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Kato-Katz technique Advantages PDF

155 Pages·2011·9.3 MB·English
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Preview Kato-Katz technique Advantages

Laura Rinaldi and Giuseppe Cringoli Dept. Pathology and Animal Health, University of Naples Federico II, Naples, Italy Parasitological Diagnosis for STH and other parasites Copromicroscopic techniques COPROMICROSCOPIC TECHNIQUES AND PARASITIC ELEMENTS DETECTABLE Techniques Parasitic elements Eggs Oocysts Cysts Larvae Direct Smear O O O O Simple Flotation in tube O O O Flotation in centrifuge (Wisconsin) O McMaster O O Mes method O Sedimentation O Baermann O Kato Katz O O O Ether/ethyl acetate concentration O O O FLOTAC O O O O Quantitative techniques = O Qualitative techniques = O Helminths - Protozoa COPROLOGICAL examination broncopolmonari Gastro-intestinal tract - Liver, lungs Sensu stricto EGGS LARVAE OOCYSTS CYSTS Ab DNA Sensu lato Helminthsand protozoa which inhabitthe GI tract and lungs of the hosts eliminate their eggs/larvae with the faecesof hosts STH Ascaris lumbricoides Trichuris trichiura Ancylostoma duodenale and Necator americanus Faecal Egg Count (FEC) techniques are considered relatively straightforward and protocols such as the Kato-Katz technique and the ether-based concentration method in the human field have been available for many years. Disadvantages Advantages Kato-Katz technique -Small amount of feces exam. - Simplicity and low cost (usually 41.7 mg) and thus low analytic sensitivity (24 EPG) - Fresh samples Low sensitivity and accuracy Ether-based Disadvantages Advantages concentration - Eggs can be broken or altered - Stool samples preserved - Reference laboratory - Diagnosis of both - Qualitative helminths and protozoa Multivalent techniques characterized by high sensitivity, specificity, accuracy

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COPROMICROSCOPIC TECHNIQUES AND PARASITIC ELEMENTS the Kato-Katz technique and the ether-based concentration method in the
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