Nanotoxicity: Methods and Protocols

369 Pages·2019·9.52 MB·English

by  Qunwei Zhang

Methods in
Molecular Biology 1894
Qunwei Zhang Editor
Nano-
toxicity
Methods and Protocols
M M B
ETHODS IN OLECULAR IO LO GY
SeriesEditor
JohnM.Walker
School of Lifeand MedicalSciences
University ofHertfordshire
Hatfield, Hertfordshire,AL109AB,UK
Forfurther volumes:
http://www.springer.com/series/7651
Nanotoxicity
Methods and Protocols
Edited by
Qunwei Zhang
Department of Environmental and Occupational Health Sciences, School of Public Health and
Information Sciences, University of Louisville, Louisville, KY, USA
Editor
QunweiZhang
DepartmentofEnvironmentalandOccupationalHealthSciences
SchoolofPublicHealthandInformationSciences
UniversityofLouisville
Louisville,KY,USA
ISSN1064-3745 ISSN1940-6029 (electronic)
MethodsinMolecularBiology
ISBN978-1-4939-8915-7 ISBN978-1-4939-8916-4 (eBook)
https://doi.org/10.1007/978-1-4939-8916-4
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Preface
Nanotechnologyisamongtheworld’smostpromisingnewtechnologies,anditisprojected
to hold significant importance in the twenty-first century. Nanotechnology has revolutio-
nized areas as diverse as engineering, information technology, and diagnostics. As the
prevalenceofnanomaterialscontinuestoincrease,theriskofenvironmentalcontamination
by nanoparticles is rising. Consequently, occupational and nonoccupational exposure to
nanoparticlesisgrowing,andtheirhealtheffectscannotbeignored.
Theuniquephysicalandchemicalpropertiesofsolidmaterialsatthenanoscalecontrib-
ute to serious technical limitations and potentially misleading results using conventional
toxicology assays. Due to their high surface area and hydrophobicity, nanoparticles can
adsorb vital dyes, cell culture micronutrients, or related cytokines; therefore, the routine
methods and protocols for larger-sized particles are unsuitable for nanotoxicology studies.
ThemethodsandprotocolsinNanotoxicity:MethodsandProtocolsofthesuccessfulMethods
in Molecular Biology series address the recently developed methods for investigating nano-
toxicity. These methods and protocols range from routine nanotoxicology assays to geno-
micsandproteomicstechniquesandincludebothinvitroandinvivoapproaches.Webelieve
thisbookcanserveasastartingpointtointroduceresearcherstothemethodsandprotocols
ofnanotoxicity,whichwillexpandtheworkbeingdoneinthisarea.
The target audience for this book comprises researchers and postgraduate students in
the fields of toxicology, environmental science, pharmacological science, and clinical
medicine.
I am deeply grateful to all contributing authors who devoted their valuable time and
effort in writing outstanding state-of-the-art methods and protocols for this book. I also
wouldliketothanktheserieseditor,JohnWalker,forhisvaluablesuggestions.
Louisville,KY,USA QunweiZhang
v
Contents
Preface ..................................................................... v
Contributors................................................................. ix
1 InVitroMethodsforAssessingNanoparticleToxicity....................... 1
DustinT.Savage,J.ZachHilt,andThomasD.Dziubla
2 MethodsforAssessingMastCellResponsestoEngineered
NanomaterialExposure.................................................. 31
NasserB.AlsalehandJaredM.Brown
3 PreparationandUtilizationofa3DHumanLiverMicrotissue
ModelforNanotoxicologicalAssessment .................................. 47
AliKermanizadeh
4 DerivingNeuralCellsfromPluripotentStemCells
forNanotoxicityTesting................................................. 57
YilingHong,NymphChan,andAynunN.Begum
5 MethodsforEvaluationofOvarianGranulosaCellswithExposure
toNanoparticles........................................................ 73
JinLiuandWenchangZhang
6 InVitroApproachesforAssessingtheGenotoxicityofNanomaterials ......... 83
MariaDusinska,EspenMariussen,EliseRunde´n-Pran,
AlexandraMisciHudecova,ElisabethElje,AlenaKazimirova,
NaoualeElYamani,NilsDommershausen,JulianTharmann,
DagmarFieblinger,FrankHerzberg,AndreasLuch,andAndreaHaase
7 MeasurementofNanoparticle-InducedMitochondrial
MembranePotentialAlterations .......................................... 123
SalikHussain
8 ApplicationofGelatinZymographyinNanotoxicityResearch ................ 133
YueZhang,RongWan,QunweiZhang,andYiqunMo
9 DeterminationofPhosphorylatedHistoneH2AX
inNanoparticle-InducedGenotoxicStudies................................ 145
RongWan,YiqunMo,RuiruiTong,MeiqinGao,andQunweiZhang
10 AnalysisofNanomaterialToxicitybyWesternBlot.......................... 161
GaoLong,YiqunMo,QunweiZhang,andMizuJiang
11 QuantificationofMitochondrialGenomeTranscription
inMaleReproductiveCellsbyReal-TimePCR............................. 171
ChengXu,QianLiu,JinXu,andAihuaGu
12 DNAMethylationAnalysis............................................... 181
LingfangFengandJianlinLou
13 EvaluationofNrf2withExposuretoNanoparticles......................... 229
FuliZhengandHuangyuanLi
14 ApplicationofElectronMicroscopesinNanotoxicityAssessment............. 247
JunzheZhang,XiaoHe,andMichaelT.Tseng
vii
viii Contents
15 MetabolicFingerprintingofBacteriaExposedtoNanomaterials,
UsingOnlineDatabases,NMR,andHigh-Resolution
MassSpectrometry...................................................... 271
TheodorosG.ChatzimitakosandConstantineD.Stalikas
16 MethodstoQuantifyNanomaterialAssociationwith,
andDistributionAcross,theBlood–BrainBarrierInVivo ................... 281
RobertA.Yokel
17 EvaluationofGenotoxicityofNanoparticlesinMouseModels ............... 301
SameeraNallanthighalandRamuneReliene
18 EvaluationofPulmonaryToxicityofNanoparticles
byBronchoalveolarLavage............................................... 313
YiqunMo,YueZhang,andQunweiZhang
19 PulmonaryFunctionTestinginAnimals................................... 323
GaryW.Hoyle,ConnieF.Schlueter,andSadiatuMusah
20 NanotoxicityAssessmentUsingEmbryonicZebrafish....................... 331
EduardDumitrescu,KennethWallace,andSilvanaAndreescu
21 ApplicationofDaphniamagnaforNanoecotoxicityStudy................... 345
ZhizhenXu,YingyingLiu,andYuqianWang
22 TheOverviewofMethodsofNanoparticleExposureAssessment............. 353
PengZhaoandYuanbaoZhang
Index ...................................................................... 369
Contributors
NASSERB.ALSALEH (cid:1) DepartmentofPharmaceuticalSciences,ColoradoCenter for
NanomedicineandNanosafety,SkaggsSchoolofPharmacyandPharmaceuticalSciences,
UniversityofColorado,Aurora,CO,USA
SILVANAANDREESCU (cid:1) DepartmentofChemistryandBiomolecularScience,Clarkson
University,Potsdam,NY,USA
AYNUNN.BEGUM (cid:1) CollegeofVeterinaryMedicine,WesternUniversityofHealthSciences,
Pomona,CA,USA
JAREDM.BROWN (cid:1) DepartmentofPharmaceuticalSciences,ColoradoCenter for
NanomedicineandNanosafety,SkaggsSchoolofPharmacyandPharmaceuticalSciences,
UniversityofColorado,Aurora,CO,USA
NYMPHCHAN (cid:1) CollegeofVeterinaryMedicine,WesternUniversityofHealthSciences,
Pomona,CA,USA
THEODOROSG.CHATZIMITAKOS (cid:1) DepartmentofChemistry,UniversityofIoannina,
Ioannina,Greece
NILSDOMMERSHAUSEN (cid:1) DepartmentofChemicalandProductSafety,GermanFederal
InstituteforRiskAssessment(BfR),Berlin,Germany
EDUARDDUMITRESCU (cid:1) DepartmentofChemistryandBiomolecularScience,Clarkson
University,Potsdam,NY,USA
MARIADUSINSKA (cid:1) HealthEffectsLaboratory,DepartmentofEnvironmentalChemistry,
NorwegianInstituteforAirResearch(NILU),Kjeller,Norway
THOMASD.DZIUBLA (cid:1) DepartmentofChemicalandMaterialsEngineering,Universityof
Kentucky,Lexington,KY,USA
NAOUALEELYAMANI (cid:1) HealthEffectsLaboratory,DepartmentofEnvironmentalChemistry,
NorwegianInstituteforAirResearch(NILU),Kjeller,Norway
ELISABETHELJE (cid:1) HealthEffectsLaboratory,DepartmentofEnvironmentalChemistry,
NorwegianInstituteforAirResearch(NILU),Kjeller,Norway
LINGFANGFENG (cid:1) InstituteofOccupationalDiseases,ZhejiangAcademyofMedicalSciences,
Hangzhou,P.R.China
DAGMARFIEBLINGER (cid:1) DepartmentofChemicalandProductSafety,GermanFederal
InstituteforRiskAssessment(BfR),Berlin,Germany
MEIQIN GAO (cid:1) DepartmentofPathology,SchoolofBasicMedicalSciences,FujianMedical
University,Fuzhou,People’sRepublicofChina
AIHUAGU (cid:1) StateKeyLaboratoryofReproductiveMedicine,InstituteofToxicology,Nanjing
MedicalUniversity,Nanjing,China;KeyLaboratoryofModern ToxicologyofMinistryof
Education,SchoolofPublicHealth,NanjingMedicalUniversity,Nanjing,China
ANDREAHAASE (cid:1) DepartmentofChemicalandProductSafety,GermanFederalInstitutefor
RiskAssessment(BfR),Berlin,Germany
XIAOHE (cid:1) CASKeyLabforBiomedicalEffectsofNanomaterialsandNanosafety,Institute
ofHighEnergyPhysics,ChineseAcademyofSciences,Beijing,P.R.China
FRANKHERZBERG (cid:1) DepartmentofChemicalandProductSafety,GermanFederalInstitute
forRiskAssessment(BfR),Berlin,Germany
J.ZACHHILT (cid:1) DepartmentofChemicalandMaterialsEngineering,Universityof
Kentucky,Lexington,KY,USA
ix
x Contributors
YILINGHONG (cid:1) CollegeofVeterinaryMedicine,WesternUniversityofHealthSciences,
Pomona,CA,USA
GARYW.HOYLE (cid:1) DepartmentofEnvironmentalandOccupationalHealthSciences,School
ofPublicHealthandInformationSciences,UniversityofLouisville,Louisville,KY,USA
ALEXANDRAMISCIHUDECOVA (cid:1) HealthEffectsLaboratory,DepartmentofEnvironmental
Chemistry,NorwegianInstituteforAirResearch(NILU),Kjeller,Norway
SALIKHUSSAIN (cid:1) DepartmentofPhysiologyandPharmacology,RobertC.ByrdHealthScience
Center,SchoolofMedicine,WestVirginiaUniversity,Morgantown,WV,USA
MIZUJIANG (cid:1) DepartmentofGastroenterology,AffiliatedChildren’sHospital,Zhejiang
UniversitySchoolofMedicine,Hangzhou,ZhejiangProvince,China
ALENAKAZIMIROVA (cid:1) HealthEffectsLaboratory,DepartmentofEnvironmentalChemistry,
NorwegianInstituteforAirResearch(NILU),Kjeller,Norway
ALIKERMANIZADEH (cid:1) NanoSafetyResearchGroup,SchoolofEngineeringandPhysical
Science,InstituteofBiologicalChemistry,BiophysicsandBioengineering,Heriot-Watt
University,Edinburgh,UK
HUANGYUAN LI (cid:1) FujianProvincialKeyLaboratoryofEnvironmentalFactorsandCancer,
SchoolofPublicHealth,FujianMedicalUniversity,Fuzhou,P.R.China;Departmentof
PreventiveMedicine,SchoolofPublicHealth,FujianMedicalUniversity,Fuzhou,P.R.
China
JINLIU (cid:1) FujianProvincialKeyLaboratoryofEnvironmentalFactorsandCancer,Schoolof
PublicHealth,FujianMedicalUniversity,Fuzhou,People’sRepublicofChina;
DepartmentofPreventiveMedicine,SchoolofPublicHealth,FujianMedicalUniversity,
Fuzhou,People’sRepublicofChina
QIANLIU (cid:1) StateKeyLaboratoryofReproductiveMedicine,InstituteofToxicology,Nanjing
MedicalUniversity,Nanjing,China;KeyLaboratoryofModern ToxicologyofMinistryof
Education,SchoolofPublicHealth,NanjingMedicalUniversity,Nanjing,China
YINGYINGLIU (cid:1) DepartmentofRawMaterialIndustryResearch,ChinaCenter for
InformationIndustryDevelopment,Beijing,People’sRepublicofChina
GAOLONG (cid:1) DepartmentofGastroenterology,AffiliatedChildren’sHospital,Zhejiang
UniversitySchoolofMedicine,Hangzhou,ZhejiangProvince,China
JIANLINLOU (cid:1) InstituteofOccupationalDiseases,ZhejiangAcademyofMedicalSciences,
Hangzhou,P.R.China
ANDREASLUCH (cid:1) DepartmentofChemicalandProductSafety,GermanFederalInstitutefor
RiskAssessment(BfR),Berlin,Germany
ESPEN MARIUSSEN (cid:1) HealthEffectsLaboratory,DepartmentofEnvironmentalChemistry,
NorwegianInstituteforAirResearch(NILU),Kjeller,Norway
YIQUN MO (cid:1) DepartmentofEnvironmentalandOccupationalHealthSciences,Schoolof
PublicHealthandInformationSciences,UniversityofLouisville,Louisville,KY,USA
SADIATUMUSAH (cid:1) DepartmentofEnvironmentalandOccupationalHealthSciences,School
ofPublicHealthandInformationSciences,UniversityofLouisville,Louisville,KY,USA
SAMEERANALLANTHIGHAL (cid:1) CancerResearchCenter,UniversityatAlbany,StateUniversity
ofNewYork,Rensselaer,NY,USA;DepartmentofBiomedicalSciences,Universityat
Albany,StateUniversityofNewYork,Albany,NY,USA
RAMUNE RELIENE (cid:1) CancerResearchCenter,UniversityatAlbany,StateUniversityofNew
York,Rensselaer,NY,USA;DepartmentofEnvironmentalHealthSciences,Universityat
Albany,StateUniversityofNewYork,Albany,NY,USA
Contributors xi
ELISERUNDE´N-PRAN (cid:1) HealthEffectsLaboratory,DepartmentofEnvironmentalChemistry,
NorwegianInstituteforAirResearch(NILU),Kjeller,Norway
DUSTIN T.SAVAGE (cid:1) DepartmentofChemicalandMaterialsEngineering,Universityof
Kentucky,Lexington,KY,USA
CONNIEF.SCHLUETER (cid:1) DepartmentofEnvironmentalandOccupationalHealthSciences,
SchoolofPublicHealthandInformationSciences,UniversityofLouisville,Louisville,KY,
USA
CONSTANTINED.STALIKAS (cid:1) DepartmentofChemistry,UniversityofIoannina,Ioannina,
Greece
JULIANTHARMANN (cid:1) DepartmentofChemicalandProductSafety,GermanFederalInstitute
forRiskAssessment(BfR),Berlin,Germany
RUIRUI TONG (cid:1) DepartmentofPathology,SchoolofBasicMedicalSciences,FujianMedical
University,Fuzhou,People’sRepublicofChina
MICHAELT.TSENG (cid:1) DepartmentofAnatomicalSciencesandNeurobiology,Universityof
Louisville,Louisville,KY,USA
KENNETHWALLACE (cid:1) DepartmentofBiology,ClarksonUniversity,Potsdam,NY,USA
RONGWAN (cid:1) DepartmentofPathology,SchoolofBasicMedicalSciences,FujianMedical
University,Fuzhou,People’sRepublicofChina;DepartmentofEnvironmentaland
OccupationalHealthSciences,SchoolofPublicHealthandInformationSciences,
UniversityofLouisville,Louisville,KY,USA
YUQIANWANG (cid:1) KeyLaboratoryofOccupationalSafetyandHealth,BeijingMunicipal
InstituteofLabourProtection,Beijing,People’sRepublicofChina
CHENGXU (cid:1) StateKeyLaboratoryofReproductiveMedicine,InstituteofToxicology,Nanjing
MedicalUniversity,Nanjing,China;KeyLaboratoryofModern ToxicologyofMinistryof
Education,SchoolofPublicHealth,NanjingMedicalUniversity,Nanjing,China;
DepartmentofCardiothoracicSurgery,Children’sHospitalofNanjingMedical
University,Nanjing,China
JINXU (cid:1) StateKeyLaboratoryofReproductiveMedicine,InstituteofToxicology,Nanjing
MedicalUniversity,Nanjing,China;KeyLaboratoryofModern ToxicologyofMinistryof
Education,SchoolofPublicHealth,NanjingMedicalUniversity,Nanjing,China
ZHIZHEN XU (cid:1) KeyLaboratoryofOccupationalSafetyandHealth,BeijingMunicipal
InstituteofLabourProtection,Beijing,People’sRepublicofChina
ROBERTA.YOKEL (cid:1) PharmaceuticalSciencesDepartment,UniversityofKentuckyAcademic
MedicalCenter,Lexington,KY,USA
JUNZHEZHANG (cid:1) CASKeyLabforBiomedicalEffectsofNanomaterialsandNanosafety,
InstituteofHighEnergyPhysics,ChineseAcademyofSciences,Beijing,P.R.China
QUNWEI ZHANG (cid:1) DepartmentofEnvironmentalandOccupationalHealthSciences,School
ofPublicHealthandInformationSciences,UniversityofLouisville,Louisville,KY,USA
WENCHANGZHANG (cid:1) FujianProvincialKeyLaboratoryofEnvironmentalFactorsand
Cancer,SchoolofPublicHealth,FujianMedicalUniversity,Fuzhou,People’sRepublicof
China;DepartmentofPreventiveMedicine,SchoolofPublicHealth,FujianMedical
University,Fuzhou,People’sRepublicofChina
YUANBAOZHANG (cid:1) KeyLaboratoryofOccupationalSafetyandHealth,BeijingMunicipal
InstituteofLabourProtection,Beijing,People’sRepublicofChina
YUEZHANG (cid:1) DepartmentofEnvironmentalandOccupationalHealthSciences,Schoolof
PublicHealthandInformationSciences,UniversityofLouisville,Louisville,KY,USA