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NEWS In This Issue B • Loopy Sec61 mutants • MITF links maturation and quiescence • p 107 Skps from S phase • Thinning microtubules • Freedom in condensation THE JOURNAL OF CELL BIOLOGY N. LeBrasseur f' Research Roundup VOL. 188, NO. 1, JANUARY 3, 2005 1 O • Lengthening without letting go • The oenocytes went 3 by 3 5 • Exclusion is spreading • Stretch but don’t follow • One more C means no hearing W.A. Wells From The Archive 12 •A new take on the old • The discovery of synaptic vesicles • Ribosomes, or the particles of Palade • Microsomes are the in vitro ER W.A. Wells- - years of cell biology Editorials 1 5 Fifty years of cell biology I. Mellman On the coven This issue marks the 50th anniversary of the Journal of Cell Biology. The cover incorporates just o handful of the amazing REVIEWS images published in these pages over the Mini-Reviews past 50 years. Cover design by Man-Wai Cheung and Mina Muto, Adolescent, Inc., 1 "7 Hydrogen peroxide as a signal controlling plant programmed New York, NY (www.adolescent.tv). cell death T.S. Gechev andj. Hille RESEARCH ARTICLES Reports 2 1 Centrosome fragments and microtubules are transported asymm.etrically away from division plane in anaphase N.M. Rusan and P. Wadsworth 29 Glycogen synthase kinase-3 is an endogenous inhibitor of Snail transcription; implications for the epithelial-mesenchymal transition R.E. Bachelder, S.O. Yoon, C. Franci, A. Garcia de Herreros, and A.M. AAercurio 35 MITF links differentiation with cell cycle arrest in melanocytes by transcriptional activation of 1NK4A A.E. Loercher, E.M.H. Tank, R.B. Delston, andJ.W. Harbour Articles 4 1 Condensed mitotic chromatin is accessible to transcription factors and chromatin structural proteins D. Chen, M. Dundr, C. Wang, A. Leung, A. Lomond, T. Misteli, and S. Huang Articles with related stories in the IN THIS ISSUE section have page numbers in RED.. 5S pi07 inhibits G1 to S phase progression by down-regulating expression of the F-box protein Skp2 G. Rodier, C. Makris, P. Coulombe, A. Scime, K. Nakayama, K.l. Nakayama, and S .Mebche B7 Identification of cytoplasmic residues of Sec61p involved in ribosome binding and cotranslational translocation Z. Cheng, Y. Jiang, E.C. Mandon, and R. Gilmore 73 Specialized membrane-localized chaperones prevent aggregation of polytopic proteins in the ER J. Kota and P.O. Ljungdahl 85 HECT ubiquitin ligases link viral and cellular PPXY motifs to the vacuolar protein-sorting pathway J. Martin-Serrano, S.W. Eastman, W. Chung, and P.D. Bieniasz 103 Mdm31 and Mdm32 are inner membrane proteins required for maintenance of mitochondrial shape and stability of mitochondrial DNA nucleoids in yeast K. S. Dimmer, S. Jakobs, F. Vogel, K. Altmann, and B. Westermann 1 1 T The PSD95-nNOS interface; a target for inhibition of excitotoxic p38 stress-activated protein kinase activation and cell death A HECT ubiquitin ligase (green) brings J. Cao, J.l. Vihobinen, C. Dart, H.K. Warwick, M.L. Leybnd, and MJ. Courtney the Ebola virus (red) to the VPS pathway during virus budding. 1 37 RPTPa is essential for NCAM-mediated p59''" activation and See page 8S. neurite elongation V. Bodrikov, I. Leshchyns'ka, V. Sytnyk, J. Overvoorde, J. den Hertog, and AA. Schachner T 41 CLASP 1 and CLASP2 bind to EBl and regulate microtubule plus-end dynamics at the cell cortex Y. Mimori-Kiyosue, I. Grigoriev, G. Lansbergen, H. Sasaki, C. Matsui, F. Severin, N. Galjart, F. Grosveld, I. Vorobiev, S. Tsukita, and A. Akhmanova 1 55 Cortactin and dynamin are required for the clathrin-independent endocytosis of yc cytokine receptor N. Sauvonnet, A. Dujeancourt, and A. Dautry-Varsat NCAM (red) activates a Src family kinase via the RPTPa phosphatase (green). See page 1S7. NEWS In This Issue 1 Myo6 gets choosy A GTP signal to the nucleolus Growth and trafficking Actin-myosin tears it up Modeling distinct compartments R.S. Tuma From The Archive 1 “7 <4 • Catching sight of lysosomes • The invention of freeze fracture EM and the determination of membrane structure W.A. Wells REVIEWS Comments '17 7 Going in GTP cycles in the nucleolus T. Misteli RESEARCH ARTICLES Reports ITS A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin R.Y.L. Tsai and Ronald D.G. McKay 1 B 5 Cell growth-dependent coordination of lipid signaling and glycosylation is mediated by interactions between Sac Ip and Dpmlp F. Faulhammer, G. Konrad, B. BrankatscFik, S. Tahirovic, A. Knodler, and P. Mayinger 133 Muscle expression of a local Igf-l isoform protects motor neurons in an ALS mouse model On the cover G. Dobrowolny, C. Giacinti, L. Pelosi, C. Nicolefti, N. Winn, L. Barberi, M. Molinaro, N. Rosenthal, and A. Musaro Inhibition of actomyosin contraction protects nuclear membrane integrity in apoptotic 201 The offloading model for dynein function: differential function cells, although condensed chromosomes of motor subunits can be seen at the nuclear periphery. W.-L. Lee, M.A. Kaiser, andJ.A. Cooper See page S45. Articles 203 Spindle-independent condensation-mediated segregation of yeast ribosomal DNA in late anaphase F. Machin,J. Torres-Rosell, A. Jarmuz, and L. Aragon 221 Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis F. Oceguera-Yanez, K. Kimura, S. Yasuda, C. Higashida, T. Kitomura, Y. Hiraoka, T. FHaraguchi, and S. Narumiya 233 Nuclear transport of single molecules: dwell times at the nuclear pore complex U. Kubitscheck, D. Griinwald, A. Hoeksfta, D. Rohleder, T. Kues, J.P. Siebrasse, and R. Peters Articles with related stories in the IN THIS ISSUE section have page numbers in RED; articles with COMMENTS have page numbers in BLUE. S 4 5 Actin-myosin-based contraction is responsible for apoptotic nuclear disintegration D. R. Croft, hA.l. Coleman, S. Li, D. Robertson, T. Sullivan, C.L. Stewart, and M.F. Olson S57 Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast A.I. Pozniakovsky, D.A. Knorre, O.V. Markova, A.A. Hyman, V.P. Skulachev, and F.F. Severin 2 7 i Generation of nonidentical compartments in vesicular transport systems R. HeinricFi and T.A. Rapoport as 1 ARFGAPl plays a central role in coupling COPl cargo sorting with vesicle formation S. Y. Lee, J.-S. Yang, W. Hong, R.T. Premont, and V.W. Hsu a 31 BACE overexpression alters the subcellular processing of APP and inhibits AP deposition in vivo E. B. Lee, B. Zhang, K. Liu, E.A. Greenbaum, R.W. Dorns, J.Q. Trojanowski, and V.M.-Y. Lee Lumen formation in epithelial cell cysts is 303 Gpl35/podocalyxin and NHERF-2 participate in the formation abnormal in the absence of podocalyxin, of a preapical domain during polarization of MDCK cells an apical protein that helps polarize the cells before cell junctions form. D. Meder, A. Shevchenko, K. Simons, andj. Fullekrug See page 303. 315 Effects of Arp2 and Arp3 nucleotide-binding pocket mutations on Arp2/3 complex function A.C. Martin, X.-P. Xu, I. Rouiller, M. Kaksonen, Y. Sun, L. Belmont, N. Volkmonn, D. Hanein, M. Welch, and D.G. Drubin 3 23 A role for myosin VI in postsynaptic structure and glutamate receptor endocytosis E. Osterweil, D.G. Wells, and M.S. Mooseker NEWS In This Issue 346 • No vacuolar reunion BiP chaperones ER entry Yeast warfare Neurons born in new places Ring around the wound N. LeBrasseur Research Roundup VOL. 1.68, NO. 3, JANUARY 31, 2005 348 • Saving varicella from suicide • Thinner tubes • Pore before seal • Kinetochores hold on with a ring • Development is easy W.A. Wells From The Archive 350 • Cilia get arms for bending W.A. Wells ' ’ RESEARCH ARTICLES Reports 353 Viral killer toxins induce caspase-mediated apoptosis in yeast • ' - . -• r J. Reiter, E. Herker, F. Madeo, and M.J. Schmitt 359 Myosin V attachment to cargo requires the tight association of two functional subdomains N. Pashkova, N.L. Catlett, J.L. Novak, G. Wu, R. Lu, R.E. Cohen, and L.S. Weisman Articles 365 X chromosome choice occurs independently of asynchronous replication timing J. Gribnau, S. Luikenhuis, K. Hochedlinger, K. Monkhorst, and R.Jaenisch On the cover 375 Chromosome looping in yeast: telomere pairing and coordinated A ring of active Cdc42 (red) around a movement reflect anchoring efficiency and territorial organization ring of active RhoA (green) pulls in the K. Bystricky, T. Laroche, G. van Houwe, M. Blaszczyk, and S.M. Gasser actin network to cover the membrane at a wound site. 389 The molecular mechanisms underlying BiP-mediated gating of the See page 4SS. Sec61 translocon of the endoplasmic reticulum N.N. Alder, Y. Shen, J.L. Brodsky, L.M. Hendershot, and A.E. Johnson 401 The vacuolar kinase Yck3 maintains organelle fragmentation by regulating the HOPS tethering complex TJ. LaGrass.3 and C. Ungermann 415 New GABAergic interneurons in the adult neocortex and striatum are generated from different precursors A.G. Dayer, K.M. Cleaver, T. Abouantoun, and H.A. Cameron 4 S 9 Concentric zones of active RhoA and Cdc42 around single cell wounds FH.A. Benink and W.M, Bement Articles with related stories in the IN THIS ISSUE section have page numbers in RED. Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cotilin H. Yamaguchi, M. Lorenz, S. Kempiak, C. Sarmiento, S, Coniglio, M. Symons, J. Segoll, R. Eddy, H. Miki, T. Tokenowo, ondj. Condeelis 453 Receptor tyrosine phosphatase-dependent cytoskeletal remodeling by the hedgehog-responsive gene MIM/BEG4 R. Gonzolez-Quevedo, AA. Shoffer, L. Horng, and A.E. Oro 465 Ultrastructural identification of uncoated caveolin-independent early endocytic vehicles M. Kirkhom, A. Fujito, R. Choddo, SJ. Nixon, T.V. Kurzcholio, D.K. Shormo, R.E. Pogono, J.F. Hancock, S. Mayor, and R.G. Parton 477 Clathrin- and caveolin-1-independent endocytosis: entry of simian virus 40 into cells devoid of caveolae E.-M. Damm, L. Pelkmans,J. Kartenbeck, A. Mezzacasa, T. Kurzchalia, and A. Helenius 465 Catalytically inactive human cathepsin D triggers fibroblast invasive growth V. Laurent-Mafha, S. Maruani-Herrmann, C. Prebois, M. Beaujouin, M. Glondu, A. Noel, M..L. Alvarez-Gonzalez, S. Blacher, P, Coopman, S. Baghdiguian, C. Gilles, J. Loncarek, G. Freiss, F. Vignon, and E. Liaudef-Coopman 501 Regulation of aSpi integrin conformation and function by MIM (green) remodels the cytoskelet urokinase receptor binding by bundling F-actin (red) near focal Y. Wei, R.-P. Czekay, L. Robillard, M.C. Kugler, F. Zhang, K.K. Kim,J.-p. Xiong, adhesions (purple). M.J. Humphries, and H.A. Chapman See page 453. NEWS In This Issue 5SO • Lamellae lead the way • A stalled fork stalls recombination Another job for P-catenin Working up to the last minute Severing saves microtubules R.S. Tuma Research Roundup 5SS • Blood pressure and fertility • Calcium says stop • Ribosome does it all • Lumen cues calcium release • Supersensitive phospholipid sensing N. LeBrasseur From The Archive 5S4 • Heterochromatin is late • The nucleolar origin of rRNA • A pathway for secretion W.A. Wells Meeting Report 5S6 • Cells in D.C.: The American Society for Cell Biology Washington, D.C. December 4-8, 2004 N. LeBrasseur and K. Powell REVIEWS Mini-Reviews S33 Cellular organization by self-organization: mechanisms and models for Min protein dynamics M. Howard and K. Kruse RESEARCH ARTICLES On the coven Reports Microtubules (green) are severed by spastin (red). This severing activity is needed for 537 Temporal separation of replication and recombination requires axon survival. the intra-S checkpoint See page 5SS. P. Meisfer, A. Taddei, L. Vernis, M. Poidevin, S.M. Gasser, and G. Baldacci 545 Protein synthesis persists during necrotic cell death X. Saelens, N. Festjens, E. Parthoens, I. Vanoverberghe, M. Kalai, F, van Kuppeveld, and P. Vandenabeele 553 Reduction of total E2F/DP activity induces senescence-like cell cycle arrest in cancer cells lacking functional pRB and p53 K. Maehara, K. Yamakoshi, N. Ohtani, Y. Kubo, A. Takahashi, S. Arase, N. Jones, and E. Hara 561 CDK4 regulation by TNFRI and JNK is required for NF-KB-mediated epidermal growth control J.Y. Zhang, S. Tao, R. Kimmel, and P.A. Khavari 567 Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration B. Stramer, W. Wood, M.j. Gaiko, MJ. Redd, A. Jacinto, S.M. Parkhurst, and P. Martin Articles with related stories in the IN THIS ISSUE section have page numbers in RED. Articles S7S DRhoGEFl regulates actin organization and contractility in the Drosophila blastoderm embryo M. Padash Barmchi, S. Rogers, and U. Hacker 5B7 Structural determinants for EBI-mediated recruitment of APC and spectraplakins to the microtubule plus end K. C. Slep, S.L. Rogers, S.L. Elliott, H. Ohkuro, P.A. Kolodziej, and R.D. Vole SSS Linking axonal degeneration to microtubule remodeling by Spastin-mediated microtubule severing KJ. Evans, E.R. Gomes, S.M. Reisenweber, G.G. Gundersen, and B.P. Lauring 607 Botulinum neurotoxin C initiates two different programs for neurite degeneration and neuronal apoptosis L. Berliocchi, E. Fava, M. Leist, V. Horvat, D. Dinsdale, D. Read, and P. Nicotera * 6 19 Cell migration without a lamellipodium: translation of actin •* ■ #• » • \ ** - dynamics into cell movement mediated by tropomyosin ‘ • •• ♦A , • ' S.L. Gupton, K.L. Anderson, T.P. Kole, R.S. Fischer, A. Ponti, '-Af: S.E. Hitchcock-DeGregori, G. Danuser, V.M. Fowler, D. Wirtz, D. Hanein, 4 and C.M. Waterman-Storer 633 LI, a novel target of 3-catenin signaling, transforms cells and is expressed at the invasive front of colon cancers N. Gavert, M. Conacci-Sorrell, D. Gast, A. Schneider, P. Alfevogt, T. Brabletz, and A. Ben-Ze'ev 643 Inhibition of endothelial cell migration by thrombospondin-1 The structure of the COOH-terminal domain type-1 repeats is mediated by integrins of EBl reveals how this microtubule plus- S.M. Short, A. Derrien, R.P. Narsimhan,J. Lawler, D.E. Ingber, and B.R. Zetter end binding protein binds to regulators of cell polarity. 655 Coordinate control of axon defasciculation and myelination by See page 587. laminin-2 and -8 D. Yang,J. Bierman, Y.S. Tarumi, Y.-P. Zhong, R. Rangwala, T.M. Proctor, Y. Miyagoe-Suzuki, S. Takeda,J.H. Miner, L.S. Sherman, B.G. Gold, and B.L. Patton NEWS In This Issue B7S • Cooperative kinesin • Crossovers break SC symmetry • Myristoylation versus SRP • Bringing PIPs to root tips • Nerve damage surveillance THE JOURNAL OF CELL BIOLOGY N. LeBrasseur Research Roundup B74 • Bugs get decrepit too • Chemotaxis by local steering • No exit for Ca""^ • Diet affects DNA • Bacteria keep oxygen flowing W.A. Wells From The Archive B7B • How to spot a satellite cell • Autophagy unveiled • How vessels become leaky W.A. Wells REVIEWS Mini-Reviews B "7 9 Conservation of boundary extension mechanisms between plants and animals J. Mathur t. ^0 RESEARCH ARTICLES Reports B 8 3 Crossing over is coupled to late meiotic prophase bivalent On the cover differentiation through asymmetric disassembly of the SC K. Nabeshima, A.M. Villeneuve, and ^A.P. Colaidcovo During meiotic prophase, SC proteins (green) leave chromosomes (red) B9 1 Long-range cooperative binding of kinesin to a microtubule asymmetrically after recombination. in the presence of ATP See page G83. E. Muto, H. Sakai and K. Kaseda B 9 7 Role of cytoplasmic dynein in the axonal transport of microtubules and neurofi laments Y. He, F. Francis, K.A. Myers, W. Yu, M.M. Black, and P.W. Baas 705 PATJ regulates tight junction formation and polarity in mammalian epithelial cells K. Shin, S. Straight, and B. Margolis Articles 7 T 3 The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation S. La Terra, C.N. English, P. Hergert, B.F. McEwen, G. Sluder, and A. Khodjakov 793 Bc1-Xl specifically activates Bak to induce swelling and restructuring of the endoplasmic reticulum M. Klee and F.X. Pimentel-Muinos Articles with related stories in the IN THIS ISSUE section have page numbers in RED. •735 N-myristoylation determines dual targeting of mammalian NADH-cytochrome b(5) reductase to ER and mitochondrial outer membranes by a mechanism of kinetic partitioning S. Colombo, R. Longhi, S. Alcoro, F. Ortuso, T. Sprocoti, A, Flora, and N. Borgese ■74-7 Cog3p depletion blocks vesicle-mediated Golgi retrograde trafficking in HeLa cells S. N. Zolov and V.V. Luposhin Dynamic ergosterol- and ceramide-rich domains in the peroxisomal membrane serve as an organizing platform for peroxisome fusion T. Boukh-Viner, T. Guo, A. Alexandrian, A. Cerrocchio, C. Gregg, S. Haile, R. Kyskon, S. Milijevic, D. Oren,J. Solomon, V. Wong,J,-M. Nicoud, R. A. Rachubinski, A.M. English, and V.l. Titorenko ■7 75 Sunday Driver links axonal transport to damage signaling V. Cavalli, P. Kujala,]. Klumperman, and L.S.B. Goldstein 7S9 Regulation of the interaction between PlPKly and talin by proline-directed protein kinases S. Y. Lee, S. Voronov, K. Lefinic, A.C. Nairn, G. Di Paolo, and P. De Camilli 501 A Secl4p-nodulin domain phosphatidylinositol transfer protein Cancer cells that have lost their centrioles polarizes membrane growth of Arabidopsis thaliana root hairs assemble new ones (arrow) de novo and thus do not arrest in Gi. P. Vincent, M. Chua, F. Nogue, A. Fairbrother, H. Mekeel, Y. Xu, N. Allen, See page 713. T. N. Bibikova, S. Gilroy, and V.A. Bankaitis 513 The LIM protein Ajuba influences pl30Cas localization and Racl activity during cell migration S.J. Pratt, H. Epple, M. Ward, Y. Feng, V.M. Braga, and G.D. Longmore BS5 Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex S. tom Dieck, W.D, Altrock, M.M. Kessels, B. Qualmonn, H. Regus, D. Brauner, A. Fejtovd, O. Bracko, E.D. Gundelfinger, andJ.H. Brandstdtter