Molecular Biology of Hematopoiesis 5

696 Pages·1996·19.238 MB·English

by  Richard Champlin, Sergio Giralt, James Gajewski (auth.), Nader G. Abraham, Shigetaka Asano, Günther Brittinger, Georges J. M. Maestroni, Richard K. Shadduck (eds.)

Molecular Biology of
Hematopoiesis 5
Molecular Biology of
Helllatopoiesis 5
Edited by
Nader G. Abraham
The Rockefeller University
New York, New York
Shigetaka Asano
University of Tokyo
Tokyo, Japan
Gunther Brittinger
Universitiit-Gesamthoschschule Essen
Essen, Germany
Georges J. M. Maestroni
Istituto Cantonale di Patologia
Locarno, Switzerland
and
Richard K. Shadduck
Western Pennsylvania Cancer Institute
Pittsburgh, Pennsylvania
Plenum Press • New York and London
L1brary of Congress Catalog1ng-1n-PubI1cat1on Data
Molecular biology of hematopoiesis 5 I edited by Nader G. Abraham ...
[eta1.l.
p. cm.
"Proceedings of the Ninth Symposium on the Molecular Biology of
Hematopoiesis. held June 23-27, 1995, in Genoa, Italy"--T.p. verso.
Inc 1u des bib 1 i ograph i ca 1 references and index.
1. Myeloproliferative disorders--Molecular aspects--Congresses.
2. Hematopoietic stem cell disorders--Molecular aspects--Congresses.
3. Hematopoiesis--Congresses. 4. Bone marrow--Transplantation-
-Molecular aspects--Congresses. I. Abraham, Nader G.
II. Symposium on the Molecular Biology of Hematopoiesis (9th 1995
Genoa, Italy)
[DNLM: 1. Hematopoiesis--congresses. ~H 140 M7177 1996]
RC645.75.M65 1996
616.4' 107--dc20
DNLM/DLC
for Library of Congress 96-27564
CIP
Proceedings of the Ninth Symposium on the Molecular Biology of Hematopoiesis,
held June 23 -27,1995, in Genoa, Italy
ISBN-13: 978-1-4613-8031-3 e-ISBN-13: 978-1-4613-0391-6
DOl: 10.1007/978-1-4613-0391-6
© 1996 Plenum Press, New York
Softcover reprint of the hardcover I st edition 1996
A Division of Plenum Publishing Corporation
233 Spring Street, New York, N. Y. 10013
All rights reserved
1098765432 I
No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any
means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written
permission from the Publisher
Volumes 1-4 were published by Intercept, Ltd., Andover, United Kingdom
PREFACE
This volume of Molecular Biology ofH ematopoiesis is dedicated to John W. Adam
son, M.D., Tadamitsu Kishimoto, M.D., Robert C. Gallo, M.D., Arthur W. Nienhuis, M.D.,
and Franco Mandelli, M.D., for their contributions in developing an overall view of the
state-of-the-art knowledge in the field of hematopoiesis. Richard Champlin, among other
renowned clinicians, presented updated information on stem cells and T-cell depletion for
bone marrow transplant. A clinical update on thrombopoietin was presented by Pamela Hunt
of Amgen and by Kenneth Kaushansky. Arthur Nienhuis' and Katherine Turner's contribu
tions to our current knowledge and advances in the fields of growth factors and gene transfer
were also recognized during the 9th Symposium on Molecular Biology of Hematopoiesis in
Genoa. The chapters cover such diverse areas as preclinical and clinical updates on growth
factors and positive and negative regulatory molecules. "Advances in Leukemia: Mechanism
and Treatment by Interferon" was presented by Professor Sante Tura. Readers will find
presentation of exciting advances that have occurred in the area of hematopoiesis. The
elucidation of gene structures of key growth factor proteins such as IL-12 and IL-II will
lead to new insights and new approaches in understanding the regulation of hematopoiesis,
as well as application of new growth factors.
Retrovirus gene transfer techniques and genetic manipulation of murine and human
cells have added new information on the regulatory steps involved in cell development and
genetic disorders. Transfection of eukaryotic cells, specifically bone marrow cells and,
subsequently, in vivo transplantation, further suggest that hematopoietic reconstitution is
possible. Studies of gene transfer continue to reveal the mechanism behind various biological
events in which normal and abnormal cell proliferation and differentiation will be achieved.
Further, origination and isolation of stem cells, as well as the use of cord blood for bone
marrow transplant, were presented. Enthusiasm for the discovery of new growth factor( s)
and interleukin(s) remains strong as novel factors are introduced into the field and presented
at the meeting. Also, exciting data are being generated in linking growth factors and
neuroendocrines to proto-oncogenes and cell proliferation and differentiation. More data
will continue to accrue, in particular, with respect to the cell surface receptors, the mecha
nisms of shut-off of local production of these factors, and possible clinical side effects of
their continued administration. We are convinced that this volume will be a useful reference
for clinicians, scientists, and investigators who are interested in the field of hematopoiesis.
Nader G. Abraham
v
ACKNOWLEDGMENTS
The Organizing Committee wishes to acknowledge the Menarini International Foun-
dation and the following companies, whose support made this meeting possible:
• Amgen-Roche, SpA
• Chugai Rhone-Poulenc
• Ortho Biotech
• CellPro Inc., Europe N.V'/S.A.
• Schering Plough SpA
• Wellcome Italia SpA
• Glaxo Group Research Limited
• Baxter Healthcare Corporation
• Miltenyi Biotec
• Schering Plough International
• Blackwell Science
• Le Petit (Marion-Merrell Dow)
• Impress
• Stem Cell
• SyStemix
• Ortho Biotech Inc.
• Beckman
• Sandoz
• Immunokontact
• Vestar
• Liposome
• Stockton
• Pasteur Merieux
• Fondaz. Int. Menarini
.OPA
• Intercept
• Plenum
The Organizing Committee gratefully acknowledges the patience and support, in
cluding editorial assistance, in preparation of this conference of Joyce Eshet, Symposium
Coordinator.
vii
viii Acknowledgments
For further infonnation, please write to:
N.G. Abraham
New York University School of Medicine
Brooklyn Hospital Campus
Brooklyn, NY 11201
or
The Rockefeller University
1230 York Avenue
New York, NY 10021
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CONTENTS
Part I. Advances in Bone Marrow Transplant:
Stem Cells and T-Cell Depletion
1. T-Cells, Graft-versus-Host Disease, and Graft-versus-Leukemia: New
Approaches for Blood and Marrow Transplantation .................. .
Richard Champlin, Sergio Giralt, and James Gajewski
2. Combined Use of Growth Factors to Stimulate the Proliferation of
Hematopoietic Progenitor Cells after Autologous Bone Marrow
Transplantation (ABMT) for Lymphoma Patients . . . . . . . . . . . . . . . . . . . .. II
Roberto M. Lemoli, Alessandra Fortuna, Miriam Fogli, Gianantonio Rosti,
Filippo Gherlinzoni, Giuseppe Visani, Lucia Catani, Alessandro Gozzetti,
and Sante Tura
3. Long-Term Multilineage Development from Human Umbilical Cord Blood
Stem Cells in a Novel SCID-hu Graft .............................. 21
Christopher C. Fraser, Hideto Kaneshima, Gun Hansteen,
Madison Kilpatrick, Ronald Hoffman, and Benjamin P. Chen
4. Peripheral Blood Stem Cell Mobilization with G-CSF (Filgrastim) Alone for
Autologous Transplant in Myeloid and Lymphoid Malignancies ......... 31
E. Archimbaud, M. Michallet, I. Philip, C. Sebban, P. Tremisi, G. Clapisson,
A. Belhabri, and D. Fiere
5. Density Separation and Cryopreservation of Umbilical Cord Blood Cells:
Evaluation of Recovery by Means of Short-and Long-Term Culture 37
Camillo Almici, Carmelo Carlo-Stella, John E. Wagner, and Vittorio Rizzoli
6. Economic Implications of High Dose Chemotherapy Programs with
Autologous Stem Cell Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 45
Charles L. Bennett, Michael R. Bishop, and Subhash C. Gulati
ix
x Contents
7. CFU-GM Growth from Peripheral Blood Stem Cells (PBSC) before and after
Cryopreservation: Comparison with Bone Marrow Cells ............... 53
M. Bonfichi, C. Brera, A. Balduini, E. P. Alessandrino, P. Bernasconi,
D. Troletti, M. Boni, C. Castagnola, E. Brusamolino, G. Pagnucco,
C. Perotti, L. Salvaneschi, and C. Bernasconi
8. Is There an Embryological Basis for the Association of Mediastinal Germ Cell
Tumors and Hematologic Cancers? A Review . . . . . . . . . . . . . . . . . . . . . . .. 59
William J. Larsen
9. Autologous BMT for Treatment of Experimental Autoimmune Diseases ....... 69
D. W. van Bekkum and M. van Gelder
10. Peripheral Blood Precursor Cell Transplants across a Major Histocompatibility
Barrier in Rabbits: Positive Effect of a Higher Number of Precursor Cells? 77
A. Gratwohl, H. Baldomero, L. John, A. Tichelli, A. Filipowicz, C. Nissen,
and B. Speck
II. Reticulocyte Parameters as Early Indicators of Hematopoietic Recovery after
Bone Marrow Transplantation .................................... 83
M. Tommasi, G. d'Onofrio, G. Zini, P. Salutari, S. Sica, and G. Leone
12. Primordial Germ Cell-Derived Hemopoiesis: A New Concept for the Initiation
of Hemopoiesis ................................................ 93
Ivan N. Rich and Frank Zimmermann
13. Monitoring of Serum HLA Class I Antigen Levels in Allogeneic Bone Marrow
Transplantation ................................................ 101
Francesco Puppo, Sabrina Brenci, Massimo Ghio, Donatella Bignardi,
Paola Contini, Andrea Bacigalupo, Maria T. Van Lint, Gilberto Filaci,
Marco Scudeletti, Soldano Ferrone, and Francesco Indiveri
14. Correlation of ELISA-Measured Anti-HLA Class I IgG and IgG I Antibodies
and First-Year Rejection Episodes ................................. 107
Jeffrey Regan, Francisco Monteiro, Daniel Speiser, Jorge Kalil,
Ronald Kerman, Philippe Pouletty, and Roland Buelow
15. Inactivation or Down-Regulation of the Overstimulated, HLA-DR-(CD2R-,
CD26-, CD38- and/or CD69-) Positive T Cell Subset Enables the
Immunocompetent CeIls to Restore the Pre-Disease State: A Novel
Technique ("Microimmunosurgery"), Based on a GvHD-Free GvLlGvT
Effect, AIlows a Complete (100%) Eradication of Solid Tumors by an
Indirect Attack on the Tumor-Protecting CD8+ HLA-DR+ Cells .......... lIS
P. Leskovar and I. Vodenitcharov
16. European Cup Trial: A Randomized Trial Comparing the Efficacy of
Chemotherapy with Purged or Unpurged Autologous Transplantation in
Adults with Poor Risk Relapsed Follicular NHL: An EBMT Working
Party Trial .................................................... 123
A. PorceIlini, H. Schouten, B. Stade, and R. KoIl
Contents xi
Part II. Clinical Update on Malignant Lymphoma and Myeloma:
Mechanism/Therapy
17. Mechanisms of Chromosomal Translocations in Malignant Lymphomas ....... 127
Michael J. Uppenkamp, Heinz-Gert HOffkes, Peter Meusers, and
Gunter Brittinger
18. Molecular Findings and Classification of Malignant Lymphomas ............. 135
Stefano A. Pileri, Claudio Ceccarelli, Elena Sabattini, Donatella Santini,
Omella Leone, Stefania Damiani, Lorenzo Leoncini, and
Brunangelo Falini
19. Mantle Cell Lymphoma: Genetic Lesions and Their Role in Classification and
Progression ................................................... 145
G. Ott, M. M. Ott, J. Kalla, A. Helbing, B. Schryen, T. Katzenberger,
J. Bartek, A. Durr, J. G. Muller, H. Kreipe, and H. K. Muller-Hermelink
20. Lymphoblastic Lymphoma in Adults .................................... 151
John Sweetenham
21. A New Non-Radioactive Method for Detection of Monoclonal Cell Populations
in Patients with Burkitt's Lymphoma ............................... 159
Udo zur Stadt, Alfred Reiter, Jorg Tomeczkowski, and Karl-Walter Sykora
22. Oncogenes in the Pathogenesis of Multiple Myeloma ...................... 169
Paolo Corradini, Monica Astolfi, Marco Ladetto, Silvia Campana,
Mario Boccadoro, and Alessandro Pileri
23. Expression and Regulation of c-kit Receptor and Proliferative Response to
Stem Cell Factor in Childhood Lymphoma and Leukemia Cells ......... 173
J. Tomeczkowski, D. Frick, B. Schwinzer, A. Reiter, K. Welte, and
K. W. Sykora
24. Growth Inhibition in IL-7 Receptor Positive Childhood Burkitt's Lymphoma
Cells Is Not Due to Induction of Apoptosis .......................... 191
Karl W. Sykora, Jorg Tomeczkowski, Konstanze Kirchhoff, ElifYakisan,
Alfred Reiter, and Karl Welte
Part III. Update on Advancement in Leukemia
25. Expression of the Multidrug-Resistance Gene Product, P-Glycoprotein, in
Adult Acute Myeloid Leukemia: Association with Stem Cell Antigen
CD34 and Specific Cytogenetic Abnormalities ....................... 201
Elisabeth Paietta, Janet Andersen, Jorge Yunis, Jacob M. Rowe,
Peter A. Cassileth, and Peter H. Wiernik,
for the Eastern Cooperative Oncology Group
26. Characterization ofN-RAS Promoter Mutations in Leukemia ................ 207
Harry Hand, Jacqui Thorn, and Peter Molloy
xii Contents
27. The Role of Anthracyclines in the Management of Adult Acute Lymphoblastic
Leukaemia .................................................... 215
R. Bassan, T. Lerede, A. Rambaldi, E. Di Bona, G. Rossi, E. Pogliani,
G. Lambertenghi-Deliliers, A. Porcellini, P. Coser, and T. Barbui
28. Collection ofbcr-abl Negative Peripheral Blood Progenitor Cells in Newly
Diagnosed Patients with Chronic Myeloid Leukaemia ................. 221
N. Storey, A. L. Lennard, A. M. Dickinson, J. A. Irving, D. Rowe,
D. Levett, J. Dunn, A. R. Cattan, and S. J. Proctor
29. CD34 Quantitative Flow Cytometry Study of the Blastic Population in Acute
Leukemias .................................................... 227
Gian Matte<;l Rigolin, Francesco Lanza, and Gianluigi Castoldi
30. Cytokine Gene Expression in a Case ofB-Cell Chronic Lymphocytic Leukemia
(B-CLL) with an Unusual Expansion ofT Cells at Presentation .......... 237
Athanasia Mouzaki, Vincent Kindler, Nicolette Bowers, Arlette Doucet,
Maria Melachrinou, Maria-Christina Kyrtsonis, and
Alice Kallinikou-Maniatis
31. Exploitation of Frequent p 16 Deletion in the Treatment of T Cell Acute
Lymphoblastic Leukemia ........................................ 247
A. L. Yu, J. Chen, M. B. Diccianni, A. Batova, and J. Yu
32. Detection ofbcr-abl m-RNA in Single Progenitor Colonies by PCR:
Comparison with Cytogenetics and PCR from Uncultured Cells ......... 257
Elisabeth Schulze, Rainer Krahl, Karin Thalmeier, and Werner Helbig
33. Quantitative RT-PCR ofPML-RARa Transcripts in Acute Promyelocytic
Leukemia ..................................................... 269
Harry Hand, Francisca Springall, Tao Zeng, and Katrina Bradfield
Part IV. Update on Role of HIV and Viruses in Hematopoiesis
34. AIDS-Related Non-Hodgkin Lymphomas: Molecular Genetics, Viral Infection,
and Cytokine Deregulation ....................................... 277
Gianluca Gaidano, Cristina Pastore, Annunziata Gloghini, Daniela Capello,
Gisella Volpe, Paolo Ghia, Giuseppe Saglio, and Antonino Carbone
35. Anti-HIV Viral Interference Induced by Retroviral Vectors Expressing a
Non-Producer HIV-I Variant ...................................... 285
M. Federico, R. Bona, P. D'Aloja, M. Baiocchi, K. Pugliese, F. Nappi,
C. Chelucci, F. Mavilio, and P. Verani
36. Suppression of Hematopoietic Support Function Is Associated with
Over-Expression ofIL-4 and TGF~I in LP-BM5 MuLV Infected Stromal
Cell Lines ..................................................... 293
Vincent S. Gallicchio, Kam-Fai Tse, Jennifer Morrow, and Nedda K. Hughes