Membrane Biophysics: Planar Lipid Bilayers and Spherical Liposomes

651 Pages·2000·6.876 MB·English

by  H. Ti Tien and Angelica Ottova-Leitmannova (Eds.)

MEMBRANE BIOPHYSICS
AS VIEWED FROM EXPERIMENTAL
BILAYER LIPID MEMBRANES
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Membrane Science and Technology Series, 5
MEMBRANE BIOPHYSICS
AS VIEWED FROM EXPERIMENTAL
BILAYER LIPID MEMBRANES
(Planar Lipid Bilayers and Spherical Liposomes)
H. Ti Tien
and
Angelica Ottova-Leitmannova
Department of Physiology, Michigan State University
East Lansing, M148824, USA
2000
ELSEVIER
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First edition 2000
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To see the world in a grain of sand,
eternity in an hour,
and hold the world in the palm of your hand,
heaven in a wild flower. -William Blake
Beholding life in a soap bubble,
Self-assembly is the key.
To make a BLM in vitro,
living complexity!
Shows the biomembrane's
-- SST
'2-b
Nick, Jesse,
James, Daniel,
Caroline, Mark,
Christopher, Julian,
Patrick, Veronika,
etc.
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PREFACE
This collection of chapters is a textbook intended for undergraduate
and graduate students interested in membrane biophysics. The book, a gen-
eral introductory course is also aimed at students majoring in any fields of
biological and physical sciences, including physiology, chemistry, physics,
mathematics, engineering or applied sciences and technologies, who would
like to be conversant with historical as well as the latest development in
molecular serf-assemblies, in particular, the lipid bilayer structure of the cell
membrane.
The area of membrane biophysics is perused from the origin of the
lipid bilayer concept to membrane transport, electrochemistry, physiology,
bioenergetics, and to membrane photobiology. Emphasis is placed on theo-
retical and experimental model systems, as related to the structure and
function of biomembranes. The connecting thread, among these topics, is
experimental bilayer lipid membrane (mainly planar BLMs, supported
BLMs, and spherical liposomes). The basic principles of thermodynamics,
kinetics, the Nernst-Planck equation, colloid and surface chemistry are ap-
plied to the above-mentioned topics of membrane biophysics. Some practi-
cal applications of self-assembled lipid bilayers, for example, as biosensors
are also presented. This is due to the fact that recent advances in the devel-
opment of supported planar lipid bilayers (s-BLMs) for biosensing applica-
tions. Such s-BLMs have a unique role to play in interfacing conventional
metal and semiconductor devices to the biological world. The biomimetic
nature of such systems coupled with membrane research of the past dec-
ades has come of age, and is poised for biotechnological exploitation.
vii
ACKNOWLEDGEMENT
Mthough the present work is not a sequel to the earlier book on
'Bilayer Lipid Membranes (BLM): Theory and Practice', published in
1974, the authors are grateful to their co-workers and colleagues since that
time. We thank (listed chronologically in order of association) W. Stillwell,
J. M. Mountz, J. D. Mountz, J. Higgins, J. Lopez, F. A. Siddiqi, Q. Y. Liu,
N. B. Joshi, K. O'Boyle, X. R. Xiao, J. Kutnik, P. M. Vassilev, M. P.
Kanazirska, Z. C. B i, X. C. Shen, Z. K. Lojewska, J.-Y. Yu, P. Krysinski,
G. Xu, B. Shi, S. Mureramanzi, C. Bender, K. Jackowska, J. Zon, R.
Bhardwaj, T. Janas, Z. Salamon, J. Kotowski, J.-W. Chen, V. Kochev, K.
T. L. De Silva, C. Bhardwaj, A. Wardak, T. Martynski, M. Zviman, D. L.
Guo, A. Tripathy, W. Liu, W. Ziegler, V. Tvarozek, J. Sabo, T. Hianik,
XianDao Lu, and K. Asaka.
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TABLE OF CONTENTS
Preface
Chapter 1
Membrane Biophysics as Viewed from Experimental
Bilayer Lipid Membranes (Planar BLMs and Liposomes)
Chapter 2
Fundamental Aspects of Biological Membranes 23
Chapter 3
Membrane Biophysics-- Basic Principles 83
Chapter 4
Experimental Models of Biomembranes 149
Chapter 5
Membrane Transport 221
Chapter 6
Membrane Electrochemistry 283
Chapter 7
Membrane Physiology 349
Chapter 8
Membrane Bioenergetics 443
Chapter 9
Membrane Photobiophysics and Photobiology 493
Chapter 10
Applications 577
Index 639
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