NASA Technical Reports Server (NTRS) 20080007454: Growth stimulation of biological cells and tissue by electromagnetic fields and uses thereof

2.7 MB·English

by  NASA Technical Reports Server (NTRS)

1Il lI1 1ll11111 Il1ll lI11 lIl1 IIIII IIIII 11111 Ill11 IIIII 11111 11111111111111 I1ll
US006673597B2
United States Patent
(12) (io) Patent No.: US 6,673,597 B2
Wolf et al. (45) Date of Patent: Jan. 6,2004
GROWTH STIMULATION OF BIOLOGICAL 3,133,003 A * 511964 Schaefer et al.
CELLS AND TISSUE BY 3,871,961 A * 311975 Gianessi .........
ELECTROMAGNETIC FIELDS AND USES 4,377,639 A * 311983 Lee ......................... 4351299.1
4,487,834 A * 1211984 Brighton ..................... 4351173
THEREOF
4,703,010 A * 1011987 Yunker et al. ........... 4351173.8
4,762,795 A * 811988 Masson ................... 4351173.8
Inventors: David A. Wolf, Houston, TX (US);
4,939,151 A * 711990 Bacehowski et al. ....... 4351402
Thomas J. Goodwin, Friendswood, TX 5,134,070 A * 711992 Casnig .................... 4351173.6
(US) 5,270,205 A * 1211993 Rogalsky
5,316,945 A * 511994 Minuth ...
Assignee: The United States of America as 5,344,454 A * 911994 Clarke et al. ............ 623123.72
represented by the Administrator of 5,919,679 A * 711999 Blackman et al. 4351173.1
the National Aeronautics and Space 6,022,733 A * 212000 Tam et al. ............... 4351287.1
Administration, Washington, DC (US) 6,066,495 A * 512000 Fofonoff et al. ......... 4351289.1
* cited by examiner
Notice: Subject to any disclaimer, the term of this
patent is extended or adjusted under 35
Primary Examinerqavid A. Redding
U.S.C. 154(b) by 0 days.
(74) Attorney, Agent, or Firm-James M. Cate
Appl. No.: 09/798,854 (57) ABSTRACT
Filed: Feb. 28, 2001 The present invention provides systems for growing two or
three dimensional mammalian cells within a culture medium
Prior Publication Data
facilitated by an electromagnetic field, and preferably, a time
US 200210009797 A1 Jan. 24, 2002 varying electromagnetic field. The cells and culture medium
are contained within a fixed or rotating culture vessel, and
Related U.S. Application Data the electromagnetic field is emitted from at least one elec-
trode. In one embodiment, the electrode is spaced from the
Division of application No. 091587,028, filed on Jun. 2, vessel. The invention further provides methods to promote
2000, now Pat. No. 6,485,963.
neural tissue regeneration by means of culturing the neural
Int. Cl? ................................................. C12M 1/10 cells in the claimed system. In one embodiment, neuronal
U.S. C1. .................................. 435/298.2; 4351299.1 cells are grown within longitudinally extending tissue
Field of Search ........................... 4351173.1, 173.8, strands extending axially along and within electrodes com-
4351298.2, 299.1 prising electrically conductive channels or guides through
which a time varying electrical current is conducted, the
References Cited conductive channels being positioned within a culture
medium.
U.S. PATENT DOCUMENTS
2,996,429 A * 811961 Toulmin, Jr. ................ 4351391 25 Claims, 11 Drawing Sheets
1
9
US. Patent
Jan. 6,2004 Sheet 1 of 11 US 6,673,597 B2
US. Patent
Jan. 6,2004 Sheet 2 of 11 US 6,673,597 B2
US. Patent
Jan. 6,2004 Sheet 3 of 11 US 6,673,597 B2
US. Patent
Jan. 6,2004 Sheet 4 of 11 US 6,673,597 B2
FIGURE 4
US. Patent
Jan. 6,2004 Sheet 5 of 11 US 6,673,597 B2
FIGURE 5
US. Patent
Jan. 6,2004 Sheet 6 of 11 US 6,673,597 B2
US. Patent
Jan. 6,2004 Sheet 7 of 11 US 6,673,597 B2
US. Patent
Jan. 6,2004 Sheet 8 of 11 US 6,673,597 B2
US. Patent
Jan. 6,2004 Sheet 9 of 11 US 6,673,597 B2