VOLUME 44 - CONTENTS No 1 JANUARY 1991 Page Occasionals 1 Current probiems in microbiology: 1 Chlamydial infections: Which laboratory test? G L Ridgway, D Taylor-Robinson 6 Survey of users’ attitudes to their local microbiology laboratory S J Pedler, A J Bint Papers 10 Audit in histopathology: Description of an internal quality assessment scheme with analysis of preliminary results J A Zuk, W E Kenyon, M W Myskow 17 Continuous assessment of performance in External Quality Assessment Schemes J C Ardern, K Hyde, L Hickman, et al 21 Hepatocyte expression of HBcAg and serum HBeAg in hepatitis B: Comparison of polyclonal and monoclonal antibodies during a trial of interferon C-M Chu, S-M Lin, Y-F Liaw 25 Evaluation of the pre-S (pre-S(1)Ag/pre-S(2)Ab) system in hepatitis B virus infection M I Galan, J Tomas, M C Bernal, F J Salmerén, M C Maroto 29 Endothelial markers in malignant vascular tumours of the liver: Superiority of QB-END/10 over Von Willebrand factor and Ulex europaeus agglutinin 1 P P Anthony, P Ramani 33 Interphase cytogenetics using biotin and digoxigenin labelled probes: III Increased sensitivity and flexibility for detecting HPV in cervical biopsy specimens and cell lines C S Herrington, A K Graham, J O’D McGee 39 Monocytoid B cell lymphoma S S Banerjee, M Harris, B P Eyden, J A Radford, C J Harrison, A R Mainwaring 45 Screening for lupus anticoagulant and anticardiolipin antibodies in women with fetal loss M D Creagh, R G Malia, S M Cooper, A R Smith, S L B Duncan, M Greaves 48 Chromosomes in childhood acute lymphoblastic leukaemia: Karyotypic patterns in disease subtypes MA Wodzinski, A E Watmore, J S Lilleyman, A M Potter 52 Breakdown of gastric mucus in presence of Helicobacter pylori R L Sidebotham, J J Batten, Q N Karim, J Spencer, J H Baron 58 Evaluation of Clinitek 200 and Rapimat I1I/T for screening for urinary tract infection R A Bowman, T V Riley 61 Measurement of urinary lipopolysaccharide antibodies by ELISA as a screen for urinary tract infection A P MacGowan, R J Marshall, P Cowling, D S Reeves 64 Serum non-organ specific autoantibodies in human immunodeficiency virus 1 infection F Cassani, L Baffoni, E Raise, et al 69 Immunoassay of P2 protein in cerebrospinal fluid in neurological disorders J Colover 73 Increased serum £,-microglobulin concentrations in hyperthyroid states J Roiter, G da Rin, E de Menis, GC Foscolo, P Legovini, N Conte Short reports Monoclonal i fluorescence compared with silver stain for investigating Pneumocystis carinii pneumonia J Midgley, P A Parsons, D C Shanson,O A N Husain, N Francis Detection of chloramphenicol resistance in Haemophilus influenzae J J S Snell, S F Perry, D F J Brown Retrospective application of prognostic indices to pancreatitis discovered at necropsy M K Heatley HLA antigens in Hungarian patients with idiopathic haemochromatosis E Czink, E K Gyédi, K Német, S Hollan Cytogenetic analysis of a granulocytic sarcoma in a patient without systemic leukaemia L R Adam, B Angus, P Carey, E V Davison Comparison of two commercial kits for identifying and biotyping Haemophilus parainfluenzae M Warren, Z Ahmet, E Houang Miscellanea Correspondence - Book reviews - Notices - Corrections No 2 FEBRUARY 1991 Occasionals Laboratory handling of impalpable breast lesions: A review J S Armstrong, J D Davies Early clinical pathologists: 3 Sidney Campbell Dyke E K Blackburn Papers Simultaneous in situ genotyping and phenotyping of human papillomavirus cervical lesions: Comparative sensitivity and specificity A K Graham, C S Herrington, J O’D McGee Macrophages, microglial cells, and HLA-DR antigens in fetal and infant brain M M Esiri, M S Al Izzi, MC Reading Class I and class II major histocompatibility complex antigens on hepatocytes: Importance of the method of detection and expression in histologically normal and diseased livers G Senaldi, A Lobo- Yeo, A P Mowat, G Mieli-Vergani, D Vergani Simplified procedures for applying the polymerase chain reaction to routinely fixed paraffin wax sections P J Coates, A J d’ Ardenne, G Khan, H O Kangro, G Slavin Twelve cases of Ki-1 positive anaplastic large cell lymphoma of skin S S Banerjee, J Heald, M Harris Increased central immunoreactive B-endorphin content in patients with Wernicke-Korsakoff syndrome and in alcoholics J A Summers, P T Pullan, J J Kril, C G Harper Comparison of a modified thiazole orange technique with a fully automated analyser for reticulocyte counting D Bowen, N Bentley, T Hoy, I Cavill Globin chain synthesis in myelodysplastic syndromes G Chalevelakis, S Karaoulis, A G Yalouris, Th Economopoulos, N Tountas, S Raptis Distribution of plasminogen activator inhibitor (PAI-1) in tissues A J Simpson, N A Booth, N R Moore, B Bennett I histochemical analysis of amylase isoenzymes in thyroid cancer M Higashiyama, S Doi, N Tomita, et al Comparison of image analysis and flow cytometric determination of cellular DNA content C Cope, D Rowe, L Delbridge, J Philips, M Friedlander Immunoblotting and culture positive endocarditis J Clark, J P Burnie Harnessing of urease activity of Helicobacter pylori to induce self-destruction of the bacterium M A Greig, W D Neithercut, M Hossack, K E L McColl Incidence of penicillin tolerance among blood culture isolates of Streptococcus sanguis, 1987-88 P A James, S E J YounD gG W,hi te VOLUME 44 - CONTENTS No 1 JANUARY 1991 Page Occasionals 1 Current probiems in microbiology: 1 Chlamydial infections: Which laboratory test? G L Ridgway, D Taylor-Robinson 6 Survey of users’ attitudes to their local microbiology laboratory S J Pedler, A J Bint Papers 10 Audit in histopathology: Description of an internal quality assessment scheme with analysis of preliminary results J A Zuk, W E Kenyon, M W Myskow 17 Continuous assessment of performance in External Quality Assessment Schemes J C Ardern, K Hyde, L Hickman, et al 21 Hepatocyte expression of HBcAg and serum HBeAg in hepatitis B: Comparison of polyclonal and monoclonal antibodies during a trial of interferon C-M Chu, S-M Lin, Y-F Liaw 25 Evaluation of the pre-S (pre-S(1)Ag/pre-S(2)Ab) system in hepatitis B virus infection M I Galan, J Tomas, M C Bernal, F J Salmerén, M C Maroto 29 Endothelial markers in malignant vascular tumours of the liver: Superiority of QB-END/10 over Von Willebrand factor and Ulex europaeus agglutinin 1 P P Anthony, P Ramani 33 Interphase cytogenetics using biotin and digoxigenin labelled probes: III Increased sensitivity and flexibility for detecting HPV in cervical biopsy specimens and cell lines C S Herrington, A K Graham, J O’D McGee 39 Monocytoid B cell lymphoma S S Banerjee, M Harris, B P Eyden, J A Radford, C J Harrison, A R Mainwaring 45 Screening for lupus anticoagulant and anticardiolipin antibodies in women with fetal loss M D Creagh, R G Malia, S M Cooper, A R Smith, S L B Duncan, M Greaves 48 Chromosomes in childhood acute lymphoblastic leukaemia: Karyotypic patterns in disease subtypes MA Wodzinski, A E Watmore, J S Lilleyman, A M Potter 52 Breakdown of gastric mucus in presence of Helicobacter pylori R L Sidebotham, J J Batten, Q N Karim, J Spencer, J H Baron 58 Evaluation of Clinitek 200 and Rapimat I1I/T for screening for urinary tract infection R A Bowman, T V Riley 61 Measurement of urinary lipopolysaccharide antibodies by ELISA as a screen for urinary tract infection A P MacGowan, R J Marshall, P Cowling, D S Reeves 64 Serum non-organ specific autoantibodies in human immunodeficiency virus 1 infection F Cassani, L Baffoni, E Raise, et al 69 Immunoassay of P2 protein in cerebrospinal fluid in neurological disorders J Colover 73 Increased serum £,-microglobulin concentrations in hyperthyroid states J Roiter, G da Rin, E de Menis, GC Foscolo, P Legovini, N Conte Short reports Monoclonal i fluorescence compared with silver stain for investigating Pneumocystis carinii pneumonia J Midgley, P A Parsons, D C Shanson,O A N Husain, N Francis Detection of chloramphenicol resistance in Haemophilus influenzae J J S Snell, S F Perry, D F J Brown Retrospective application of prognostic indices to pancreatitis discovered at necropsy M K Heatley HLA antigens in Hungarian patients with idiopathic haemochromatosis E Czink, E K Gyédi, K Német, S Hollan Cytogenetic analysis of a granulocytic sarcoma in a patient without systemic leukaemia L R Adam, B Angus, P Carey, E V Davison Comparison of two commercial kits for identifying and biotyping Haemophilus parainfluenzae M Warren, Z Ahmet, E Houang Miscellanea Correspondence - Book reviews - Notices - Corrections No 2 FEBRUARY 1991 Occasionals Laboratory handling of impalpable breast lesions: A review J S Armstrong, J D Davies Early clinical pathologists: 3 Sidney Campbell Dyke E K Blackburn Papers Simultaneous in situ genotyping and phenotyping of human papillomavirus cervical lesions: Comparative sensitivity and specificity A K Graham, C S Herrington, J O’D McGee Macrophages, microglial cells, and HLA-DR antigens in fetal and infant brain M M Esiri, M S Al Izzi, MC Reading Class I and class II major histocompatibility complex antigens on hepatocytes: Importance of the method of detection and expression in histologically normal and diseased livers G Senaldi, A Lobo- Yeo, A P Mowat, G Mieli-Vergani, D Vergani Simplified procedures for applying the polymerase chain reaction to routinely fixed paraffin wax sections P J Coates, A J d’ Ardenne, G Khan, H O Kangro, G Slavin Twelve cases of Ki-1 positive anaplastic large cell lymphoma of skin S S Banerjee, J Heald, M Harris Increased central immunoreactive B-endorphin content in patients with Wernicke-Korsakoff syndrome and in alcoholics J A Summers, P T Pullan, J J Kril, C G Harper Comparison of a modified thiazole orange technique with a fully automated analyser for reticulocyte counting D Bowen, N Bentley, T Hoy, I Cavill Globin chain synthesis in myelodysplastic syndromes G Chalevelakis, S Karaoulis, A G Yalouris, Th Economopoulos, N Tountas, S Raptis Distribution of plasminogen activator inhibitor (PAI-1) in tissues A J Simpson, N A Booth, N R Moore, B Bennett I histochemical analysis of amylase isoenzymes in thyroid cancer M Higashiyama, S Doi, N Tomita, et al Comparison of image analysis and flow cytometric determination of cellular DNA content C Cope, D Rowe, L Delbridge, J Philips, M Friedlander Immunoblotting and culture positive endocarditis J Clark, J P Burnie Harnessing of urease activity of Helicobacter pylori to induce self-destruction of the bacterium M A Greig, W D Neithercut, M Hossack, K E L McColl Incidence of penicillin tolerance among blood culture isolates of Streptococcus sanguis, 1987-88 P A James, S E J YounD gG W,hi te Short reports What’s in a burger? N J Carr, L G Machin The need for primary quality control of commercially available immunoassay kits T Hashimoto, F Matsubara Helicobacter pylori serology using specimens collected on filter paper Z Huang, B Dwyer, J Kaldor Laboratory detection of ciprofloxacin resistant Neisseria gonorrhoeae A Turner, A E Jephcott, K R Gough Examination of spinal cord in diseases of the craniocervical junction and high cervical spine J F Geddes, A G Gonzalez Royal College of Pathologists’ accreditation pilot study: a year later The College Audit Steering Committee Miscellanea Correspondence - Book reviews - Notices - Corrections No 3 MARCH 1991 Occasionals In situ hybridisation in perspective A Warford, I Lauder Opportunistic protozoan infections in human immunodeficiency virus disease: Review highlighting diagnostic and therapeutic aspects A Curry, A J Turner, S Lucas Papers Histopathology and i histochemistry of the caecum in children with the Trichuris dysentery syndrome 4 T MacDonald, M-Y Choy, J Spencer, et al histological characterisation of amyloid deposits in renal biopsy specimens R J Fitzmaurice, C Bartley, ;M cClure, P Ackrill Occult papillary microcarcinoma of the thyroid — a potential pitfall of fine needle aspiration cytology? H R Harach, E Saravia Day, S B Zusman Ki-67 staining in histological subtypes of breast carcinoma and fine needle aspiration smears V Kuenen-Bloumeester, Th H Van Der Kwast, H A J Van Laarhoven, S C Henzen-Logmans c-erbB-2 expression in different histological types of invasive breast carcinoma S Soomro, S Shousha, P Taylor, H M Shepard, M Feldmann DNA flow cytometry of follicular non-Hodgkin’s lymphoma J C Macartney, R S Camplejohn, R Morris, K Hollowood, D Clarke, A Timothy Histometric studies on cellular infiltrates of tuberculin tests in patients with sarcoidosis J G Lowe, J H Gibbs, RC Potts, J L Stanford, J Swanson Beck Monocyte counting: Discrepancies in results obtained with different automated instruments W Goossens, L van Hove, R L Verwilghen Measurement of acute phase proteins for assessing severity of Plasmodium falciparum malaria S H Gillespie, C Dow, J G Raynes, R H Behrens, P L Chiodimi, K P W J McAdam Plasma leucocyte elastase concentrations in smokers C R K Hind, H Joyce,G A Tennent, M B Pepys, N B Pride Measurement of alkaline phosphatase of intestinal origin in plasma by P-bromotetramisole inhibition T Kuwana, S B Rosalki Automated microparticle enzyme immunoassays for IgG and IgM antibodies to Toxoplasma gondii J W Safford, G G Abbott, M C Craine, RG MacDonald Plasma histamine in patients with chronic renal failure and nephrotic syndrome D S Gill, V A Fonseca, M A Barradas, R Balliod, J F Moorhead, P Dandona Lack of specificity for antibodies to double stranded DNA found in four commercial kits M Kadlubowski, M Jackson, P L Yap, G Neill Short reports Immunocytological diagnosis of primary cerebral non-Hodgkin’s lymphoma A P Lai, A S Wierzbicki, P M Norman Primary squamous cell carcinoma of the terminal ileum C C Platt, N Y Haboubi, P F Schofield Cells containing factor XIIIa and pulmonary fibrosis induced by bleomycin M Toida, Y Okumura, T Takami Audit of turn-around times in a microbiology laboratory S Rogers, M J Bywater, D S Reeves Comparative yield of Salmonella typhi from blood and bone marrow cultures in patients with fever of unknown origin B J Farooqui, M Khurshid, M K Ashfaq, M Ata Khan Recovery ef CD3+ and CD5— lymphocyte subpopulation after autologous bone marrow transplantation and chemotherapy P J McKay, A McLaren, L M Andrews, N P Lucie Miscellanea Correspondence - Book reviews - Notices - No 4 APRIL 1991 Occasionals Inadequacy of death certification: Proposal for change T G Ashworth ACP Broadsheet 127 April 1991: Measurement of catecholamines and their metabolites in urine C Weinkove Papers Ophthalmomyiasis caused by the reindeer warble fly larva M S Kearney, A C Nilssen, A Lyslo, P Syrdalen, L Dannevig Expression of MHC class II antigens by conjunctival epithelial cells in trachoma: Implications concerning the pathogenesis of blinding disease D C W Mabey, R L Bailey, D Dunn, et al Human cervical epithelial cells that express HLA-DR associated with viral infection and activated mononuclear cell Panne Ss Fais, F Delle Fratte, F Mancini, et al istochemical demonstration of CD 23 expression on lymphocytes in rheumatoid synovitis E A Hellen, D C Rowlands, T T Hansel, G D Kitas, J Crocker Influenza A and the virus associated haemophagocytic syndrome: Cluster of three cases in children with acute leukaemia M N Potter, A B M Foot, A Oakhill Dysmegakaryopoiesis in myelodysplastic syndromes (MDS): An i ymorphometric study of bone marrow trephine biopsy specimens J Thiele, H Quitmann, S Wagner, R Fischer se of plasma selectively depleted in fibrinogen by affinity chromatography C F Hirst, K J Stevenson hemical demonstration of neurone specific enolase in bone marrow infiltrated by neuroblastoma JM Caillaud, F Martinez-Madrigal, O Hartmann, C Carlu Use of electron microscopy in examination of faeces and rectal and jejunal biopsy specimens G M M Connolly, DS Ellis, J E Williams, G Tovey, B G Gazzard Congenital mesoblastic neophroma: Possibie prognostic and management value of assessing DNA content J C Barrantes, C Toyn, K R Muir, et al Detection of Chlamydia trachomatis by the polymerase chain reaction in swabs and urine from men with non- gonococcal urethritis H M Palmer, C B Gilroy, B J Thomas, et al Evaluation of a commercial ELISA for serodiagnosis of Helicobacter pylori infection J E Crabtree, T M Shalicross, R V Heatley, J I Wyatt Use of rapid carbohydrate utilisation test for identifying “Streptococcus milleri group” J M Whitworth, P W Ross, IR Poxton A silver enhanced gold labelled immunosorbent assay for detecting antibodies to rubella virus N Patel, B F Rocks, M P Bailey Short reports Listeria meningitis and paté P M Cumber, W Mumar-Bashi, S Palmer, R D Hutton Absence of human papillomavirus in cervical adenocarcinoma determined by in situ hybridisation F J Young, L M Ward, L J R Brown Persistent polyclonal B lymphocytosis with Epstein-Barr virus antibodies and subsequent malignant pulmonary blastoma E Lawlor, M Murray, D S O’ Briain, et al Lack of C-erbB-2 protein expression in pulmonary carcinoid tumours N Wilkinson, P S Hasleton, S Wilkes, A Quigley Chronic myelomonocytic leukaemia associated with T cell receptor 6 gene rearrangement A B Provan, R V Majer, AG Smith, B Wilkins, E Hodges, J L Smith Strongyloides stercoralis hyperinfection in an HIV positive patient J N Harcourt-Webster, F Scaravilli, A H Darwish Miscellanea Correspondence - Book reviews - Notices - No 5 MAY 1991 Occasionals Locally organised medical audit in histopathology A D Ramsay Self-infusion with immunoglobulin at home H Chapel, V Brennan Papers Inhibitory effects of urine on the polymerase chain reaction for cytomegalovirus DNA G Khan, H O Kangro, P J Coates, R B Heath Cytomegalovirus infection in Gambian mothers and their babies C Bello, H Whittle Serological diagnosis of HIV infection: Practice and performance in western Europe A A Glynn Simplification of procedures used to test urine samples for Chlamydia trachomatis B J Thomas, C Gilchrist, P E Hay, D Taylor-Robinson Identification of Neisseria gonorrhoeae using the Neisstrip rapid enzyme detection test S F Dealler, K R Gough, L Campbell, A Turner, P M Hawkey Suicidal destruction of Helicobacter pylori: Metabolic consequence of intracellular accumulation of ammonia W D Neithercut, M A Greig, M Hossack, K E L McColl Estimation of prevalence of Helicobacter pylori infection in an asymptomatic elderly population comparing ['*C] urea breath test and serology D G Newell, P R Hawtin, A R Stacey, H M MacDougall, A C Ruddle Neutropenia associated with X-linked ag lobuli ia C Kozlowski, D I K Evans Quantitative measurement of faecal blood loss: ‘Comparison of radioisotopic and chemical analyses M B G Leahy, M J Pippard, M B Salzmann, M G Rinsler, R Hesp, T Smith Comparative ferrokinetic study with initial and extended iron clearance models J E Howarth, H M Waters, K Hyde, D Shanks, C G Geary In situ human papillomavirus (HPV) genotyping of cervical intraepithelial neoplasia in South African and British patients: Evidence for putative HPV integration in vivo K Cooper, C S Herrington, A K Graham, M F Evans, J O’D McGee In situ evidence for HPV 16, 18, 33 integration in cervical squamous cell cancer in Britain and South Africa K Cooper, C S Herrington, A K Graham, M F Evans, J O’D McGee Seminal polyamines as agents of cervical carcinoma: Production of aneuploidy in squamous epithelium S Fletcher, W A Neill, M Norval Prognostic value of proliferative activity in lymph node metastases of patients with breast cancer P J van Diest, E Matze-Cok, J P A Baak Epithelial-myoepithelial carcinoma of salivary glands R H W Simpson, T J Clarke, P T L Sarsfield, P G C Gluckman Audit of general surgical pathology experience of histopathology trainees G J Tildsley, S A Dilly Short reports Spinous injury caused by a sea urchin L J McWilliam, A Curry, P L Rowland, J S Watson Evaluation of a 200 mm long vacuum aspiration tube for measurement of erythrocyte sedimentation rate M Casewell, J Stuart Red cell aplasia in myelodysplastic syndrome P J Williamson, D G Oscier, A J Bell, T J Hamblin Undifferentiated carcinoma of parotid gland J / Lopez, J Alfaro, C Ballestin Morbidity survey of post mortem room staff A J Hall, T C Aw, J M Harrington Multipoint microbiological assay for detecting B-lactamase K J Thickett, T G Winstanley Miscellanea Correspondence - Book reviews - Notices - No 6 JUNE 1991 Occasionals Current problems in haematology 2: Hereditary spherocytosis J C Smedley, A J Bellingham ACP Broadsheet 128: Laboratory methods for diagnosing cryptosporidiosis D P Casemore Papers 10 year review of invasive aspergillosis detected at necropsy A P Boon, D O’Brien, D H Adams Toxin production by Bacillus pumilus B Hoult, A F Tuxford Patterns of mortality in sickle cell disease in the United Kingdom A Gray, E N Anionwu, S C Davies, M Brozovic ae sideroblastic anaemia in two girls G Dolan, M M Reid urbidimetric assay for estimating free light chains of immunoglobulins in urine and serum C R Tillyer, J Iqbal, J Raymond, M Gore, T J Mcllwain Parathyroid hormone-related protein of malignancy: I istochemical and biochemical studies in normocalcaemic and hypercalcaemic patients with cancer S H Ralston, J Danks, J Hayman, W D Fraser, C S Stewart, T J Martin Screening for heparin binding variants of antithrombin P L Harper, M Daly, J Price, P F Edgar, R W Carrell Isoenzymes of alkaline phosphatase in epileptic patients receiving carbamazepine monotherapy A B Okesina, D Donaldson, P T Lascelles Routine histological compared with immunohistological examination of bone marrow trephine biopsy specimens in disseminated neuroblastoma M M Reid, J P Wallis, AG McGuckin, A D J Pearson, A J Malcolm Detection of single copies of Epstein-Barr virus in paraffin wax sections by non-radioactive in situ hybridisation P J Coates, W P Mak, G Slavin, A J d’ Ardenne Method for auditing turnround time in histopathology related to user requirements J W M Chow, S A Dilly Analysis of adhesion molecules in the immunopathogenesis of giant cell arteritis SO Wawryk, H Ayberk, A W Boyd, J Rode Knowledge-based computer system to aid in the histopathological diagnosis of breast disease H Heathfield, D Bose, N Kirkham Value of quantitative nucleolar features in the preoperative cytological diagnosis of follicular neoplasias of the thyroid R Montironi, A Braccischi, M Scarpelli,G Matera, R Alberti Short reports Detection and identification of Helicobacter pylori by the polymerase chain reaction C Clayton, K Kleanthous, S Tabagchali Helicobacter pylori and ABO blood groups R J L F Loffeld, E Stobberingh Anticardiolipin antibodies in leptospirosis F P Rugman, G Pinn, M F Palmer, M Waite, C R M Hay Cachexia and tumour necrosis factor-a in cytomegalovirus infection H Tilg, W Vogel, M Herold, W E Aulitzky, C Huber Rapid identification of Ebola virus and related filoviruses in fluid specimens using indirect immunoelectron microscopy T W Geisbert, J B Rhoderick, P B Jahrling Exaggerated and prolonged thyrotrophin releasing hormone (TRH) test responses in tertiary hypothyroidism G H Mills, R D Ellis, P R Beck Miscellanea Correspondence - Book reviews - Notices - No 7 JULY 1991 Occasionals ACP Presidential address: Screening for presymptomatic disease F V Flynn Use of information technology for auditing effective use of laboratory services M Peters, P M G Broughton, P G Nightingale Troublesome tumours 1: Adnexal tumours of the skin D Cotton Papers Expression of intrahepatic hepatitis D viral antigen in chronic hepatitis D virus infection / Y N Lau, L J Hansen, V G Bain, et al Detection of hepatitis B virus pre-S1 and pre-S2 determinants in paraffin wax embedded liver tissue: Importance of reagents used N Hadzic, A Alberti, B Portmann, D Vergani Histological diagnosis of intestinal microsporidiosis in patients with AIDS C S Peacock, C Blanshard, D G Tovey, D S Ellis, B G Gazzard Detection of Chlamydia trachomatis DNA in patients with non-gonococcal urethritis using the polymerase chain reaction G Ratti, A Moroni, R Cevenini Comparison of IgG antibody profiles by immunoblotting in patients with acute and previous Toxoplasma gondii infection J L Moir, M M Davidson, D O Ho-Yen Prealbumin in the diagnosis of bronchopulmonary carcinoid tumours U R Suresh, S Wilkes, P S Hasleton Endocardial fibroelastosis in infants with hydrops fetalis M J Newbould,G R Armstrong, A J Barson Expression of neural cell adhesion molecule (NCAM) isoforms in neuroblastoma E Phimister, F Kiely, J T Kemshead, K Patel Vessel associated adhesion molecules in normal skin and acute graft-versus-host disease J Norton, J P Sloane, N Al-Saffar, D O Haskard Microbiological assay for vitamin B12 performed in 96-well microtitre plates B P Kelleher, S D O Broin Serum C-reactive protein and neopterin concentrations in patients with viral or bacterial infection A C Shaw Role of mesothelial cells in peritoneal antibacterial defence M A Muijsken, H J C M Heezius, J Verhoef, H A Verbrugh Short reports Extracting, storing, and transporting whole blood DNA under tropical conditions J] Bates, G Bedu-Addo, T R Rutherford Use of leucocyte alkaline phosphatase (LAP) score in differentiating malignant from benign paraproteinaemias G Majumdar, M Hunt, A K Singh Enterococcal meningitis in an HIV positive haemophilic patient W N Patton, N Bienz, I M Franklin, J G M Hastings Haemorrhagic colitis and haemolytic-uraemic syndrome: False positive reaction with a rotavirus latex agglutination test R P Bendall, J J Gray Detection of Borrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction E C Guy, G Stanek Helicobacter pylori and gastric histamine concentrations D M M Queiroz, E N Mendes, G A Rocha, et al Miscellanea Correspondence - Book reviews - Notices - No 8 AUGUST 1991 Occasionals WELCAN UK: Its development and future C H N Bennett Early clinical pathologists 4: John Hunter (1728-1793) S Lakhani ACP Broadsheet 129: Isolation and identification of Listeria monocytogenes K G Kerr, R W Lacey Papers Heterogeneity of T cell lymphoblastic leukaemias E Gomez, J F San Miguel, M Gonzalez, et al Immunohistological diagnosis of “plasmacytoid T cell lymphoma” in paraffin wax sections J O Thomas, K Beiske, I Hann, C Koo, D Y Mason Ber-ACTS8: New monoclonal antibody to the mucosa lymphocyte antigen M Kruschwitz, G Fritzsche, R Schwarting, et al Basement membrane proteins in the space of Disse: a reappraisal M R Griffiths, S Keir, A D Burt Breslow thickness of cutaneous malignant melanoma in paraffin wax and frozen sections N Kirkham, K Blessing, B Gibson, M L Price Middle ear adenoma: Tumour of mixed mucinous and neuroendocrine differentiation R J Hale, R F T McMahon, J S Whittaker Prognostic value of proliferating cell nuclear antigen in gastric carcinoma S Jain, M I Filipe, P A Hall, N Waseem, DP Lane, D A Levison Comparison of commercially available cytokeratin antibodies in normal and neoplastic adult epithelial and non- epithelial tissues M J Goddard, B Wilson, J W Grant Use of monoclonal antibodies to recognise osteoclasts in routinely processed bone biopsy specimens N A Athanasou,B Puddle, J-Quinn, C G Woods Infections in British clinical laboratories, 1988-1989 N R Grist, J A N Emslie Streptococci from primary isolation plates grouped by reverse passive haemagglutination M Farrington, S Winters, D Rubenstein, J Greatorex, R Whetstone Detection of Epstein-Barr virus genomes in AIDS related lymphomas: Sensitivity and specificity of in situ hybridisation compared with Southern blotting S J Hamilton-Dutoit, H J Delecluse, M Raphael, G Lenoir, G Pallesen Nasal, axillary, and perineal carriage of Staphylococcus aureus among women: Identification of strains producing epidermolytic toxin S J Dancer, W C Noble Comparison of assay systems for detecting antibodies to nuclear ribonucleoproteins P Merryman, P Louie Short reports New sternal puncture needle A Islam HTLV-1 associated T cell lymphoma in South East Asia: Case report and family study T L Wang, T C Liu, L Chan, Y K Kueh, S Y Wong Treatment of acute myeloid leukaemia in a renal allograft recipient: Implications of cyclosporin immunosuppressive treatment R J G Cuthbert, N H Russell, P A E Jones, A G Morgan Inhibition of urease activity but not growth of Helicobacter pylori by acetohydroxamic acid J Goldie, S J O Veldhuyzen van Zanten, S Jalali, H Richardson, R H Hunt Detection of Helicobacter pylori carriers by discriminant analysis of urea and pH levels in gastric juices F Ameglio, M R Abbolito, D Giannarelli, et al Acanthamoeba keratitis: Experience in a non-specialist microbiology laboratory D Anderson, S S Soo, H Towler Miscellanea Correspondence - Book reviews - Notices No 9 SEPTEMBER 1991 Occasionals Costing of pathology services in the United Kingdom National Health Service H M Dick Pathology reporting in breast cancer screening Royal College of Pathologists Working Group Pathological mimics of chronic inflammatory bowel disease N A Shepherd Papers Blood component treaiment: a retrospective audit in five major London hospitals A Thomson, M Contreras, S Knowles Antibiotic treatment and associated prolonged prothrombin time K J Williams, R P Bax, H Brown, S J Machin Diamond-Blackfan syndrome and neutropenia K P Schofield, D I K Evans Colour perception in pathologists: the Farnsworth-Munsell 100-hue test H S Rigby, B F Warren, J Diamond, C Carter, J W B Bradfield Video absorbtiometry in diagnostic cytology: Description of a new technique and a preliminary statistical evaluation J A Lee, D J Spargo, P A Riley Immunoglobulin heavy chain patterns in reactive lymphadenopathy M Sundaresan, T Rhodes, A B Akosa Silver enhancement of polymerised diaminobenzidine: Increased sensitivity for immunoperoxidase staining C S Peacock, I W Thompson, S Van Noorden Use of a tissue adhesive to repair fetal bodies after dissection G S Gau, K Napier, J Bhundia Selective location of palisaded myofibroblastoma with amianthoid fibres G Bigotti, A Coli, M Mottolese, F Di Filippo Simple and specific test for measuring lipid peroxides in plasma P Gérég, D C Kotak, I B Kovacs Immune responses to Helicobacter pylori in children with recurrent abdominal pain J E Crabtree, M J Mahony, J D Taylor, R V Heatley, J M Littlewood, D S Tompkins Problems of thermonuclease detection for identifying Staphylococcus aureus in blood culture broths G M Megson, D Law, L A Ganguli Evaluation of the Microring YT system for identifying clinical yeast isolates E J Ridgway, K D Allen Comparison of cell culture with an amplified enzyme immunoassay for diagnosing genital herpes simplex infection G Kudesia, A Van Hegan, S Wake, R J Van Hegan, G R Kinghorn Short reports A tumour, a cell, a misunderstanding: Trabecular (Merkel cell) carcinoma of the skin C Urso Should eye protection be worn when performing necropsies? A D Bull, J Channer, S S Cross, R D Start, A Kennedy Cerebrospinal fluid concentration of fibronectin in meningitis D Torre, C Zeroli, M Isst, G P Fiori, G Ferraro, F Speranza Assessment of the Glycotronic C blood glucose meter A R W Forrest, E J Pitt Sudden postoperative death caused by unheralded Mallory Weiss tears G M E Humphrey, E W Benbow, W F Tait Platelet satellitism and a granule proteins C Christopoulos, C Mattock Miscellanea Correspondence - Book reviews - Notices - Correction No 10 OCTOBER 1991 Occasionals Good laboratory management: an Anglo-American perspective J Stuart, J M Hicks Pathology department accreditation in the United Kingdom: a synopsis Advisory Task Force on Standards to the Audit Steering Committee of the Royal College of Pathologists The pathology of heart and heart and lung transplantation—an update S Stewart, N Cary Papers Beta,-glycoprotein-1 (apolipoprotein H) excretion in chronic renal tubular disorders: Comparison with other protein markers of tubular malfunction M Lapsley, P A Sansom, C T Marlow, F V Flynn, A G W Norden Intestinal origin alkaline phosphatase activity in plasma for differential diagnosis of jaundice T Kuwana, S B Rosalki Disseminated Pneumocystis carinii infection in AIDS R J Coker, D Clark, E L Claydon, et al B-D-Glucuronidase activity assay for rapid differentiation of species within B-haemolytic group C and G streptococci N Cimolai, D Mah Vaginopathic and proteolytic Candida species in outpatients attending a gynaecology clinic L Agatensi, F Franchi, F Mondello, et al Evaluation of commercial enzyme linked immunosorbent assay for detection of B19 parvovirus IgM and IgG G Patou, U Ayliffe Model system for optimising mRNA non-isotopic in situ hybridisation: Riboprobe detection of lysozyme mRNA in archival gut biopsy specimens J C Martinez-Montero, C S Herrington, J Stickland, et al Oestrogen receptors in conjunctival malignant melanoma: I ytochemical study using formalin fixed paraffin wax sections D A Paridaens, R A Alexander, J L Hungerford, A C E McCartney Detection of Epstein-Barr virus genomes in Hodgkin’s disease: Relation to age R F Jarrett, A Gallagher, D B Jones, etal Quantitative Limulus lysate assay for endotoxin and the effect of plasma J C Hurley, F A Tosolini, W J Louis Alpha,-antitrypsin deficiency, complement activation, and chronic liver disease E T Littleton, L Bevis, L J Hansen, et al How common is cholesterol embolism? S S Cross Audit of necropsies in a British district general hospital M D Harris, J W Blundell Short reports Intestinal graft versus host disease R L Bryan, G N A k los, J Ni ,D W Milligan Malignant glioma in laboratory workers G N Rutty, M Honavar, B Doshi Familial antithrombin III deficiency and Mycoplasma pneumoniae pneumonia M D Creagh, I F Roberts, D J Clark, F E Preston No evidence of HTLV-I infection in French patients with multiple sclerosis using the polymerase chain reaction E Wattel, M Mariotti, J D Bignon, et al Staphylococcus lugdunensis K F Barker, J C O’Driscoll, A Bhargava Marking of resection margins C Hunter-Craig, B Lee-McDonagh, H G Penman Miscellanea Correspondence - Book reviews - Notices No 11 NOVEMBER 1991 Occasionals Current concerns in haematology 3: Blood count calibration S M Lewis, J M England, R M Rowan Guidelines on testing for the lupus anticoagulant Lupus Anticoagulant Working Party on behalf of the BCSH Haemostasis and Thrombosis Task Force ACP Broadsheet 130: Gross examination of bladder specimens M C Parkinson, C Fisher Papers Endocervical carcinoma and precursor lesions: c-myc expression and the demonstration of field changes S V Polacarz, J Darne, E G Sheridan, R Ginsberg, F Sharp Feasibility of in situ hybridisation with chromosome specific DNA probes on paraffin wax embedded tissue E J P Arnoldus, E J Dreef,I A Noordermeer, et al Rapid detection of hepatitis B virus DNA in liver tissue by in situ hybridisation and its combination with .: xchemistry for simultaneous detection of HBV antigens J Y N Lau, N V Naoumov, G J M Alexander, R Williams Central pontine myelinolysis in liver transplantation A P Boon, M P Carey, D H Adams, J Buckels, PM cMaster T cell receptor VB expression by mucosal T cells J Spencer, M Y Choy, T T MacDonald Early delayed hypersensitivity responses in tuberculin skin tests after heavy occupational exposure to tuberculosis J H Gibbs, J M Grange, J Swanson Beck, et al Removal of inhibitor(s) of the polymerase chain reaction from formalin fixed, paraffin wax embedded tissues S F An, K A Fleming Prospective study of quantitative aspects of audit in a large general histopathology laboratory J St J Thomas, AM Lessells, M A McIntyre, H S Klys, J N Webb Western blot analysis of immune response to Legionella bozemanii antigens J Boldur, H Kahana, R Kazak, B Cwikel, I Sarov Ber-MAC3: New monoclonal antibody that defines human monocyte/macrophage differentiation antigen E Backé, R Schwarting, J Gerdes, M Ernst, H Stein Assessment of Diesse Ves-matic automated system for measuring erythrocyte sedimentation rate M Caswell, J Stuart Evaluaton of the Ciba Corning Biotrack 512 coagulation monitor for the control of oral anticoagulation J Jennings, R J Luddington, T Baglin Short reports Bone marrow aplasia in B cell chronic lymphocytic leukaemia: Successful treatment with antithymocyte globulin R Singal, D A Winfield, M Greaves Bleeding diathesis coincident with chronic myelomonocytic leukaemia S E Heard, P Revell, L J Holland, AD F Hurdle, G F Savidge Squamous cell carcinoma arising in endometriosis of the ovary K N Naresh, V K Ahuja, C Rama Rao, G Mukherjee, M K Bhargava Lack of c-erbB-2 oncoprotein expression in male breast carcinoma S B Fox, C A Day, S Rogers Tuberous sclerosis and parathyroid adenoma L S Mortensen, J Rungby Distinction between antinuclear antibody and P-ANCA S S Lee, J W M Lawton, W Chak Miscellanea Correspondence - Book reviews - Notices No 12 DECEMBER 1991 Occasionals Current views on cervical intraepithelial neoplasia M C Anderson, C L Brown, C H Buckley, et al Wiskott-Aldrich syndrome: a multidisciplinary disease G R Standen Papers Quantitative interrelations of Lewis antigens in normal mucosa and transitional cell bladder carcinomas C Limas Episomal and integrated human papillomavirus in cervical neoplasia shown by non-isotopic in situ hybridisation K Cooper, C S Herrington, J E Strickland, M F Evans J O’D McGee Cytological features of non-small cell carcinomas of the lung in fine needle aspirates S B Zusman-Harach, H R Harach, R Gibbs Lung mast cells in plexogenic pulmonary arteriopathy D Heath, M Yacoub New automated technique for assessing emphysema on histological sections M Gillooly, D Lamb, A S J Farrow Tissue distribution of human T cells: No evidence for general epithelial tropism TM Vroom, G Scholte, F Ossendorp, J Borst Mnemonic coding system for clinical data entry into laboratory computers: Its effect on quality and efficiency IR Bailey, K B Page, R G Jones, R B Payne, A J Little Molecular specificity of two commercial enzyme linked immunosorbent assays for human immunodeficiency virus antigens K E Brown, D C Shanson, P Mascagni, A R M Coates Short reports Rapid screening for toxigenic Corynebacterium diphtheriae by the polymerase chain reaction M J Pallen Demonstration of Chlamydia trachomatis in colposcopic cervical biopsy specimens by an immunoperoxidase method K Edwards, A R Campbell, A Tait, M Lusher Evaluation of a screening test for detecting urinary tract infection in newborns and infants B Lejeune, R Baron, B Guillois, D Mayeux Oncocytic metaplasia of the nasopharynx or extra-parotid Warthin’s tumour? A P Griffiths, P Dekker Atypical Ph negative chronic myeloid leukaemia presenting as sudden profound deafness N Smith, B Bain, L Michaels, E Craven Pulmonary infarction, myocardial infarction, and acute disseminated intravascular coagulation F J Thomson, E W Benbow, R F T McMahon, C M Cheshire Miscellanea Correspondence - Book reviews - Notices List of assessors Annual author index to Vol 44 Annual subject index to Vol 44 Annual contents index to Vol 44