Nicotinic Acetylcholine Receptors in the Nervous System

431 Pages·1988·26.752 MB·English

by  Paul B. S. Clarke (auth.), Francesco Clementi, Cecilia Gotti, Emanuele Sher (eds.)

NATO ASI Series
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CELL TO CELL SIGNALS IN PLANTS AND ANIMALS
This book contains the proceedings of a NATO Advanced Research Workshop held within the
activities of the NATO Special Programme on Cell to Cell Signals in Plants and Animals, running
from 1984 to 1989 under the auspices of the NATO Science Committee.
The books published as a result of the activities of the Special Programme are:
Vol. 1 : Biology and Molecular Biology of Plant-Pathogen Interactions. Edited by J.A. Bailey. 1986.
Vol. 2: Glial-Neuronal Communication in Development and Regeneration.
Edited by H.H. Althaus and W. Seifert. 1987.
Vol. 3: Nicotinic Acetylcholine Receptor: Structure and Function. Edited by A. Maelicke. 1986.
Vol. 4: Recognition in Microbe-Plant Symbiotic and Pathogenic Interactions.
Edited by B. Lugtenberg. 1986.
Vol. 5: Mesenchymal-Epithelial Interactions in Neural Development.
Edited by J. R. Wolff, J. Sievers, and M. Berry. 1987.
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Edited by T. M. Konijn, P.J. M. Van Haastert, H. Van der Starre, H. Van der Wei,
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Vol. 9: Modification of Cell to Cell Signals During Normal and Pathological Aging.
Edited by S. Govoni and F. Battaini. 1987.
Vol. 10: Plant Hormone Receptors. Edited by D. Klambt. 1987.
Vol. 11: Host-Parasite Cellular and Molecular Interactions in Protozoal Infections.
Edited by K.-P' Chang and D. Snary. 1987.
Vol. 12: The Cell Surface in Signal Transduction.
Edited by E. Wagner, H. Greppin, and B. Millet. 1987.
Vol. 19: Modulation of Synaptic Transmission and Plasticity in Nervous Systems.
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Vol. 20: Amino Acid Availability and Brain Function in Health and Disease. Edited by G. Huether. 1988.
Vol. 21: Cellular and Molecular Basis of Synaptic Transmission. Edited by H. Zimmermann. 1988.
Vol. 23: The Semiotics of Cellular Communication in the Immune System.
Edited by E. E. Sercarz, F. Celada, N. A. Mitchison, and~. Tada. 1988.
Vol. 24: Bacteria, Complement and the Phagocytic Cell. Edited by F.C. Cabello and C. Pruzzo. 1988.
Vol. 25: Nicotinic Acetylcholine Receptors in the Nervous System.
Edited by F. Clementi, C. Gotti, and E. Sher. 1988.
Nicotinic Acetylcholine
Receptors in the Nervous
System
Edited by
Francesco Clementi
Cecilia Gotti
Emanuele Sher
CNR Center of Cytopharmacology
Department of Medical Pharmacology
University of Milano
Via Vanvitelli 32
1-20129 Milano, Italy
Springer -Verlag
Berlin Heidelberg New York London Paris Tokyo
Published in cooperation with NATO Scientific Affairs Division
Proceedings of the NATO Advanced Research Workshop on Nicotinic Acetylcho
line Receptors in the Nervous System held in Venice, Italy, April 18-21 ,1988
Library of Congress Cataloging-in-Publication Data. NATO Advanced Research Workshop on Nicotinic
Acetylcholine Receptors in the Nervous System (1988: Venice, Italy) Nicotinic acetylcholine receptors in the
nervous system 1e dited by Francesco Clementi, Cecilia Gotti, Emanuele Sher. p. cm.-(NATO ASI series.
Series H, Cell biology; vol. 25) Proceedings of the NATO Advanced Research Workshop on Nicotinic
Acetylcholine Receptors in the Nervous System held in Venice, Italy, April 18-21 , 1988"-T.p. verso.
"Published in cooperation with NATO Scientific Affairs Division." Includes bibliographies and indexes.
ISBN-13: 978-3-642-74169-2 e-ISBN-13: 978-3-642-74167-8
001: 10.1007/978-3-642-74167-8
1. Nicotinic receptors-Congresses. 2. Acetylcholine-Receptors-Congresses. I. Clementi, Francesco.
II. Gotti, Cecilia. III. Sher, Emanuele. IV. North Atlantic Treaty Organization. Scientific Affairs Division. V. Title.
VI. Series. [DNLM: 1. Receptors, Cholinergic-congresses. 2. Receptors, Nicotinic-congresses.
WL 102.8 N2795n 1988] QP364.7.N3641988 615'.785-dc 19 DNLM/DLC for Library of Congress
88-38978
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© Springer-Verlag Berlin Heidelberg 1988
50ftcover reprint of the hardcover 15t edition 1988
2131/3140-543210-Printed on acid-free paper
- CONTENTS -
PREFACE
SCIENTIFIC COMMlTfEE
WORKSHOP PARTICIPANTS
CONFERENCE PHOTOORAPH
BIOCHEMICAL AND PHARMACOLOGICAL CHARACTERIZATION OF
NEURONAL NICOTINIC RECEPTORS
AUTORADIOGRAPHIC MAPPING OF PUTATIVE NICOTINIC CHOLINOCEPfOR IN
MAMMALIAN BRAIN
P.B.S. Clarke 1
FUNCTIONAL STUDIES OF NEURONAL NICOTINIC RECEPfORS UTILIZING
KAPPA-NEUROTOXINS
V.A. Chiappinelli, S.E. Dryer, E.M. Sorenson, K.M. Wolf, G.A. Grant, S.-J.
Chen, J.M. Nooney, J.J. Lambert and R.C. Hider 15
CHARACTERIZATION AND LOCALIZATION OF GANGLIONIC NICOTINIC
RECEPfORS USING NEURONAL BUNGAROTOXIN
R.E. Zigmond and R.H. Loring 31
ALPHA-BUNGAROTOXIN AND PRESYNAPTIC NICOTINIC RECEPTORS:
FUNCTIONAL STUDIES
S. Wonnacott, J. Irons, G.G. Lunt, C.M. Rapier and E.X. Albuquerque 41
EVIDENCE FOR FUNCTIONAL AND STRUCTURAL DIVERSITY OF NICOTINIC
ACETYLCHOLINE RECEPTORS
Lukas R.J. 61
BIOCHEMICAL AND MOLECULAR BIOLOGY APPROACHES TO CENTRAL
NICOTINIC ACETYLCHOLINE RECEPfORS
I. Hermans-Borgmeyer, E. Sawruk, P. Schloss, E.D. Gundelfinger and H.
Ben 77
ALPHA-BUNGAROTOXIN RECEPfOR IN A HUMAN NEUROBLASTOMA CELL LINE
C. Gotti, E. Sher, D. Fornasari, B. Chini, A. Esparis Ogando, F. Clementi 89
CHARACTERIZATION OF THE ALPHA-BUNGAROTOXIN BINDING PROTEIN FROM
PC12 CELLS
G. Kemp 105
IDENTIFICATION OF SURFACE DOMAINS OF THE NICOTINIC ACETYLCHOLINE
RECEPfOR MOLECULE BY THE USE OF ANTIBODIES AND TOXINS.
B.M. Conti-Tronconi, A. Kokla, A. Maelicke, R. Milius, M.A. Raftery, S.R.
Spencer, F. Tang and S. Tzartos 119
HETEROGENEITY OF NICOTINE ACTIONS IN THE RAT CEREBELLUM
R. de La Garza, B.J. Hoffer, and R. Freedman 137
VI
DISTRIBUTION OF NICOTINIC RECEPTORS IN THE HUMAN BRAIN:
QUANTITATIVE AUTORADIOGRAPHY USING 3H-NICOTINE
A. Adem, P.-G. Gillberg, S.S. Jossan, V. Sara and A. Nordberg 143
DISTRIBUTION OF 3H-N-METHYLCARBAMYLCHOLINE BINDING SITES IN
RAT BRAIN: CORRELATION WITH NICOTINIC AUTORECEPTORS.
P.A. Lapchak, D.M. Araujo, R. Quirion, B. Collier 149
MOLECULAR BIOLOGY OF AChR
STRUCfURE OF NEURONAL NICOTINIC RECEPTORS
J. Lindstrom, P. Whiting, R. Schoepfer, M. Luther and B. Casey 159
THE NICOTINIC ACETYLCHOLINE RECEPTOR GENE FAMILY
S. Heinemann, J. Boulter, E. Deneris, J. Connolly, P. Gardner, E.
".' Wada, K. Wada, M. Ballivet, L. Swanson and J. Patrick 173
NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR GENES IN THE AVIAN
GENOME
P. Nef, C. Oneyser, C. Alliod, S. Couturier and M. Ballivet 193
INVERTEBRATE AChR
NICOTINIC ACETYLCHOLINE RECEPTORS IN INSECT GANGLIA AND MAM
MALIAN BRAIN: SOME COMPARISON
D.R.E. Macallan, S. Wonnacott and G.G. Lunt 199
IDENTIFICATION OF NICOTINIC ACETYLCHOLINE RECEPTORS FROM THE
NERVOUS SYSTEM OF INSECTS
H. Breer and W. Hanke 213
PHARMACOLOGY OF A LOCUST THORACIC GANGLION SOMAL NICOTINIC
ACETYLCHOLINE RECEPfOR
J.A. Benson 227
SYNAPTIC AND EXTRASYNAPTIC NEURONAL NICOTINIC RECEPTORS OF
INSECTS
D. B. Sattelle 241
PHARMACOLOGY, CLONING AND EXPRESSION OF INSECT NICOTINIC
ACEfYLCHOLlNE RECEPTORS
J. Marshall, J.A. David, M.G. Darlison, E.A. Barnard and D.B. Sattelle 257
AChR INVOLVEMENT IN PHYSIOLOGY AND PATHOLOGY
REGULATION OF NEURONAL ACETYLCHOLINE RECEPTORS BY CELL-CELL
INTERACTIONS
D.K. Berg, R.T. Boyd, S.W. Halvorsen, L.S. Higgins, M.H. Jacob, J.F.
Margiotta and A.E. McEachern 283
VII
PHARMACOLOGICAL MODULATION OF BRAIN NICOTINIC BINDING SITES
L.G. Costa 299
REGULATION OF BEHAVIORAL AND PHYSIOLOGICAL SENSITIVITY TO NICOTINE
BY BRAIN NICOTINIC RECEPTORS
A.G. Collins and M.J. Marks 317
HEfEROGENEOUS CHOLINERGIC NICOTINIC RECEPTORS IN THE CNS
A. Nordberg, A. Adem, L. Nilsson, L. Romanelli and X. Zhang 331
CHOLINERGIC NEUROPATHOLOGY AND NICOTINIC RECEPTOR BINDING IN THE
HUMAN BRAIN
E.K. Perry, C.J. Smith, l.H.Xuereb, A.B. Keith, R.H. Perry 351
THE CHOLINERGIC RECEPTOR SYSTEM IN THE FRONTAL LOBE OF ALZHEIMER
PATIENTS
E. Giacobini, P. De Sarno, M. Mcllhany, B. Clark 367
DEVELOPMENTAL REGULATION AND MODULATION OF NEURONAL NICOTINIC
ACETYLCHOLINE RECEPTOR CHANNELS
L.K. Simmons, B.L. Moss, S.M. Schuetze and LW. Role 379
IMMUNOCYTOCHEMICAL LOCALIZATION OF NICOTINIC RECEPTORS IN THE
FROG RETINA: PHYSIOLOGICAL CORRELATES
N. Bonaventure, B. lardon, 1. Sahel, G. Roussel and H. Yucel 393
AUTHORS INDEX 409
SUBJEcrs INDEX 411
PREFACE
The acetylcholine nicotinic receptor is an ionic channel whose aperture is
directly controlled by acetylcholine. It is a key molecule in the chemical
communication between nerve cells and between nerve cell and muscle.
The structure and function of muscular nicotinic receptors have been unraveled
in recent years and its beauty and mysteries were reviewed in the Santorini NATO
ARW organized by Dr. Maelicke in 1986. The neat, linear structure of this molecule
and its conservation throughout evolution, from bacteria to humans, have led to
the suggestion that it has reached the optimal structure for performing its
function. But when scientists began to look at the nicotinic receptor in the
nervous system, they found several surprises.
From the beginning, pharmacological and physiological experiments, have made
it clear that the functional characteristics of neuronal nicotinic receptors are
substantially different from those of muscle receptors. Furthermore, recent
sophisticated techniques such as patch clamp and gene cloning have revealed that
the nicotinic receptor in the nervous system is not a single molecule but
(although there are some important exceptions) a large family of similar
molecules, which have in common the property of binding nicotinic agonists or
antagonists.
Over the last few years, the physiological implications of nicotinic receptors have
been reevaluated on the basis of more precise behavioural and pharmacological
techniques. Moreover, the involvement of nicotinic receptors in degenerative
pathologies of the eNS, such as Parkinson's and Alzheimer's disease, has also been
discovered very recently.
In collaboration with Drs. Eric Barnard and Hans Breer, we thought that the time
had come to gather together all the scientists working on the nicotinic receptors
of the nervous system (from molecular biologists to physiologists, from
pharmacologists to clinicians) in order to clarify the basic characteristics of the
molecule and to open up new lines of research. Our scientific knowledge would
certainly be extended in a shorter time if everyone worked together.
The Venice workshop held in the superb palazzo of the Fondazione Querini
Stampalia and sponsored by the Fondazione Emilio Trabucchi was an ideal setting
for an informal and fruitful meeting.
Representatives from nearly all the laboratories working on this subject were
present and the atmosphere of Venice itself helped to create the appropriate
conditions for informal discussion and the establishement of friendly relations.
IX
The valuable contributions presented at the meeting are collected in this book. It
will certainly become the basic reference work for scientists active in the field of
the nicotinic receptors of the nervous system, since it contains the most up to date
information about the different scientific aspects of this fascinating new area,
written by the most experienced researchers in the field.
The meeting was made possible by a substantial grant from the NATO Special
Program on Cell-to-Cell Signals, and by the generous support of Fidia Research
Laboratories. Our stay in Venice was organized by the expert of Venetian life, Dr.
Giorgio Busetto, Director of the Fondazione Querini Stampalia, and his staff who
opened palazzo Querini to "nicotinic" scientists, suggested the social program .and
contacted local organizations.
Mrs. Ida Ruffoni in particular, but also all the other colleagues from our
laboratory, contributed with their enthusiasm and dedication, to creating the
atmosphere and the success of the meeting.
We are also indebted to the CNR (the Italian National Research Council), the
Regional authorities of Veneto and the local authorities of Venice, Crinos SpA, ISF
SpA, Italfarmaco, Sigma Tau and Zambon SpA, which made our life as organizers
easier and the stay of the scientists attending the meeting more pleasant.
We hope that this meeting has opened the way for a long and exciting series of
future adventures in the discovery of the still mysterious facets of these
molecules.
Francesco Clementi
Cecilia Gotti
August 1, 1988. Emanuele Sher
Scientific committee
Eric A. Barnard MRC Mol. Neurobiology Unit
University of Cambridge
Medical School
Cambridge, England
Hans Breer Dept. of Physiology
University of Osnabruck
Osnabruck, FRG
Francesco Clementi Dept. of Pharmacology
University of Milan
Milan, Italy
Cecilia Gotti CNR Center of Cytopharmacology
University of Milan
Milan, Italy
Emanuele Sher CNR Center of Cytopharmacology
University of Milan
Milan, Italy