department of Bio- don, Ontario N6A 3K7, Canada
chemistry, Duke University Medical ,retneC ONITNATSOC IADECOLA (39), Department of
Durham, North Carolina 27710 Neurology, University of Minnesota Medi-
JOHN GARTHWAITE (12), The Cruciform Proj- cal School, Minneapolis, Minnesota 55455
ect, London, W1P 9LN, United Kingdom Louis J. IGr~ARRO (13), Department of Phar-
REYNALDO M. GATTI (32), Department of macology, School of Medicine, University
Biochemistry, Instituto de Quimica, Uni- of California, Los Angeles, California
versidade de Sao Paulo, 05599 Sao Paulo, 59009
Brazil MASAYASU INOUE (45), Department of Bio-
PAULINE GEE (24), Xenometrix, Inc., Boulder, chemistry, Osaka City University Medical
Colorado 10308 School, Osaka 545, Japan
CONTRIBUTORS TO VOLUME 269 xi
YRRAH SOLUOPORIHCSI (17), Institute for En- NAILLIJ E. LOWE (23), MRC Cell Mutation
vironmental Medicine, University of Penn- Unit, Sussex University, Falmer, Brighton,
sylvania, Philadelphia, Pennsylvania 40191 East Sussex BN1 9RR, United Kingdom
XIA JIN (34), Biodynamics Institute, Louisiana EMIAJ T. RERREFSAM (2), Inflammatory Dis-
State University, Baton Rouge, Louisiana eases Research, .G D. Searle, .tS Louis, Mis-
30807 souri 63017
HPESOJ RENNAK (21), Department of Food ASIRAM n. G. SORIEDEM (37), Instituto de
Science, Agriculture Research Organiza- Quimica, Universidade de DaS Paulo, 80550
tion, The Volcani Center, Bet Dagan DaS Paulo, Brazil
,05205 Israel LIPAK ATHEM (15), Department of Bioimmu-
HSAKRAPRAH KAUR (16), Neurodegenerative notherapy, hi. D. Anderson Cancer Center,
University of Texas, Houston, Texas 03077
Diseases Research Center, King's College,
Chelsea Campus, University of London, SAMOHT MICHEL (6), Cardiovascular Divi-
London SW3 6LX, United Kingdom sion, Brigham and Women's Hospital, Har-
vard Medical School, Boston, Massachu-
R. RENSSIK (27), Laboratorium far Anor-
setts 51120
ganische Chemie, EidgenOssische Tech-
nische Hochschule, CH-8092 Ziirich, Swit- OKIKUY AMAYIMANIM (45), Department of
zerland Biochemistry, Osaka City University Medi-
cal School, Osaka 545, Japan
V. NEFOHCAB-BLOK (25, 40), Research Group
Immunobiology 14.80, MED-Heinrich- .S AJAMDAP (18), Department of Chemistry,
Louisiana State University, Baton Rouge,
Heine-University, D-40001 Dusseldorf,
Louisiana 30807
Germany
HSEKAR P. LETAP (35), Department of Biolog-
INIMDAP SALIVALAMOK (14), Division of Mo-
ical and Chemical Sciences, University of
lecular and Cellular Pathology, Department
Essex, Colchester C04 3SQ, United
of Pathology, University of Alabama, Bir-
Kingdom
mingham, Alabama 35294
NAUJ J. OSOREDOP (7), Laboratory of Oxygen
MELLIW H. LONEPPOK (18, 27), Laboratorium
Metabolism, Margarita Companc de .P
far Anorganische Chemie, EidgenOssische
Acu~a, Department of Clinical Biochemis-
Technische Hochschule, CH-8092 Ziirich,
try, University Hospital, University of Bue-
Switzerland
nos Aires, 1113 Buenos Aires, Argentina
K.-D. EKCNORK (25, 40), Research Group Im-
MAILLIW A. ROYRP (26, 29, 30, 34), Biody-
munobiology ,08.41 MED-Heinrich-Heine-
namics Institute, Louisiana State University,
University, D-40001 Dusseldorf, Germany
Baton Rouge, Louisiana 30807
DIVAD D. Ku (10), Department of Pharmacol- LEAEAR RADI (32, 33), Department of Bio-
ogy and Toxicology, University of Ala- chemistry, Faculty of Medicine, Universidad
bama, Birmingham, Alabama 35294 de la Republica, 11800 Montevideo, -rU
PAUL SEBUK (41), Department of Physiology aguay
and Biophysics, Health Sciences Center, KERAM W. IKSMODAR (9), Departments of
The University of Calgary, Calgary, Alberta Obstetrics and Gynaecology and Pharma-
T2N 4N1, Canada cology, University of Alberta, Edmonton,
ODRAUDE G. ANITEPAL (38), Molecular Car- Alberta T6G 2S2, Canada
diovascular Research, Case Western Univer- M. .S NAINAZEMAR (18), Department of
sity School of Medicine, Cleveland, Ohio Chemistry, Louisiana State University, Ba-
60144 ton Rouge, Louisiana 30807
SAMOHT M. NLOCNIL (14), Division of Molec- LISA J. NOSNIBOR (6), Cardiovascular Divi-
ular and Cellular Pathology, Department of sion, Brigham and Women's Hospital, Har-
Pathology, University of Alabama, Bir- vard Medical School, Boston, Massachu-
mingham, Alabama 35294 setts 51120
xii CONTRIBUTORS TO VOLUME 269
YRNEH NESOR (20), Department of Medicine, TIANS TAMIR (22), Division of Toxicology,
University of Washington, Seattle, Washing- Massachusetts Institute of Technology,
ton 59189 Cambridge, Massachusetts 93120
M. HTEBAZILE Ross (39), Department of Neu- NEVETS R. MUABNENNAT (22), Division of
rology, University of Minnesota Medical Toxicology, Department of Chemistry,
School, Minneapolis, Minnesota 55455 Massachusetts Institute of Technology,
RoLE TNIASSOR (42), Klinik far Anaesthesi- Cambridge, Massachusetts 93120
olog& und Operative Intensivmedizin, Vir- EMUAJ SERROT (1), Department of Biological
chow-Klinikum, Humboldt Universitiit Ber- and Chemical Sciences, Central Campus,
lin, 13353 Berlin, Germany Wivenhoe Park, FOC 3SQ Colchester,
OREMOH RUBBO (36), Department of Bio- United Kingdom
chemistry, Faculty of Medicine, University
HuI-Hsu TSAI (31), Department of Chemistry,
of the Republic, Montevideo, CP ,00811 -rU
University of Alabama, Birmingham, Ala-
uguay
bama 35294
ODRAUDE SALAS (9), Departments of Obstet-
RAO M. UePu (26, 29, 30, 34), Biodynamics
rics and Gynaecology, University of Al-
Institute, Louisiana State University, Baton
,atreb Edmonton, Alberta T6G 2S2, Canada
Rouge, Louisiana 30807
ALEINAD INIMEVLAS (2), Inflammatory Dis-
KCIRTAP ECNALLAV (43), Department of
eases Research, .G D. Searle, .tS Louis, Mis-
Pharmacology, Clinical Pharmacology, .tS
souri 63017
George's Hospital Medical School, London
ADNICAJ B. NOSPMAS (20), Departments of SW17 ORE, United Kingdom
Anesthesiology and Biochemistry, Univer-
TREBLA NAV RED VLIET (16), Department of
sity of Alabama, Birmingham, Alabama
Internal Medicine, Division of Pulmonary/
33253
Critical Care Medicine, University of Cali-
E. A. ENRUOBENIHS (44), Pediatric Respira-
fornia, Davis, California 61659
tory Department, Royal Brompton Hospi-
tal, London SW3 6NP, United Kingdom ETTENNAEJ RAVIV-ZEUOSAV (32), Depart-
ment of Biochemistry, Instituto de Quimica,
TUMLEH SEIS (37), Institute far Physiologische
Universidade de Sao Paulo, 05599 S~o
Chemie I, Heinrich-Heine- ,tiitisrevinU
Paulo, Brazil
10004-D Diisseldorf Germany
NOMIS C. SNIKTAW (8), Departments of Cell
ERIC MAHTUOS (12), Glaxco Wellcome Medi-
Biology and Physiology, School of Medi-
cines Research Center, Stevenage, Herts.,
cine, University of Pittsburgh, Pittsburgh,
SG1 2NY, United Kingdom
Pennsylvania 16251
EPPESUIG L. OTIRDAUQS (26, 29, 34), Biody-
namics Institute, Louisiana State University, LEAHCIM T. NOSLIW (1), Department of Bio-
Baton Rouge, Louisiana 30807 logical and Chemical Sciences, Central
Campus, Wivenhoe Park, FOC 3SQ Col-
MAJA CI~AR-~(IVONAEETS (8), Departments of
chester, United Kingdom
Orthopaedic Surgery and Molecular Genet-
ics and Biochemistry, School of Medicine, NHOJ .S KONHSlW (22), Division of Toxicol-
University of Pittsburgh, Pittsburgh, Penn- ogy, Massachusetts Institute of Technology,
sylvania 16251 Cambridge, Massachusetts 93120
LEAHCIM GNORTS (19), Department of Medi- YAO ZU YE (19), Department of Anesthesiol-
cine, University of Alabama, Birmingham, ogy, University of Alabama, Birmingham,
Alabama 33253 Alabama 33253
UZAKEGIHS ARUMEKAT (45), Department of SAMAT ZAKAR (9), Departments of Obstetrics
Biochemistry, Osaka City University Medi- and Gynaecology, University of Alberta,
cal School, Osaka 545, Japan Edmonton, Alberta T6G 2S2, Canada
Preface
The realization that nitric oxide (NO.), a free radical that is generated
in biological systems, plays a pivotal role in physiology, pathology, and
pharmacology has led to an explosion of new research. The recognition
that the endothelial relaxing factor (ERDF) is actually NO. has been im-
portant in revolutionizing our thinking about how the vasculature is regu-
lated. The physiological functions of NO. in signaling by the activation of
guanylate cyclase and the existence of a multitude of other targets have
now been identified for NO- in biological systems.
NO. is a free radical species, and therefore reactive as such. However,
it reacts with the superoxide anion with a rate constant of about 6.7 x 901
M 1- sec 1 forming peroxynitrite (ONOO-), a species more reactive toward
lipids, DNA, and proteins, leading to their chemical modification and to
pathological effects.
Overcoming difficulties in accurately assessing its generation, detection,
and characterization in biological systems was the impetus for seeking
contributions to these two Methods in Enzymology volumes on nitric oxide
(268 and 269). They provide a comprehensive and detailed account of the
methodology relating to four areas. In this volume methods and assays
relevant to the effects of NO. in cells and tissues and the pathological and
clinical aspects of NO. are included. The articles in Section I focus on the
reactivity of NO. and tissue-specific effects of NO. as well as on its effects in
cell signaling mechanisms. The emphasis of Section II is on the pathological
action of NO. recognized by reactions of nitrosylation and nitration and
on the interaction of NO. with membranes, proteins, and nucleic acids.
Methods for detecting the action of peroxynitrite, tissue and cell toxicity,
and clinical aspects of NO. inhalation therapy are included. Volume 862
covers methods relating to the generation of NO-. In Section I the chemistry
and biology of NO., methods for its detection, and NO. donors are covered.
Section II covers NO. synthase--its purification, assay of the activity of
endogenous and inducible forms of NO. synthase, its hemoprotein homol-
ogy, the tissue distribution of NO" synthase, and its molecular cloning
and expression.
In bringing these volumes to fruition, credit must be given to experts
in various specialized fields of NO. research who provided contributions
and to those who helped select the authors to provide the state of the art
methodology. The topics and methods included in these volumes were
chosen on the excellent advice of the volume advisors, Bruce N. Ames,
Joseph Beckman, Enrique Cadenas, Victor Darley-Usmar, Bruce Freeman,
iiix
xiv ECAFERP
Barry Halliwell, Louis J. Ignarro, Hiroe Nakazawa, William Pryor, and
Helmut Sies, to whom I extend my sincere thanks and most grateful appreci-
ation.
RETSEL REKCAP
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV II. Preparation and Assay of Enzymes
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV III. Preparation and Assay of Substrates
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV IV. Special Techniques for the Enzymologist
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV V. Preparation and Assay of Enzymes
Edited by SIDNEY P. KClWOLOC DNA NATHAN O. KAPLAN
EMULOV VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VII. Cumulative Subject Index
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VIII. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD DNA VICTOR GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited by WILLIS A. WOOD
EMULOV X. Oxidation and Phosphorylation
Edited by RONALD W. KOORBATSE DNA MAYNARD E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited by C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
by Edited J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV° Steroids and Terpenoids
Edited by DNOMYAR B. NOTYALC
EMULOV XVI° Fast Reactions
Edited by HTENNEK NITSUK
XV
xvi
METHODS IN ENZYMOLOGY
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and )B
Edited yb HERBERT TABOR DNA CELIA WHITE TABOR
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, dna )C
by Edited DONALD B. McCoRMICK DNA LEMUEL D. WRIGHT
EMULOV XIX. Proteolytic Enzymes
by Edited GERTRUDE E. NNAMLREP DNA LASZLO DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited yb MOLDAVE K~VIE DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by WILLIAM B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited by SAN PIETRO ANTHONY
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by PIETRO SAN ANTHONY
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by VICTOR GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE MOLDAVE DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by SIDNEY REHCSIELF DNA PACKER LESTER
EMULOV XXXII. Biomembranes (Part B)
Edited by SIDNEY REHCSIELF DNA PACKER LESTER
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by MARTHA G. DENNIS DNA EDWARD A. DENNIS
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited yb WILLIAM B. JAKOBY DNA MEIR WILCHEK
EMULOV XXXV. Lipids (Part B)
Edited by JOHN M. NIETSNEWOL
xvii
METHODS IN ENZYMOLOGY
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL G. HARDMAN DNA BERT W. O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited yb JOEL G. NAMDRAH DNA BERT W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. WOOD
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. WOOD
EMULOV XLIII. Antibiotics
by Edited JOHN H. HASH
EMULOV XLIV. Immobilized Enzymes
Edited by KLAUS HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited by LASZLO DNAROL
EMULOV XLVI. Affinity Labeling
Edited by WILLIAM B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XLVII. Enzyme Structure (Part E)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited yb VICTOR GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited yb PATRICIA A. HOFFEE DNA MARY ELLEN SENOJ
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA LESTER REKCAP
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited yb SIDNEY REHCSIELF DNA LESTER REKCAP
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA LESTER REKCAP