Morpho-physiological, anatomical and biochemical changes as influenced by paclobutrazol in ...

299 Pages·2012·11.05 MB·English

Morpho-physiological, anatomical and biochemical
changes as influenced by paclobutrazol in mungbean
[Vigna radiata (L.) Wilczek] under flooding stress

ABSTRACT
of
THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE
REQUIREMENTS FOR THE DEGREE OF
Doctor of Philosophy
in
Plant Physiology

By
Dinesh Kumar Yadav

Co-Supervisor Supervisor
Prof. J.P. Srivastava Prof. A. Hemantaranjan

DEPARTMENT OF PLANT PHYSIOLOGY
INSTITUTE OF AGRICULTURAL SCIENCES
BANARAS HINDU UNIVERSITY
VARANASI - 221005

I.D.No. PP-11062 2016 Enrolment No. 302643
Copyright © Institute of Agricultural Sciences, Banaras Hindu University,
Varanasi, India, 2016. All rights reserved.
UNDERTAKING BY THE CANDIDATE
I, Dinesh Kumar Yadav, I.D. No. PP-11062 and Enrollment number 302643
declares that I have pursued my research work under the supervision of Prof. A.
Hemantaranjan and co-supervision of Prof. J. P. Srivastava from 21-10-2011 to
15-02-2016. I hereby supplicate my thesis entitled “Morpho-physiological,
anatomical and biochemical changes as influenced by paclobutrazol in
mungbean [Vigna radiata (L.) Wilczek] under flooding stress”

Date:
Place: B.H.U., Varanasi (Dinesh Kumar Yadav)
CANDIDATES’S DECLARATION
I, Dinesh Kumar Yadav, PP-11062, Enrolment Number 302643, certify that
the work embodied in this Ph.D. thesis is my own bonafide work carried out by me
under the supervision of Prof. A. Hemantaranjan and co-supervision of Prof. J. P.
Srivastava for a period of four years three months from 21-10-2011 to 15-02-2016 at
Institute of Agricultural Sciences, Banaras Hindu University. The matter embodied in
this Ph.D. thesis has not been submitted for the award of any other degree/diploma.
I declare that I have faithfully acknowledged, given credit to and referred to
the research workers wherever, their works have been cited in the text and the body of
the thesis. I further certify that I have not willfully lifted up some other’s work, para,
text, data, results, etc. reported in the journals, books, magazines, reports,
dissertations, thesis, etc., or available at web sites and included them in this Ph.D.
thesis and cited as my own work.
Date: …………….. (Signature of the Candidate)
Place: B.H.U., Varanasi (Dinesh Kumar Yadav)

CERTIFICATE FROM SUPERVISOR/CO-SUPERVISOR
This is to certify that the above statement made by the candidate is correct to
the best of our knowledge.
………………………
(Supervisor’s signature)
Dr. A. Hemantaranjan
Professor
Department of Plant Physiology

………………………
(Co-supervisor’s signature)
Dr. J. P. Srivastava
Professor
Department of Plant Physiology

(Signature of HOD/ Coordinator of the school with seal)
COURSE/COMPREHENSIVE EXAMINATION COMPLETION
CERTIFICATE

This is to certify that Mr. Dinesh Kumar Yadav, PP-11062 with Enrolment
Number 302643, a bonafide Research Scholar of this Department, has successfully
completed the course work / Comprehensive Examination requirements which are the
part of his Ph.D. Programme.

Date: (Signature of the Head of the Department)
Place: B.H.U., Varanasi
PRE-SUBMISSION SEMINAR COMPLETION CERTIFICATE
This is to certify that Mr. Dinesh Kumar Yadav, PP-11062 with Enrolment
number 302643, a bonafide Research Scholar of this Department, has successfully
completed the Pre-Submission Seminar on 11-01-2016 on the topic “Morpho-
physiological, anatomical and biochemical changes as influenced by
paclobutrazol in mungbean [Vigna radiata (L.) Wilczek] under flooding stress”
which is a part of his Ph.D. Programme.

Date: (Signature of the Head of the Department)
Place: B.H.U., Varanasi
COPYRIGHT TRANSFER CERTIFICATE
Title of the thesis : “Morpho-physiological, anatomical and biochemical
changes as influenced by paclobutrazol in mungbean
[Vigna radiata (L.) Wilczek] under flooding stress”
Candidate’s Name : Dinesh Kumar Yadav

COPYRIGHT TRANSFER
The undersigned hereby assigns to the Banaras Hindu University all rights
under copyright that may exist in and for the above thesis submitted for the award of
the Ph.D. degree.

Signature of the candidate

Notice: However, the author may reproduce or authorize others to reproduce
material extracted verbatim from the thesis or derivative of the thesis
for author’s personal use provided that the source and the university’s
copyright notice are indicated.
Morpho-physiological, anatomical and biochemical changes as influenced
by paclobutrazol in mungbean [Vigna radiata (L.) Wilczek] under
flooding stress

Thesis submitted in partial fulfilment of the requirements for the
award of the degree of
Doctor of Philosophy
in
Plant Physiology
2016
by
Dinesh Kumar Yadav
APPROVED BY RESEARCH PROGRAMME COMMITTEE
Chairman Dr. A. Hemantaranjan
Professor
Department of Plant Physiology

Co-Chairman Dr. J. P. Srivastava
Professor
Department of Plant Physiology

DRC Nominee Dr. P. Dwivedi
Professor
Department of Plant Physiology

Internal Subject Expert Dr. Pravin Prakash
Assistant Professor
Department of Plant Physiology

External Subject Expert Dr. Yashwant Singh
Professor
Department of Agronomy

External Subject Expert Dr. Anand K. Singh
Professor
Department of Horticulture

External Examiner
ACKNOWLEDGEMENTS

At the outset, very humbly I bow my head with reverence to the “Almighty”,
whose blessings has enabled me to accomplish my Ph.D. research and Course Programme
with all devotion and enthusiasm in the great seat of learning, the Banaras Hindu
University, created by the “Bharat Ratna” Mahamana Pandit Madan Mohan Malaviya
ji. I express my deepest sense of gratitude to our great founder who provided an absolute
peaceful and blissful environment for being thoughtful, studious and critical in
meticulously handling meaningful research for the holistic development of science, culture,
humanity and above all the agriculture.
First and foremost with due deference, I am indebted and delightfully express my
deep sense of gratitude to my Ph.D. Guide, Dr. A. Hemantaranjan, Professor and
Formerly Head, Department of Plant Physiology for his distinctive and thoughtful
recommendation in selecting the most needed consequential research problem for my
doctoral research, which truly inspired me to work hard in the last four years in this
direction. Professor A. Hemantaranjan as my Advisor-cum-Chairman endeavoured and
thoroughly monitored the work for the quality output and accordingly, it became
promising for me to accomplish the same to my level best. He has been consistently
motivating me with all possible assistance to remain focused on achieving my goal. His
keen observations and candid comments helped me to establish the overall route of the
research so as to move forward for investigations in depth. It was, indeed, a unique
opportunity with gratification to work under a man of experience, zeal, enthusiasm and
devotion for the pious cause of research and education. Ever since, Hemantaranjan Sir has
supported me not only by providing a research assistantship over almost four years, but
also strengthened me academically and emotionally through the rough road to finish this
thesis. Of course, during the most difficult times of writing this thesis, he bestowed upon
me the outstanding moral support, love, affections and independence of thought that was
indispensably needed for forging ahead steadily. I honestly confess that his logical
suggestions in writing research papers for standard journals, in general, and constructive
criticism in achieving academic heights, in particular, certainly made me more critical for
accepting greater task of values in life. I convey my gratefulness to him for stimulating me
to work with vision along with a talented team of researchers in an absolute academic
milieu.
I wish to record my heartiest and ardent thanks to my Co-supervisor Dr. J.P.
Srivastava, Professor and Formerly Head, Department of Plant Physiology for his
scientific support during my research work. I express my deepest sense of gratitude to the
esteemed members of my Research Programme Committee (RPC), Dr. Padmanabh Dwivedi,
Professor, Dr. Pravin Prakash, Assistant Professor, Department of Plant Physiology,
Dr. Yashwant Singh, Professor, Department of Agronomy and Dr. Anand Kumar Singh,
Professor, Department of Horticulture for their valuable and constructive suggestions
during the investigations and preparations of this manuscript.
I am further grateful to the Hon'ble Director, Institute of Agricultural Sciences,
Banaras Hindu University, Prof. R.P. Singh, the esteemed Dean, Faculty of Agriculture,
Prof. A. Vaishampayan and the admired Head, Department of Plant Physiology,
Prof. (Mrs.) Bandana Bose for their motivation, all time constructive suggestions and
mobilizing to supplicating the present manuscript in time. Subsequently, I express my
thankfulness to our admired Faculty members Dr. (Mrs.) Vijai P., Assistant Professor and
Dr. (Mrs.) Savita Jangde, Assistant Professor for their precious academic help from time to
time and consistent encouragement throughout the preparation of this manuscript.
I would like to thank Prof. M.N. Singh, Department of Genetics and Plant
Breeding who was kind enough to provide seeds of mungbean genotypes and
Prof. Bandana Bose, Head, Department of Plant Physiology for providing lab facility for
successfully completion of protein profiling (SDS-PAGE) of my Ph.D. research work. My
heartfelt thanks is also due to Mahesh Kumar Shukla for their precious assistance during
protein profiling work.
I have my sincere special thanks to dear seniors Dr. Deepmala Katiyar,
Dr. Pradeep Kumar Patel, Dr. Radha Singh, Dr. Ruchi Bansal, Dr. Vinay Pratap Singh,
Dr. Surender Bisnoi, Dr. Bharti Singh and Dr. Pratap Singh for their enormous support,
encouragement and enthusiasm at every stage of my life and study. My thanks are due to
Sarika Ma'm for giving impetus with emotional support during the course of my study.
Besides these, a very special thanks to our senior-cum-brother Arvind Singh Sir for his hold
up, love, affection and emotional support at every stage of my life.
All the words in the lexicon will be futile and meaningless, if I fail to express the
obligation from the core of my heart to my dear friends Anirudha, Janmajay, Navin,
Akanksha, Bhawna, Ankita, Sunil, Manoj, Ratul, Shobhnath, Karmbeer, Dilip, Kuldeep,
Bhopal, Shiv Prasad, Avinash, Pappu and Ravikant. Moreover, I would like to extend my
thanks to beloved juniors Anuj, Sushmita, Prasann, Krishna, Mahesh, Ajay, Sanjay,
Prudhviraj, Asha, Afjal, Rajesh, Arvind, Jharna, Sadhna, Shivani and Tanupriya.
In addition, I would like to thank to all the non-teaching staff members, Mr.
Neeraj Srivastava, Mr. Faujdar Ram, Mr. Radhey Shyam Ram, Mr. Awadhesh Singh,
Mr. Babu Lal, Mr. Shiv Balak, and Mr. Manoj Sankar Panday of the Department of
Plant Physiology who have been always kind enough to help in their respective roles.
Above and beyond, I have my heartfelt gratitude and reverence to my parents for
their much needed forbearance, utmost patience, considerations and thorough
encouragement in every possible way. They are ever my source of inspiration in this
massive task. I also thank to my lovely sister Vineeta for her love, care and patience during
my whole study period.
Last but not least, I would like to dedicate this thesis to my father Shri. Jadunath
and mother Smt. Jadurani for their immense love, care and understanding throughout my
course of study.

Date:
Place: Varanasi (Dinesh Kumar Yadav)