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Methods and apparatus for spinning spider silk protein PDF

41 Pages·2013·2.99 MB·English
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Preview Methods and apparatus for spinning spider silk protein

US 20040102614A1 United States (19) (12) Patent Application Publication (10) Pub. No.: US 2004/0102614 A1 May 27, 2004 Islam et al. (43) Pub. Date: (54) METHODS AND APPARATUS FOR (63) Continuation-in-part of application No. 10/341,096, SPINNING SPIDER SILK PROTEIN ?led on Jan. 13, 2003, noW abandoned. (76) Inventors: Sha?ul Islam, HaWkesbury (CA); (60) Provisional application No. 60/347,510, ?led on Jan. Costas Karatzas, Beacons?eld (CA); 11, 2002. Provisional application No. 60/408,530, Andrew Rodenhiser, Montreal (CA); ?led on Sep. 4, 2002. Ali Alwattari, Point Claire (CA); Yue Huang, Vaudreuil-Dorian (CA); Carl Publication Classi?cation Turcotte, Montreal (CA) (51) Int. Cl.7 ......................... .. D01D 5/30; C07K 14/435 Correspondence Address: (52) US. Cl. ..................................... .. 530/353; 264/172.11 JONES DAY (57) ABSTRACT 222 EAST 41ST STREET The invention features methods and apparatuses for spinning NEW YORK, NY 10017 (US) silk protein ?bers (bio?laments) from recombinant bio?la (21) Appl. No.: 10/406,832 ment proteins. The methods are particularly useful for spinning ?bers of spider silk or silkworm silk proteins from (22) Filed: Apr. 3, 2003 recombinant mammalian cells and may be used to spin such ?bers for use in the manufacture of industrial and commer Related US. Application Data cial products. Patent Application Publication May 27, 2004 Sheet 1 0f 9 US 2004/0102614 Al w(MICROPROCB"CCOO SNN'STORSLOELO R "O. l-i .1 F -L_J g ° u. Patent Application Publication May 27, 2004 Sheet 2 0f 9 US 2004/0102614 A1 2.625 E .520 114-28 3 0 4 s FIG. 2 Patent Application Publication May 27, 2004 Sheet 3 0f 9 US 2004/0102614 A1 FIG. 3 Patent Application Publication May 27, 2004 Sheet 4 0f 9 US 2004/0102614 A1 FIG. 4 Patent Application Publication May 27, 2004 Sheet 5 0f 9 US 2004/0102614 A1 - {Ff-fig. * ~ ~ " I- III-AI“ "iv FIG. 5 Patent Application Publication May 27, 2004 Sheet 8 0f 9 US 2004/0102614 A1 En Eu» £3.9. 8.wo.ommw.oo3w5,mwog ?2og?oo,w?oo2w3G3 .2“ ?oamnn.owmn [email protected]?wao0o»momwog3awg mwua3oom?o ou 2wwmuwuu awnuowno?mmmmmu 6oou2muao5guao2mwummwwwmw .u.m .muuowru4m w.4oo6? u2aou5. mw4ou u 6wE. ... .. ...7. .. 2.0 0 [email protected] 033332503 gEulau.o. uum muo.oo aguoo omum2mauu on?nu.ou».wm uo . o.‘um4uo5ouw2 um4uwmuum 2.8.5 0x3.360 2 8i43 u463m.3mm2.. 3i“? m uow.»wwuooo gmuo'o wauoou2wwmuhbo canwuo»wumw vo.aau ém?.um?u znumu? g- unmu.omvu m m u2oomu5oomw5uaun3wo a 0mm?C.7. é rawl s . 52 gmoewHmgmgcmmmamd,m »s Patent Application Publication May 27, 2004 Sheet 9 0f 9 US 2004/0102614 A1 sample Buffer Tank Tank r Clari?cation Pump ‘pm I TFF C ' 1 Cartridge 750kDCuin? g9 @ Relenlate P ‘ k + Q , ' ' ‘ ermea Rm tate ' . Feed n Cmomb'ation Tank Whey TFF Cartridge ‘?nk 30mm Pump HM —> A Exchanger Pump 8 Wm Consent-ah FIG. 9

Description:
ment, bio?lament ?bers are Wet spun from an aqueous dope solution of or aeroponics are useful for groWing the transgenic plants of interest
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