M. Phil Dissertation Riyaz Ahmad Dar

191 Pages·2013·4.1 MB·English

M. Phil Dissertation Riyaz Ahmad Dar

PHYSIOLOGY OF SENESCENCE AND
POSTHARVEST HANDLING IN SOME SELECT
ORNAMENTAL FLOWERS OF
CARYOPHYLLACEAE

DISSERTATION SUBMITTED IN PARTIAL FULFILLMENT OF THE
REQUIREMENTS FOR THE AWARD OF
M. PHIL DEGREE IN BOTANY

By
RIYAZ AHMAD DAR
M. Sc

DEPARTMENT OF BOTANY
FACULTY OF BIOLOGICAL SCIENCES
UNIVERSITY OF KASHMIR
SRINAGAR, INDIA-190006

2013

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M. Phil Dissertation Riyaz Ahmad Dar

P.G. DEPARTMENT OF BOTANY

UNIVERSITY OF KASHMIR
HAZRATBAL, SRINAGAR, KASHMIR- 190006

No:………………..
Dated:…………….

CERTIFICATE

Certified that Mr. Riyaz Ahmad Dar has worked under my supervision for his M.
Phil. programme in Botany. The data contained in this dissertation is the
bonafide work of the candidate. The dissertation entitled “Physiology of
Senescence and Postharvest Handling in Some Select Ornamental Flowers of
Caryophyllaceae” is forwarded herewith for evaluation.

Prof. Inayatullah Tahir Prof. Irshad A.
Nawchoo
(Supervisor)

Head
Department of Botany
University of Kashmir

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M. Phil Dissertation Riyaz Ahmad Dar

DEDICATED
TO MY
PARENTS

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M. Phil Dissertation Riyaz Ahmad Dar

ACKNOWLEDGEMENTS
To begin with, I thank Almighty Allah (the most Gracious, most
Merciful) for bestowing me with enough health, dedication, courage
and patience to complete this work.
I feel privileged to express my gratitude and profound personal
regards to my supervisor Prof. Inayatullah Tahir for his constructive
criticism and enthusiastic encouragement in the preparation of this
manuscript. It has been a great pleasure to work under his guidance. I
also extend my loyal and venerable thanks to Prof. Irshad A
Nawchoo, Head Department of Botany, for providing necessary
facilities. I also take this opportunity to thank the former Head of the
Department (presently Registrar, University of Kashmir), Prof. Z. A.
Reshi for providing all the necessary facilities during the course of my
work. I am also thankful to all the teachers of the Department for
their generous help and inspiration. I express my special word of
thanks to Prof. A. Q. John and Mr. Anzar A Khuroo for correct
identification of plant material. I am also highly thankful to Mr.
Mohd Arief A. Zargar for his guidance and suggestions. I also thank
non-teaching faculty, Head Gardner and his team for their timely
suggestions and cordial support.
I appreciate the help and advice offered my senior scholars especially
Syed Sabhi Ahmad, Waseem Shahri and Sheikh Tajamul Islam during
the tenure of my research. I take this opportunity to thank Syed Sabhi
Ahmad for guiding me and being with me throughout the course of
this work. I also wish to acknowledge Shazia Nissar and Shabia Tariq
for encouragement and moral support while pursuing the present
work.
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M. Phil Dissertation Riyaz Ahmad Dar

I feel short of words to express my gratitude to my parents and my
siblings, Zahoor Ahmad Dar, Azhar udin Dar and Aabid Hussain
Dar, Rehana Shafi and Shaheena Shafi whose continuous moral
support kept me in high sprit throughout this work. I wish to
acknowledge my brethren, Ummar Ahmad, Muddasir Nazir Bhat,
Firdous Nazir Bhat and Aasif Nazir Bhat without whose blessings, I
would have never been able to complete this task.
I special note of thanks goes to my grandmother, Maam Jan (Mushtaq
Ahmad Naikoo) and my Aunt (Shareefa Banoo) for their support and
blessings. Mere words will hardly suffice the love and support
provided by them.
I am highly thankful to my friends and colleges namely Aejaz Ahmad
Dar, Syed Sabhi Ahmad, Muddasir Majeed Bhat, ,Parvaiz Ahmad
Dar, Sarwar Ahmad Pandith, Showkat Ahmad Pala, Bashir Ahmad
Mir, Shahzad Ahmad Pandith, Tariq Ahmad Paddar, Zahoor Ahmad
Itoo, Khurshid Ahmad Naikoo, Nissar Ahmad Naikoo and Farooq
Ahmad Wani for their timely suggestions, encouragement, guidance,
cooperation and moral support throughout the course of this study.

Riyaz Ahmad Dar

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M. Phil Dissertation Riyaz Ahmad Dar

LIST OF ABBREVIATIONS
ABA Abscissic acid
ACC 1-aminocyclopropane-1-caboxylic acid
ACO 1-aminocyclopropane-1-caboxylic oxidase
ACS 1-aminocyclopropane-1-caboxylic synthase
AIF Apoptosis inducing factor
AOA Aminooxyacetic acid
APX Ammonium persulphate
ATP Adenosine triphosphate
AVG Amino-ethoxyvinylglycine
BA Benzyladenine
BAP Benzyl amino purine
BSA Bovine serum albumin
CAT Catalase
cDNA Complementary deoxyribonucleic acid
CHI Cycloheximide
CoCl Cobalt choloride
2
D Day
DNA Deoxyribonucleic acid
DW Distilled water
EDTA Ethylene diamene tetra acetate
ERS Ethylene receptors sensor
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M. Phil Dissertation Riyaz Ahmad Dar

GA Gibberellic acid
3
GR Glutathione reductase
8-HQS Hydroxyquinoline sulphate
h Hour
IAA Indole acetic acid
JA Jasmonic acid
LOX Lipoxygenase
LSD Least significant difference
mRNA Messenger ribonucleic acid
NO Nitrous oxide
PA Polyamines
PAGE Polyacrylamide gel electrophoresis
PCD Programmed cell death
PCR Polymerase chain reaction
PHDS Postharvest dry storage
PHWS Postharvest wet storage
PVP Polyvinyl pyrrolidone
RH Relative humidity
RNA Ribonucleic acid
ROS Reactive oxygen species
RT Room temperature
SA Salicylic acid
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M. Phil Dissertation Riyaz Ahmad Dar

SAG Senescence associated genes
SAM S-adenosyl methionine
MDA malondialdehyde
SDS sodium dodecyl sulphate
GST glutathione S-transferase
SOD Superoxide dismutase
SPD Spermidine
SPM Spermine
STS Silver thiosulphate
Suc Sucrose
TCA Trichloroacetic acid
TEMED Tetraethylmethylenediamine
-1
Wm Watts per meter square

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M. Phil Dissertation Riyaz Ahmad Dar

Contents
Page No.
Acknowledgments
Abbreviations
Chapter 1 Introduction 1-4
Chapter 2 Review of literature 5-20
Chapter 3 Materials and Methods 21-27
Chapter 4 Flower senescence and chemical regulation of 2 8 - 7 9
vase life in Dianthus caryophyllus
Chapter 5 Flower senescence and chemical regulation 80-106
ofvase life in Dianthus barbatus
Chapter 6 Flower senescence and chemical regulation of 107-113
vase life in silene armeria
Chapter 7 Discussion 114-122
Bibliography 123-144

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M. Phil Dissertation Riyaz Ahmad Dar

CHAPTER-1

INTRODUCTION

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