DOCUMENT RESUME CE 044 810 ED 272 711 Thompson, Joselyn H. AUTHOR Medical Laboratory Technician (Chemistry and TITLE Urinalysis). (AFSC 92470). Air Univ., Gunter AFS, Ala. Extension Course Inst. INSTITUTION PUB DATE 85 441p.; Supersedes ED 224 891. NOTE Materials (For Learner) Guides - Classroom Use PUB TYPE (051) MF01/PC18 Plus Postage. EDRS PRICE *Allied Health Occupations Education; Behavioral DESCRIPTORS Objectives; Biomedical Equipment; *Chemical Analysis; Correspondence Study; Equipment Utilization; *Laboratory Procedures; *Laboratory Technology; Learning Activities; *Medical Laboratory Assistants; *Medical Technologists; Military Personnel; Military Training; Postsecondary Education; Quality Control Air Force; Military Curriculum Materials; IDENTIFIERS *Urinalysis ABSTRACT This four-volume student text is designed for use by Air Force personnel enrolled in a self-study extension course for medical laboratory technicians. Covered in the individual volumes are medical laboratory administration and clinical chemistry (career opportunities, general laboratory safety and materials, general medical laboratory administration, basic chemistry, solutions, photometry and spectrophotometry, automation, quality control in collection and handling of specimens); laboratory procedures in clinical chemistry (body metabolism; proteins, carbohydrates, and special enzymes; liver and kidney function tests; gastric analysis; chemistry tests; toxicology) and laboratory procedures in urinalysis (general considerations, renal functions, physical characteristics of urine, microscopic examination, and chemical examination). Each volume in the set contains a series of lessons, exercises at the end of each lesson, a bibliography, and answers to the exercises. Volume review exercises are also included. (MN) *********************************************************************** Reproductions supplied by EDRS are the best that can be made from the original document. *********************************************************************** MEDICAL LABORATORY TECHNICIAN (CHEMISTRY AND URINALYSIS) (AFSC 92470) BEST COPY AVAILABLE U.S. DEPARTMENT OF EDUCATION Office of F ducahonai Research and Improvemenr EDUCATIONAL RESOURCES INFORMATION CENTER (ERIC) docfle,l has been reproduced as rece,ved from the Person or 0,garezatfon orej.nating ft o MmOr changes have teen rnade to improve reproducfion quahly stat.,d po,nts of v,ew or opOlons fl thi5 dOc u ment do not necessardy represent oftfc.al OE RI pos.hon or policy Extension Course Institute Air University ECI COURSE MATERIALS SHIPPING LIST EF FECTIVE DATE COURSE TITLE DURSE NUMBER MEDICAL LABCRATORY TECHN IC IAN CI-THATS TRY AND URINALYS IS) (AFSC 92470) Jun 2 40 41 upon receiving your .uurav package. and needed to oornplete this course. Check this list immediately materials are INSTRUCIIONS: 7.14,7 lollowg immediately. Else the EC1 Form 17 for this purpose, and be sure to include any materials are missing or incorrect (nI4mberl don't match ), notify ECI corresponth,nce separately it- address, course and volume number, and VRE form designation (if a VRE is involved). Send all identification number, Your from your answer ghee t. VRE ANSWER INVENTORY CONTROL SHEET DESIGNATION OR TITLE TYPE ITEM NUMBER IDENTIFICATION VOL 1, Introduction to Medical Laboratory 90411 VOI, 01 3 851_0 Administration ari to Clinical Chemistry VR 90411 VOLUME RF:\/ I EW EXERC 4 fn E (VOL 1_ 01 90411 24 24 ) VoL VOL 2, Laboratory Procedures in Clinical 7608 02 90411 5 Chemistry (Part I) VOLUME REVIEW EXERC VOL 2 90411 90411 6 VIRE E 02 23 23 02 ) ( VOL 90411 VOL 3, Laboratory Procedures in Clinical 7608 03 7 Chemistry (Part II) 23 VRE 90411 VOLUME REVIEW EXERCISE (VOL 3) 90411 03 23 03 8 90411 VOL 4, Laboratory Procedures in Urinalysis VOL 7608 04 9 VRE 90411 90411 VOLUME REVIEW EXERCISE (VOL 4) 04 04 23 23 10 DI RECT AN Y QU NOTE : T1(3\IS OR COMM1I\T IS RK LAT TNG `TO ACCURACY OF 'TEXTUAL MATER HUE AUTOVCN 736-4471. DISREGARD ME MSC 90470 SPECIAL 1\10TE: calm av ThE PRINTED M IS OF ME TEX' IS . DC IS FOR AIRMEN CN UPGRADE TRAMING TO AFS C 92470 ONLY. SEP: REVI,RSE SIDE FOR ADDITP)NAL INSTRUCTIONS. ECI FORM 25 PREVIOUS EDITION WILL 1.1E USED. JUN 8 4 Page I. of 3 LIST OF CHANGES OURSE NUMDED CAREER FIELDS, POLICIES, PROCEDURES AND EQUIPMENT CHANGE. ALSO, )041) ERRORS OCCASIONALLY GET INTO PRINT. THE FOLLOWING FILMS UPDATE ANI) FFECTtVE DATE OF CORRECT YOUR COURSE MATERIALS. PLEASE MAKE THE INDICATED CHANGES. IPPS.111 ;PECIAL NUPE: THROJT TriE TEXTS AND VOLUME REV1E4 EXERCISE; FOR THIS COURSI.:, (1LVilE FFR;T 3 DIGUIS OF ALL AFSCS FROM 904 TO 924. CHANGES FOR THE TEXT: VOLUME I 1. Page 67, col 2, line 6 fr bot: Change "octet" to "completion." a. Page 84, Exercise (052)-1.(10): Change "buffered" to "unbuffered." b. Page c. ,ver 011-1(7): Change "f" to "c." Page 160, answer 029-3: result Change "Take a few d. t)ort" to "Correct." . . . Answer 033-7: Change "No" to "Yes" and delete "not" in both places. 161, answer 042-10: Change "oxygen" to "hydrogen." e. Par! 1-3e 163, answer 057-(1): Change "1" to "k." f. Page 165, answer 074-8: g. After "as" add "those." Col 1, line 11 fr bot, answer 075-6: Change "serum" to "the cells." CHANGES FOR THE TEXT: VOLUME 2 2. Page 52, col 2, last line: Change "LDH" to "LAP." a. Page 53, col 1, lines 1 and 3: Change "LDH" to "LAP." b. CHANGE FOR PHE TEXT VOLUME 3 3. Page 56, col 1, line 12: Change Change "facilities" to "fatalities." Col line 12: "423" to "433." ME REVIEW EXERCISE: VOLUME 1 CHANGES PT.% THE ,c1; 4. Page 6, question 34: In the stem of the question, change ",-,ervic to "service; a. . however, this was not expected to cover the manning shortage in the blood bank." Question 10 is no longer scored and need not be answered. b. CHANGES FOR THE VOLUME REVIEW EXERCISE: VOLUME 2 5. Pale 3, question 15, choice a: Change "incerase" to "increase." Choice a. Change the second "decrease" to "increase." b: in the stem of the question, delete ", srum biliruhin is high page 5, querltion 31: b. and." Page 6, question 38: In the stem of the question, change "Frog" to "Gror." c. Page 9, question 72, choice a: Change "phenlphethalein" to "phenolphthalein." d. Change "methly" to "methyl." Choice c: Page 11, question 81: In the stem of the question, change "catalyze" to "catalyzes." e. 4 Continuation Sheet for ECI Form 25 Page 2 of 3 LIST OF CHANGES 1URS NUM ALSO, CAREER FIELDS, POLICIES, PROCEDURES AND EQUIPMENT CHANGE. ,0411 ERRORS OCCASIONALLY GET INTO PRINT. THE FOLLOWING ITEMS UPDATE AND 'FECTIVE DATE OF ITPTILIAT) CORRECT YOUR COURSE MATERIALS. PLEASE MAKE THE INDICATED CHANGES. CHANGES FOR THE VOLUME REVIEW EXERCISE: VOLUME 2 (Continued) Change "exalacetate" to "oxalacetate." Page 13, question 105, choices a and c: f. The following questions are no longer scored and need not be answered: g. 7 and 81. VOLUME 3 ChANGF:'; FOR THE VOLUME REVIEW EXT-RCESE: rage 2, quetion 5, choice a: Change "ureas" to "urea." a. Qurstion Page 3, question 11: In the stri of the question, change "text." to "I-est." b. In the stem of the question, change "insufficiently" to "insufficiency." .4: 7, question 44, choice c: Question 50: Change "nitrose" to "nitroso." In the c. itam of guestion, change "materia" to "material." Page 9, question 64, choice d: Change "dodium hydrosulfide." to "sodium d. Iydrosulfite." Questf.m 68: In the stem of the question, change "gives a positive tPst" 'will cause the iminadiate appearance of a sliight turbidity." The following questions are no longer scored and need not be answered: e. 19 and 65. VOLUME 4 CHANGES FOR THE VOLUME REVIEW EXERCE3E: 7. Page 3, question 15: In the stem of the question, change "pres!-.;ure" to "17.2sence." a. In the stem of the question, after "solution" add a comm. Page 5, question 31: b. Page 6, question 40: In the stem of the question, remove underline from "describing" c. and under line "transudation." Qua;tion 84, Page 10, question 80, choice c: Change "Abermaver" to "Obermayer." d. "orthotolodine" to "orthotolidine." :thoice 6: Change "(115)" to "(638)." Page 11, question 88: P. Question 72 is no longer scored and need not be answered. t. rowlAMMI Page 3 of 3 l'ontinuation Sheet for ECI Form 25 t- Prepared by CMSgt Joselyn H. Thompson (AUTOVON: 736-2809) Reviewed by Elmore C. Hall Edited by Joyce Wagner Anderson SCIENCES, t SCHOOL OF HEA1 T-rt CA -t-C) Atr:F j TEXAS SHEPPARD AIR r EXTENSION COURSE INSTITUTE (AU) GUNTER AIR FORCE STATION, ALABAMA 36118-5643 7 Preface THE AIR FORCE laboratory technician should possess and demonstrate the technical ability to effectively deliver quality laboratory service. Quality laboratory service is essential to the accurate diagnosis and treatment of patients. Laboratory technicians, today and in the future, should use good supervisory and administrative skills for efficient management of the medical laboratory. This volume provides you with a background of basic information so that together with your on-the-job training and experience, you should easily make the transition from the 5-level specialist to the 7-level technician. This CDC 90411 is the first of three career development courses for the 92470 specialty. Chapter 1 of this volume introduces the mission and capabilities of Air Force clinical laboratories and guidelines in career progression. In chapter 2, you will review current principles of laboratory materiel and general laboratory safety. Chapter 3 reviews general laboratory administration, emphasizing laboratory ethics and interpersonal relations, laboratory reports, and accreditation program. Chapters 4 and 5 cover a review of basic chemistry and the nature and concentration of laboratory solutions. Chapter 6 is about basic principles of photometry and spectrophotometry. Chapter 7 reviews principles, techniques, and instruments used in automation. Chapter 8 concludes with further principles and techniques for collection and handling laboratory specimens and a review of quality control in the clinical laboratory. For your convenience in the course and future reference, the Appendixes contain the duties and responsibilities of your specialty, a Periodic Chart, and a summary of general laboratory formulae. Code numbers appearing on figures are for preparing agency identification only. The inclusion of names of any specific commercial product, commodity, or service in this publication is for information purposes only and does not imply indorsement by the Air Force NOTE: If you know this course contains erroneous or outdated information or does not provide the knowledge that the current Specialty Training Standard (STS) requires you to have for upgrade training, contact your unit OJT advisoi and fill out AF Form 1284, Training Quality Report. If you need immediate clarification of information in these study materials, call the author between 0700 and 1600 (CT), Monday through Friday. Consult you education officer, training officer, or NCO if you have questions on course enrollment or administration, Your Key to a Successful Course, and irregularities (possible scoring errors, printing errors, etc.) on the volume review exercises and course examination. Send questions these people can't answer to ECI, Gunter AFS AL 36118-5643, on ECI Form 17, Student Request for Assistance. This volume is valued at 45 hours (15 points) e, icni as oi April 1985. aterial in this volume is techn;,,l'y - Hi Contents Page ill Preface Cher-. Introduction to the Medical Laboratory 1 Career Field 1 Laboratory Materiel and General 2 Laboratory Safety 12 General Medical Laboratory 3 30 Administration 59 Review of Basic Chemistry 4 77 Solutions 5 Photometry and Spectrophotometry 89 6 110 Automation 7 Collection and Handling of 8 SpecimensQuality Control 118 138 Bibliography Appendixes 141 Answers for Exercises 158 NOTF: ThIg. cours# Aeadves throw** numbered lesson segments, Pitch sets 'lehavAnni objrctive, text, and exercises. The objecti- -.itwitliks yow, learning goal. The text gives you the information you need ts reach that goal, and the exercises let you check your achievement. When you complete each segment, see whether your answers match those in the back of the volume. If your response to an exercise Ls incorrect, review the objective and its text. iv 9 CHAPTER 1 Introduction to the Medical Laboratory Career Field dependents. In all instances, the analyses you perform are TO BE SUCCESSFUL, you, the laboratory technician, in the medical, indispensable if the Medical Service is to properly minister must not only possess the expertise scientific, and technical areas, but must also be able to to the health needs of those it serves. The Medical Laboratory Service is organized as a part of work effectively as managers and supervisors. If your the USAF Medical Service and is under the supervision of laboratory must provide the highest quality of service in the Surgeon General, USAF. The senior laboratory officer accomplishing the clinical laboratory mission, you must is a colonel and is an associate chief of the Biomedical prepare to accept the challenge of performing a significant Science Corps. The amount and variety of service a medical number of administrative laboratory tasks. These may laboratory provides depends upon the medical facility it supervision of range from the monitoring of training and supports. Since USAF medical facilities vary from the large laboratory personnel or the requisitioning and maintaining treatment center hospital to the smallest medical unit, so of adequate quantities of laboratory supplies and equipment does the size of medical laboratories which support them. to preparing complicated reports that are submitted to The size of the medical facility alone, however, does not higher headquarters. However, most of our administrative always dictate the nature and extent of laboratory services procedures involve preparing and maintaining accurate provided. Some laboratories have a developed fully reports and records. laboratory service, while others ship many specimens to the first section, discusses, chapter in its The other laboratories for routine or special analyses. The scope organization of the USAF Medical Laboratory Service. of treatment provided by the medical facility and the Then, to establish your role in this organization, your number, initiative, and/or training of laboratory personnel medical laboratory career field is explained. Finally, the assigned are also factors that determine the Norkload of a last section emphasizes your responsibilities and duties as a laboratory supervisor and indicates the impc:tant role you given medical laboratory. AFR 160-32, Clinical Laboratory Classification and share in the efficient performance of medical laboratory Capabilities, states that Air Force medical laboratoriec administration. categorized into four classes on the basis of iAsonnel assigned and anticipated workload. Classification of Laboratories. The four classes are 1-1. The USAF Medical Laboratory Service designated on the basis of capabilities to perform the various laboratory procedures. The medical laboratory has evolved from a basic hospital Class A. Class A laboratories are located at area medical service to an enormous business entity. The traditional role centers in the States or at the larger overseas medical of producing laboratory results to aid in the diagnosis of treatment facilities where multiple medical specialties are diseases and to follow the progress of therapy has been to give They are equipped and staffed significantly increased. Today, the laboratory has become represented. complete clinical laboratory support to the specialized provided by a very essential and visible part of the services treatment facility. They also serve as consultant, reference, the hospital. You, as a medical laboratory technician, and support laboratories to other Air Force medical perform a vital role in the accomplishment of the clinical facilities within specified geographical regions. laboratory mission. Class B. These laboratories generally are located at regional hospitals. At least one pathologist and one 001. State the mission of the USAF Medical Laboratory to biomedical laboratory officer are authorized. In ao, Service, list the factors that determine the services that a of conducting tests and examination for their medical facility, the classes and identify provides, laboratory laboratories that act as consultant, reference, and they may conduct certain tests for nearby installations when the respective hospital commanders can make satisfactory support laboratories. Class B that examinations or Tests arrant, ments. aries are not able to complete are forwarded to Class Mission of the Clinical Laboratory. The mission of the labc A laboratories or the Epidemiology Division USAF SAM or USAF Medical Laboratory Service is to provide timely and to Army or Navy laboratories in accordance with AFR in the diagnosis, laboratory support of value reliable 161-40, Joint Utilization of Certain Armed Forces Medical treatment, and prevention of diseases affecting the health and welfare of U.S. Air Force versonnel and their Laboratory Facilities. 1
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