Microbiology and Biochemistry of Strict Anaerobes Involved in Interspecies Hydrogen Transfer

497 Pages·1990·28.45 MB·English

by  Richard Sparling

Micro biology and Biochemistry of
Strict Anaerobes Involved in
Interspecies Hydrogen Transfer
FEDERATION OF EUROPEAN MICROBIOLOGICAL SOCIETIES
SYMPOSIUM SERIES
Recent FEMS Symposium volumes published by Plenum Press
1990 • MOLECULAR BIOLOGY OF MEMBRANE-BOUND COMPLEXES IN
PHOTOTROPHIC BACTERIA
Edited by Gerhart Drews and Edwin A. Dawes
(FEMS Symposium No. 53)
1990 • MICROBIOLOGY AND BIOCHEMISTRY OF STRICT ANAEROBES
INVOLVED IN INTERSPECIES HYDROGEN TRANSFER
Edited by Jean-Pierre Belaich, Mireille Bruschi, andJean-Jouis Garcia
(FEMS Symposium No. 54)
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tion please contact the publisher.
Microbiology and Biochemistry of
Strict Anaerobes Involved in
Interspecies Hydrogen Transfer
Edited by
Jean-Pie'lie Belaich
and Mirezlle Bruschi
Laboratoire de Chimie Bacterienne I CNRS
Marseille, France
and
Jean-Louis Garcia
ORSTOM/Universite de Provence
Marseille, France
PLENUM PRESS • NEW YORK AND LONDON
Library of Congress Cataloging-in-Publication Data
Microbiology and biochemistry of strict anaerobes involved in
interspecies hydrogen transfer I edited by Jean-Pierre Belaich and
Mireille Bruschi and Jean-Louis Garcia.
Q. em. -- (FEMS symposium; no. 54)
Proceedings of a symposium held under the auspices of the
Federation of European Microbiological Societies, 9112-14189,
Marseille, France.
Includes bibliographical references.
ISBN-13: 978-1-4612-7892-4 e-ISBN-13: 978-1-4613-0613-9
DOl: 10.1007/978-1-4613-0613-9
1. Anaerobic bacteria--Congresses. 2. Dehydrogenases--Congresses.
3. Biomass energy--Congresses. I. Belaich, Jean-Pierre.
II. Bruschi, Mireille. III. GarCia, Jean-Louis. IV. Federation of
European Microbiological Societies. V. Series.
QR89.5.M53 1990
589.9'0128--dc20 90-7023
CIP
Proceedings of a symposium held under the auspices of the
Federation of European Microbiological Societies,
September 12-14, 1989, in Marseille, France
© 1990 Plenum Press, New York
Softcover reprint of the hardcover 1st edition 1990
A Division of Plenum Publishing Corporation
233 Spring Street, New York, N.Y. 10013
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted
in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording, or otherwise, without written permission from the Publisher
PREFACE
The belief that energy might be a limiting factor for the development
of humanity led twenty years ago to a great interest being'taken in
research on anaerobic digestion. The first international symposium held
in Cardiff in 1979 was followed by the meetings in Travenmund (1981),
Boston (1983), Guangzhou (1985) and Bologna (1988). By now anaerobic
digestion has come to be recognized as an appropriate technology for waste
treatment. More recently, the increase in the carbon dioxide content of
the atmosphere and (in developed countries, especially_ in the EEC) the·
fact that more and more land is becoming available for purposes other than
food production make biomass production economically and/or socially
feasible for industrial purposes. The possibility of using renewable
organic carbon resources in this way is of great potential interest for
developing biological techniques and could considerably increase the use
of anaerobic micro-organisms in cellulose biotransformation and energy
production from crop residues.
This FEMS Symposium is devoted to the interspecies hydrogen transfer
phenomenon involved in the mineralization of organic matter in
anaerobiosis. This process is carried out in Nature by consortia of
anaerobic micro-organisms living syntrophically. Many industrial
applications of these consortia as black boxes for biogas production and
waste treatment have been described. Although these early approaches were
fruitful, it seems likely that a better knowledge at the molecular level
of the more characteristic anaerobic bacteria which constitute these
consortia would greatly increase and improve the utilization of these
organisms.
The purpose of this Symposium was to provide an opportunity for
discussing the recent progress which has been made in the biology,
biochemistry and genetics of the anaerobic microbes which participate in
the metabolism of hydrogen. Special attention was paid to bacterial
hydrogenases, key enzymes of hydrogen metabolism, which are responsible
for hydrogen transfer between the various 'partners in anaerobic consortia.
The Symposium was held in Marseille, France from 12-14 September 1989,
sponsored by the Federation of European Microbiological Societies, Agence
Fran~aise pour la Maftrise de l'Energie, Conseil Regional Provence-Alpes
C6te-d'Azur,~the Municipality of Marseille, and Pharmacia of France. The
Organizing Committee of the Symposium consisted of J-P Belaich, K H
Schleifer, Claudine Elmerich, Marie-Claire Blanchard, Pomme Lamy and V A
Jacq and they express their gratitude to the sponsors who enabled the
meeting to take place.
Jean-Pierre Belaich
v
CONTENTS
PART I -PLENARY LECTURES
Molecular Hydrogen and Energy Conservation in Methanogenic and
Acetogenic Bacteria ........................................................................... 3
R. Sparling and G. Gottschalk
Approaches to Gene Transfer in Methanogenic Bacteria. ....................................... ll
T. Leisinger and L. Meile
The Hydrogenase of Methanococcus voltae
an Approach to the Biochemical and Genetic Analysis of
an Archaebacterial Uptake Hydrogenase ................................................. 25
E. Kothe, S. Halboth, J. Sitzmann and A. Klein
Hydrogenase Genes of Desulfovibrio ............................................................. 37
G. Voordouw
The Hydrogenases of Sulfate -Reducing Bacteria:
Physiological, Biochemical and Catalytic Aspects ...................................... 53
E.c. Hatchikian, V.M. Fernandez and R. Cammack
The F420-Reducing Hydrogenase of Methanospirillum hungatei Strain GPl.. ............. 75
G.D. Sprott
Ecological Impact of Syntrophic Alcohol and Fatty Acid Oxidation ........................... 87
A.J.M. Starns and A.J.B. Zehnder
Methanogenesis from Propionate in Sludge and Enrichment Systems ........................ 99
R.A. Mah,'L.Y. Xun, D.R. Boone, B. Ahring, P.H. Smith,
and A. Wilkie
vii
PART2-CONFERENCES
2.1. - MICROBIOLOGY
Substitution ofH2-Acceptor Organism with Catalytic Hydrogenation
System in Methanogen Coupled Fermentations ........................................ 115
D.O. Mountfort and H.F. Kaspar
Hydrogen Transfer in Mixed Cultures of Anaerobic Bacteria and Fungi with
Methanobrevibacter smithii .............................................................. 121
C.S. Stewart, A.J. Richardson, R.M. Douglas and C.J. Rumney
Oxidative Propionate Formation by Anaerobic Bacteria. ..................................... 133
A.T.P. Slcrabanja and A.J.M. Starns
Anaerobic Degradation of Furfural by Defined Mixed Cultures .............................. 141
S.M. Schoberth, U. Ney and H. Sahm
Energetics and Kinetics of Two Complementary Hydrogen Sink Reactions in
a Defined 3-Chlorobenzoate Degrading Methanogenic Consortium ................. 151
J. Dolfing
Role of Hydrogen in the Growth of Mutualistic Methanogenic Cocultures ................. 161
J. Benstead, D;B. Archer and D. Lloyd
Thermodynamical and Microbiological Evidence of Trophic Microniches for
Propionate Degradation in a Methanogenic Sludge-Bed Reactor. ................... 173
S.R. Guiot, F.A. MacLeod and A. Pauss
Dissimilation of Ethanol and Related Compounds by Desulfovibrio Strains .............. 185
T.A. Hansen and D.R. Kremer
Factors Affecting Hydrogen Uptake by Bacteria Growing in the Human
Large Intestine ............................................................................. 191
G.R. Gibson, J.H. Cummings and G.T. Macfarlane
Isolation and Characterization of an Anaerobic Bacterium Degrading
4-Chlorobutyrate ........................................................................... 203
K. Matsuda, K. Nakamura, Y. Kamagata and E. Mikarni
Carbon and Energy Flow During Acetogenic Metabolism ofUnicarbon
and Multicarbon Substrates ............................................................... 213
N.D. Lindley, E. Gros, P. Le Bloas, M. Cocaign and P. Loubiere
Enrichment of a Mesophilic, Syntrophic Bacterial Consortium Converting Acetate
to Methane at High Ammonium Concentrations. ..................................... 225
A. Blomgren, A. Hansen and B. H. Svensson
2.2. - BIOCHEMISTRY
Electron Carrier Proteins in Desulfovibrio vulgaris Miyazaki... ............................. 237
T. Yagi and M. Ogata
Interaction Studies Between Redox Proteins, Cytochrome c3, Ferredoxin and
Hydrogenase from Sulfate Reducing Bacteria. ........................................ 249
A. DoHa, F. Guedesquin, M. Bruschi and R. Haser
viii
Biochemistry of the Methylcoenzyme M Methylreductase System. ......................... 259
P.E. Rouviere, C.H. Kuhner and R.S. Wolfe
Energetics of the Growth of a New Syntrophic Benzoate Degrading Bacterium. .......... 269
J.P. Belaich, P. Heitz, M. Rousset and J.L. Garcia
Syntrophic Propionate Oxidation ................................................................. 281
F.P. Houwen, 1. Plokker, C. Dijkema and A.J.M. Starns
Structural and Functional Properties of the Chromosomal Protein MC1 Isolated
from Various Strains of Methanosarcinaceae ........................................... 291
B. Laine, F. Chartier, M. Imbert and P. Sautiere
2.3. - GENETICS
An Archaebacterial In Vitro Transcription System. ............................................ 305
M. Thomm, G. Frey, W. Hausner and B. Brlidigam
A Survey of Recent Advances in Genetic Engineering in Bacteroides ...................... 313
M. Bechet, P. Pheulpin, 1.C. Joncquiert, Y. Tierny,
and J.B. Guillaume
The Membrane-Bound Hydrogenase of the Photosynthetic Bacterium
Rhodobacter capsulatus ................................................................... 329
P. M . Vignais, A. Colbeau, B. Cauvin and P. Richaud
Hydrogenase Mutants of Escherichia coli Defective in Nickel Uptake ...................... 337
M.A. Mandrand, L.F. Wu and D. Boxer
PART 3 -POSTER SESSION
3.1. - MICROBIOLOGY
Thermophilic Anaerobic Oxidation of Butyrate in Axenic Culture .......................... 351
B. Ahring, P. Westermann and R.A. Mah
Eubacterium acidaminophilum , an Organism Able to Interact
in Interspecies H-Transfer Reactions or to Transfer Electrons
to Different Terminal Reductase Systems ............................................... 355
J.R. Andreesen, K. Hormann, K. Granderath, M. Meyer,
and D. Dietrichs
Immunological Properties of Desulfobacter ..................................................... 359
1. Beeder, T. Lien and T. Torsvik
Fermentation Properties of Four Strictly Anaerobic Rumen Fungal Species:
H 2-Producing Microorganisms .......................................................... 361
A. Bernalier, G. Fonty and Ph. Gouet
Growth of Bacteroides xylanolyticus in the Presence and Absence
of a Methanogen ........................................................................... 365
S. Biesterveld and A.J.M. Starns
Hydrogen Metabolism by Termite Gut Microbes ............................................... 369
A. Brauman, M.D. Kane, M. Labat and J.A. Breznak
ix
Hydrogen and Methanogenesis in Rumen Liquor and in Rumen Ciliate /
Methanogen Cocultures ................................................................... 373
J. Ellis, K. Hillman, A.G. Williams and D. Lloyd
Hydrogen Production by Rumen Ciliate Protozoa ............................................. 377
J. Ellis, K. Hillman, A.G. Williams and D. Lloyd
Methanogenic Bacteria and their Activity in a Subsurface Reservoir
of Town Gas ............................................................................... 381
M. Greksfr, P. Smigan, J. Kozlinkova, F. Buzek,
V. Onderka and I. Wolf
Study of the Transition of Activated Sludges to an Adapted Anaerobic
Inoculum for Anaerobic Digestion ....................................................... 385
J.P. Guyot, C. Fajardo, A. Noyola and C. Barrena
Effects of Elevated Hydrogen Partial Pressures on
Anaerobic Treatment of Carbohydrate .................................................. 387
S.R. Harper and F.G. Pohland
Hydrogen Production in Anaerobic Biofilms ................................................... 391
E. Henry, T. Ford and R. Mitchell
Effects of Various Headspace Gases on the Production of Volatile Fatty Acids by
Rumen Ciliate Protozoa ................................................................... 395
K. Hillman, A.G. Williams and D. Lloyd
Mass Transfer of Hydrogen in a Culture of Methanobacteriwn thermoautotrophicwn
Strain Hveragerdi .......................................................................... 399
G. Jud, K. Schneider and R. Bachofen
Physiology and Metabolic Features of a Novel Methanogenic Isolate ...................... .403
S. Krishnan and K. Lalitha
Thermophilic Anaerobic Oxidation of EthanoL ............................................... .407
S. Larsen and B.K. Ahring
Efficiency of Bacterial Protein Synthesis and Methanogenesis during
Anaerobic Degradation ................................................................... 411
R.1. Mackie and M.P. Bryant
Trace Methane in some Proteolytic Nonglucidolytic Clostridia:
the Role of Some S-Methyl and N-Methyl Compounds ............................. .415
P. Niel, G. Leluan, H. Virelizier and A. Rirnbault
Inhibition Kinetics by H2. Acetate and Propionate in Methanogenesis
from Propionate in a Mixed Culture ................................................... .419
N. Nishio, S. Fukuzaki and S. Nagai
Hydrogep Content in Biogas as a State Indicator of Methanogenesis
from Wastes ............................................................................... 423
A.N. Nozhevnikova, LV. Bodnar, A.1. Slobodkin and T.G. Sokolova
Interspecies Transport of Hydrogen in Thermophilic Anaerobic
Cellulose Decomposition ................................................................ .427
A.N. Nozhevnikova and M.V. Simankova
x
The Participation of Lithotrophic Homoacetogenic Bacteria and M ethanothrix in
Thermophilic Anaerobic Ethanol Degradation with Methane Formation ........... .431
AN. Nozhevnikova and A.I. Slobodkin
Characterization of a Sulfate Reducing Bacterium Isolated from
a Hypersaline African Lake .............................................................. .435
B. Ollivier, C.E. Hatchikian, G. Prensier, J. Guezenec,
and J.L. Garcia
Isolation and Characterization of an Ethanol-Degrading Anaerobe
from Methanogenic Granular Sludge ................................................... .439
C.M. Plugge, J.T.e. Grotenhuis and AJ.M. Starns
The Role of Fe(III) Reduction in Anaerobic Processes ....................................... .443
J. Potekhina
Glycerol Degradation by Desulfovibrio sp. in Pure Culture and
in Coculture with Methanospirillum hungatei ........................................ .447
A.I. Qatibi, J.L. Cayol and J.L. Garcia
1,2-and 1,3-Propanediol Degradation by Desulfovibrio alcoholovorans sp. nov.,
in Pure Culture or through H2 Interspecies Transfer. ... : ...- ......................... .451
AI. Qatibi and J.L. Garcia
Long Chain Fatty Acid Degradation by a Mesophilic Syntrophic Coculture
Isolated from Margin ..................................................................... 455
M. Ragot, B. Ollivier and J.L. Garcia
Effect of Sulfide and Reactor Operational Parameters on Sulfate Reducing Bacteria ..... .459
M.A.M. Reis, P.e. Lemos, J. Almeida and M.T.J. Carrondo
Hydrogen Transfer between Neocallimastixfrontalis and
Selenomonas ruminantium Grown in Mixed Culture ................................ .463
AJ. Richardson and C.S. Stewart
Phylogenetic Analysis of Methanogenic Bacteria. ............................................. .467
P. Rouviere, L.C. Mandelco and e.R. Woese
An Acetate Decomposing Sulphidogenic Syntrophic Association .......................... .469
E. Rozanova, A. Galushko and T. Nazina
Anaerobic Digestion of Proteins, Peptides and Amino Acids ................................ .471
J.P. Schwitzguebel and P. Peringer
Isolation of Syntrophic Bacteria on Metabolic Intermediates ................................ .473
AJ.M. Starns and C.M. Plugge
Isolation of Propionate-Using, Sulfate-Reducing Bacterium ................................ .477
M. Tasaki, Y. Kamagata, K. Nakamura and E. Mikami
Characterization of a Sulfate-Reducing Bacterium Isolated from
the Gut of a Tropical Soil Termite ...................................................... .481
S.A. Traore, G. Fauque, V.A Jacq and J.P. Belaich
Relationship between Methanogenesis and Sulfate Reduction in Anaerobic Digestion
of Municipal Sewage Sludge ............................................................ .485
K. Ueki and A Ueki
xi