Molecular Cell Biology (Lodish, Sixth Edition)

1265 Pages·2007·181.93 MB·English

by  Harvey Lodish

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About the cover: Mitotic PtK2 cellsi n late anaphases tainedb lue for DNA and greenf or tubulin. Courtesyo f Torsten\ gittman.
Library of CongressC ataloging-in-PublicationD ata
Molecular cell biology I Harvey Lodish . . . [et al.]. -6th ed.
p. cm.
Includesb ibliographicalr eferencesa nd index.
1. Cytology. 2. Molecular biology. I. Lodish, Harvey F.
QH581.2.M6552 007
57L5-dc22 2007006188
ISBN-13 : 978 -0-71 67- 76 01-7
ISBN-10:0 -71 67- 7 601-4
@ 1986,1.990,1 995,2000,2004,2008 by tJf.H . Freemana nd Company
All rights reserved.
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To our studentsa nd to our teachers,
from whom we continuet o learn,
and to our familiesf,o r their support,
encouragemenat,n d love

PREFACE
n writing the sixth edition of Molecular Cell Biology what we know. A number of experimental organisms,f rom
I
we have incorporated many of the spectaculara dvances yeastst o worms to mice, are used throughout so the student
I
I made over the past four years in biomedical science,d riven can seeh ow discoveriesm ade with a "lower organism" can
in part by new experimental technologiest hat have revolu- Iead directly to insights even about human biology and dis-
tionized many fields. High-velocity techniquesf or sequenc- ease.T his experimental approach, evident in the text itself,
ing DNA, for example,h aveg eneratedt he completes equence has also been thoroughly integrated into the pedagogical
of dozens of eukaryotic genomes;t hese in turn have led to framework. For example:
important discoveriesa bout the organization of the human
genome and regulation of gene expression,a s well as novel r Experimental Figures lead students through important
insights into the evolution of life-forms and the functions of experimental results.
individual members of multiprotein families. New imaging
techniquesh ave generatedp rofound revelations about cell r Classic Experiments essaysf ocus on historically impor-
organization and movement, and new molecular structures tant and Nobel Prize-winninge xperiments.
have greatly increasedo ur understanding of life processes
r New and revised Analyzet he Data problems at the end
such as cell-cell signaling, photosynthesis,g ene transcrip-
of each chapter require the student to synthesizer eal ex-
tion, and chromatin structure.
perimental data to answer a serieso f questions.
New Author Team r Updated Perspectives for the Future essays explore
potential applications of future discoveriesa nd unanswered
Two new authors have been instrumental in refocusing this questionst hat lie ahead in research.
book toward these exciting new developments. Anthony
Bretscher of Cornell University is known for identifying and
characterizing new components of the actin cytoskeleton and
elucidating their biological functions in relation to cell polarity
and membrane traffic. Hidde Ploegh, of the Massachusetts
Institute of Technologg has made major contributions to our
understanding of immune system behavior, particularly in
regard to the various tactics that viruses employ to evade our
immune responsesa nd the ways our immune systemr esponds.
Both authors are widely recognized for their researcha s well
as their classroomt eachinga bilities.
'We
are grateful to Paul Matsudaira, Jim Darnell, Larry
ZipurskS and David Baltimore for their exceptional contri-
butions to the previous editions of Molecular Cell Biology.
Much of their vision and insight is apparent at many places
in this book.
ExperimentaEl mphasis
Fluorescenmceic roscopsyh owst he locationo f DNAa nd multiple
The hallmark of Molecular Cell Biology has always beent he proteinws ithint he samec ell.l FromB N G Giepmaents a l, 2006S, cience
use of experimentst o teach students how we have learned 3'12:21|7
vtl
New DiscoveriesN,e w Methodologies
Coiled-coisl talk
Methodological advances continue to expand and enrich
our knowledge of molecular cell biology and lead to new
understanding. Following are just a selection of the new
Motor head
experimental methodologiesa nd cutting-edges ciencei ntro-
Neckl inkers Microtubule (+) duced in this edition:
r Expanded coverage of proteomics, including organelle
proteome profiling and advances in mass spectroscopy
(Chapter3 )
I Forwardm otorb indsB -tubulin,
^ releasinAgD P r Expandedc overageo f RNAi, including the useo f shRNAs
to inhibit any gene of interest in a cultured cell or organism
(Chapters5 , 8)
r Updated discussionso f chromatin, including structure and
condensation( Chapter6 ), control of genee xpressionb y chro-
matin remodeling (Chapter 71, and chromatin-remodeling
p Forwardh eadb indsA TP proteins and tumor development( Chapter 25)
?
r Evolution of chromosomes and the mitochondrion
(Chapter 6)
r New molecular models, including pre-initiation complex
and mediator complex (Chapter 7); annular phospholipids
(Chapter 1,0);C az* ATPase( Chapter 11); rhodopsin, trans-
p Conformationacl hangei n
neckl inkerc ausesr ear ducin, and protein kinase A (Chapter 15); and myosin
head to swing forward ATPase (Chapter 17)
r Latest advancesi n light and electron microscopy,i nclud-
ing cryoelectron tomography (Chapter 9)
r Reactive oxygen species( ROS) (Chapter 12)
[ rue* forwardh eadr eleaseAs DB
^ trailingh eadh ydrolyzesATP r Role of supercomplexesi n electron transport (Chapter 12)
V andr eleasePs .
r Human epidermal growth factor receptors (HERs) and
treatment of cancer (Chapter 16)
r Myosin ATPasec ycle (Chapter 17)
r Kinesin-1M Pase cycle (Chapter 18)
r Use of retrovirus infection for tracing cell lineage
(Chapter2 1)
r Axon guidance molecules (Chapter 23)
r Somaticg ener earrangementi n immune cells (Chapter 24)
r Cancers tem cells (Chapter2 5)
r Use of DNA microarray analysis in tumor typing
(Chapter2 5)
Figure1 8-22K inesin-u1s esA TPt o "walk"d owna microtubule
vill PREFACE
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