Monoclonal Antibodies: Hybridomas: A New Dimension in Biological Analyses PDF
Preview Monoclonal Antibodies: Hybridomas: A New Dimension in Biological Analyses
MONOCLONAL ANTIBODIES Hybridomas: A New Dimension in Biological Analyses MONOCLONAL ANTIBODIES Hybridomas: A New Dimension in Biological Analyses Edited by Roger H. Kennett and 1LhonnasJ~cKearn University of Pennsylvania School of Medicine Philadelphia, Pennsylvania and Kathleen B. Bechtol The Wistar Institute Philadelphia, Pennsylvania Plenum Press . New York and London Library of Congress Cataloging in Publication Data Main entry under title: Monoclonal antibodies. Includes index. 1. hnmunoglobulins. 2. Gammopathies, Monoclonal. 3. Cell hybridization. L Kennett, Roger H. IL McKearn, Thomas J. m. Bechtoi,!Kathleen B. [DNLM: 1. Antibodies. 2. Clone cells - hnmunology. QW575 M751] QRI86.7.M66 599.02'93 80-15118 ISBN-13: 978-1-4615-7507-8 e-ISBN-13: 978-1-4615-7505-4 DOl: 10.1007/978-1-4615-7505-4 ©1980 Plenum Press, New York Softcover reprint of the hardcover 1s t edition 1980 A Division of Plenum Publishing Corporation 227 West 17th Street, New York, N.Y. 10011 All rights reserved No part of this book may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher In Memoriam J. WILLIAM MELLMAN Scientist, Pediatrician, and Friend May 7, 1928-February 27, 1980 Contributors C. ABRAMS, Department of Human Genetics, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104 KATHLEEN B. BECHTOL, Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104 YEHUDIT BERGMAN, Howard Hughes Medical Institute Laboratories and Department of Medicine, Stanford University Medical Center, Stanford, California 94305 IRWIN D. BERNSTEIN, Pediatric Oncology Program of the Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, and Department of Pediatrics, University of Washington, Seattle, Washington 98195 PAUL H. BLACK, Department of Medicine, Harvard Medical School, and Massachusetts General Hospital, Boston, Massachusetts 02114 J. ROGER BRIDEAU, MRC Cellular Immunology Unit, Sir William Durtn School of Pathology, University of Oxford, Oxford OXI 3RE, England SHERRI BROWN, Howard Hughes Medical Institute Laboratories and Department of Medicine, Stanford University Medical Center, Stanford, California 94305 W. NEAL BURNETTE, Tumor Virology Program of the Fred Hutchinson Cancer Research Center, Seattle, Washington 98104 M.P. CANCRO, Department of Pathology, University of Pennsylvania, School of Medicine, Philadel phia, Pennsylvania 19104 EDWARD A. CLARK, Department of Genetics, University of Washington, Seattle, Washington 98195 BRIAN CLEVINGER, Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110 M. C. COSEO, Department of Human Genetics, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104 JOSEPH DAVIE, Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110 KATHLEEN DENIS, Department of Human Genetics, University of Pennsylvania, School of Medi cine, Philadelphia, Pennsylvania 19104 BETTY DIAMOND, Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461 vii viii CONTRIBUTORS JEANETTE DILLEY, Howard Hughes Medical Institute Laboratories and Department of Medicine, Stanford University Medical Center, Stanford, California 94305 EDGAR G. ENGLEMAN, Department of Pathology, Stanford University Medical Center, Stanford, California 94305 FRANK W. FITCH, The Committee on Immunology and the Department of Pathology, University of Chicago, Chicago, Illinois 60637 MARK E. FRANKEL, Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104 WALTER GERHARD, Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104 JAMES W. GoDING, Department of Genetics, Stanford University School of Medicine, Stanford, California 94305 RICHARD A. GoLDSBY, Department of Chemistry, University of Maryland, College Park, Maryland 20742 ROGERS GRIFFITH, Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110 J. B. HAAS, Department of Human Genetics, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104 EDGAR HABER, Department of Medicine, Harvard Medical School, and Massachusetts General Hospital, Boston, Massachusetts 02114 DANIEL HANSBURG, Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110 H. HARRIS, Department of Human Genetics, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104 HANS HENGARTNER, Basel Institute for Immunology, CH-4005 Basel5,Switzerland LEONARD A. HERZENBERG, Department of Genetics, Stanford University School of Medicine, Stanford, California 94305 LEROY HOOD, Division of Biology, California Institute of Technology, Pasadena, California 91125 L.L. HOUSTON, Tumor Virology Program or'the Fred Hutchinson Cancer Research Center, Seat tle, Washington 98104 J. C. HOWARD, Department of Pathology, University of Pennsylvania, School of Medicine, Philadel phia, Pennsylvania 19104 ZDENKA L. JONAK, Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104 E. A. JONES, Genetics Laboratory, Department of Biochemistry, University of Oxford, Oxford OXI 3QU, England. Present address: Division of Immunology, Stanford University Medical School, Stanford, California 94305 ROGER H. KENNETT, Department of Human Genetics, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104 DENNIS M. KUNMAN, Department of Pathology, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104 NORMAN KUNMAN, Department of Cellular and Developmental Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037 HILARY KOPROWSKI, Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104 CONTRIBUTORS IX LoIS A. LAMPSON, Department of Anatomy, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104 JEFFREY A. LEDBETTER, Department of Genetics, Stanford University School of Medicine, Stan ford, California 94305 RONALD LEVY, Howard Hughes Medical Institute Laboratories and Department of Medicine, Stanford University Medical Center, Stanford, California 94305 A. DwIGHT LoPES, Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104 MARK E. LOSTROM, Tumor Virology Program of the Fred Hutchinson Cancer Research Center, Seattle, Washington 98104 J. THOMAS McKEARN, Department of Pathology, Divisions of Research Immunology and Labora tory Medicine, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104 BRIAN McMASTER, Pediatric Oncology Program of the Fred Hutchinson Cancer Research Center, Seattle, Washington 98104 W. ROBERT McMASTER, MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, Oxford OXI 3RE, England DAVID H. MARGULIES, Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461 DoNALD W. MASON, MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, Oxford OXI 3RE, England TOMASO MEO, Basel Institute for Immunology, CH-4005 Basel 5, Switzerland CHRISTINE MOLINARO, Department of Cellular and Developmental Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037 EDITH MULLER, Basel Institute for Immunology, CH-4005 Basel 5, Switzerland ROBERT C. NOWINSKI, Tumor Virology Program of the Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, and Department of Microbiology, University of Washington, Seattle, Washington 98195 PAUL V. O'DONNELL, Memorial Sloan-Kettering Cancer Center, New York, New York 10021 BARBARA A. OSBORNE, Department of Genetics, Stanford University Medical Center, Stanford, California 94305 RICHARD A. POLIN, Department of Pediatrics, University of Pennsylvania, School of Medicine, and The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104 MATTHEW D. ScHARFF, Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461 JAMES SCHILLING, Division of Biology, California Institute of Technology, Pasadena, California 91125 LINDA SHERMAN, Department of Cellular and Developmental Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037 C. A. SLAUGHTER, Department of Human Genetics, University of Pennsylvania, School of Medi cine, Philadelphia, Pennsylvania 19104 DAWN E. SMILEK, Department of Pathology, Division of Research Immunology, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104 x CONTRIBUTORS E. SOLOMON, Genetics Laboratory, Department of Biochemistry, University of Oxford, Oxford OXI 3QU, England. Present address: Imperial Cancer Research Fund, P.O. Box 123, Lincoln's Inn Fields, London WC2A 3PX, England TIMOTHY A. SPRINGER, Pathology Department, Harvard Medical School, Boston, Massachusetts 02115 LOUIS STAUDT, Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104 MARY R. STONE, Tumor Virology Program of the Fred Hutchinson Cancer Research Center, Seattle, Washington 98104 MILTON R. TAM, Tumor Virology Program of the Fred Hutchinson Cancer Research Center, Seattle, Washington 98104 TAKESHI TOKUHISA, Department of Genetics, Stanford University School of Medicine, Stanford, California 94305 MICHAEL WEBB, MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, Univer sity of Oxford, Oxford OXI 3RE, England ROBERT A. H. WHITE, MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, Oxford OXI 3RE, England TADEUSZ WIKTOR, Wi star Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104 ALAN F. WILLIAMS, MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, Oxford OX 1 3RE, England DALE E. YELTON, Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461 JONATHAN YEWDELL, Wi star Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104 VINCENT R. ZURAWSKI, JR., Department of Medicine, Harvard Medical School, and Massachusetts General Hospital, Boston, Massachusetts 02114 Preface On August 7, 1975, Kohler and Milstein published in Nature (256:495) a report describing "Continuous cultures of fused cells secreting antibody of predefined specificity." Their report has become a classic and has already had a profound effect on basic and applied research in biology and medicine. By the time the first Workshop on Lymphocyte Hybridomas (Current Topics in Microbiology and Im munology 81, 1978) was held on April 3-5, 1978, in Bethesda, Maryland, investi gators from many laboratories had made hybrids between plasmacytomas and spleen cells from immunized animals and had obtained monoclonal antibodies reacting with a broad variety of antigenic determinants. At the time Kohler and Milstein introduced this new technology, the editors of this volume were involved in the production of antisera against differentiation antigens (K.B.B.), histocompatibility antigens (T.]. McK.), and human tumor associated antigens (R.H.K.). Because of the potential usefulness of monoclonal antibodies in these areas, we each began production of hybridomas and analysis of the resulting monoclonal reagents. One of the most interesting aspects of participation in the early stages of the development and application of hybrid oma technology has been observing how the implications of the initial observa tions gradually spread first among the practitioners of immunology and immu nogenetics, and then to other areas of the biological sciences, such as developmental biology, biochemistry, human genetics, and cell and tumor biology. Besides providing a novel way of obtaining homogeneous antibodies that will be useful in many areas of biology, Kohler and Milstein's achievement also provides a prototype for the use of hybrid cells to "trap" clones of cells perform ing a specialized function as transformed cell lines producing a specific product or performing a specific function in vitro. When one considers that the produc tion of specific immunoglobulins may be only the first of several such functions that could be "immortalized" by cell fusion, then it appears likely that the full implications of Kohler and Milstein's report may still be far from full realization. This volume is designed to present an overview of the production and uses xi Xli PREFACE of monoclonal antibodies and to facilitate their application to other areas of biology. It is intended for advanced undergraduates, graduate students, and scientists in the biological and medical sciences. We do not presume to consider the volume comprehensive, but have attempted to include examples of many of the approaches and applications that have been developed to exploit this technology . Roger H. Kennett J. Thomas McKearn Kathleen B. Bechtol Philadelphia
Description:The list of books you might like
