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NIRRH Annual Report 2012-13_Intro Pages.indd PDF

268 Pages·2014·15.7 MB·English
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TM Typeset & printed at: UNIK PRINTERS PVT. LTD. Email: [email protected] www.indiaprints.com Annual Report 2012 - 2013 2012 - 2013 National Institute for Research in Reproductive Health On the Cover: (Clock wise from Top Left Corner): 1. Community Sensitization Meeting 2. Immunofluorescence of T-complex protein-1(TCP-1) in control stage VII-VIII seminiferous tubules of the rat testis. 3. Session with adolescent girls 4. Immunohistochemical localization of HSP90 in the antral follicle of normal mouse ovary 5. Figure shows balanced viaprical translocation between chromosomes 1 and 9 in a female carrier presently with recurrent spontaneous abortions. Limited number of copies of the Annual Report are available from: Director : National Institute for Research in Reproductive Health, Parel, Mumbai 400012, India Tel.: (022)24192002, 24192100 E-mail: [email protected] Fax: (022) 24139412 Website: www.nirrh.res.in No part of the report should be reproduced in part or whole without prior permission of the Director, NIRRH. Annual Report 2012 - 2013 CONTENTS From the Desk of the Director Summary of Research Activities i – xviii 1. Expanding Contraceptive Choices 1 2.1 Infertility and Reproductive Disorders – Female 10 2.2 Infertility and Reproductive Disorders – Male 39 3. Reproductive Tract Infections 63 4. Menopause and Osteoporosis 89 5. Maternal and Child Health 93 6. Adolescent Reproductive Health 97 7. Genetic Disorders 100 8. Structural Biology and Biomedical Bioinformatics Center 106 9. Stem Cell Biology 116 10. Preclinical and Reproductive and Genetic Toxicology 126 11. Sexual and Reproductive Health –HIV Linkages 132 12. Reproductive Cancers 136 13. Research Support Facilities 140 14. Publications 145 15. Meetings/Conferences/Seminars/Workshops Attended 153 16. Capacity Building 159 17. Honors and Awards 162 18. Members of Advisory Committees 163 19. Extramurally Funded Projects 169 20. Staff and Students 176 21. From the Desk of the Director (Hindi) 181 22. Activities for year 2012- 2013 183 National Institute for Research in Reproductive Health Annual Report 2012 - 2013 From the Desk of the Director… I am gratified to present this report on the research activities of the NIRRH and GRC for the year 2012-2013. As in the previous years, the institute continued to strive towards the excellence in research on basic, clinical, operational, epidemiological and socio-behavioural aspects of reproductive health. During the year, we have moved few inches closer to accomplishing our mandate i.e. increasing contraceptive choices; preventing unsafe abortions, making pregnancy and child birth safer; understanding the causes of various reproductive disorders including infertility, detecting and preventing RTIs/STIs/HIV/AIDS; improving quality of life of post- menopausal women; and devising strategies for reducing gender based violence. During the year, recognition and appreciation came our way in the form of awards and as always, international recognition as publications in high impact international journals. We, at NIRRH, are cognizant of the Council’s goal i.e. to bring the benefits of research to people. User friendly and affordable kits, developed for assessment of the sperm quality, are being distributed to get the end-users’ report. A PCR based technology for the detection of Chlamydia is also available for commercialization. Four urinary hormone (estrone glucuronide, preganaediol glucuronide, LH and FSH) based tests for fertility assessment is being prepared. These kits will be provided to HLL Life-care Limited for clinical validation and mass scale production. During the year, 5 applications were submitted for patents. Researchers at the institute have developed a formulation with a potential to prevent HIV entry into vaginal epithelial cells and PBMCs. A multicentric study has also been initiated to validate the utility of a peptide based test, developed at the institute, for an early detection of endometriosis. Human leukocyte antigen (HLA) based molecular markers, with a potential to predict cervical cancer in HPV infected women, have also been identified. The multidisciplinary bone health clinic has been functional. This research- cum- community based clinic has started catering to the health needs of low socio-economic strata, with special emphasis on prevention and treatment of osteoporosis. The institute has played a pivotal role in initiating ARSH services at 8 PHCs in Maharashtra. It is indeed satisfying to report that now these ARSH clinics have grown and are self- sustained. The institute had submitted guidelines for prevention and management of infertility in the public health care system to the health ministry in April, 2012. To strengthen the operational aspect of the guidelines, a new study has been undertaken to assess the services for prevention and management of infertility in the primary health care system. It gives me immense pleasure to witness the Genetic Research Centre (GRC) going from strength to strength in last 4 years. The bustling 2nd floor corridor, with patients from different parts of the country, testifies a new life infused into the GRC. GRC has also become prolific with regard to publishing research data. The Center has assumed status of National Referral Center and been designated as one of the ICMR Task Force Centers in Human Genetics and for Lysosomal Diseases. Now, its faculty is making efforts to diagnose several morbid and fatal disorders such as infantile hyalinosis, infantile malignant osteoporosis at prenatal stage. Human resource development is one of our very important societal obligations. During the year, 9 students received their doctorate degree. We also disseminate knowledge on research methodologies, observations and advancements to young students, teachers and researchers at various stages of their career. During the year an international conference on millineum developmental goals related to reproductive health, was organized. In addition, nine scientific meetings were conducted in the areas related to RTIs, pharmacovigilance, bioinformatics, maternal and newborn health and medical genetics. We earnestly appreciate support from the Secretary, Department of Health Research, Government of India and Director General, ICMR; Joint Secretory and Sr Deputy Director General (Administration), ICMR; Joint Secretary and Senior Financial Advisor, ICMR and Staff of the Reproductive and Child Health Division, ICMR. Their ceaseless support and encouragements always make our tasks less trying. In essence, we continued our research endeavors aimed towards the betterment of reproductive health. Challenges were galore, but so were the rewards, which make us more determined to tread our path with fervor and enthusiasm. Dr. Sanjiva D. Kholkute Director National Institute for Research in Reproductive Health Annual Report 2012 - 2013 SUMMARY OF RESEARCH ACTIVITIES 1. EXPANDING CONTRACEP- 1.3 Studies with Sperm Proteins for TIVE CHOICES Development of Antifertility Vaccine Immunobiologically mimicking Synthetic Peptides of 80kDa Human Sperm Antigen (80kDa HSA) and 1.1 Identification and Characterization of human Seminal Plasma Inhibin (hSPI) have been Sperm Antigens Using Multifaceted Approach found to be the potential candidates for development of antifertility vaccine. Synthetic Peptide1 of 80kDa HSA Using differential proteomics, several sperm proteins induced immunological infertility in male rats, rabbits were identified and one such protein is Liprin α-3. This and marmosets. Active immunization with synthetic is the first report of liprin α-3 on sperm. It was seen Peptide1 using Muramyl Dipeptide (MDP) as an at 133 kDa in both testicular and epididymal extract. adjuvant and emulsified with squalene and It is an integral protein anddevelopmentally regulated arlaceelicitedelicited a gradual increase in antibody titer by estrogen and conserved across different mammalian and animals failed to impregnate normal fertile females. species. It localizes with golgi marker (gm-130) in The animals are being investigated for regaining testicular cells indicating its involvement in acrosomal fertility with decline in antibody titer. Immunized biogenesis. Polyclonal antibody against Liprin α3 animals did not show changes in their biochemical showed reduced acrosomal reaction. and hematological parameters.The synthetic Peptide1 investigated for preclinical safety in collaboration with 1.2 Harnessing the Superfluity of National Institute of Nutrition was found safe following acute toxicity tests. Reproductive Tract Fluids: Identification of Client Proteins to Testis Specific Antigen, TSA70 Active immunization with N-terminal 17 amino acid Peptide (R-17) of hSPI also elicited gradual increase Studies were initiated to evaluate the effect of male in antibody titer in male marmosets. The animals failed and female reproductive tract fluid proteins on the to impregnate normal fertile females and these animals sperm tail protein Testis Specific Antigen (TSA- regained fertility with decline in antibody titer. The 70), which is a component of the Outer Dense Fibers R-17 peptide is being further investigated for preclinical (ODFs) and is known to interact with a cytokine, efficacy and safety. Macrophage Migration Inhibitory Factor (MIF), a 12.5 kDaepididymal fluid protein. This may help in sperm maturation during epididymal transit by inducing zinc 1.4 Study on Determinants of Vasectomy elimination and affecting the amount of free sulphydryl Acceptance in a Block of Thane District in groups in sperm flagella. Studies are underway to Maharashtra determine interaction with such MIF-like client proteins found in the epididymal, uterine and oviductal fluids The overall objective of the study is to investigate using the recombinant TSA-70 variant 2 [rTSA70(2)]. the determinants of comparatively high acceptance of vasectomy in Jawhar block in Thane district of Surface Plasmon Resonance studies with rTSA70(2) as Maharashtra State. bait showed a oxidoreductase based interaction with the reproductive tract fluid proteins. Based on the finding, Four groups were interviewed in Jawhar block: (i) CNBr coupling based pull down experiments were vasectomy acceptors and their spouses (ii) tubectomy undertaken to identify epididymal fluid client proteins acceptors and their spouses, community leaders, to rTSA 70(2). The study was limited to small molecular motivators and health care providers. Systemic weight proteins in the range of <25kDa. Bound random sampling was applied on R3 registers records protein(s) were eluted with and resolved on a 10 % SDS for vasectomy and tubectomy acceptors in both the PAGE gel. The silver stained profile consistently shows blocks. In addition two FGDs in each block, one each three bands, one at 66 kDa and two bands between 17 with vasectomy and tubectomy acceptors were also and 26 kDa. The identity of these proteins is yet to be conducted. established and is underway. Major observations based on vasectomy acceptors’ data from Jawhar block are as follows: (i) Early i National Institute for Research in Reproductive Health marriage, early completion of family and early adoption household was contacted to screen women with unmet of permanent FP method seems to be norm in the need for family planning. House-listing and rapid community; (ii) low awareness about spacing methods assessment survey was carried out simultaneously to and low utilization; (iii) high awareness on vasectomy identify and screened eligible women with unmet need among males, major source being paramedicals, friends of family planning. Total samples of 568 women with and PHC doctors; (iv) good agreement among couples unmet need for family planning were selected. One fifth on acceptance of vasectomy as 79% took joint decision; (19.7%) of women had ever experienced violence from (v) major reason on acceptance by male is the concern their husbands. Data collection in Tunga village is in for wife’s health, wife also aware of health risks to her, progress. male responsibility and knowledge of NSV as safe and simple procedure. 1.7 Acceptability and Feasibility of using Female Condom in Family Planning Setting 1.5 Gender Equity-Focused, Male-Centered Female condoms are estimated to be highly effective Family Planning for Rural India in preventing pregnancy and HIV/STIs. Introducing The study aims to enhance young couple’s contraceptive and integrating female condom in family planning knowledge and acceptance of spacing methods through programme will expand the contraceptive choices for gender equity focused family planning interventions women and also preventing HIV/STIs. There is no delivered by the village health care providers. Key literature available in Indian context on acceptability components of Counselling Husbands to Achieve of female condom and perception of women in family Reproductive Health and Marital Equity (CHARM) planning settings. intervention were identified and survey instrument, The objectives are: i) to study awareness and intervention manual and Information, Education and acceptability of female condom among the couples Communication (IEC) material was developed and using contraception. ii) To understand attitudes and tested in the field. All these documents were translated perceptions of the couples towards female condom. It is into local language (Marathi). Subsequently, research a clinic based prospective study, involving 120 eligible and pilot intervention was carried out. Based on pilot couples using contraceptive methods such as Oral pills, study, refinement of CHARM intervention model and CuT, and Male/Female sterilization. The present study evaluation tools were assessed. will explore the acceptability of female condom and A Baseline survey and intervention program was future possibility of introducing it in family planning initiated in two PHC villages and three days training programme. program was conducted to train 12 private health care practitioners in three PHC villages. Two refresher training programs were conducted for the village health 2. INFERTILITY care providers for two cohorts. A total of 1080 young married couples from experimental and control (469 and 611 couples respectively) villages have been part 2.1 Female Infertility of the study. A Polycystic Ovary Syndrome 1.6 Intervention to Enhance Acceptance of Contraceptive Use among Couples by Reducing 2.1.1 A Genetic Analysis of Polycystic Ovary Domestic Violence from Husband Syndrome (PCOS) with Special Emphasis on The study aims to understand the effectiveness of an Genes involve in Insulin Resistance intervention to improve contraceptive use among couples where the woman has reported of experiencing Polycystic ovary syndrome (PCOS) is a complex domestic violence. The study was carried out in two polygenic disorder in women. Insulin resistance low income areas (Kajupada and Tunga Village) in is a major pathogenic factor and related genes are Mumbai. The study area Kajupada was divided into suggested to be candidate genes. During the reporting four clusters. Systematic random sampling i.e every 6th year, we have completed genotyping of coding region ii

and two proteins Methylmalonate-semialdehyde dehydrogenase semialdehyde dehydrogenase Ajanta Narvekar,. Consultant Infertility. Duration.
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