MICROALGAL BIOTECHNOLOGY Bioprospecting Microalgae for Functional Metabolites towards Commercial and Sustainable Applications Innovations in Biotechnology MICROALGAL BIOTECHNOLOGY Bioprospecting Microalgae for Functional Metabolites towards Commercial and Sustainable Applications Edited by Jeyabalan Sangeetha, PhD Svetlana Codreanu, PhD Devarajan Thangadurai, PhD First edition published 2023 Apple Academic Press Inc. CRC Press 1265 Goldenrod Circle, NE, 6000 Broken Sound Parkway NW, Palm Bay, FL 32905 USA Suite 300, Boca Raton, FL 33487-2742 USA 760 Laurentian Drive, Unit 19, 4 Park Square, Milton Park, Burlington, ON L7N 0A4, CANADA Abingdon, Oxon, OX14 4RN UK © 2023 by Apple Academic Press, Inc. 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Library and Archives Canada Cataloguing in Publication Title: Microalgal biotechnology : bioprospecting microalgae for functional metabolites towards commercial and sustainable applications / edited by Jeyabalan Sangeetha, PhD, Svetlana Codreanu, PhD, Devarajan Thangadurai, PhD. Names: Sangeetha, Jeyabalan, editor. | Codreanu, Svetlana, editor. | Thangadurai, Devarajan, 1976- Series: Innovations in biotechnology (Series) Description: First edition. | Series statement: Innovations in biotechnology | Includes bibliographical references and index. Identifiers: Canadiana (print) 20220400172 | Canadiana (ebook) 20220400199 | ISBN 9781774912379 (hardcover) | ISBN 9781774912386 (softcover) | ISBN 9781003332251 (ebook) Subjects: LCSH: Microalgae—Biotechnology. | LCSH: Microalgae—Industrial applications. Classification: LCC TP248.27.A46 M48 2023 | DDC 660.6—dc23 Library of Congress Cataloging-in-Publication Data Names: Sangeetha, Jeyabalan, editor. | Codreanu, Svetlana, editor. | Thangadurai, Devarajan, 1976- editor. Title: Microalgal biotechnology : bioprospecting microalgae for functional metabolites towards commercial and sustainable applications / edited by Jeyabalan Sangeetha, PhD, Svetlana Codreanu, PhD, Devarajan Thangadurai, PhD. Description: First edition. | Palm Bay, FL : Apple Academic Press ; Boca Raton, FL, USA : CRC Press, 2023. | Series: Innovations in biotechnology | Includes bibliographical references and index. | Summary: “Microalgae are a valuable resource of carbon materials that may be used in biofuels, pharmaceuticals, cosmetics, and health supplements. Still, there are tremendous challenges in the microalgae production process, such as mass cultivation, strain improvement, biomass disruption, and reprocessing of nutrients and water that have been the encumbering microalgal industry in several ways. Microalgal biotechnology has the capability to introduce remarkable breakthroughs and innovations. This volume brings together current advancements in the field of microalgal biotechnology to provide understanding of the fundamentals and progress of the industry-oriented technologies. The volume covers biofuel production and its technical challenges; current trends of microalgae in agriculture, aquaculture and food biotechnology; market space; biosafety and other regulatory issues; and sustainability of microalgal products. The volume first provides insight into desmids and other conjugating algae along with prospective trends of diatoms. It then goes on to explore the commercial applications of microalgae that take advantage of their bioactive compounds, natural colorants, and functional metabolites. The role of microalgae in agriculture, aquaculture, and the food sector are discussed, and tools from genome manipulation to bioprocess engineering are studied. Several chapters focus on future prospects of biofuel and biodiesel production for environmental and economic viability. The key features of the book: Presents the role of microalgae in various industries including food, agriculture, aquaculture, biofuel, and metabolites Shows the historical and prospective use of microalgae elements for economic and ecological benefits Explains the integrated technologies for massive production of microalgae- derived products Includes the various industrial case studies to improve the sustainable production of microalgae products Discusses current developments and confronts in microalgae bioprocessing Microalgal Biotechnology: Bioprospecting Microalgae for Functional Metabolites towards Commercial and Sustainable Applications will be a useful reference for academicians, researchers, industry professionals, postgraduate students, and policymakers who are interested in future prospects of microalgae commercialization”-- Provided by publisher. Identifiers: LCCN 2022037400 (print) | LCCN 2022037401 (ebook) | ISBN 9781774912379 (hbk) | ISBN 9781774912386 (pbk) | ISBN 9781003332251 (ebk) Subjects: LCSH: Microalgae--Biotechnology. Classification: LCC TP248.27.A46 M525 2023 (print) | LCC TP248.27.A46 (ebook) | DDC 660.6--dc23/eng/20220829 LC record available at https://lccn.loc.gov/2022037400 LC ebook record available at https://lccn.loc.gov/2022037401 ISBN: 978-1-77491-237-9 (hbk) ISBN: 978-1-77491-238-6 (pbk) ISBN: 978-1-00333-225-1 (ebk) BOOK SERIES Innovations in Biotechnology Series Editor Devarajan Thangadurai, PhD Professor, Karnatak University, Dharwad, India Books in the Series • Fundamentals of Molecular Mycology Authors: Devarajan Thangadurai, PhD, Jeyabalan Sangeetha, PhD, and Muniswamy David, PhD • Biotechnology of Microorganisms: Diversity, Improvement, and Application of Microbes for Food Processing, Healthcare, Environmental Safety, and Agriculture Editors: Jeyabalan Sangeetha, PhD, Devarajan Thangadurai, PhD, Somboon Tanasupawat, PhD, and Pradnya Pralhad Kanekar, PhD • Phycobiotechnology: Biodiversity and Biotechnology of Algae and Algal Products for Food, Feed, and Fuel Editors: Jeyabalan Sangeetha, PhD, Devarajan Thangadurai, PhD, Sanyasi Elumalai, PhD, and Shivasharana Chandrabanda Thimmappa, PhD • Biogenic Nanomaterials: Structural Properties and Functional Applications Editors: Devarajan Thangadurai, PhD, Saher Islam, PhD, Jeyabalan Sangeetha, PhD, and Natália Martins, PhD • Seaweed Biotechnology: Biodiversity and Biotechnology of Seaweeds and Their Applications Editors: Jeyabalan Sangeetha, PhD, and Devarajan Thangadurai, PhD • Microalgal Biotechnology: Bioprospecting Microalgae for Functional Metabolites towards Commercial and Sustainable Applications Editors: Jeyabalan Sangeetha, PhD, Svetlana Codreanu, PhD, and Devarajan Thangadurai, PhD • Algal Metabolites: Biotechnological, Commercial, and Industrial Applications Editors: Jeyabalan Sangeetha, PhD, Devarajan Thangadurai, PhD, Saher Islam, PhD, and Ravichandra Hospet, PhD About the Editors Jeyabalan Sangeetha, PhD Assistant Professor, Central University of Kerala, Kasaragod, Kerala, India Jeyabalan Sangeetha, PhD, is an Assistant Professor at Central University of Kerala, Kasaragod, South India. She has edited/co-edited several books in her research areas, which include environmental toxicology, environ- mental microbiology, environmental biotechnology, and environmental nanotechnology. She earned her BSc in Microbiology and PhD in Envi- ronmental Science from Bharathidasan University, Tiruchirappalli, Tamil Nadu, India. She holds an MSc in Environmental Science from Bharathiar University, Coimbatore, Tamil Nadu, India. She is the recipient of a Tamil Nadu Government Scholarship and a Rajiv Gandhi National Fellowship of the University Grants Commission (UGC), Government of India, for her doctoral studies. She served as Dr. D.S. Kothari Postdoctoral Fellow and UGC Postdoctoral Fellow at Karnatak University, Dharwad, South India, during 2012–2016 with funding from the University Grants Commission, Government of India, New Delhi. Svetlana Codreanu, PhD Associate Professor, Institute of Microbiology and Biotechnology, Chisinau, Republic of Moldova Svetlana Codreanu, PhD, is an Associate Professor at the Institute of Microbiology and Biotechnology in Chisinau, Republic of Moldova, is co-founder of Moldovan Society for Microbiology and FEMS Delegate. She studied Biology and obtained her PhD in Microbiology from Moldova State University. Her current scientific research focuses on Applied Phycology and Biotechnology. She is regularly involved as an evaluator of scientific projects and research infrastructures, reviewer of scientific jour- nals, and abstract selections in conferences and PhD defenses. She is the author and co-author of over 100 scientific publications and 17 patents. She received numerous awards, including the WIPO Award and Gold Medal. viii About the Editors Devarajan Thangadurai, PhD Professor, Karnatak University, Dharwad, India Devarajan Thangadurai, PhD, is a Professor of Botany at Karnatak Univer- sity in South India. He has authored/edited over 30 books with national and international publishers and has visited 24 countries in Asia, Europe, Africa, and the Middle East for academic visits, scientific meetings, and international collaborations. He received his PhD in Botany from Sri Krish- nadevaraya University in South India as a CSIR Senior Research Fellow with funding from the Ministry of Science and Technology, Government of India. He served as a Postdoctoral Fellow at the University of Madeira, Portugal; University of Delhi, India; and ICAR National Research Center for Banana, India. He is the recipient of a Best Young Scientist Award with a Gold Medal from Acharya Nagarjuna University, India, and the VGST-SMYSR Young Scientist Award of the Government of Karnataka, Republic of India. Contents Contributors ......................................................................................................... xi Abbreviations .....................................................................................................xiii Preface .............................................................................................................. xvii 1. Applied Studies on Desmids and Other Conjugating Algae (Zygnematophyceae, Streptophyta) .................................................. 1 Marija Stamenković and Pavle Pavlović 2. Diatoms: Commercial Applications and Prospective Trends ................ 45 Manjita Mishra and Shanthy Sundaram 3. Microalgae as a Potential Source of Bioactive Compounds and Functional Ingredients ................................................ 87 Md. Akhlaqur Rahman, Rupali Kaur, and Shanthy Sundaram 4. Microalgae: A Valuable Source of Natural Colorants for Commercial Applications ....................................................................... 121 Chidambaram Kulandaisamy Venil, Matheswaran Yaamini, and Laurent Dufossé 5. Functional Metabolites from Microalgae for Multiple Commercial Streams ............................................................................... 149 Amritpreet Kaur, Suchitra Gaur, and Alok Adholeya 6. Role of Microalgae in Agriculture ......................................................... 183 Rayanee Chaudhuri and Paramasivan Balasubramanian 7. Potential Application of Microalgae in Aquaculture ........................... 205 Pankaj Kumar Singh and Archana Tiwari 8. Microalgal Food Biotechnology: Prospects and Applications ............. 225 Aneela Nawaz, Usman Ali Chaudhry, Malik Badshah, and Samiullah Khan 9. Biofuel Production from Microalgae: Current Trends and Future Perspectives ................................................................................. 251 Pavithra Suresh, Aavany Balasubramanian, and Jyothish Jayakumar