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TUI^ STUDIES ON POST HARVEST SHELF LIFE AND PROCESSING OF RED FLESHED GUAVA (Psidium guajava L.) BY KADAM ADITYA RAJENDRA M. Sc. (Hort.) DISSERATION Submitted to the Vosontroo NmbJMomtfcwadd %jisfd Vidyapeeth, <Par6ham in panMfulfillment eft fie requirements Jbrtfie (Degree of DOCTOR OF PHILOSOPHY (Horticulture) IN HORTICULTURE DEPARTMENT OF HORTICULTURE COLLEGE OF AGRICULTURE, PARBHANI VASANTRAO NAIK MARATHWADA KRISHIVIDYAPEETH, PARBHANI - 431 402 (M.S.) INDIA 2015 CANDIDATE’S DECLARATION I hereby declare that this dissertation or part thereof has not been previously submitted by me to any other university or institution for a degree or diploma - Place: PARBHANI Date lOa/oaiXdlf (KADAMA.R.) Dr. V. S. KHANDARE Associate Professor, Department of Horticulture, Vasantrao Naik Marathwada KrisM Vidyapeeth, Parbhani. 431 402 (M.S.) India. CERTIFICATE -1 This is to certify that the dissertation entitled “STUDIES ON POST HARVEST SHELF LIFE AND PROCESSING OF REDFLESHED GUAVA (Psidium guajava Linn.)” submitted by MR. KADAM ADITYA RAJENDRA to the Vasantrao Naik Marathwada Krishi Vidyapeeth Parbhani, in partial fulfillment of the requirement of the degree of DOCTOR OF PHILOSOPHY in the subject of HORTICULTURE is a record of original bonafide research work prosecuted by him under my guidance and supervision. The dissertation, in my opinion is of sufficiently high standard to warrant it’s presentation for the award of the said degree. I also certify that the dissertation or part thereof has not been previously submitted by him for any degree, diploma or distinction to any other university/institution. The assistance and help rendered during the course of investigation and sources of literature have been duly acknowledged. Place: PARBHANI Research Guide Date CERTIFICATE - II This is to certify that the dissertation entitled “STUDIES ON POST HARVEST SHELF LIFE AND PROCESSING OF RED FLESHED GUAVA {Psidium guajava Linn.)” submitted by MR. KADAM ADITYA RAJENDRA to the Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.) in partial fulfillment of the requirement for the degree of DOCTOR OF PHILOSOPHY in the subject of HORTICULTURE has been approved by the student's advisory committee after viva-voce examination in collaboration with the external examiner. Advisory Committee: (T. B. Tambe) Associate Dean (P.G.) College of Agriculture, V.N.M.K.V. Parbhani 431 402 (M.S.). ACKNOWLEDGEMENT In the difficult path of my academic journey many people showed the way towards success. I think it is the matter of pleasure to glance back and recall the way one traverse, the days ofhardwork and perseverance. It. is still great at the juncture to recall all the faces and spirits in the form of teachers, friends, near and dear. In my opinion, this work is nothing more than incomplete, without attending to the task acknowledging, to overwhelming help I received during this endeavour of mine. Inspiration is the best medicine which can make it possible to run for crippled one and it is veritable gold of mine to get the talented and inspiring willful guidance of Dr. V.S. Khandare, PhD. (AgrL) Associate Professor, Department of Horticulture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani and Chairman of my advisory committee, who in his unique way, provided me with constant encouragement inspiring, scholastic guidance, love and affection offered to me during the course of my study and research Words would be a poor vehicle to communicate him my sense of gratitude. I am also thankful to Dr. T.B. Tambe, Officer Incharge, FRS, Aurangabad for providing fascinating research topic and valuable guide. It is incumbent upon me to express my profound sense of gratitude to Dr. G.M. Waghmare, Head Department of Horticulture, Dr. V.N. Pawar, Dr. Md. Asmatoddin, Ex-Associate Professor Department of Agril Econ. And Stat. V.N.M.K.U., Parbhani who have been instrumental and assertive in the materialization of the research project and bring to this shape. Prof. DM. Naik, Dr. S.J. Shinde, Prof. S.S. Yadlod, Dr. A.M. Bhosale, Prof. B.M. Kalalbandi and all staff members of Department of Horticulture, V.N.M.K.U., Parbhani may be regarded as the light hours for the ocean liners who have been kindly navigated my ship of academic pursuit and I would like to mention my gratitude to them. I express my sincere thanks to the superintendent Shri. Samale, Shri. Ramble and his colleagues Bhagwan, Mauli, Marotrao, Jodhale for their help during research work. Special thanks to Shri. Kalyan Kaldate for his help during work. Thanks is the worst word in friendship, yet I shall avail this opportunity to extend my sincere heartfelt gratitude to my dear friends Rinku sir, Rajendra, Rajdeep, Subodh, Mdhesh, Nitin, Nandu Sir, Amol, Anil, Yogesh, Mangesh, Abhijit, Madan, Maruti, Shivprasad, Ajit, Kishor Ganesh, Arjun, Akshay, Krishna and Avinash who have all the way co-operated during the course of my study and also acknowledge the moral and material help extended to me by all my friends. No words are enough to express the great sacrifice, devotion, constant encouragement and inspiration of my beloved, respected and adorable father Shri. Rajendra Nanaji Kadam and mother Sau. Asha Rajendra Kadam without my father and mother moral support, affection and guidance. I would not have successful in this difficult endeavor of post graduate studies. I shall fail in my duties if I don’t place on record my heartful gratitude to my brother Indrajeet and Gaurav, for their love, moral support, encouragement and sharing cheerful in the difficulties encountered during my educational life and building up my career. Last but not least, my special thanks to whose immense help made me possible to complete this manuscript. Lastly I thank all my friends and relatives whose names are not listed above, but who have helped me directly or indirectly in the preparation of this manuscript. Place: Parbhani Date (KADAM A.R.) CONTENTS Chapter Title Page No. No. I. INTRODUCTION 1-5 n. REVIEW OF LITERATURE 6-45 l in. MATERIALS AND METHODS 46-57 IV. RESULTS 58-94 V. DISCUSSION 95-105 VI. SUMMARY AND CONCLUSION 106-108 LITERATURE CITED » i-xv APPENDIX I • LIST OF TABLES Table Page Title No. No. Physicochemical composition and antioxidant composition 1 58 of red fleshed guava fruit Effect of 1-MCP and chitosan coating on physiological loss 2 in weight of red fleshed guava during storage at ambient 60 temperature and low temperature (10°C) Effect of 1-MCP and chitosan coating on firmness of red 3 fleshed guava during storage at ambient temperature and 62 low temperature (10°C) Effect of 1-MCP and chitosan coating on decay of red 4 fleshed guava during storage at ambient temperature and 64 low temperature (10°C) Effect of 1-MCP and chitosan coating on respiration rate of 5 red fleshed guava during storage at ambient temperature 66 and low temperature (10°C) Effect of 1-MCP and chitosan coating on ethylene 6 evaluation of red fleshed guava during storage at ambient 68 temperature and low temperature (10°C) Effect of 1-MCP and chitosan coating on total soluble 7 solids of red fleshed guava during storage at ambient 70 temperature and low temperature (10°C) Effect of 1-MCP and chitosan coating on titratable acidity 8 of red fleshed guava during storage at ambient temperature 72 and low temperature (10°C) Effect of 1-MCP and chitosan coating on ascorbic acid of 9 red flesh guava during storage at ambient temperature and 74 low temperature (10°C) Effect of 1-MCP and chitosan coating on anthocyanin 10 content of red fleshed guava during storage at ambient 76 temperature and low temperature (10°C) Effect of l-MCP and chitosan coating on total phenol 11 content of red fleshed guava during storage at ambient 78 temperature and low temperature (10°C) Effect of 1-MCP and chitosan coating on flavonoid content 12 of red fleshed guava during storage at ambient temperature 80 and low temperature (10°C) Effect of 1-MCP and chitosan coating on antioxidant 13 activity of red fleshed guava by FRAP assay during storage 83 at ambient temperature and low temperature (10°C) Effect of 1-MCP and chitosan coating on antioxidant 14 activity of red fleshed guava by CUPRAC assay during 85 storage at ambient temperature and low temperature (10°C) Effect of processing on physico-chemical and antioxidant 15 88 activities of red fleshed guava pulp Effect of processing and days of storage on physic-chemical composition of guava pulp at ambient temperature and cold 16 91 storage Effect of processing and days of storage on antioxidant 17 composition of guava pulp at ambient temperature and cold 91 storage Effect of processing and days of storage on antioxidant activity of guava pulp at ambient temperature and cold 18 92 storage Effect of processing on chromacity of red fleshed guava 19 94 pulp during storage at ambient temperature and cold storage LIST OF FIGURES In Fig. Title betwee No. n Pages 1 Extraction of red fleshed guava pulp 50 Effect of 1-MCP and chitosan coating on sensory quality 2 of red fleshed guava during storage at ambient 86-87 temperature Effect of 1-MCP and chitosan coating on sensory quality 3 of red fleshed guava at 10th day during storage at 10° C 86-87 temperature. Effect of 1-MCP and chitosan coating on sensory quality 4 of red fleshed guava at 20th day during storage at 10° C 86-87 temperature

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Effect of 1-MCP and chitosan coating on anthocyanin .. steadily in control fruit, and the combination of MAP and 1 MCP significantly reduced The total flavonoid content (TFC) showed the same trend where the leaves showed . marked changes in pectin and cloud substances and
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