LC Columns and Accessories Catalog 2014-2015

246 Pages·2013·10.51 MB·English

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C
o Thermo Scientific
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s and Consumables 2014-2015

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Your world of
chromatography
predictability
LC Columns and Accessories
As a leader in LC column technology including silica, polymer and porous graphitic
carbon manufacturing, bonded phase production and column packing for over
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4-2 35 years, you can rely on the quality of Thermo Scientific HPLC products. Here we
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s showcase our latest and most comprehensive range of innovative columns, accessories
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Section Contents C
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Introduction . . . . . . . . . . . . . . . . . . 4-001 LC Accessories. . . . . . . . . . . . . . . . 4-158
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Column Selection. . . . . . . . . . . . . . 4-002 LC Equipment. . . . . . . . . . . . . . . . . 4-186
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Column Protection . . . . . . . . . . . . . 4-016 LC Reagents. . . . . . . . . . . . . . . . . . 4-193 s

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Columns for Fast and Analytical LC . . 4-022 LC and LC-MS Instrument Parts . . 4-209
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Columns for Biomolecules. . . . . . . 4-110 Technical Information. . . . . . . . . . . 4-231 d

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Featured Products s

Accucore Columns Syncronis Columns Hypersil GOLD Columns
Ultimate core performance - Consistent, reproducible separations, Outstanding peak shape for
speed and selectivity combined column after column, time after time your separations
PAGE 4-042 PAGE 4-074 PAGE 4-024
MAbPac Columns Acclaim PepMap Columns Viper Connectors
Unrivalled resolution and effi ciency The standard for peptide Simple, dead volume free plumbing
in the analysis of protein variants separations in proteomics of HPLC and UHPLC systems
PAGE 4-126 PAGE 4-149 PAGE 4-158
www.thermoscientifi c.com/chromatography 4-001
s LC Column Selection
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Information in the following section will help you make an informed decision on the
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s appropriate HPLC column for your application, based on stationary phase use, analyte
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c properties, LC-MS requirements or USP specifications. You will also find a useful table
c of Thermo Scientific phases with specifications, as well as recommended Thermo
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Scientific alternatives for other popular columns.

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Refer to the Advanced User Graphic (AUG) on the The Pore Size is shown, with larger pore size columns Hydrophobicity
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corresponding product page (illustrated to the right) being more applicable to larger analytes such as
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Low High
m for more help and information on general purpose proteins or peptides. The percentage Carbon Load
pH Range
u column selection. is related to the hydrophobicity. Below the icon, you
ol The AUG will show you Hydrophobicity which gives will see the particle sizes available, as well as the P0ore Size 2 to 8 14
C USP code. These graphics are designed to allow you
the relative retention on the column. Generally, the
C higher the hydrophobicity, the greater the retention to quickly compare the main characteristics of multiple 0 80Å 300
Carbon Load (%)
L of neutral compounds and the higher the organic stationary phases, allowing you to choose quickly the
most appropriate stationary phase for your analysis. 0 5 25
content in the mobile phase. A lower value indicates
Particle Size2.6µm USP L11
a need for higher aqueous mobile phases to achieve For additional help in column selection, please
comparable retention and resolution. The see the back cover to contact our expert Technical
Advanced User Graphic (AUG)
recommended pH Range for the column is illustrated, Support and tap into our expertise to help make
outside of which column lifetimes will diminish. the best choice for your application.
Common HPLC Phases and Their Uses
Common Alternative Functional Normal Reverse Ion
HILIC Application
Name Name Group Phase Phase Exchange
Silica Silica -OH (cid:127) (cid:127) Non-polar and moderately polar organic compounds.
C1 SAS -(CH) Least retentive of all alkyl group bonded phases for non-polar solutes.
33 (cid:127)
Typically used for moderately polar and multi-functional compounds.
C4 Butyl -CH (cid:127) Shorter retention than C8, C18. Separation of peptides and proteins.
4 9
C8 MOS -CH Less retentive than C18; normally used for small peptides and
8 17 (cid:127)
proteins, pharmaceuticals, steroids, environmental samples.
C18 ODS -C H Most retentive of the alkyl-bonded phases. Used widely for
18 37 (cid:127)
pharmaceuticals, steroids, fatty acids, phthalates, environmental etc.
Cyano CPS, CN -(CH)CN Unique selectivity for polar compounds, more suitable than base
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silica for normal phase gradient separations. When used in reversed
(cid:127) (cid:127) phase, the selectivity is different to that of the C8 and C18 phases.
Useful for a wide range of pharmaceutical applications and for
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01 mixtures of very different solutes.
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14- Amino APS -(CH)NH HILIC: Carbohydrate analysis and other polar compounds.
s 20 23 2 (cid:127) (cid:127) (cid:127) (cid:127) Weak anion exchange: anions and organic acids.
ble Normal Phase: Alternative selectivity to silica. Good for aromatics.
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m Phenyl -(CH)CH (cid:127) Aromatic compounds and moderately polar compounds.
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n Pentafl uo- PFP -CF Extra selectivity and retention for halogenated, polar compounds
d Co rophenyl 6 5 (cid:127) and isomers.
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s a Diol -(CH2)2O (cid:127) (cid:127) (cid:127) Reversed Phase: Proteins, peptides.
mn CH2(CH2OH)2 Normal Phase: Similar selectivity to silica, but less polar.
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Col SCX Strong Cation -RSO3H- (cid:127) Organic bases.
y Exchanger
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a SAX Strong Anion -RN+(CH) Organic acids, nucleotides and nucleosides.
ogr Exchanger 33 (cid:127)
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m AX Anion Exchanger -(CHCHNH-) Organic acids, nucleotides and oligonucleotides.
hro Polyethyleneimine 2 2 n (cid:127)
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c (PEI)
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nti Porous PGC 100% carbon Particularly useful for the separation of highly polar compounds that
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ci graphitic are diffi cult to retain using conventional silica based columns;
S (cid:127) (cid:127)
o carbon separation of structurally similar compounds (e.g., isomers,
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er diastereoisomers).
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4-002
HPLC Stationary Phase Column Selection L
C
Before beginning a new analysis, consider the physical and chemical properties of the analyte(s), the mode of analysis and how the C
analyte(s) will interact with the surface of the chromatographic phase. To aid column selection, the following guide may be useful. o
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Non-polar
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Analyte
Analyte Chemical Properties Mode of Separation Recommended Phase a
Solubilty
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Acclaim Mixed-Mode WAX-1 4-069
Acidic pKa > 2 Anion exchange / Reversed-phase mixed mode Acclaim Trinity P1 4-091 A
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Accucore C18 4-045
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Accucore RP-MS 4-044 e
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Accucore C8 4-046
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Accucore C30 4-052 o
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Accucore Phenyl-Hexyl 4-049 i
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Acclaim 120 C18 4-061 s
Non-polar Reversed phase Acclaim 120 C8 4-062 >
Acclaim C30 4-064
>
AHcycplearismil PGhOeLnDy l-1 44--002663 C
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Hypersil GOLD C8 4-027 lu
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Hypersil GOLD C4 4-028 n
Neutral Syncronis C18 4-075 S
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Syncronis C8 4-076 le
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Accucore aQ 4-047 tio
Accucore Polar Premium 4-048 n
Accucore PFP 4-051
Accucore Phenyl-X 4-050
Acclaim PolarAdvantage 4-065
Polar Moderately-polar Reversed phase Acclaim PolarAdvantage II 4-066
solvents
Hypersil GOLD aQ 4-029
Hypersil GOLD PFP 4-030
Hypersil GOLD Phenyl 4-031
Syncronis aQ 4-077
Syncronis Phenyl 4-078
Accucore C18 4-045
Accucore RP-MS 4-044
Accucore C8 4-046
Accucore C30 4-052
Accucore Phenyl-Hexyl 4-049
Acclaim 120 C18 4-061
Acclaim 120 C8 4-062
Reversed phase
Acclaim C30 4-064
Basic pK < 10
a Acclaim Phenyl-1 4-063
Hypersil GOLD 4-026
Hypersil GOLD C8 4-027
Hypersil GOLD C4 4-028
Syncronis C18 4-075
Syncronis C8 4-076
Acclaim Mixed-Mode WCX-1 4-070
Cation exchange / Reversed-phase mixed mode
Acclaim Trinity P1 4-091
www.thermoscientifi c.com/chromatography 4-003
s HPLC Stationary Phase Column Selection continued
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Polar
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Analyte
c Analyte Chemical Properties Mode of Separation Recommended Phase
c Solubilty
A
Polar retention effect on graphite Hypercarb 4-084
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Hypersil GOLD AX 4-034
n Anion exchange
a Hypersil GOLD SAX 4-035
Acidic pK < 2 Accucore Urea-HILIC 4-054
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n Accucore 150-Amide-HILIC 4-146
m HILIC
Acclaim HILIC-10 4-067
u Syncronis HILIC 4-081
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o Polar retention effect on graphite Hypercarb 4-084
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Accucore HILIC 4-053

C Acclaim Mixed-Mode HILIC-1 4-068
L Acclaim Mixed-Mode WAX-1 4-069
Polar Neutral Acclaim Mixed-Mode WCX-1 4-070
solvents HILIC Acclaim Trinity P1 4-091
Hypersil GOLD Amino 4-033
Hypersil GOLD HILIC 4-037
Syncronis Amino 4-079
Syncronis HILIC 4-081
Polar retention effect on graphite Hypercarb 4-084
Acclaim Mixed-Mode WCX-1 4-070
Cation exchange
Acclaim Trinity P1 4-091
Basic pK > 10 Accucore Urea-HILIC 4-054
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Accucore 150-Amide-HILIC 4-146
HILIC
Acclaim HILIC-10 4-067
Syncronis HILIC 4-081
Hypersil GOLD Amino 4-033
Hypersil GOLD CN 4-032
Non-polar Normal phase Hypersil GOLD Silica 4-036
solvents
Syncronis Amino 4-079
Syncronis Silica 4-080
The above table recommends columns for the separation of small molecule analytes. Column recommendations for the
separation of biomolecules can be found in the table on page 4-111
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4-004
Column Format Selection for LC-MS L
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The Thermo Scientific range offers a broad array of column designs and stationary phases optimized for C
LC-MS applications. Use the following table to help you choose your column format to best meet your o
application needs. A variety of HPLC column hardware configurations are available, designed to give superior lu
results for high speed, high sensitivity, high efficiency and convenience. A wide range of particle and m
monolithic stationary phases allows choices for optimized selectivity. n
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Column Hardware Selection for LC-MS a
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LC-MS Application Column Hardware Design Description d

High throughput analysis Javelin HTS columns Direct-connection columns A
Slim design, 20mm length, 1mm to 4.6mm ID c
High sensitivity analysis Acclaim PepMap nano, capillary Nano, capillary and micro columns 0.075mm to 1mm ID; 50 to 250mm length c
and micro columns nanoViper format offers fi ngertight dead volume free connection to 1000 bar e
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Proteomics analysis EASY-Spray columns Combined column/emitter design with nanoViper connection. s
Heated fl exible silica columns 50µm and 75µm ID; 150 to 500mm length o
Acclaim PepMap nano columns, nanoViper offers fi ngertight dead volume free connection to 1000 bar ri
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Accucore nano columns, nanoViper Flexible silica columns 50μm and 75μm ID; 50 to 500mm length
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Trap column 20mm x 100μm ID
Acclaim PepMap nano columns, Flexible silica columns 50μm and 75μm ID; 50 to 500mm length >
classic Trap column 20mm x 100μm ID >
EASY-Column FTrleaxpi bcloel usmilinc a2 0comlumm xn 1s0 100μ0mm mID x 75μm C
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PepSwift monolithic columns Flexible fused silica columns, nanoViper connections lu
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100µm to 500µm ID, 50 to 250mm length
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Trap columns 5mm x 200µm ID S
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Various HPLC columns, throughout this LC section, can also be used for LC-MS application. Typical flow rates and MS source le
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compatibility for these columns are shown in the table on the next page io
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wwwwww..tthheerrmmoosscciieennttiififi cc..ccoomm//cchhrroommaattooggrraapphhyy 4-005
s Column Selection for LC-MS continued
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Packed column selection for LC-MS
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c Molecular Sample Interface Relative Column ID Flow Rate
c Weight Polarity Ionization Sensitivity (mm) (µL/min) Column Hardware
A
Low 4.6, 4.0, 3.0 2000 – 200
d Javelin HTS, Analytical
n Low APCI
a High 2.1, 1.0 200 – 50 Analytical, Javelin HTS

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n Low 4.6, 4.0, 3.0 2000 – 200 Javelin HTS, Analytical
m APCI
High 2.1, 1.0 200 – 50
u Analytical, Javelin HTS
l < 1000 Da Medium
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Low 2.1, 1.0 200 – 50
C Analytical, Javelin HTS
ESI
1.0 – 0.3 50 – 5 Acclaim PepMap (RSLC) capillary and micro
C High
0.2 – 0.05 2 – 0.2 EASY-Spray column, EASY-Column, Acclaim PepMap (RSLC) nano, Accucore nano
L
Low 2.1, 1.0 200 – 50
High Analytical, Javelin HTS
(or ionizable) ESI 1.0 – 0.3 50 – 5 Acclaim PepMap (RSLC) capillary and micro
High
0.2 – 0.05 2 – 0.2 EASY-Spray column, EASY-Column, Acclaim PepMap (RSLC) nano, Accucore nano
Low 2.1, 1.0 200 – 50
Analytical, Javelin HTS
> 1000 Da ESI
1.0 – 0.3 50 – 5 Acclaim PepMap (RSLC) capillary and micro
High
0.2 – 0.05 2 – 0.2 EASY-Spray column, EASY-Column, Acclaim PepMap (RSLC) nano, Accucore nano
Monolith columns for LC-MS
Analyte Molecular Weight Column ID (mm) Flow Rate (µL/min) Column Hardware
< 1000 Da 0.1, 0.2, 0.5 0.7 – 25 PepSwift Monolith
> 1000 Da 1.0 40 – 200 ProSwift Monolith
ProSwift is also available in larger IDs for high throughput applications.
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4-006
HPLC Column Selection by U.S. Pharmacopeia Specifi cations* L
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USP Code Description Recommended Phase Page
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L1 Octadecyl silane (C18) chemically bonded to porous Acclaim 120 C18 4-061 l
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or ceramic microparticles, 1.5 to 10μm in diameter,
Acclaim 300 C18 4-142 m
or a monolithic rod
Accucore C18 4-045 n
Accucore aQ 4-047 s

Accucore 150-C18 4-144 a
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Accucore XL C18 4-056
d
BioBasic 18 4-140
A
Hypersil GOLD 4-026
c
Hypersil GOLD aQ 4-029 c
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Acclaim PepMap 100 C18 4-149
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Syncronis C18 4-075 s
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Syncronis aQ 4-077
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L3 Porous silica particles, 1.5 to 10μm in diameter, Accucore HILIC 4-053 ie
or a monolithic rod Hypersil GOLD Silica 4-036 s
Syncronis Silica 4-080 >
L7 Octyl silane chemically bonded to totally Acclaim 120 C8 4-061 >
o1.r5 s tuop 1e0rfiμ cmia ilnly d piaomroeutse sr,i loicra a p maortnicolleitsh, ic rod Accucore C8 4-046 C
Accucore XL C8 4-057 o
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BioBasic 8 4-140 m
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Hypersil GOLD C8 4-027 S
Acclaim PepMap 100 C8 4-150 e
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Syncronis C8 4-076 c
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L8 An essentially monomolecular layer of Hypersil GOLD Amino 4-033 io
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aminopropylsilane chemically bonded to totally
Syncronis Amino 4-079
porous silica gel support, 1.5 to 10μm in diameter
L10 Nitrile groups chemically bonded to porous silica Hypersil GOLD CN 4-032
particles, 1.5 to 10μm in diameter
L11 Phenyl groups chemically bonded to porous silica Accucore Phenyl-Hexyl 4-049
particles, 1.5 to 10μm in diameter
Hypersil GOLD Phenyl 4-031
Syncronis Phenyl 4-078
L13 Trimethylsilane chemically bonded to porous silica BETASIL C1 Inquire
particles, 3 to 10μm in diameter
Hypersil SAS 4-107
L14 Silica gel having a chemically bonded, strongly basic Hypersil GOLD SAX 4-035
quaternary ammonium anion exchange coating,
Hypersil SAX 4-109
5 to 10μm in diameter
L15 Hexylsilane (C6) chemically bonded to totally porous BETASIL C6 Inquire
silica particles, 3 to 10μm in diameter
www.thermoscientifi c.com/chromatography 4-007
s HPLC Column Selection by U.S. Pharmacopeia Specifi cations* continued
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USP Code Description Recommended Phase Page
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c L17 Strong cation exchange resin consisting of sulfonated HyperREZ XP Carbohydrate H+ 4-156
c cross-linked styrene-divinylbenzene copolymer in the
HyperREZ XP Organic Acids 4-156
A hydrogen form, 6 to 12μm in diameter
d L19 Strong cation exchange resin consisting of sulfonated HyperREZ XP Carbohydrate 4-156
n cross-linked styrene-divinylbenzene copolymer in the Ca2+
a calcium form, about 9μm in diameter HyperREZ XP Sugar Alcohols 4-156

s L20 Dihydroxypropane groups chemically bonded to BETASIL Diol Inquire
n porous silica or hybrid particles, 1.5 to 10μm
m in diameter
u L26 Butyl silane chemically bonded to totally porous silica Accucore 150-C4 4-145
ol particles, 1.5 to 10μm in diameter BioBasic 4 4-140
C
Hypersil GOLD C4 4-028

C Acclaim PepMap 300 C4 4-149
L L33 Packing having the capacity to separate dextrans BioBasic SEC 120 4-139
by molecular size over a range of 4,000 to 500,000
BioBasic SEC 300 4-139
daltons. It is spherical, silica-based, and processed
to provide pH stability BioBasic SEC 1000 4-139
MAbPac SEC-1 4-129
L34 Strong cation exchange resin consisting of sulfonated HyperREZ XP Carbohydrate Pb2+ 4-156
cross-linked styrene-divinylbenzene copolymer in the
lead form, 7 to 9μm in diameter
L43 Pentafl uorophenyl groups chemically bonded to silica Accucore PFP 4-051
particles by a propyl spacer, 1.5 to 10μm in diameter Hypersil GOLD PFP 4-030
L46 Polystyrene/divinylbenzene substrate agglomerated OmniPac PAX-100 4-103
with quaternary amine functionalized latex beads
about 9 to 11um in diameter
L50 Multifunction resin with reverse-phase retention and OmniPac PAX-500 4-103
strong anion-exchange functionalities. The resin
consists of ethylvinylbenzene, 55% cross-linked with
divinylbenzene copolymer, 3 to 15µm in diameter, and
a surface area of not less than 350m2 per g.
Substrate is coated with quaternary ammonium
functionalized latex particles consisting of styrene
cross-linked with divinylbenzene
L52 A strong cation exchange resin made of porous silica BioBasic SCX 4-141
with sulfopropyl groups 5 to 10μm in diameter
L58 Strong cation exchange resin consisting of sulfonated HyperREZ XP Carbohydrate Na+ 4-156
cross-linked styrene-divinylbenzene copolymer in the
sodium form, about 6 to 30μm in diameter
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01 L59 Packing for the size exclusion separation of proteins BioBasic SEC 300 (5μm) 4-139
14-2 (separation by molecular weight) over the range of MAbPac SEC-1 4-129
0 5 to 7,000 kDa. The packing is spherical, 1.5 to 10um,
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s silica or hybrid packing with a hydrophilic coating
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a L60 Spherical, porous silica gel, 10μm or less in diameter, Acclaim PolarAdvantage 4-065
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u the surface of which has been covalently modifi ed
s Acclaim PolarAdvantage II 4-066
on with alkyl amide groups and endcapped
d C Accucore Polar Premium 4-048
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s a L62 C30 silane bonded phase on a fully porous spherical Acclaim C30 4-064
mn silica, 3 to 15µm in diameter Accucore C30 4-052
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Col L78 A silane ligand that consists of both reversed-phase Acclaim Mixed-Mode WAX-1 4-069
hy (an alkyl chain longer than C8) and anion-exchange
p
gra (primary, secondary, tertiary or quaternary amino
ato groups) functional groups chemically bonded to
m porous or non-porous or ceramic micro-particles,
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hr 1.0 to 50μm in diameter or a monolithic rod
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fi * These are the recommended Thermo Scientific HPLC columns for various USP categories although other columns
enti for each category are also available.
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4-008