Table Of Content

CONTENTS OF VOLUME 23 Number 1/2 BIOLOGICAL APPLICATIONS M. A. HAYAT Quantitation of immunogold labeling S. DEy Differential distribution of some elements in a camera type eye-lens as revealed by energy dispersive X-ray micro-analysis M. TZAPHLIDOU, A study of staining for electron microscopy C. CUMMINGS and using collagen as a model system—IX. The J. A. CHAPMAN effect of tannic acid fixation F. VERNI, G. ROSATI, Morphological relationship between parafla- P. LENzI, L. BARSANTI, gellar swelling and paraxial rod in Euglena V. PASSARELLI and gracilis P. GUALTIERI BIOLOGICAL TECHNIQUES K. KANAYA, Y. MURANAKA, Quantitative analysis of sputtering due to ion K. YONEHARA and K. ADACHI beam bombardment of solids and biological specimens in high resolution electron microscopy EXTENDED ABSTRACTS OF THE ANNUAL MEETING OF THE SCANDINAVIAN SOCIETY FOR ELECTRON MICROSCOPY E. BEHALOVA, P. HYTTEL, 69 The block against polyspermic fertilization in S. SMITH, T. GREVE and cattle H. CALLESEN H. Birn, E. I. CHRISTENSEN The dynamics of membrane transfer during and S. NIELSEN endocytosis and membrane recycling in renal proximal tubule W. M. Businac, J. M. BROCK New developments in electron microscopy for and M. T. OTTEN life science L. M. Cruz-ORIVE, Characterizing anisotropy: a new concept L. M. KaRLsson, S. E. LARSEN and F. WAINSCHTEIN SHIYING Cul and Serial section analysis of the apical part of E. I. CHRISTENSEN renal proximal tubule cells V. DANTZER and R. LEISER Areola-gland subunits in the epitheliochorial types of placentae from horse and pig A. C. ENDERS Contributions of electron microscopy to our understanding of implantation and cell layer formation in primates B.-A. FREDRIKSSON and Freeze-drying and cryosputtering with tung- M. LINDROTH sten or niobium for high resolution scanning electron microscopy L A. GINSEL and Routing of brushborder and _ lysosomal J A. M. FRANSEN enzymes in polarized epithelial cells (review) G . H. HANSEN, Microvillus membrane enzymes along the L .-L. WETTERBERG, crypt—villus axis M. D. POULSEN, O. NOREN and H. SJOsTROM S. H. HANSEN Early endocytic events W. K. HENEEN, G. KARLSSON, The ultrastructure of lipid-rich oats K. BRISMAR and B. MATTSSON E. I. CHRISTENSEN Membrane receptors involved in endocytosis in rat renal proximal tubule M. JARVILEHTO and Actin filament array in an interstitial space is R. HARJULA disorganized differently by capping agents CaCl, and KI L. KAYSER Quantitative dynamic fluorescence microscopy R. F. KLINGE The fine structure of the mandibular condyle V. Kopecny Specific features of bovine embryonic nucleo- logenesis: a review of fine-structure autoradio- graphic and immunoelectron microscope studies N. M. LEE, C. WEIBULL, Microscopical studies of phosphorus accumu- E. CARLEMAN and lating bacteria in sludge from waste water T. WELANDER treatment plants J: LINDEN, R. POHJANVIRTA, The effect of TCDD on the pineal gland of T. RAHKO and J. TuoMISsTo Han/Wistar rats S. MARTTILA, I. PORALI and Expression of different acid proteinases in A. MIKKONEN germinating barley seed A. B. MAUNSBACH and Ultrastructural immunolocalization of Na,K- F. P. REINHOLT ATPase in high-pressure frozen kidney tubules T. Moos and Y. BALSLEV Retrograde axonal transport of albumin-—gold complex in rat motor neurons: a light and electron microscopic study R. G. PARTON, K. SIMONS and Axonal and dendritic endocytic pathways in C. G. Dotti cultured neurons iv K. PIHAKASKI- MAUNSBACH Endocytosis in cold acclimated and non- and T. PUHAKAINEN acclimated protoplasts E.-L. PUNNONEN, S. AUTIO Effect of tunicamycin on autophagy in cul- and H. REUNANEN tured rat fibroblasts K. ROBERG and Visualization of iron in cultured macrophages J. M. ZDOLSEK with autometalography S. SAARI, S. NIKANDER and A SEM study of the morphological features of T. RAHKO the lower respiratory tract epithelium of the reindeer (Rangifer tarandus tarandus L.) M. SHAMSUDDIN, B. LARSSON, Ultrastructure of in vivo and in vitro developed H. GUSTAFSSON and bovine embryos H. RODRIGUEZ-MARTINEZ F. VENNIGERHOLZ, B. WALLES Immunocytochemical localization of pectin in and A. GEITMANN stylar tissues A. J. VERKLEW Immunogold labeling in cell biology P. WALLEN and Confocal laser scanning microscopy as a tool O. SHUPLIAKOV to study the 3-D structure of identified neurons V. ALFREDSSON HREM study of zeolite Na-Y S. J. ANDERSEN, O. JENSRUD, Si-particles in an AINiSiMn alloy I. JOHANSEN, H. J. ROVEN and R. HIER L. N. BAKKEN, Many-parameter refinements from CBED K. MARTHINSEN, R. H@IER pattern and R. HOLMESTAD A. BARDAL, M. ZWERGER and YBa,Cu,O, _; thin films on Si substrates with O. EIBL Y,0O, and (Zr,_,Y,)O, buffer layers. The YBa,Cu,O,_;-Y,0, and Y,O,-(Zr, _,.Y,)O, interfaces M. D. BENTZON, A sensitive way to determine the thickness of S. S. ESKILDSEN and thin carbon films by SEM P. S. NIELSEN J.-O. BovIN Elemental mapping with EDX on a used automobile catalytic converter J.-O. BovIN On the determination of the oxidation states of iron with PEELS using a standard TEM J.-O. Bovin, A. CARLSSON, On the structure and temperature stability of J.-O. MALM and G. SCHMID ligand covered, 18 nm, gold colloids J. M. Brock, M. T. OTTEN High-resolution imaging with a coherent and W. M. CoENE electron source: the field emission gun J. M. Brock, M. T. OTTEN Improved spatial resolution in imaging, dif- and M. J. C. DE JONG fraction, microanalysis and scanning with a field emission TEM/STEM A. CARLSSON, R. NORRESTAM On the cation disorder in _pinakiolite, and J.-O. BOVIN (Mg,Mn),0,BO, Y. F. CHENG, V. HANSEN, TEM study of the secondary particles in strip- J. GI@NNES and cast Al3003 alloys L. R. WALLENBERG C. CLAUSEN and Observation of voltage contrast at grain J. B. BiLDE-S@RENSEN boundaries in YSZ R. W. ERIKSEN, On-line acquisition of electron diffraction E. SORBRODEN, S. MESSELT data in TEM and A. OLSEN P. J. FALLON and L. M. Analysis of CVD diamond using TEM and BROWN parallel EELS in a STEM L. GERWARD X-ray attenuation coefficients: current status and availability Y. X. Guo, S. J. ANDERSEN, A TEM study of a newly discovered meta- H. K . NYLUND and R. H@IER stable phase in an AlMnCrSi alloy N. N . HAMAWI and Nucleation of TiN on tungsten needles K.G LEJBOL P. L. HANSEN, D. McComs ELNES fingerprint of Al coordination in and R. BRYDSON nesosilicates S. HANSEN and O. JOHNSEN Silver-ion-exchanged Narssarssuk catapleite S. HANSEN, X-ray microanalysis of Na-P zeolites A. LANDA-CANOVAS, U. HAKANSSON and L. FALTH V. HANSEN and J. GJONNES Intermediate structures and _ crystalline approximants in the I>« transformation of Al-(Mn,Fe)-Si alloys V. HANSEN, H. SEIM, Electron microscopy study of some Ni-S H. FJELLVAG and A. OLSEN phases J. HJELEN and Electron channeling contrast (ECC) imaging B.-I. TOLLESHAUG using specimen current S. HOVMOLLER Crystallographic image processing J. S. JAHREN Authigenic clay mineral relations in sand- stones from offshore Norway. The case of illite and chlorite O. JOHNSEN and P. L. HANSEN A TEM study of natural perovskite from Gardiner Complex vi E. JOHNSON, Q. LI, Crystallization in Al-Fe-Y metallic glasses A. JOHANSEN and L. SARHOLT-KRISTENSEN G. KarRLsson, A. CARLSSON Computerized imaging aid for sectioned and J.-O. Bovin RuBisCO crystals N. C. K. Lassen Automatic crystal orientation determination from EBSPs C. K. OLsson, Characterizing the microstructure of alumina L. H. CHRISTENSEN and using image processing L. GRAHL-MADSEN M. H. Loretto State of the art in analytical electron microscopy D. W. McComs Interpretation of electron energy-loss spectra of minerals K. E. MOGENSEN and Preparation of TEM specimens from a har- K. GLEJBOL dened tool steel K. E. MOGENSEN and TEM investigation of an ion implanted steel K. GLEJBOL J. J. L.M ULDERS and A database system for SEM images R. J. ENDERT J. J. L. MULDERS and The influence of parameter settings on speed R. J. ENDERT and accuracy of automatic particle analysis M. PosFaAl and M. SUNDBERG HREM investigations of two minerals, Cu,(As,Sb)S, L. REIMER Energy filtered electron diffraction E. O. RISTOLAINEN and Characterization of the interface and inter- K. A. PiscHow mediate layers of TiN/Ti/substrate systems J. M. RODENBURG A new look at the resolution limit B. SCHMIDT, K. HJEMSTED, Size distributions of In and Pb inclusions in Al E. JOHNSON, K. K. BOURDELLE, A. JOHANSEN, H. H. ANDERSEN and L. SARHOLT-KRISTENSEN S. A. SETHI and A. R. THOLEN Amplitude contrast from small particles J. SkoGsMo, M. HALVARSSON Phase transformation of CVD x-Al,O, and S. VUORINEN B. STENBOM, P. LOOF and Sintering of Pt/Al,O, catalyst inO,,CO, NO H. NorRDEN and CO+ NO atmospheres O. SusEGG, E. HELLUM, An electron microscopy study of dispersoids A. OLSEN and M. LUTON in cryomilled ODS-materials Vii C. TREHOLT TEM cross-section of high-T, superconducting thin films P. VASE Lithographic methods for production of high-T, superconducting thin films devices P. WAHLBERG, Scanning electron microscopy and modern C. VESTERGAARD and telecommunication L. H. CHRISTENSEN L. R. WALLENBERG, Identification of semi-coherent Y,O, inclu- T. I. SELINDER, Z. HAN and sions in YBa,Cu,O, superconducting thin U. HELMERSSON films by HREM G. WATERLOO, R. HoieER and A HRTEM study of twins in CdTe and HgTe K. GJ@NNES Diary Announcement Number 3 DIGITAL OPTICAL MICROSCOPY: CONTRIBUTED REVIEW ARTICLE P. GUALTIERI 239 Molecular biology in living cells by means of digital optical microscopy FIELD EMISSION MICROSCOPY K.-J. Kim, M. R. Dickson, 259 Applications of field emission scanning elec- V. CHEN and A. G. FANE tron microscopy to polymer membrane research BIOLOGICAL APPLICATIONS K. M. KyLe and G. HARAUZ 273 Structures of ribosomal subunits from Sac- charomyces cerevisiae J. R. Harris, Z. CEJKA, 287 Two-dimensional crystallization, transmis- A. WEGENER-STRAKE, sion electron microscopy and image process- W. GEBAUER and J. MARKL ing of keyhole limpet haemocyanin (KLH) PHYSICAL APPLICATIONS H. W. SIMMoNs, Cathodoluminescence of defects in optical C. E. NOCKOLDs, fibres and optical fibre preforms G. R. ATKINS, S. B. POOLE and M. G. ScEATs SCANNING ELECTRON MICROSCOPY D. Jones, W. J. MCHaARDY, 315 Scanning electron microscopy of calcium M. J. WILSON and oxalate on mantle hyphae of hybrid larch D. VAUGHAN roots from a farm forestry experimental site K. KaAnayA, N. ICHISE, 319 Observations on charging effects of non- K. ADACHI, K. YONEHARA and conductive and uncoated materials by ion Y. MURANAKA beam pre-bombardment in scanning electron microscopy Short communication S. Dey and B. DKHAR An unusual type of corneal nipple in the earwig, Forficula sp., with a possible anti- reflection role ABSTRACTS FROM THE MEETING OF THE BIOLOGICAL X-RAY MICROANALYSIS GROUP J. ABRAHAM, R. D. BUTLER 343 X-Ray microanalytical studies on ciliate and D. C. SIGEE protozoa in activated sludge C. BERTONI-FREDDARI, 345 Neurobiology of the aging hippocampus: P. FATTORETTI, T. CASOLI, intranuclear ionic content of CAl pyramidal M. GAMBINI, W. MEIER-RUGE cells and J. ULRICH I. D. Bowen, K. MULLARKEY Backscattered electron imaging, X-ray micro- and N. WorRILL analysis and X-ray microscopy in the study of cell death . CAMPOS, Electron probe X-ray microanalysis of Ca and A. LOPpEZ-ESCAMEZ, K distributions in the otolithic membrane . J. CANIZARES and . V. CRESPO bY P . CASSELLA and A microanalytical study of bone mineral in S. YOUSUF ALI osteogenesis imperfecta T. J. COLLARD, T. A. MARTIN, Effect of lead on growth of Hypholoma C. WINTERS and L. BODDY fasciculare and Phanerochaete velutina P. ComPERE, J. A. MORGAN, X-Ray microanalytical and cytochemical C. WINTERS and G. GOFFINET study of the mineralization process in the shore crab cuticle L. A. CUMMINGS and The localization of acid phosphatase as a I. D. BOWEN prelude to cell death in the blow-fly Calliphora erythrocephala E. EL-BEsTAwy, X-Ray microanalytical studies on phyto- E. BELLINGER, M. HASSAN, plankton and suspended matter in a polluted S. HELM! and D. C. SIGEE freshwater environment: Lake Maryut, Alex- andria, Egypt M. A. HAJIBAGHERI X-Ray microanalysis of ion distribution in Dunaliella parva, following cryofixed, molecular distillation dried and embedded specimens . HATTON and X-Ray microanalysis of bone and implanted bone substitutes . KREFTING, K. FRENTZEL, Quantitative electrorprobe X-ray micro- TEBAREK and analysis on the calcium and phosphorus . HOHLING distribution and calcium transport in epiphy- seal growth plate J. A. LOPEZ-ESCAMEZ, Dependence between saccule and utricle in Ca P. V. Crespo, F. J. CANIZARES and K concentrations determined by electron and A. CAMPOS probe X-ray microanalysis Z. MALIK, T. BABUSHKIN, Cell-death induced by discrete processes: its H. LapDAn, H. WEIs, reflection in cellular ion content revealed by Y. NITZAN, J. SHANANI and X-ray microanalysis D. ZIPor! A. J. MORGAN and Zinc distribution in hamster skin topically C. WINTERS dosed with a zinc-erythromycin complex and zinc acetate: EPXMA in the SEM of cryostat sections A. J. MORGAN, C. WINTERS Drift corrected quantitative elemental map- and N. WILKINSON ping in freeze-dried thin cryosections: metal compartmentation within earthworm chlor- agocytes S. M. Moraan, I. D. BOWEN EPMXA of bulk elemental changes in the and A. J. MORGAN salivary gland of the blowfly (Calliphora vomitoria) during metamorphosis J. A. Nott, K. P. RYAN and X-Ray microanalysis of cadmium in the R. PELc marine snail Littorina littorea (L.) D. T. PATTON and The application of X-ray microanalysis to the G. C. CARNEY tissues of limpets with high metal content, preliminary results K. P. RYAN and Metals in flounder melanomacrophages and A. L. PULSFORD sediments C. SCOTCHFORD and Calcium phosphate microcrystal deposition S. YOUSUF ALI in articular cartilage: characterisation by XRMA S. K. SINGHRAO, J. W. NEAL, X-Ray microanalysis: comparison of mineral G. CoLe and G. R. NEWMAN deposits in Alzheimer’s disease and control tissue using TEM 8: AS MITH, P. WYETH and SEM-EDXA characterisation of metal J. F. V. VINCENT impregnated cuticle xX B. J. THOMAS and X-Ray microanalysis of ion distribution F. R. MINCHIN across the cortex of soybean nodules N. THomas and I. aP GWYNN The quantitation of gold-labelled macromole- cules on cell surfaces by X-ray microanalysis J. Woop The benefits of real time spectrum processing in quantitative X-ray microanalysis J. E. YOUNG and C. WINTERS Localised distribution of copper within mature fern gametophytes Book Reviews Diary New Patents Number 4 THREE-DIMENSIONAL IMAGING 401 Introduction F. BELTRAME, P. RAMOINO, 403 Three-dimensional reconstruction of Para- M. Fato, M. U. DELMONTE mecium primaurelia oral apparatus through CoRRADO, G. MARCENARO confocal laser scanning optical microscopy and T. CrIPPA FRANCESCHI K. CARLSSON, K. MOSSBERG, Use of UV excitation in confocal laser scan- P. J. HELM and J. PHILIP ning fluorescence microscopy T. WILSON and F. REINHOLZ Confocal microscopy via reciprocal optical fibre image detection S. R. GOLDSTEIN, T. HUBIN An improved no-moving-parts video-rate and T. G. SMITH Jr. confocal microscope B. RoysAM, Unsupervised noise removal algorithms for A. K. BHATTACHARJYA, three-dimensional confocal fluorescence C. Srinivas, D. H. SZAROWSKI microscopy and J. N. TURNER N. As-unp and A. LILJEBORG A method to extract homogeneous regions in 3-D confocal microscopy enabling compensa- tion for depth dependent light attenuation xi S. S. S. Poon, R. K. WarRD Feature extraction from three-dimensional and B. PALcic images in quantitative microscopy D. F. ABBOTT and Limitations on cell volume determination K. A. NUGENT using three-dimensional optical microscopy . PREZA, J. M. OLLINGER, Point-spread sensitivity analysis for computa- . G. MCNALLY and tional optical-sectioning microscopy . J. THOMAS JR. . CAPINERI, G. CASTELLINI, Computer simulated benchmarks of synthetic . MasotTi and S. ROCCHI aperture techniques for vascular ultrasono- graphy Diary Index