Molecular barcoding and larval gut content analysis in insects (Geometridae, Lepidoptera)

2006·1 MB·

by  Axel Hausmann

GBIF-D: Geometridae: FORUM HERBULOT:
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&sid=T&only=no&syno=n&valid=n&lang=d
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Staude,H.S.,A.Coetzer,B.Coetzer,D.M.Kroon,J.Joannou&M.Krüger(2006):
TheLepiafricaLivingBooksProject.-Spixiana29/3:210
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formationand ImagesofasmanyaspossibleAfro- Lepidops® database prograni already in use by
tropical Lepidoptera in an easy to use structured members of The Lepidopterists' Society of Africa.
electronic formattointerested parties. Lepidops®is economical, effectiveand easy to use.
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Molecularbarcodingand larvalgut contentanalysis in insects
(Geometridae, Lepidoptera)
AxelHausmann, Michael A. Miller& GünterC. Müller
Hausmann, A., M. A. Miller & G. C. Müller (2006): Molecular barcoding and
larvalgutcontentanalysisininsects (Geometridae,Lepidoptera). -Spixiana29/3:
210-211
Corresponding author: Dr. Axel Hausmann, Zoologische Staatssammlung
München,Münchhausenstr. 21,D-81247München,Germany;
e-mail: [email protected]
Onthebackgroundoftheenormousspeciesnumbers sity.Itmay,however,alsogainacertainimportance
in insects, the innovative technique of molecular forecosystemresearch, and systematics.
barcodingwill more anci more playa majorrole in Intheyearof2005theZSMhasgotofferedaccess
entomologicalresearchbyfacilitatingIdentification to several thousands of neotropical Geometridae
of all stages, and thus for assessment of biodiver- larvae coUected in 1800 fogging samples of Terry
210
Erwin (Lucky et al. 2002; Erwin et al. 2006), who tion on host-plant relationships and host specifity
monitoredthefaunaof200treesin9replicatesfrom for a large group of phytophagous insects in the
1994-1996 in north-eastern Ecuador. Identity of all neotropical rain forest canopy. With thesedata the
thefogged trees,andtheirneighboursisknown. In estimationsoftotalspeciesnumbersinGeometridae
twopilotstudieswecouldprove,thatlarvaecanbe andinsectsmaybeextrapolatedandrefined.Similar
identified to species by their 'barcode sequences" projects are planned for geometrid moth larvae in
(mtDNA), and that sequencing of gut content is Israel.
possibletoo, in orderidentify the larvalplantmeal
andtoprovefeedingonthefoggedhost-tree,rather
than on epiphytes or on the neighbouring tree References
(Milleretal. 2006; Mathesonetal. 2006). Identifica-
tionofthelarvaewas performedbyanalysisofthe Erwin,T. L., M. C. Pimienta,O. E. Murillo & V. Asche-
completesequenceofthemitochondrial genecyto- ro2006. Mapping Patternsof ß-Diversity for Beet-
chrome c oxidase I (COI) and comparison with se- lneasryAcCraosses tfohreIWmepsrtoevrinnAgmIanzvoenntoBraysiMn:etAhoPdrseliamnid-
quences of coUection specimens. The effectiveness
ConservationStrategies
ofthe 'barcoding' toolforspeciesIdentificationhad Hebert, P. & A. Mitchell 2006. DNAbarcoding ofAus-
already been shown in many other studies (cf e.g. tralian Lepidoptera. -Spixiana 29(3): 211-212
Hebert&Mitchell2006).Gutcontentsweresuccess- Lucky, A., T. L. Erwin &J. D. Witman 2002. Temporal
fullyidentifiedby comparing sequence ofa 157bp and spatial diversity and distribution of arboreal
longfragmentofthechloroplastgenerbcLwiththat Carabidae (Coleoptera) in a western Amazonian
ofofthepre-identifiedhost-plantandawidesetof rain forest. - Biotropica 34(3): 376-386
otherplantsofthestudyarea. Plantmealscouldbe Matheson, C. D., G. Müller, K. Vernon, A. Junnila, A.
detected,whentheinsectswerekilledandpreserved Hausmann,M.A.Miller,C.Greenblatt&Y.Schiein
i(nMaEtthheasnoonleutpal.tosu1b2mihtoteudr)s.aFfotrerlatrhgeelsaesttsfoefedpionsg- f(souubnmdititnetdh)e.gSuptecoifeisnIsdeecnttsibfiycaatPioCnRofmepltahnotdr.e-siOdDueEs
(submitted)
sible host-plants and for discrimination of closely Miller, M. A., G. C. Müller, V. D. Kravchenko, A. Jun-
related plant species, e.g. in tropical countries, addi- nila,K.K.Vernon,C.D.Matheson&A.Hausmann
tionalmarkers(fragments/genes)maybenecessary. (inprint). DNA-based Identification ofLepidopte-
Results from the planned research project will ra larvae and plant meals from their gut content.
provide, forthefirsttime, comprehensiveInforma- -Russ. Ent.
J.
DNA
barcoding ofAustralian Lepidoptera
PaulHebert& AndrewMitchell
Hebert, P. & A. Mitchell (2006): DNA barcoding of Australian Lepidoptera. -
Spixiana29/3:211-212
Correspondingauthor: Dr.AndrewMitchell,AgriculturalScientificCollections
Unit,OrangeAgriculturalInstitute,NSWDepartmentofPrimaryIndustries,Forest
Rd,OrangeNSW2800,Australia; e-mail: [email protected]
DNAbarcodes are short (658bp) sequences from a landandtheCentralWestofNewSouthWales.All
standardized region of the mitochondrial gene cy- specimensweredatabasedandphotographedbefore
tochrome c oxidase I (COI or coxl). Fast work has DNAwasextractedfromaSingleleg.DNAbarcodes
revealedthatsequencediversityinthisgeneregion weresubsequentlygatheredfromthespecimensand
is an effective tool for species Identification and analysed using the Barcode of Life Data System
discovery. As a result, large-scale DNA barcoding (www.barcodinglife.org)
.
programs are now underway, including efforts to Levelsofintra-specificVariationatCOIaveraged
assemble barcodes for all fish and all bird species. just0.2 %,whilecongenericspeciesshowedsequence
We intend to develop a comprehensivebarcode li- divergencesthatwere,onaverage,20timeshigher.
braryforAustralianlepidopteransasacomplement As with studies in other geographic regions, more
toa similarprojectunderwayinNorthAmerica. than95 %ofthespeciesthatweexaminedpossessed
Wenowpresentresultsofapilotstudythathas uniqueDNAbarcodes,allowingtheireasyIdentifi-
barcoded 3500 specimens representing over 800 cation. Although therewas littleoverlap inspecies
speciescollectedfromsitesinnorth-easternQueens- coverage between our two sampling regions, our
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