Mullin et al, PlOS One 2010 study on miticides and agrochemicals in beehives, pollen and wax

19 Pages·2015·0.45 MB·English

High Levels of Miticides and Agrochemicals in North
American Apiaries: Implications for Honey Bee Health
Christopher A. Mullin1*, Maryann Frazier1, James L. Frazier1, Sara Ashcraft1, Roger Simonds2, Dennis
vanEngelsdorp3, Jeffery S. Pettis4
1DepartmentofEntomology,ThePennsylvaniaStateUniversity,UniversityPark,Pennsylvania,UnitedStatesofAmerica,2NationalScienceLaboratory,UnitedStates
DepartmentofAgriculture-AgriculturalMarketingService,Gastonia,NorthCarolina,UnitedStatesofAmerica,3PennsylvaniaDepartmentofAgriculture,Harrisburg,
Pennsylvania,UnitedStatesofAmerica,4BeeResearchLaboratory,UnitedStatesDepartmentofAgriculture-AgriculturalResearchService,Beltsville,Maryland,United
StatesofAmerica
Abstract
Background:Recentdeclinesinhoneybeesforcroppollinationthreatenfruit,nut,vegetableandseedproductioninthe
UnitedStates.Abroadsurveyofpesticideresidueswasconductedonsamplesfrommigratoryandotherbeekeepersacross
23states, one Canadianprovinceand severalagricultural croppingsystems during the 2007–08 growingseasons.
Methodology/PrincipalFindings:We have used LC/MS-MS and GC/MS to analyze bees and hive matrices for pesticide
residuesutilizingamodifiedQuEChERSmethod.Wehavefound121differentpesticidesandmetaboliteswithin887wax,
pollen, bee and associated hive samples. Almost 60% of the 259 wax and 350 pollen samples contained at least one
systemicpesticide,andover47%hadbothin-hiveacaricidesfluvalinateandcoumaphos,andchlorothalonil,awidely-used
fungicide.Inbeepollenwerefoundchlorothalonilatlevelsupto99 ppmandtheinsecticidesaldicarb,carbaryl,chlorpyrifos
and imidacloprid, fungicides boscalid, captan and myclobutanil, and herbicide pendimethalin at 1ppm levels. Almost all
combandfoundationwaxsamples(98%)werecontaminatedwithupto204and94ppm,respectively,offluvalinateand
coumaphos, and lower amounts of amitraz degradates and chlorothalonil, with an average of 6 pesticide detections per
sampleandahighof39.Therewerefewerpesticidesfoundinadultsandbroodexceptforthoselinkedwithbeekillsby
permethrin (20ppm) andfipronil (3.1 ppm).
Conclusions/Significance:The 98 pesticides and metabolites detected in mixtures up to 214ppm in bee pollen alone
representsaremarkablyhighlevelfortoxicantsinthebroodandadultfoodofthisprimarypollinator.Thisrepresentsover
half of the maximum individual pesticide incidences ever reported for apiaries. While exposure to many of these
neurotoxicants elicits acute and sublethal reductions in honey bee fitness, the effects of these materials in combinations
and theirdirectassociation with CCDordeclining beehealth remainsto bedetermined.
Citation:MullinCA,FrazierM,FrazierJL,AshcraftS,SimondsR,etal.(2010)HighLevelsofMiticidesandAgrochemicalsinNorthAmericanApiaries:Implications
forHoneyBeeHealth.PLoSONE5(3):e9754.doi:10.1371/journal.pone.0009754
Editor:FredericMarion-Poll,INRA-Paris6-AgroParisTech,France
ReceivedDecember22,2009;AcceptedFebruary26,2010;PublishedMarch19,2010
Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsPublicDomaindeclarationwhichstipulatesthat,onceplacedinthepublic
domain,thisworkmaybefreelyreproduced,distributed,transmitted,modified,builtupon,orotherwiseusedbyanyoneforanylawfulpurpose.
Funding:FundingwasreceivedfromtheFloridaStateBeekeepers,NationalHoneyBoard,PennStateCollegeofAgricultureSciences,ProjectApismellifera
(PAm),TampaBayBeekeepers,TheFoundationforthePreservationofHoneyBees,andtheUnitedStatesDepartmentofAgricultureCriticalIssuesprogram.The
fundershadnoroleinstudydesign,datacollectionandanalysis,decisiontopublish,orpreparationofthemanuscript.
CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist.
*E-mail:[email protected]
Introduction like pyrethroids, organophosphates and associated fungicides and
herbicides that can be monitored through conventional gas
OnethirdofhoneybeecoloniesintheUSwerelostduringeachof chromatography-mass spectrometry (GC-MS). High-value seed
the last three winters between ’06-’09 [1–3]. This alarming technologieshavedrivengreaterdeploymentofsystemicpesticides
overwinter along with other losses of this primary pollinator, Apis to seasonally protect all plant organs including flowers, which
melliferaL.,aswellasthoseofnativepollinators,hasbeendocumented inadvertently contaminates pollen and nectar. Themore recently
inNorthAmericaandEurope[4,5].Themostrecentmanifestation developed liquid chromatography-tandem mass spectrometry
of this decline, Colony Collapse Disorder (CCD), has led to a (LC/MS-MS) analytical capability is essential for monitoring
significant collaborative effortinvolving several land grant universi- systemic insecticides, like neonicotinoids [7,8]. The enhanced
ties,DepartmentsofAgricultureandtheUSDA.Overthepasttwo sensitivity provided by LC/MS-MS allows measurement of
years, the CCD working team has been investigating the possible residues at the ppb level known to affect bees sublethally, not
cause(s)responsibleforCCD.CCDischaracterizedbyarapidlossof killing them outright, but rather impairing behaviors or immune
adultbees,butnotthequeenandbrood,alongwiththeabsenceof responses [9–11]. Other systemics such as aldicarb and its toxic
invasiveresponsesbyrobberbeesandotherhivepests[1]. metabolites, and numerous polar pesticides and their degradates
Pesticides have long been suspected as a potential cause of could not be analyzed at ppb limits of detection without LC-MS
honeybeedeclines[5,6].Manyofthesearelipophiliccompounds technology [12,13].
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Since 1999, beekeepers in France experiencing bee losses pesticides exposure to honey bee colonies engaged in apple
described as ‘‘mad bee disease’’ have blamed the systemic pollination, samples of pollen, wax and bees were collected from
neonicotinoid pesticide,imidacloprid [14].Labstudies confirmed 47coloniesin2007and2008.Thesecoloniesweredistributedin
itstoxicitytobees,includingimpairedlearningandmemory[10], three Pennsylvania apple orchards with known pesticide applica-
and field studies found low levels of imidacloprid in a high tionhistoriesandacontrollocation.In2007,alongitudinalstudy
percentageofpollensamplescollectedfrommaize,sunflowerand wasconductedwhichfollowedtaggedcoloniesinthreemigratory
canola [7,8]. Conflicting data exist for establishing a causal operationsastheymovedfromFloridaupanddowntheeastcoast
relationship between imidacloprid and honey bee losses, yet to pollinate a variety of crops (citrus, high bush blueberries, low
regulatory concernsremain [15]. bush blueberries, apples, cucumbers, squash, pumpkin). Samples
The interactions between pesticides [16], mite stresses and ofpollen,wax,andadultbees,anddetailedcolonymeasurements
diseasesincludingthenewlyidentifiedIsraeliacuteparalysisvirus weretakeneachtimethesecoloniesweremovedtoanewcrop.In
[IAPV, 17] are likely contributing factors, and support an this last survey [24] a new phenomenon, entombed and capped
emerging hypothesis that no one factor alone is responsible for pollen,wasobserved,andsamplesofthesepollens,plusrespective
the dramatic losses of honey bees in general or for CCD wax, were included here. In these three studies, samples were
specifically[18].Onlythemiticidecoumaphoswasatconsistently collected by researchers from the CCD working group. In 2008,
higherlevelsinnon-CCDversusCCDcoloniesoutof50pesticides 65ofthepollen,wax,adultbeeandhoneysamplesweresubmitted
and metabolites found [18], supporting its beneficial role in foranalysisdirectlybybeekeepersfrom13differentstatesaspart
promotingapiaryhealthbyreducingVarroamitestress.Pesticides of a programtosharethecost ofanalysis.
havebeenimplicatedinthedeclinesofotherbioindicatorspecies Insomecasessampledcolonieshada,15cmby10cmsection
includingthealteringofolfactorybehaviorinwesternUSsalmon ofbroodcombremovedandwrappedinaluminumfoilandstored
[19], disrupting signaling required for recruitment of nitrogen- ondryiceuntilplacedina280uCfreezer.Thesesectionsofcomb
fixingbacterialsymbionts[20],andcausingendocrinedisruption, contained beeswax, beebread and brood. Beebread and brood
increaseddiseasesusceptibility,andpotentialdeclinesinfrogsand were removed from the combs at room temperature and then
other amphibian species through synergistic interactions with stored along with the remaining beeswax at 220uC until
chytrid fungi [21,22]. A potential involvement of pesticides processing. In other cases, samples of beebread collected in the
remainstobeinvestigatedinelicitingthe‘‘white-nosesyndrome’’ fieldwereremovedfromthebroodnestofcoloniesusingaspatula
that isdecimating northeastern USbatpopulations [23]. cleaned using CloroxH wipes and rinsed with 75% ethanol
During 2007 to 2008, we actively sampled beebread, trapped between collections. Beebread was placed in a 1.5ml Eppendorf
pollen, brood nest wax, beeswax foundation, and adult bees and tubeondryiceuntilstorageat220uC.Samplesofbroodnestwax
brood for pesticide residues. These samples were drawn largely collected in the field were scraped with a sterilized standard hive
from commercial beekeepers from several states and one toolintoa50mlcentrifugetubeandsimilarlystored.Whilecare
Canadianprovince,andincludedsamplesfromapparentlyhealthy was taken to sample sections of the comb without honey, nectar,
coloniesaswellasfromoperationsthatwerediagnosedashaving beebread or brood, small levels of cross-contamination were
CCD.Includedinthissurveyweredeadbeescollectedfromlocal inevitable. Adult nurse bees were removed from the brood nest
or community applications of insecticides. A comprehensive and andplacedinto50mlcentrifugetubesondryiceuntiltheycould
sensitiveanalyticalsurveyof200miticides,insecticides,fungicides bestored at 280uC.
andherbicideswasconducted,includingsomenolongerregistered In the apple orchard study, samples were field collected as
foruse,tobroadlyassessknownbeetoxicantsandotherlikelyco- described above but were placed on ice after collection and then
occurringpesticides.Herewedocumenttheplethoraofpesticides stored in a standard freezer (220uC). Beekeepers submitting
that are currently present in US beehives and discuss their sampleswereprovidedwithastandardizedprotocolforcollecting,
potential risks tohoney beehealth. storing and shipping samples. They were instructed to freeze all
samples as soon as possible after collectionandthen ship samples
Materials and Methods overnight or second-day delivery in insulated containers with ice
packs.Uponarrivalthesesampleswerestoredinastandardfreezer.
Beehive samples Foundationisprocessedbeeswaxpressedintosheetsandusedas
In2007and2008weanalyzedpollen(totalof320beebread,28 templates for uniform comb construction. Wax samples from six
trapped pollen, and 2 anther samples), 238 wax (derived mainly differentcommercialandtwoprivatesourceswereanalyzed.This
from the brood nest of colonies) and 21 foundation samples, and included onesample ofwax fromwax-coated plastic foundation.
34 immature (brood) and 106 adult bee samples for pesticide Themajorityofsamples(749)weanalyzedincludedbroodnest
residues.Thesesampleswerecollectedaspartofdifferentstudies waxandfoundation,pollenandbeesfromcoloniesassociatedwith
and epidemiological surveys to investigate possible threats to thespecificresearchprojectsdescribedabove.Whilethesampling
colony health. The studies and surveys are described here. In wasnotcompletelyrandomacrosstimeandspace,itdoesinclude
January and February 2007, colonies resident in Florida and migratory or stationary colonies diagnosed as having ‘‘CCD’’ as
California distributed across 13 apiaries owned by 11 different well as those diagnosed as healthy, colonies placed in orchards
beekeepers were selected to participate in multi-factorial study. withknownpesticideapplicationhistoryaswellascontrolcolonies
Apiaries were classified as 1) having no colonies with CCD notplacedinorchards,andsamplessubmittedbybeekeeperfrom
symptoms (‘control’) or 2) having colonies with CCD symptoms coloniesdescribedas‘‘unhealthy’’aswellasfromthoseidentified
(‘CCD’).ColonieswereconsideredtohaveCCDsymptomswhen as‘‘healthy.’’Theresultsandconclusionreportedherearedrawn
adult bee populations were in obvious decline leaving brood mainlyfromthesedata.Inaddition,weanalyzed158samplesthat
poorlyattended,orweredeadinanapiaryhavingclearsymptoms includedmixedmatrices(pollenandwax),Osmia-collectedpollen,
ofCCD.InthoseCCDcolonieswherebeesremained,therewere floralnectar,hivesupplements(cornsyrup,pollensubstitute),royal
insufficient number of bees to cover the brood, the remaining jelly, honey, samples obtained outside of U.S. and Canada and
worker bees appeared young (i.e., adults bees that are unable to irradiatedsamples.Residuedataonthesesamplesareincludedin
fly), and the queen was present. In a second study to investigate TableS1.
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Multiresidue pesticide analysis Bee toxicity
Samplesovertheentirestudywereanalyzedfor200chemicals Honey bee LD values are averaged 24–72h adult acute
50
atanaverageof171pesticidesandtoxicmetabolitesperanalysis. toxicities from the EPA-OPP Pesticide Ecotoxicity Database
New compounds were added and others removed depending on [http://www.ipmcenters.org/Ecotox/DataAccess.cfm] and pri-
lackofdetectionornegligiblefrequencyofusewherebeesforage. mary literature [27–29]. Standard LD50 values in terms of mg/
PesticideresidueanalysiswasconductedbytheUSDA-AMS-NSL beewereconvertedtoppbrelativetobodyweight(ngpesticideper
at Gastonia, NC. For multi-residue pesticide analysis, a modified g bee) by multiplying using a factor of 10,000; equivalent
QuEChERS method was used [25] that was adapted for 3 g to1000ng per mg 4 averagebee weightof 0.1g.
insteadofthenormal15gsamples.Beebreadorcombwax(3g)is
weighed into a 50ml plastic centrifuge tube and fortified with Statistical analyses
100mloftheprocesscontrolspiking(PCS)solution.Afteradding Mean, medians, percentiles, and standard errors of the means
27ml of extraction solution (44% deionized water, 55%
for individual pesticides and metabolites for all matrix-specific or
acetonitrile, and 1% glacial acetic acid), each sample is then
pairedpesticideanalyseswerecalculatedusing0 ppbforanynon-
fortified with 100ml of the internal standard (ISTD) spiking detection (N.D.), unless otherwise noted. In-hive and between
solution.Forbeebread,theparticlesizeisreducedbyusingahigh
colony comparisons of pesticide detections were made by pairing
speed disperser for approximately 1 minute. For comb wax, the
749 bee, pollen and wax sample analyses by colony/matrix, and
sampleismeltedanddispersedbyheatingto80uCfor20minina
then sorting colonies for concurrently-sampled matrices. This
water bath, followed by cooling to room temperature. To each
paireddatabaseof519analyseswasfurtheraveragedaccordingto
sample is then added 6 g of anhydrous magnesium sulfate
matrix by colony identity if sampling dates were not identical.
(MgSO ) and1.5g anhydrous sodium acetate (NaAc).Tubes are
4 Significanttrendswereextractedbycorrelationfollowedbylinear
sealedandshakenvigorouslyfor1minute,centrifuged,and1 mlof
regression analysis of these data using Microsoft Excel Data
supernatant A or its concentrate transferred to a 2 ml mini-
Analysis package (ver. 11.5) or SAS JMP ver. 9.0. A two sample
centrifuge tube that contains 0.05 g primary secondary amine
one way ANOVA was used to determine significant differences
(PSA), 0.05g C18, and 0.15g MgSO (United Chemical
4 between compounds ortreatments at theP,0.05level.
Technologies, Lewistown, PA). After vortexing for 1 minute and
centrifugation, the resulting supernatant is transferred to an
Results
autosampler vial for analysis by LC/MS-MS using a 3.5mm,
2.16150mm Agilent Zorbax SB-C18 column and an Agilent Honey bees across North America are extensively
1100LCwithabinarypumpinterfacedtoaThermo-FisherTSQ
exposed to multiple pesticides
Quantum Discovery triplequadrupole MS.
Broodnestwaxandfoundations,beebreadandtrappedpollen,
For GC analyses, a dual layer solid-phase extraction (SPE)
andadultbeesandbroodcomprising749samplescontained118
cartridgecontaining250mgofgraphitizedcarbonblack(GCB)and
different pesticides and metabolites, 4894 total residues of which
500mgofPSAispreparedwithapproximately0.80gofanhydrous
748weresystemics,andaveraged6.5detectionspersample.Inthe
MgSO added to the top of the cartridge. After conditioning the
4 259 wax samples (Table 1) 87 pesticides and metabolites were
SPEcartridgebyaddingonecartridgevolume(4.0ml)ofacetone/
found with up to 39 different detections in a single sample,
toluene(7:3;v/v)usingapositivepressureSPEmanifoldandeluting
averaging 8 different pesticide residues each. In the 350 pollen
towaste,2mlofsupernatantA(above)isappliedtothecartridge.
samples analyzed (Table 2), 98 pesticides and degradates were
Pesticideanalytesareelutedwith3by4mlofacetone/toluene(7:3;
identified, with up to 31 different pesticides found in a single
v/v)intoa15mlgraduatedglasscentrifugetube.UsinganN-Evap
sample,andsamplesaveraged7.1differentpesticideresidueseach.
at50uC,eluatesaredriedusingtolueneandconcentratedtoafinal
The analysis of bees resulted in fewer detections (Table 3), and
volumeof0.4mlforanalysisusingGC/MSintheelectronimpact
and negative chemical ionization modes. An Agilent 6890 GC averaged 2.5 residues per each of the 140 samples, with a
equipped with a 0.25mm id630m J&W DB-5MS (2mm film) maximumof25inonesample.Onlyoneofthewax,threepollen
capillary column interfaced to an Agilent 5975 triple quadrupole and12bee sampleshadno detectable pesticides.
MSwasused.Aparallelmethodwasusedforthebroodandadult Multipleresiduesprevailedinthebee,pollenandwaxsamples,
bee matrices, except that water was deleted from the extraction with 2 or more pesticides detected in 92.3% of 749 analyzed
solutionduetoitshighcontentinthesamples. (Table4).Almosthalfofthesesamples(49.9%)containedatleast
Extracts of wax, beebread, and adult bees or brood were also onesystemicpesticide.Themostfrequentbinarypairofdetections
analyzedforpotentiallytoxicmetabolitesofprimarymiticideand were the miticides fluvalinate and coumaphos found in 77.7% of
insecticide detections. This included the respective oxon and the samples,followedbythepyrethroidfluvalinatewiththefungicide
phenolicmetaboliteofcoumaphos,chlorferone,thesulfoxideand chlorothalonil (41.2%), fluvalinate with the organophosphate
sulfonemetabolitesofaldicarb,andthetoxicolefinand5-hydroxy chlorpyrifos (39.4%), and the organophosphate coumaphos with
metabolites of imidacloprid. Pesticides and metabolites were chlorothalonil(39.1%).All393bee,pollenorwaxsampleswitha
obtainedinhigh-purityasstandardsfromtheEPA,ChemService fungicide detection (52.5%), except 9, had at least one other
(West Chester, PA), or the manufacturer at the highest purity pyrethroid or organophosphate insecticide/miticide present. The
available. most prevalent ternary combinations contained fluvalinate and
Identity of parent pesticides and metabolites from extracts was coumaphos with chlorothalonil (38.6% of samples analyzed),
based on co-chromatography with known standards by GC/MS chlorpyrifos(34.4%)ordegradatesofthemiticideamitraz(32.6%).
and/or LC/MS-MS and consistent ratios of parent mass At least one each of an insecticide/acaricide, fungicide or
abundance to at least two fragment transitions. Standard parent herbicidewerefoundin28.5%ofsamples.Thehighestfrequency
mass and fragment ion transitions used [12] are also available ofquaternarycombinationsofpesticideswerethethreemiticides,
online[26].Amatrix-dependentlimitofdetection(LOD)foreach fluvalinate,coumaphosandamitraz,withchlorothalonil(24%)or
parent and metabolite was determined after adjustment for chlorpyrifos(15.7%)orfluvalinate,coumaphos,chlorothaloniland
recovery of theISTD. chlorpyrifos (19.2%).
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Table1. Summaryof pesticide detectionsinwax samplesfrom North American honey beecolonies.
WaxPesticide* Class# Detects Samples % Detections(ppb)
Analyzed High Low Median 90%tile 95%tile Mean1 SEM1 LOD{
Fluvalinate PYR 254 259 98.1 204000.0 2.0 3595.0 15080.0 28710.5 7473.8 973.6 1.0
Coumaphos OP 254 259 98.1 91900.0 1.0 1240.0 6875.0 11340.0 3300.4 499.8 1.0
Coumaphosoxon OP 187 208 89.9 1300.0 1.3 56.1 184.2 269.8 102.7 12.5 5.0
Chlorpyrifos OP 163 258 63.2 890.0 1.0 4.3 28.5 55.7 24.5 7.5 0.1
Chlorothalonil FUNG 127 258 49.2 53700.0 1.0 91.4 1552.0 2623.0 1066.6 453.4 1.0
DMPF(amitraz) FORM 107 177 60.5 43000.0 9.2 228.0 4718.0 8093.0 2199.8 574.2 4.0
EndosulfanI CYC 97 258 37.6 95.0 1.2 4.1 13.0 31.0 8.7 1.5 0.1
EndosulfanII CYC 65 258 25.2 39.0 1.1 3.8 10.9 21.2 6.2 0.8 0.1
DMA(amitraz) FORM 60 177 33.9 3820.0 120.0 437.0 1664.0 2433.0 742.1 104.6 50.0
Pendimethalin HERB 49 176 27.8 84.0 2.5 6.1 18.7 36.0 10.9 2.1 1.0
Fenpropathrin PYR 44 258 17.1 200.0 1.3 14.3 51.3 61.3 24.8 5.0 0.4
Esfenvalerate PYR 43 258 16.7 56.1 1.0 4.5 17.0 19.9 8.9 1.5 0.5
Azoxystrobin SFUNG 40 258 15.5 278.0 1.0 5.7 22.4 40.4 15.4 6.9 1.0
Methoxyfenozide IGR 39 208 18.8 495.0 3.5 42.3 171.0 271.4 81.5 17.2 0.4
Bifenthrin PYR 33 258 12.8 56.1 1.5 5.3 18.5 39.5 9.8 2.3 0.4
Endosulfansulfate CYC 29 258 11.2 33.0 1.3 3.0 12.1 18.4 6.3 1.3 0.1
Atrazine SHERB 29 208 13.9 31.0 1.0 5.5 16.5 18.4 8.2 1.3 1.0
Dicofol OC 26 258 10.1 21.0 1.5 5.1 15.1 17.5 6.8 1.1 0.4
Aldicarbsulfoxide SCARB 22 208 10.6 649.0 13.4 298.5 609.2 638.8 306.6 48.0 20.0
Trifluralin HERB 22 176 12.5 36.0 1.0 1.4 2.2 21.0 3.9 1.8 1.0
Boscalid SFUNG 21 208 10.1 388.0 16.9 84.0 261.0 265.0 109.8 20.6 1.0
Carbendazim SFUNG 21 208 10.1 133.0 2.1 12.0 48.7 87.0 23.2 7.0 1.0
Oxyfluorfen HERB 16 258 6.2 34.0 2.1 6.1 26.5 29.1 11.1 2.6 0.5
Methidathion OP 15 258 5.8 78.7 2.9 10.0 23.0 40.5 15.3 4.8 1.0
Aldicarbsulfone SCARB 15 208 7.2 49.6 18.0 27.5 45.8 48.1 31.0 2.8 10.0
Iprodione FUNG 14 208 6.7 636.0 32.6 164.5 555.2 586.6 269.7 52.4 10.0
Pyrethrins PYR 13 208 6.3 222.0 19.0 78.7 151.4 181.2 84.5 16.9 20.0
Cypermethrin PYR 13 258 5.0 131.0 4.5 13.2 95.3 114.8 31.2 11.4 1.0
Norflurazon SHERB 13 208 6.3 38.1 1.1 2.9 5.6 18.7 5.8 2.7 1.0
Vinclozolin FUNG 13 258 5.0 27.0 1.2 4.6 21.7 24.6 8.8 2.4 1.0
Cyhalothrin PYR 13 258 5.0 16.9 1.0 5.7 13.2 15.3 6.5 1.3 0.1
Chlorferone(coumaphos) OP 11 176 6.3 4390.0 299.0 932.0 2830.0 3610.0 1236.7 381.6 25.0
Cyprodinil SFUNG 11 208 5.3 106.0 6.2 17.0 85.4 95.7 34.7 10.3 5.0
Cyfluthrin PYR 11 258 4.3 44.7 3.2 7.8 17.0 30.9 12.6 3.5 1.0
Pyraclostrobin FUNG 10 208 4.8 438.0 1.8 27.3 193.2 315.6 84.2 42.4 1.0
Fenbuconazole SFUNG 10 176 5.7 183.0 7.4 46.1 86.0 134.5 54.2 15.7 6.0
Tebufenozide IGR 10 208 4.8 27.7 2.0 5.3 18.3 23.0 8.0 2.6 2.0
Pronamide SHERB 10 208 4.8 22.8 1.7 3.0 12.5 17.6 6.1 2.1 1.0
Deltamethrin PYR 8 258 3.1 613.0 107.0 129.5 368.0 490.5 209.9 60.6 20.0
Allethrin PYR 8 208 3.8 139.0 1.7 9.2 62.1 100.5 28.0 16.1 1.0
Trifloxystrobin PSFUNG 8 258 3.1 22.4 2.6 4.2 12.0 17.2 6.7 2.3 0.5
Azinphosmethyl OP 6 258 2.3 121.0 10.9 18.8 75.0 98.0 35.2 17.4 3.0
Tribufos = DEF SYN 6 208 2.9 59.0 7.6 19.3 44.1 51.5 25.1 7.4 2.0
Malathion OP 6 258 2.3 35.1 4.0 5.2 26.6 30.8 12.1 5.1 1.0
p-Dichlorobenzene OC 5 130 3.8 1050.0 6.9 30.9 642.7 846.3 228.0 205.5 6.0
Permethrin PYR 5 258 1.9 372.0 31.0 227.8 333.6 352.8 209.6 58.2 10.0
Phosmet OP 5 258 1.9 209.0 2.9 28.3 157.8 183.4 69.0 37.3 2.0
DDEp,p’ OC 5 208 2.4 31.0 5.5 11.3 30.9 31.0 17.2 5.7 3.0
Flutolanil SFUNG 4 208 1.9 105.0 7.2 54.2 102.1 103.5 55.2 26.1 4.0
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Table1. Cont.
WaxPesticide* Class# Detects Samples % Detections(ppb)
Analyzed High Low Median 90%tile 95%tile Mean1 SEM1 LOD{
Thiacloprid SNEO 4 208 1.9 7.8 1.9 5.9 7.5 7.7 5.4 1.3 1.0
Diazinon OP 4 208 1.9 4.3 1.4 1.6 3.5 3.9 2.2 0.7 1.0
Thiabendazole SFUNG 3 208 1.4 76.0 7.4 19.0 64.6 70.3 34.1 21.2 1.0
Fipronil INS 3 208 1.4 35.9 1.1 1.3 29.0 32.4 12.8 11.6 1.0
Dieldrin CYC 3 258 1.2 35.4 6.9 12.1 30.7 33.1 18.1 8.8 4.0
Pyrimethanil FUNG 3 208 1.4 27.8 3.4 11.7 24.6 26.2 14.3 7.2 2.0
Tebuthiuron SHERB 3 208 1.4 22.4 4.9 5.8 19.1 20.7 11.0 5.7 1.0
Chlorfenapyr PSMITI 3 176 1.7 11.9 1.3 3.6 10.2 11.1 5.6 3.2 1.0
Parathionmethyl OP 3 208 1.4 6.1 3.8 4.0 5.7 5.9 4.6 0.7 1.0
Quintozene = PCNB FUNG 3 208 1.4 2.5 1.0 1.3 2.3 2.4 1.6 0.5 1.0
Ethofumesate SHERB 2 208 1.0 560.0 224.0 392.0 526.4 543.2 392.0 168.0 5.0
Propiconazole SFUNG 2 208 1.0 227.0 166.0 196.5 220.9 224.0 196.5 30.5 3.0
Piperonylbutoxide SYN 2 208 1.0 208.0 31.1 119.6 190.3 199.2 119.6 88.5 6.0
Dimethomorph SFUNG 2 176 1.1 133.0 58.0 95.5 125.5 129.3 95.5 37.5 15.0
Ethion OP 2 208 1.0 131.0 83.6 107.3 126.3 128.6 107.3 23.7 2.0
Captan FUNG 2 258 0.8 69.1 25.0 47.1 64.7 66.9 47.1 22.1 10.0
Fluoxastrobin SFUNG 2 208 1.0 44.5 23.1 33.8 42.4 43.4 33.8 10.7 4.0
Bendiocarb SCARB 2 257 0.8 22.0 5.5 13.8 20.4 21.2 13.8 8.3 2.0
Carbofuran,3-hydroxy SCARB 2 208 1.0 21.1 12.4 16.8 20.2 20.7 16.8 4.4 3.0
Carfentrazoneethyl PSHERB 2 208 1.0 17.0 4.9 11.0 15.8 16.4 11.0 6.1 1.0
Imidacloprid SNEO 2 208 1.0 13.6 2.4 8.0 12.5 13.0 8.0 5.6 2.0
Tetradifon MITI 2 208 1.0 11.1 4.7 7.9 10.5 10.8 7.9 3.2 1.0
Metribuzin SHERB 2 208 1.0 8.0 1.0 4.5 7.3 7.7 4.5 3.5 1.0
Pyriproxyfen IGR 2 208 1.0 7.6 2.2 4.9 7.1 7.3 4.9 2.7 1.0
Prallethrin PYR 2 208 1.0 6.8 4.3 5.6 6.6 6.7 5.6 1.3 4.0
Fluridone SHERB 2 208 1.0 6.6 5.7 6.2 6.5 6.6 6.2 0.4 5.0
Fenamidone FUNG 1 208 0.5 138.0 138.0 138.0 138.0 138.0 138.0 --- 10.0
Heptachlor CYC 1 208 0.5 31.0 31.0 31.0 31.0 31.0 31.0 --- 4.0
Spirodiclofen MITI 1 208 0.5 28.5 28.5 28.5 28.5 28.5 28.5 --- 1.0
Heptachlorepoxide CYC 1 208 0.5 13.3 13.3 13.3 13.3 13.3 13.3 --- 1.0
Fenhexamid FUNG 1 176 0.6 9.3 9.3 9.3 9.3 9.3 9.3 --- 5.0
Carbofuran SCARB 1 208 0.5 5.8 5.8 5.8 5.8 5.8 5.8 --- 5.0
Pyridaben MITI 1 208 0.5 5.4 5.4 5.4 5.4 5.4 5.4 --- 1.0
Carbaryl PSCARB 1 208 0.5 4.5 4.5 4.5 4.5 4.5 4.5 --- 5.0
Tefluthrin PYR 1 208 0.5 3.3 3.3 3.3 3.3 3.3 3.3 --- 1.0
Triadimefon SFUNG 1 208 0.5 2.4 2.4 2.4 2.4 2.4 2.4 --- 2.0
Metalaxyl SFUNG 1 208 0.5 1.4 1.4 1.4 1.4 1.4 1.4 --- 1.0
Hexachlorobenzene FUNG 1 258 0.4 1.0 1.0 1.0 1.0 1.0 1.0 --- 0.1
*Carbendazimisalsoadegradateofbenomyl;Thiabendazoleisadegradateofthiophanatemethyl.
#Class:CAR = carbamate,CYC = cyclodiene,FORM = formamidine,FUNG = fungicide,HERB = herbicide,IGR = insectgrowthregulator,INS = misc.insecticide,
MITI = miticide,NEO = neonicotinoid,OC = organochlorine,OP = organophosphate,PS = partialsystemic,PYR = pyrethroid,S = systemic.
1MeanandSEMfordetections.LOD.
{LOD = limitofdetection(ppb).
doi:10.1371/journal.pone.0009754.t001
Focused analysis of detections from only the pollen and wax pesticide, 57% in combination with a pyrethroid. The most
further indicates the high potential of bee exposure to hive frequent binary combination was fluvalinate and coumaphos
pesticide residues. Two or more pesticides were found in 98.4%, (83.1% of samples), followed by fluvalinate with chlorothalonil
three or more in 91%, and four or more in 80% of the 609 (50.0%), coumaphos with chlorothalonil (47.8%), and fluvalinate
samplesanalyzed.Almost60%ofthesepollenandwaxsamples,in with chlorpyrifos (46.7%). All 375 pollen or wax samples with a
contrastto10.7%ofbeesamples,containedatleastonesystemic fungicide residue (61.7%) had at least one other insecticide or
PLoSONE | www.plosone.org 5 March2010 | Volume 5 | Issue 3 | e9754
PesticideExposurestoBees
Table2. Summaryof pesticide detectionsinpollen samplesfrom North American honey beecolonies.
PollenPesticide* Class# Detects Samples % Detections(ppb)
High Low Median 90%tile 95%tile Mean1 SEM1 LOD{
Fluvalinate PYR 309 350 88.3 2670.0 1.6 40.2 186.8 323.0 95.1 12.6 1.0
Coumaphos OP 263 350 75.1 5828.0 1.0 13.1 518.4 892.0 180.4 33.0 1.0
Chlorpyrifos OP 153 350 43.7 830.0 0.1 4.4 140.4 226.5 53.3 10.6 0.1
Chlorothalonil FUNG 148 280 52.9 98900.0 1.1 35.0 9939.0 18765.0 3014.8 880.9 1.0
Pendimethalin HERB 113 247 45.7 1730.0 1.1 13.4 72.9 129.8 44.6 15.7 1.0
EndosulfanI CYC 98 350 28.0 76.7 0.4 4.2 33.9 47.2 10.9 1.5 0.1
Endosulfansulfate CYC 92 350 26.3 35.0 0.2 2.2 9.2 11.3 4.3 0.6 0.1
DMPF(amitraz) FORM 77 247 31.2 1117.0 6.1 75.0 360.2 615.0 147.9 23.5 4.0
Atrazine SHERB 71 350 20.3 49.0 4.2 8.9 27.0 35.2 13.6 1.1 1.0
EndosulfanII CYC 70 350 20.0 67.7 0.1 3.8 24.7 39.6 9.1 1.6 0.1
Fenpropathrin PYR 63 350 18.0 170.0 0.4 7.0 24.6 60.8 15.1 3.3 0.4
Azoxystrobin SFUNG 53 350 15.1 107.0 1.0 10.2 58.9 68.1 21.0 3.3 1.0
Metolachlor PSHERB 52 350 14.9 103.0 2.6 8.1 19.4 44.6 13.4 2.5 2.0
Captan FUNG 45 350 12.9 10000.0 16.0 103.0 571.8 663.2 433.5 219.9 10.0
Esfenvalerate PYR 41 350 11.7 59.6 1.0 3.3 10.0 47.5 7.8 2.2 0.5
Carbaryl PSCARB 38 350 10.9 1010.0 13.6 36.7 269.5 602.9 117.1 36.5 5.0
Cyhalothrin PYR 38 350 10.9 28.0 0.1 1.7 4.3 18.2 3.4 0.9 0.1
THPI(captan) PSFUNG 35 247 14.2 363.0 60.1 227.0 312.0 342.0 205.8 15.1 30.0
Methoxyfenozide IGR 29 350 8.3 128.0 0.4 22.3 96.4 111.2 35.0 7.1 0.4
Dicofol OC 28 350 8.0 143.0 0.4 8.1 60.3 85.7 23.2 6.4 0.4
Trifloxystrobin PSFUNG 27 350 7.7 264.0 0.6 10.3 96.2 168.4 34.1 11.9 0.5
Tebufenozide IGR 27 350 7.7 58.4 2.0 12.5 28.9 30.0 14.8 2.4 2.0
Diazinon OP 27 350 7.7 29.0 1.0 4.6 25.6 27.7 9.2 1.8 1.0
Cypermethrin PYR 25 350 7.1 49.0 1.6 4.6 27.8 44.7 10.8 2.6 1.0
Cyfluthrin PYR 24 350 6.9 33.6 1.1 5.1 9.9 9.9 6.7 1.3 1.0
Azinphosmethyl OP 23 350 6.6 643.0 3.9 22.0 104.7 615.3 86.2 37.1 3.0
Aldicarbsulfoxide SCARB 21 350 6.0 1245.0 22.0 327.0 1039.0 1146.0 493.7 85.7 20.0
Phosmet OP 20 350 5.7 418.0 3.7 38.0 284.2 351.7 110.0 28.3 2.0
Thiacloprid SNEO 19 350 5.4 115.0 1.7 14.0 42.9 108.7 23.8 7.2 1.0
Pyrimethanil FUNG 19 350 5.4 83.0 2.0 8.3 67.6 82.2 18.5 6.0 2.0
Norflurazon SHERB 18 350 5.1 108.0 2.8 23.5 54.3 66.9 29.7 6.0 1.0
1-Naphthol(carbaryl) SCARB 18 350 5.1 85.5 3.6 9.7 45.6 58.7 20.9 5.2 2.0
Metribuzin SHERB 18 350 5.1 44.0 1.0 3.3 10.1 15.6 6.3 2.3 1.0
Bifenthrin PYR 18 350 5.1 12.6 0.7 3.0 7.1 7.6 3.9 0.7 0.4
Carbendazim SFUNG 16 350 4.6 149.0 1.5 4.5 46.0 89.0 18.8 9.7 1.0
Cyprodinil SFUNG 15 350 4.3 344.0 5.3 18.7 246.8 286.6 90.2 29.1 5.0
Myclobutanil SFUNG 14 350 4.0 981.0 4.4 72.8 565.6 798.4 192.3 78.3 2.0
Propiconazole SFUNG 14 350 4.0 361.0 3.1 68.0 203.2 259.6 110.3 27.3 3.0
Fenbuconazole SFUNG 14 247 5.7 264.0 11.0 55.4 174.9 217.8 80.6 19.9 6.0
Coumaphosoxon OP 14 280 5.0 89.0 5.4 13.5 38.2 52.2 21.2 5.9 5.0
Methidathion OP 14 350 4.0 32.7 7.8 21.0 31.7 32.3 21.6 2.1 1.0
Malathion OP 13 350 3.7 61.0 0.9 5.9 16.2 35.2 10.4 4.4 1.0
Aldicarbsulfone SCARB 12 350 3.4 97.2 17.0 43.8 87.7 93.2 46.8 7.8 10.0
Simazine SHERB 12 350 3.4 54.0 5.2 22.0 36.9 44.7 22.4 4.3 5.0
Pronamide SHERB 11 350 3.1 378.0 17.7 71.0 355.0 366.5 122.9 38.7 1.0
Indoxacarb INS 11 350 3.1 330.0 10.0 102.0 175.0 252.5 118.2 24.7 10.0
Acetamiprid SNEO 11 350 3.1 134.0 14.0 57.0 101.0 117.5 59.3 11.8 5.0
Deltamethrin PYR 11 350 3.1 91.0 28.0 66.0 88.5 88.4 66.6 6.2 20.0
Imidacloprid SNEO 10 350 2.9 206.0 6.2 20.5 63.0 41.3 39.0 19.0 2.0
PLoSONE | www.plosone.org 6 March2010 | Volume 5 | Issue 3 | e9754
PesticideExposurestoBees
Table2. Cont.
PollenPesticide* Class# Detects Samples % Detections(ppb)
High Low Median 90%tile 95%tile Mean1 SEM1 LOD{
Fenhexamid FUNG 9 247 3.6 129.0 5.8 28.0 53.8 96.1 34.4 12.3 5.0
Permethrin PYR 9 350 2.6 92.0 9.6 28.7 89.6 73.8 40.1 10.7 10.0
Trifluralin HERB 9 247 3.6 14.4 1.0 1.9 10.9 12.6 3.9 1.6 1.0
Tebuthiuron SHERB 8 350 2.3 48.0 1.6 16.2 34.7 40.4 17.9 5.8 1.0
Thiabendazole SFUNG 8 350 2.3 5.6 1.4 2.4 4.8 5.2 3.0 0.5 1.0
Dimethomorph SFUNG 7 247 2.8 166.0 17.2 25.2 95.1 142.4 46.9 20.2 15.0
Oxyfluorfen HERB 7 350 2.0 4.5 0.5 1.8 3.1 3.8 2.0 0.5 0.5
Difenoconazole SFUNG 6 350 1.7 214.1 48.3 122.4 184.8 199.4 129.8 22.2 10.0
Famoxadone FUNG 6 350 1.7 141.0 73.5 95.7 125.5 133.3 98.3 10.9 20.0
Diphenylamine FUNG 6 103 5.8 32.0 3.6 10.5 24.5 28.3 13.2 4.3 2.0
Hexachlorobenzene FUNG 6 350 1.7 0.4 0.1 0.2 0.4 0.4 0.2 0.1 0.1
Pyridaben MITI 5 350 1.4 26.6 10.9 19.0 25.6 26.1 18.8 3.0 1.0
Diflubenzuron IGR 4 350 1.1 128.0 15.0 78.5 122.0 125.0 75.0 26.1 10.0
Oxamyl SCARB 4 350 1.1 43.0 20.0 32.5 40.3 41.7 32.0 4.7 5.0
Allethrin PYR 4 350 1.1 11.0 6.6 7.9 10.2 10.6 8.3 0.9 1.0
Vinclozolin FUNG 4 350 1.1 4.1 1.0 1.0 3.2 3.6 1.8 0.8 1.0
Boscalid SFUNG 3 350 0.9 962.0 1.4 12.0 772.0 11.5 325.1 318.4 1.0
Potasan(coumaphos) OP 3 247 1.2 160.0 61.5 138.0 155.6 157.8 119.8 29.9 10.0
Pyrethrins PYR 3 350 0.9 61.5 27.0 35.0 56.2 61.5 41.2 10.4 20.0
Tebuconazole SFUNG 3 350 0.9 34.0 6.4 18.0 30.8 32.4 19.5 8.0 3.0
Prallethrin PYR 3 350 0.9 7.6 4.7 7.3 7.5 7.6 6.5 0.9 4.0
Carfentrazoneethyl PSHERB 3 350 0.9 2.5 1.5 2.4 2.5 2.5 2.1 0.3 1.0
Propanil HERB 2 350 0.6 358.0 265.0 311.5 348.7 353.4 311.5 46.5 10.0
Pyraclostrobin FUNG 2 350 0.6 265.0 26.6 145.8 241.2 26.6 145.8 119.2 1.0
DDTp,p’ OC 2 350 0.6 35.6 6.0 20.8 32.6 34.1 20.8 14.8 2.0
Fluridone SHERB 2 350 0.6 24.0 5.8 14.9 22.2 22.2 14.9 9.1 5.0
DDDp,p’ OC 2 350 0.6 13.4 11.8 12.6 13.2 13.3 12.6 0.8 4.0
4,4-dibromobenzophenone MITI 2 247 0.8 10.8 2.2 6.5 9.9 10.4 6.5 4.3 2.0
Carbofuran,3-hydroxy SCARB 2 350 0.6 4.6 3.6 4.1 4.5 4.6 4.1 0.5 3.0
DDEp,p’ OC 2 350 0.6 4.3 3.3 3.8 4.2 4.3 3.8 0.5 3.0
Chlorfenapyr PSMITI 2 247 0.8 1.4 1.2 1.3 1.4 1.4 1.3 0.1 1.0
Diphenamid SFUNG 2 350 0.6 1.0 1.0 1.0 1.0 1.0 1.0 0.0 1.0
Imidaclopridolefin SNEO 1 350 0.3 554.0 554.0 554.0 554.0 554.0 554.0 --- 25.0
Sethoxydim SHERB 1 350 0.3 173.0 173.0 173.0 173.0 173.0 173.0 --- 1.0
Acephate SOP 1 350 0.3 163.0 163.0 163.0 163.0 163.0 163.0 --- 35.0
Imidacloprid,5-hydroxy SNEO 1 350 0.3 152.0 152.0 152.0 152.0 152.0 152.0 --- 25.0
Amicarbazone HERB 1 350 0.3 98.0 98.0 98.0 98.0 98.0 98.0 --- 30.0
Phenothrin PYR 1 350 0.3 83.9 83.9 83.9 83.9 83.9 83.9 --- 10.0
Fenamidone FUNG 1 350 0.3 73.9 73.9 73.9 73.9 73.9 73.9 --- 10.0
Thiamethoxam SNEO 1 350 0.3 53.3 53.3 53.3 53.3 53.3 53.3 --- 5.0
Phosalone OP 1 247 0.4 31.3 31.3 31.3 31.3 31.3 31.3 --- 10.0
Fipronil INS 1 350 0.3 28.5 28.5 28.5 28.5 28.5 28.5 --- 1.0
Chlorfenvinphos OP 1 247 0.4 10.7 10.7 10.7 10.7 10.7 10.7 --- 6.0
Iprodione FUNG 1 350 0.3 10.3 10.3 10.3 10.3 10.3 10.3 --- 10.0
Spiromesifen SINS 1 350 0.3 10.0 10.0 10.0 10.0 10.0 10.0 --- 10.0
Tetramethrin PYR 1 350 0.3 6.1 6.1 6.1 6.1 6.1 6.1 --- 6.0
Tribufos = DEF SYN 1 350 0.3 3.5 3.5 3.5 3.5 3.5 3.5 --- 2.0
PLoSONE | www.plosone.org 7 March2010 | Volume 5 | Issue 3 | e9754
PesticideExposurestoBees
Table2. Cont.
PollenPesticide* Class# Detects Samples % Detections(ppb)
High Low Median 90%tile 95%tile Mean1 SEM1 LOD{
Spirodiclofen MITI 1 350 0.3 1.9 1.9 1.9 1.9 1.9 1.9 --- 1.0
Heptachlorepoxide CYC 1 350 0.3 1.7 1.7 1.7 1.7 1.7 1.7 --- 1.0
*Carbendazimisalsoadegradateofbenomyl;Thiabendazoleisadegradateofthiophanatemethyl.
#Class:CAR = carbamate,CYC = cyclodiene,FORM = formamidine,FUNG = fungicide,HERB = herbicide,IGR = insectgrowthregulator,INS = misc.insecticide,
MITI = miticide,NEO = neonicotinoid,OC = organochlorine,OP = organophosphate,PS = partialsystemic,PYR = pyrethroid,S = systemic.
1MeanandSEMfordetections.LOD.{LOD = limitofdetection(ppb).
doi:10.1371/journal.pone.0009754.t002
miticide present, and except for 6 or 8 of these samples, Pesticide residues ranged over six orders of magnitude (1
respectively, contained a pyrethroid or organophosphate. The million-fold), and wide-differences in mean, and 90%- and 95%-
most prevalent triple detections were fluvalinate and coumaphos tile values (levels at which only 10% or 5% of detections,
combined with chlorothalonil (47.2%), chlorpyrifos (41.0%), respectively,arehigher)permatrixwerefound(Tables1,2,3,4).
degradates of amitraz (41.0%), or with one of 43 systemic By comparing these residue levels across the matrices, an
pesticides (47.9%). At least one each of an insecticide/acaricide, interesting trend emerges with regard to in-hive versus external-
fungicide or herbicide were found in 34.8% of samples, with the ly-derivedpesticides.Fluvalinate,coumaphosandamitrazwere87-
fluvalinate, chlorothalonil and pendimethalin combination most , 25- and 33-fold more concentrated in wax, respectively, than
frequent(20.6%).Thehighestfrequencyofquaternarydetections pollen (Table 4), while higher or more equivalent amounts of
were fluvalinate, coumaphos and amitraz combined with chlor- aldicarb, chlorothalonil, chlorpyrifos, endosulfan, pendimethalin,
othalonil (30.7%) or chlorpyrifos (20.3%), or fluvalinate, couma- fenpropathrin, azoxystrobin and other environmental pesticides
phos and chlorothalonil combined with a systemic (31.4%) or werefoundinthepollencomparedtowax.Thisisconsistentwith
chlorpyrifos (26.2%). chronic use and long-term accumulation of these lipophilic
miticides in the wax, which becomes a source of subsequent
Trendsinresiduelevelsacrossthethreeprimarymatrices contamination of stored pollen. For agricultural pesticides, the
The most frequently found residues were from fluvalinate and greatestindicationofwaxbioaccumulationfromapollensourceis
coumaphos, followed in order by chlorpyrifos, chlorothalonil, with the highly lipophilic insect growth regulator, methoxyfeno-
amitraz, pendimethalin, endosulfan, fenpropathrin, esfenvalerate zide, which was 5.3 times more prevalent in wax (Table 4). In
and atrazine. These top ten comprise three in-hive miticides and general, this trend also occurred withthe pyrethroids.
fiveinsecticidal,onefungicidalandoneherbicidalcropprotection The highly-lipophilic fluvalinate and amitraz degradates
agents(Table4).Inpollen,unprecedentedlevels(upto99ppm) (DMPF and DMA) bioaccumulate in bees to a much greater
of chlorothalonil were found, along with ppm levels of aldicarb, extent than does coumaphos, as indicated by the respective 3.6-
captan, carbaryl, myclobutanil, pendimethalin and the Varroa and 3.3-times greater bee to pollen ratios of mean residue values
miticides(Tables2,4).Nearppmlevelsofimidacloprid,boscalid relative to a 4.5-fold lower ratio for coumaphos (Table 4). The
and chlorpyrifos were also noted in pollen, with lesser but lipophilicfungicidechlorothalonilis100-foldlowerinbeesthanin
substantial amounts of potentially synergistic fungicides such as pollen or wax, perhaps due to rapid bee transformation to
fenbuconazole, cyprodinil and propiconazole. Almost all wax undetected or excreted metabolites. Similar metabolism may
samples(98%)werecontaminatedwithfluvalinateandcoumaphos explain the lower levels of coumaphos in bees compared to the
upto204and94ppm,respectively,alongwithloweramountsand other miticides. Parent fungicides and some metabolites (e.g
frequency of amitraz degradates and chlorothalonil. Near ppm THPI), regardless of lipophilicity or systemic movement, were
levels of chlorpyrifos, aldicarb, deltamethrin, iprodione and generallylackinginbees,incontrasttobeing151-timeshigherin
methoxyfenozide were alsofound incombwax (Tables 1,4). pollen (Table4).
Lower residues of pesticides prevailed in bees except for
occasional samples associated with high mortality (see below) or Pesticide residue levels and acute bee toxicity
withnotablemiticide(upto14ppm),andnearppmcarbaryland Comparison of ppb residue levels across matrices with known
chlorothalonil detections (Tables 3, 4). Although a few residues LD valuesforhoneybeesinppbrelativetobodyweightprovided
50
foratrazine,carbendazim,cyprodinil,pronamide,dimethomorph, only a few detections at or well above the lethal dose (Table 4).
andthedegradatesTHPI(captan)and1-naphthol(carbaryl)were Two samples of dead bees were linked by analysis to prior
detected, systemic pesticides were generally absent from bee environmental applications of permethrin (19.6 ppm residue, LD
50
samples(Table3).Noneonicotinoidresidueswerefoundinbees, of1.1ppm)andfipronil(3.1ppm,LD 0.05ppm).However,other
50
while 23 thiacloprid, 14 imidacloprid, 11 acetamiprid and 1 beesamplesrepresentedbeesremaining,anditshouldbenotedthat
thiamethoxam detections were obtained from pollen and wax foragers that never returned and were presumed dead were not
(Tables 1, 2, 3, 4). Overall, pyrethroids and organophosphates sampled. For bees from CCD–associated colonies, only sublethal
dominated total wax and bee residues followed by fungicides, although high amounts of fluvalinate (up to 6 ppm), amitraz,
systemics, carbamates and herbicides, whereas fungicides pre- coumaphosandchlorothalonilweredetected.Thatthebeecontent
vailed in pollen followed by organophosphates, systemics, for the latter lipophilic fungicide was much less (221 times on
pyrethroids, carbamates and herbicides (Table 4). The 98 average) than beebread food (Table 4) from the same colonies
pesticides and metabolites detected in mixtures up to 214ppm indicatesthatmetabolismoftheparentpesticideisoccurringinthe
in bee pollen alone represents a remarkably high level for toxic bee.Detectedpollenlevelsofpesticidesarepredictedtobesublethal
contaminants inthebrood andadultfoodof thispollinator. (belowone-tenththeLD )exceptforoccasionalhighresiduesofthe
50
PLoSONE | www.plosone.org 8 March2010 | Volume 5 | Issue 3 | e9754
PesticideExposurestoBees
Table3. Summaryof pesticide detectionsinbeesfrom North American honey beecolonies.
BeePesticide* Class# Detects Samples % Detections(ppb)
Analyzed High Low Median 90%tile 95%tile Mean1 SEM1 LOD{
Fluvalinate PYR 117 140 83.6 5860.0 1.1 53.0 610.8 1710.0 357.7 94.5 1.0
Coumaphos OP 84 140 60.0 762.0 1.0 8.0 118.7 156.2 50.4 13.5 1.0
Chlorpyrifos OP 12 140 8.6 10.7 1.0 2.2 8.5 9.7 3.4 0.9 0.1
Chlorothalonil FUNG 10 140 7.1 878.0 1.5 7.2 121.1 499.5 100.2 86.5 1.0
Cypermethrin PYR 9 140 6.4 25.8 2.0 3.5 22.0 23.9 10.1 3.2 1.0
Permethrin PYR 8 140 5.7 19600.0 12.0 35.8 5919.2 12759.6 2478.1 2446.0 10.0
DMPF(amitraz) FORM 8 125 6.4 9040.0 6.0 117.5 3015.8 6027.9 1249.1 1114.1 4.0
Esfenvalerate PYR 8 140 5.7 9.3 1.0 3.5 8.5 8.9 4.3 1.2 0.5
Methidathion OP 7 140 5.0 32.0 6.5 12.0 28.4 30.2 16.2 3.6 1.0
Deltamethrin PYR 6 140 4.3 39.0 23.0 26.5 38.5 38.8 29.3 3.0 20.0
Pendimethalin HERB 6 140 4.3 27.6 6.5 14.0 26.4 27.0 15.9 3.8 1.0
Cyfluthrin PYR 5 140 3.6 14.0 2.0 10.0 13.2 13.6 8.2 2.4 1.0
Dicofol OC 5 140 3.6 3.8 1.0 1.4 3.6 3.7 2.1 0.6 0.4
Fenpropathrin PYR 4 140 2.9 37.0 2.8 14.2 32.8 34.9 17.1 8.0 0.4
Azinphosmethyl OP 4 140 2.9 22.0 4.8 13.1 20.5 21.3 13.3 3.9 3.0
Cyprodinil SFUNG 4 140 2.9 19.0 9.2 11.0 16.6 17.8 12.6 2.2 5.0
THPI(captan) PSFUNG 3 125 2.4 43.4 37.7 39.5 42.6 43.0 40.2 1.7 30.0
Allethrin PYR 3 140 2.1 24.0 6.7 19.0 23.0 23.5 16.6 5.1 1.0
Tetramethrin PYR 3 140 2.1 23.0 18.0 23.0 23.0 23.0 21.3 1.7 6.0
Methoxyfenozide IGR 3 140 2.1 21.0 1.5 3.4 17.5 19.2 8.6 6.2 0.4
EndosulfanI CYC 3 140 2.1 6.1 1.3 1.6 5.2 5.7 3.0 1.6 0.1
Endosulfansulfate CYC 3 140 2.1 3.0 1.6 2.7 2.9 3.0 2.4 0.4 0.1
EndosulfanII CYC 3 140 2.1 2.4 1.4 1.9 2.3 2.4 1.9 0.3 0.1
Parathionmethyl OP 3 140 2.1 2.0 1.5 1.8 2.0 2.0 1.8 0.1 1.0
Cyhalothrin PYR 3 140 2.1 1.8 1.1 1.7 1.8 1.8 1.5 0.2 0.1
DMA(amitraz) FORM 2 125 1.6 4740.0 275.0 2507.5 4293.5 4516.8 2507.5 2232.5 50.0
Fipronil INS 2 140 1.4 3060.0 9.9 1535.0 2755.0 2907.5 1535.0 1525.1 1.0
Bifenthrin PYR 2 140 1.4 12.3 2.9 7.6 11.4 11.8 7.6 4.7 0.4
Dieldrin CYC 2 140 1.4 12.0 10.0 11.0 11.8 11.9 11.0 1.0 4.0
Prallethrin PYR 2 140 1.4 8.6 6.2 7.4 8.4 8.5 7.4 1.2 4.0
Coumaphosoxon OP 2 140 1.4 6.8 2.1 4.5 6.3 6.6 4.5 2.4 5.0
Oxyfluorfen HERB 2 140 1.4 4.8 3.8 4.3 4.7 4.8 4.3 0.5 0.5
Chlorfenapyr PSMITI 2 140 1.4 2.7 1.8 2.3 2.6 2.7 2.3 0.5 1.0
Carbaryl PSCARB 1 140 0.7 588.0 588.0 588.0 588.0 588.0 588.0 --- 5.0
1-Naphthol(carbaryl) SCARB 1 140 0.7 238.0 238.0 238.0 238.0 238.0 238.0 --- 2.0
Dimethomorph SFUNG 1 125 0.8 56.0 56.0 56.0 56.0 56.0 56.0 --- 15.0
Tebuconazole SFUNG 1 140 0.7 34.0 34.0 34.0 34.0 34.0 34.0 --- 3.0
Chlorferone(coumaphos) OP 1 125 0.8 25.0 25.0 25.0 25.0 25.0 25.0 --- 25.0
Tebufenozide IGR 1 140 0.7 23.0 23.0 23.0 23.0 23.0 23.0 --- 2.0
Fenoxaprop-ethyl SHERB 1 140 0.7 15.4 15.4 15.4 15.4 15.4 15.4 --- 6.0
Atrazine SHERB 1 140 0.7 15.0 15.0 15.0 15.0 15.0 15.0 --- 1.0
Carbendazim SFUNG 1 140 0.7 14.3 14.3 14.3 14.3 14.3 14.3 --- 1.0
Pyraclostrobin FUNG 1 140 0.7 8.6 8.6 8.6 8.6 8.6 8.6 --- 1.0
DDEp,p’ OC 1 140 0.7 6.6 6.6 6.6 6.6 6.6 6.6 --- 3.0
Fluridone SHERB 1 140 0.7 6.5 6.5 6.5 6.5 6.5 6.5 --- 5.0
Pronamide SHERB 1 140 0.7 2.2 2.2 2.2 2.2 2.2 2.2 --- 1.0
*Carbendazimisalsoadegradateofbenomyl;Thiabendazoleisadegradateofthiophanatemethyl.
#Class:CAR = carbamate,CYC = cyclodiene,FORM = formamidine,FUNG = fungicide,HERB = herbicide,IGR = insectgrowthregulator,INS = misc.insecticide,
MITI = miticide,NEO = neonicotinoid,OC = organochlorine,OP = organophosphate,PS = partialsystemic,PYR = pyrethroid,S = systemic.
1MeanandSEMfordetections.LOD.
{LOD = limitofdetection(ppb).
doi:10.1371/journal.pone.0009754.t003
PLoSONE | www.plosone.org 9 March2010 | Volume 5 | Issue 3 | e9754
PesticideExposurestoBees
M 2
1b) SE 79.7 8.4 0.1 6.3 104. 0.3 0.1 0.3 0.1 0.1 0.2 0.0 0.1 0.0 0.0 0.1 0.0 0.0 0.0 0.0 0.0 0.0 0.3 0.0 0.2 0.0 0.3 0.0 0.2 0.0 0.1 0.2 0.2 0.0 0.5
p
Bee(p Mean 299.0 30.5 0.3 7.2 107.2 0.7 0.2 0.5 0.2 0.1 0.2 0.0 0.1 0.0 0.0 0.1 0.0 0.1 0.0 0.0 0.0 0.1 0.8 0.0 0.4 0.0 0.6 0.0 0.2 0.0 0.1 0.3 0.4 0.0 1.3
1pb) SEM 11.2 25.4 4.8 473.5 7.3 7.3 1.0 0.7 0.3 0.4 0.8 0.6 0.1 0.1 8.4 0.5 2.7 0.6 0.0 0.5 0.2 0.0 0.2 1.6 1.6 0.1 0.2 0.0 0.3 1.6 0.8 0.1 0.0 1.0 0.6
p
(
Pollen Mean 83.9 137.4 23.3 1593.5 32.5 20.4 6.0 2.7 0.9 2.8 2.9 3.2 0.2 0.1 31.2 0.9 2.8 1.9 0.0 1.5 0.4 0.0 0.9 4.6 3.9 0.4 0.8 0.0 1.1 3.9 0.8 0.5 0.1 2.6 2.1
M 9 8 2 1
1b) SE 956. 511. 4.8 225. 327. 0.7 1.0 1.0 0.3 0.3 3.9 1.1 0.4 0.2 7.9 0.8 3.1 0.2 5.8 0.2 1.7 0.2 0.3 1.3 0.8 0.1 0.7 0.2 0.2 0.1 2.3 0.2 0.7 0.1 2.9
p
p
( n 5 4 7
Wax Mea 7329. 3363. 15.5 525.0 1080. 3.0 5.5 4.2 1.5 1.1 15.3 2.4 1.3 0.5 27.8 2.3 11.1 0.7 18.2 0.4 5.3 0.7 0.9 3.1 1.8 0.3 1.6 0.4 0.4 0.3 4.0 0.5 1.1 0.2 6.5
Bee 1623 135 1 3 6 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
b) en 0
(pp Poll 294 730 127 1038 181 71 33 12 3 17 11 17 0 0 92 0 0 3 0 2 0 0 0 0 0 2 3 0 8 0 0 2 0 5 0
e
es. 95%til Wax 28703 11555 33 1545 4700 11 22 30 11 8 89 7 6 1 217 11 80 5 136 2 22 3 4 11 4 0 1 0 0 0 0 0 0 0 0
ni
o 0
col pb) Bee 5860 762 11 878 1378 28 9 37 9 15 21 0 12 0 0 14 0 4 0 0 0 5 32 0 19 2 26 0 23 2 9 14 24 0 39
e p
e ( n
eyb ction Polle 2670 5828 830 98900 1117 1730 157 170 60 49 128 107 13 14 1342 149 962 143 10 108 62 5 33 264 344 28 49 4 58 378 265 34 11 264 91
n e
o et
h D 0
erican Max Wax 20400 94131 890 53700 46060 84 132 200 56 31 495 278 56 36 693 133 388 21 636 38 222 34 79 183 106 17 131 27 28 23 438 45 139 22 613
m
A 0 0 0 0 0 0 0 0 0 0 0 0 0
North "LD50 (ppb) 15860 46300 1220 111000 750000 665000 78700 500 2240 980000 100000 112000 150 685000 3730 500000 155000 370000 102000 163000 1480 100000 2010 149000 332000 790 1350 100000 234000 158000 870000 220 48800 175000 500
mplesfrom Detections PollenBee 88.383.6 75.460.0 43.78.6 52.97.1 31.26.4 45.74.3 36.63.6 18.02.9 11.75.7 20.30.7 8.32.1 15.10.0 5.11.4 3.60.0 6.00.0 4.60.7 0.90.0 8.03.6 0.30.0 5.10.0 0.90.0 2.01.4 4.05.0 5.70.0 4.32.9 10.92.1 7.16.4 1.10.0 7.70.7 3.10.7 0.60.7 6.93.6 1.12.1 7.70.0 3.14.3
beesa %with Wax 98.1 98.1 63.2 49.2 61.6 27.8 39.1 17.1 16.7 13.9 18.8 15.5 12.8 12.5 10.6 10.1 10.1 10.1 6.7 6.3 6.3 6.2 5.8 5.7 5.3 5.0 5.0 5.0 4.8 4.8 4.8 4.3 3.8 3.1 3.1
and ed Bee 140 140 140 140 125 140 140 140 140 140 140 140 140 125 140 140 140 140 140 140 140 140 140 125 140 140 140 140 140 140 140 140 140 140 140
z
en aly en
poll sAn Poll 350 350 350 280 247 247 350 350 350 350 350 350 350 247 350 350 350 350 350 350 350 350 350 247 350 350 350 350 350 350 350 350 350 350 350
9wax, Sample Wax 259 259 258 258 177 176 258 258 258 208 208 258 258 176 208 208 208 258 208 208 208 258 258 176 208 258 258 258 208 208 208 258 208 258 258
4
7
n # G
encei CLASS PYR OP OP FUNG FORM HERB CYC PYR PYR SHERB IGR SFUNG PYR HERB SCARB SFUNG SFUNG OC FUNG SHERB PYR HERB OP SFUNG SFUNG PYR PYR FUNG IGR SHERB FUNG PYR PYR PSFUN PYR
d
ci
n
i
e
d
ci
Table4.Pesti TotalPesticide* Fluvalinate Coumaphos Chlorpyrifos Chlorothalonil Amitraz Pendimethalin Endosulfan Fenpropathrin Esfenvalerate Atrazine Methoxyfenozide Azoxystrobin Bifenthrin Trifluralin Aldicarb Carbendazim Boscalid Dicofol Iprodione Norflurazon Pyrethrins Oxyfluorfen Methidathion Fenbuconazole Cyprodinil Cyhalothrin Cypermethrin Vinclozolin Tebufenozide Pronamide Pyraclostrobin Cyfluthrin Allethrin Trifloxystrobin Deltamethrin
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