Natural Product Experiments in Drug Discovery

569 Pages·2022·20.286 MB·English

by  Karuppusamy Arunachalam, Xuefei Yang, Sreeja Puthanpura Sasidharan

Karuppusamy Arunachalam
Xuefei Yang · Sreeja Puthanpura Sasidharan
Editors
Natural Product
Experiments
in Drug Discovery
S P H
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Natural Product Experiments
in Drug Discovery
Edited by
Karuppusamy Arunachalam and Xuefei Yang
Key Laboratory of Economic Plants and Biotechnology and the Yunnan Key Laboratory for Wild
Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming,
People’s Republic of China; Southeast Asia Biodiversity Research Institute, Chinese Academy
of Sciences, Yezin, Nay Pyi Taw, Myanmar; Yunnan International Joint Laboratory
of Southeast Asia Biodiversity Conservation, Menglun, Yunnan, China
Sreeja Puthanpura Sasidharan
Department of Botany, NSS College, Nemmara, Palakkad, Kerala, India
Editors
KaruppusamyArunachalam XuefeiYang
KeyLaboratoryofEconomicPlantsand KeyLaboratoryofEconomicPlantsand
BiotechnologyandtheYunnanKey BiotechnologyandtheYunnanKeyLaboratoryfor
LaboratoryforWildPlantResources WildPlantResources
KunmingInstituteofBotany,ChineseAcademy KunmingInstituteofBotany,ChineseAcademy
ofSciences ofSciences
Kunming,People’sRepublicofChina Kunming,People’sRepublicofChina
SoutheastAsiaBiodiversityResearchInstitute SoutheastAsiaBiodiversityResearchInstitute
ChineseAcademyofSciences ChineseAcademyofSciences
Yezin,NayPyiTaw,Myanmar Yezin,NayPyiTaw,Myanmar
YunnanInternationalJointLaboratory YunnanInternationalJointLaboratory
ofSoutheastAsiaBiodiversityConservation ofSoutheastAsiaBiodiversityConservation
Menglun,Yunnan,China Menglun,Yunnan,China
SreejaPuthanpuraSasidharan
DepartmentofBotany
NSSCollege,Nemmara
Palakkad,Kerala,India
ISSN1949-2448 ISSN1949-2456 (electronic)
SpringerProtocolsHandbooks
ISBN978-1-0716-2682-5 ISBN978-1-0716-2683-2 (eBook)
https://doi.org/10.1007/978-1-0716-2683-2
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Preface
Medicinal plants have an unbelievable history in terms of serving humanity in almost all
continentsoftheworld.Traditionalhealerstransferthisincredibleknowledgefromgenera-
tion to generation. Even modernity or cultural revolutions have not altered the in-depth
wisdom of this natural medical paradigm. Furthermore, the hunt for novel medications to
treatarangeofdisordersusingchemicalsynthesisandothercontemporarytechnologieshas
not proven fruitful due to their side effects and expensive treatments accompanied by the
advent of novel infectious diseases, the spread of lifestyle disorders such as cancer and
diabetes,andincreasingmultidrugresistanceinbacteria.Thishassparkedincreasedinterest
in the identification of potential therapeutic compounds from medicinal plants on their
pharmacologicalrationaleandphytochemicalstudiesinlightoftraditionaluses.Moreover,
herbalmedicineisnowwidelyrecognizedasagenuinealternativetherapeuticsysteminthe
formofmedicines,functionalfoods,andsoon,amovementrecognized andsupportedby
the World Health Organization. There are many medicinal plants that have yielded drugs
like vincristine and vinblastine (from Catharanthus roseus (L.) G.Don), d-tubocurarine
(from Chondrodendron tomentosum Ruiz & Pav.), pilo-carpine (from Pilocarpus jaborandi
Holmes),reserpine(Rauvolfiaserpentina(L.)Benth.exKurz),andsoon.
On theother hand, as themajority ofancient medical treatises remain unreported and
uncharacterized,thefocusofthisvolumeisondescribingthewholerangeofevidence-based
complementarymedicineandvariousbio-analyticaltechniquesusedtodefinethebotanical
products.Wehaveattemptedtogatherrecentworkandcurrentdevelopmentsinthefieldof
contemporary phytomedicine as well as their future possibilities in human healthcare.
Although there are several books on medicinal plants and phytocompounds, this book has
uniquecontributionsintheformofchaptersfromexpertsintheconceptofphytomedicine
and screening biological activities explained with diverse hyphenated techniques, as well as
discussedissuesrelatedtoherbalmedications,suchasefficacy,adulteration,safety,toxicity,
regulations, and drug delivery, among others. It is designed to be referable for students,
professors,andresearchersinuniversities,R&Dinstitutes,pharmaceuticalandherbalenter-
prises,andhealthorganizations.
Weconveyourheartfeltgratitudeandappreciationtoallcontributorsfor theirprompt
answers,goodandup-to-datecontributions,andpersistentcollaboration.Weexpressdeep
gratitudetoProf.T.Parimelazhagan,Head,DepartmentofBotany,BharathiarUniversity,
India; Prof. Domingos Tabajara de Oliveira Martins, Department of Basic Sciences in
Health, Faculty of Medicine, Federal University of Mato Grosso, Brazil; Prof. Dr. Valter
Araga˜o do Nascimento and Prof. Dr. Rodrigo Juliano Oliveira, Postgraduate Program in
Health and Development of the Midwest Region, Postgraduate Program in Health and
DevelopmentoftheMidwestRegion,FacultyofMedicine(FAMED),FederalUniversityof
MatoGrossodoSul(UFMS),MatoGrossodoSul,Brazil.Prof.MarcoLeonti,Department
of Biomedical Sciences, University of Cagliari, Cagliari (CA), Italy; and Prof. Michael
Heinrich, Centre for Pharmacognosy and Phytotherapy, The School of Pharmacy, Univer-
v
vi Preface
sity of London, who have been a great source of inspiration. We also thank our colleagues
and our research students, for their cooperation and critical suggestions. The technical
support and continued encouragement received from David C. Casey (Senior Editor) and
the book publishing team at Springer Nature, New York, NY, USA, is also acknowledged.
This project work was financially supported by the Chinese Academy of Sciences – Pre-
sident’s International Fellowship for Postdoctoral Research (CAS-PIFI, Reference no.
2020PB0112), Inventory and Database Construction Project of Herbal Medicine, along
with the “Belt and Road Countries” (Grant number 2018FY100700), the Southeast Asia
Biodiversity Research Institute, Chinese Academy of Sciences, Peoples Republic of China
(GrantnumberY4ZK111B01),andtheYunnanProvinceScienceandTechnologyDepart-
ment (Grant number 202203AP140007). Finally, we acknowledge Almighty God, who
providedallthechannelstoworkincohesionandcoordinationrightfromtheconceptionof
theideatothedevelopmentofthefinalversionofthisbook,NaturalProductExperiments
inDrugDiscovery.
Kunming,China KaruppusamyArunachalam
Kunming,China XuefeiYang
Palakkad,Kerala,India SreejaPuthanpuraSasidharan
Contents
Preface ..................................................................... v
Contributors................................................................. xi
1 Phytochemical,Antioxidant,andFlavonoidInvestigationofMethanolic
LeafExtractofPiliostigmathonningii(Schum.) ..... ........ ....... ........ 1
MichaelO.Afolayan,RebeccaO.Omosimua,AdewaleE.Fadeyi,
OnyinyeC.Aguzue,OlayinkaT.Asekun,andOluwoleB.Familoni
2 AComparativeEvaluationofOrganoleptic,Physicochemical,Proximate,
andMineralCompositionofSyzygiumlaetumandSyzygiumoccidentale,
EndemictoWestern Ghats:ASearchforLow-CostNutraceuticals.... ........ 27
V.R.SnehalathaandA.R.Rasmi
3 ChemicalCharacterization,BiologicalActivities,andSomeMedicinal
UsesofDifferentSweetBasil(OcimumbasilicumL.)Genotypes ..... ........ 41
GulsumYaldiz,MahmutCamlica,Yashashree Pradhan,
andAhmadAli
4 EstimationofPrimaryandSecondaryMetabolitesandInVitroFree
RadicalScavengingActivitieswithFicustsjahelaBurm.F.Crude
Extracts.... ........ ....... ........ ....... ....... ........ ....... ........ 63
ThamaraikaniVelmurugan,AmalaDivyaSebasthi,
andSekarThangavel
5 EvaluatingtheLipidProfileandMineralCompositionoftheSeedOil
ofCaesalpiniasappanL.(Caesalpiniaceae).......... ........ ....... ........ 75
S.Nagaraju,T.G.Thippeswamy,andH.RaviKumar
6 PhytochemicalScreening,PhysicochemicalAnalysis,andPowder
CharacterizationofSelectedLitseaSpeciesEndemic
totheSouthernWestern Ghats...... ....... ....... ........ ....... ........ 87
K.UmmuHabeebaandA.R.Rasmi
7 MetaboliteProfilingofBioactiveCompoundsinSelectedSpecies
ofGomphostemmaWall.exBenth.fromtheWesternGhats
byGC-MSAnalysis ........ ........ ....... ....... ........ ..... .. ........ 97
K.SajithaMenonandJohnE.Thoppil
8 PhytochemistryandPharmacologyofAromaticMedicinalPlant
Commiphoracaudata(Wight&Arn.)Engl. ........ ........ ....... ........ 109
Ch.SrinivasaReddy,K.SriRamaMurthy,andK.Ammani
9 UnravellingthePhytochemistryofEthnomedicinalTree
Pajanelialongifolia(Willd.) K.Schum....... ....... ........ ....... ........ 121
C.K.Smitha,P.S.Udayan,andM.Vaishnavi
10 ComparativePhycochemicalInvestigationandAntioxidantContent
ofTwoMarineSeaweeds ........... ....... ....... ........ ....... ........ 131
S.AmalaDivya,V.Thamaraikani,S.Karthikeyan,andT.Sekar
vii
viii Contents
11 EvaluationofNutritional,Antioxidant,andAnti-arthriticActivity
ofHedychiumcoronariumJ.KoenigRhizome....... ........ ....... ........ 147
BenedictMathewsPaul,GowthamKannan,S.Sruthi,
AshwiniLydiaManoharan,GayathriNataraj,
andParimelazhaganThangaraj
12 Phytochemical,Pharmacognostic,andInVitroAcetylcholinesterase
InhibitoryActivitiesofCipadessabacciferaLeaves........... ....... ........ 173
T.L.MarySheejaandGomathiVenkatachalam
13 ComparativeStudyofDrugEfficiencyofSemecarpusanacardium
andTridaxprocumbensAgainstZikaVirus.......... ........ ....... ........ 183
AkshataSharma,A.Aishwarya,S.RiyazSharif,AbhishekBiswal,
S.IvoRomauld,andVivekPazhamalai
14 EvaluationofInVitroAntioxidantActivityofOldenlandiadineshii:
AnEndemicPlantfromtheHillocksofPalakkadDistrict,Kerala,India ....... 203
M.K.Prasanth,C.Chandini,andL.Leeja
15 IdentificationofSecondaryMetabolitesThroughHR-GC-MS:Antibacterial
andAntifungalActivityfromRootFractionsofAlstoniascholaris
(L.)RBr.fromAndhraPradesh,SouthIndia........ ....... ....... .... ..... 215
DevarakondaRamadevi,RadhaRayi,andGangaRaoBattu
16 InVitroModelsonInhibitionofInflammatoryMediatorsbyEthanolic
ExtractofCareyaarboreaRoxb.BarkinLipopolysaccharide-Induced
RAW264.7CellLines...... ........ ....... ....... ........ ....... ........ 231
A.R.BinduandN.A.Aleykutty
17 PharmacologicalElucidationofAntioxidant,Hypoglycemic,
andAnti-InflammatoryPotentialsofPhyllanthuscandolleanus
(WightsARN)Chakrab..... ........ ....... ....... ........ ....... ...... .. 249
MohammedAsiq,GayathriJagadeesan,FrancisJeganRaj,
S.AnishRuban,KasipandiMuniyandi,
andParimelazhaganThangaraj
18 α-GlucosidaseInhibitionandUpregulationofPPARγbyFlavonoid
NaringeninfromTinosporasinensisStem,aPossibleMechanism
ofAntidiabeticActivity ..... ........ ....... ....... ........ ....... ........ 267
BobbyS.PrasadandK.K.Srinivasan
19 PurificationandCharacterizationofaCysteineProteasefromSprouted
LablabpurpureusSeedRadicleExtract:ItsEffectonBloodCoagulation ...... 291
R.S.Thoyajakshi,S.Nagaraju,H.RaviKumar,andD.Poornima
20 EffectofSterilizationAgents,GrowthRegulators,andActivated
CharcoalonCallusCulturesofEvolvulusalsinoides(Linn.)Linn...... ........ 311
PavithraSivarajaandKannanKilavanPackiam
21 Bryophytes:AMyriadHueofBio-resourceswithTherapeutic
Potentialities ....... ....... ........ ....... ..... .. .. ...... ....... ........ 321
G.S.Manoj,G.M.Greeshma,RemyaKrishnan,andK.Murugan
22 RecentBiotechnologicalApproachesfor theEnhancementofArtemisinin
ProductionfromCellCultureSystemofArtemisiaannuaL.:
MedicinalPlantwithPotentAntimalarialProperties.......... ....... ........ 361
GanesanMahendranandLaiqurRahman
Contents ix
23 TheUtilityofNaturalMucilagefromtheMedicinalPlant“Patha”
(Cycleapeltata)asanAlternativeforSolidifyingAgentinCell
GrowthMedia...... ....... ........ ....... ....... ........ ....... ........ 377
AnjanaKrishnan,JomyJoseph,andSudhaKalyanikutty
24 MicrowaveIrradiation-AssistedExtractionandSynthesisofSilver
NanoparticlesUsingTreeTomatoFruitPulp(SolanumbetaceumSendt.)
andDeterminationofInVitroCytotoxicEffectonMCF-7Cells..... ........ 395
MaryJoseph,AarthiJeganathan,andGeethaNatesan
25 CharacterizationofCrystalsIsolatedfromtheCaesalpiniasappanSeeds:
AComparativeInSilicoAnalysisonBioactivityofSappanDiterpenoids....... 413
H.RaviKumar,S.Nagaraju,KiranKrishnamurthy,N.S.Surendra,
SrinivasaraoRaghothama,T.G.Thippeswamy,T.P.N.Hariprasad,
andAnjaliGanjiwale
26 ComputationalAnalysisofNaturalCompoundsasPotentialIsocitrate
Dehydrogenase1Inhibitor for theTreatmentofOvarianCancer..... ........ 427
NirmalaGanesanandAkshataSharma
27 DrugEfficiencyofOroxylumindicumandTaxuswallichiana
AgainstColon-AssociatedDiseases:InSilicoStudies ......... .... ... ...... .. 443
AkshataSharma,VarshaKarikalan,BhuvaneswariSaikumar,
andSakthiselvanPunniavan
28 GasChromatography-MassSpectrometryAnalysisofPhytoconstituents
fromGarciniagummi-guttaSeedExtract:TrypsinInhibition
StudyofExtractandMolecularDockingStudy...... ........ ....... ........ 471
C.K.Sumachirayu,C.G.Kusuma,G.T.Megha,VinodGubbiveeranna,
S.Bhavana,H.RaviKumar,andS.Nagaraju
29 InSilicoAnalysisofAnti-inflammatoryActivityofQuercetin
fromM.calaburaFruit..... ........ ....... ....... ........ ....... ........ 489
K.Preethi
30 PhytochemicalInvestigation,MolecularDockingStudies,andBiological
EfficacyofRhizomeEssentialOilCompositionofCostusspeciosus .... ........ 505
MityThambiandTomCherian
31 FicustalbotiKing:AntinociceptiveandAnti-inflammatoryProperties
ofMethanolExtractofStemBark ...... .... ....... ........ ....... ........ 521
KaruppusamyArunachalam,SreejaPuthanpuraSasidharan,
ThangarajParimelazhagan,andXuefeiYang
32 PhytochemicalandAntioxidantAnalysisofPhellinusadamantinus
andP.hohnelli...... ....... ........ ....... ....... ........ ....... ........ 543
S.Rajeswari,S.Manian,andS.Saikumar
Index ...................................................................... 559