JOURNAL OF ASSISTED REPRODUCTION AND GENETICS CONTENTS Volume 17, Number | January 2000 CLINICAL ASSISTED REPRODUCTION Human Embryo Development and Pregnancies in an Homologous Granulosa Cell Coculture System Raffaella Fabbri, Eleonora Porcu, Tiziana Marsella, Maria Rita Primavera, Sandra Cecconi, Stefania A. Nottola, Pietro M. Motta, Stefano Venturoli, and Carlo Flamigni Human gri osa ce Cc uilures had ) noryo evelopment (but not on pregnancy rates) in patients in the first cycle of IVF-ET Ovarian Reserve Test with Human Menopausal Gonadotropin as a Predictor of In Vitro Fertilization Outcome Francisco Fabregues, Juan Balasch, Montserrat Creus, Francisco Carmona, Bienvenido Puerto, Lloreng Quinté, Roser Casamitjana, and Juan A, Vanrell rhe ovarian reserve test with hMG predic ‘ response in IVF than the woman’s age and hormonal nul age Follicular Development and Hormonal Levels Following Highly Purified or Recombinant Follicle- Stimulating Hormone Administration in Ovulatory Women Undergoing Ovarian Stimulation After Pituitary Suppression for In Vitro Fertilization: Implications for Implantation Potential Juan Balasch, Francisco Fdabregues, Montserrat Creus, Joana Penarrubia, Esther Vidal, Francisco Carmona, Bienvenido Puerto, and Juan A. Vanrell Recombinant FSH is more efficacious \ fied iry FSH when used in IVI The Effects of E, Supplementation from the Early Proliferative Phase to the Late Secretory Phase of the Endometrium in hMG-Stimulated IVF-ET Hyuk Jung and Hyoung Kyun Roh I supppplel ementation from the ry phase of the endometrium in hMG-stimulated [VF-ET f the 1 clinical pregnancy rates Is the Timing of Implantation Affected by Zona Pellucida Micromanipulation? Ming-I Hsu, Gerardo Barroso, Jacob Mayer, Susan Lanzendorf, William E. Gibbons, Suheil Muasher, and Sergio Oehninger rhe timing of im] Jantation does r de alic ) oocyte/empryo Mic romanipulation techniques In Vitro Maturation and Fertilization of Immature Oocytes: A Comparative Study of Fertilization Techniques Jiann-Loung Hwang, Yu-Hung Lin, and Yieh-Loong Tsai Cumulus cells have vitro and ICSI produces superior fertilization than IVE A Quantitative and Morphological Analysis of Oocytes Collected During 438 IVF Cycles Christiane Wittemer, Jeanine Ohl, Karima Bettahar-Lebugle, Stéphane Viville, and Israel Nisand During an IVF cycle the number ocytes, fractured z« a pellucida oocytes, and germinal vesicle-stage oocytes are related to the efficiency of treatn CONTENTS, Vol. 17, No. 1 (Continued) ANDROLOGY l The Effects of Age and Abnormal Sperm Count on the Nondisjunction of Spermatozoa H. Asada, K. Sueoka, T. Hashiba, M. Kuroshima, N. Kobayashi, and Y. Yoshimura FISH analysis of sex chromosomal and ¢ diopathic infertile a higher risk of producing XXY fetuses Chromomycin A, Staining as a Useful Tool for Evaluation of Male Fertility Farhad Golshan Iranpour, Mohammad Hosein Nasr-Esfahani, Mojtaba Rezazadeh Valojerdi, and Taki Mohammad Taki Al-Taraihi Protamine deficiency in sperm chr ANIMAL EXPERIMENTATION 67 Study of the In Vitro Maturation of Mouse Oocytes Induced by Microinjection of Maturation Promoting Factor (MPF) Hideyuki Nakano and Harumi Kubo Immature (GV-stage) mouse oocyt matured Xenopus REVIEWERS JOURNAL OF ASSISTED REPRODUCTION AND GENETICS CONTENTS Volume 17, Number 2 February 2000 REPORT OF THE 9'! ANNUAL MEETING OF THE INTERNATIONAL WORKING GROUP 75 Preimplantation Genetic Diagnosis—An Integral Part of Assisted Reproduction in Association with the 1 1"" IVF Congress, Sydney, Australia, May 10, 1999 CLINICAL ASSISTED REPRODUCTION 80 Assessment of Maximal Fertilization Rates with Intracytoplasmic Sperm Injection Sai Ma and Basil Ho Yuen The modified ICSI procedure yiek I o is compared with the standard ICSI procedure 87 A Simplified Ultrasound Based Infertility Investigation Protocol and Its Implications for Patient Management Annika Strandell, Thomas Bourne, Christina Bergh, Seth Granberg, Jane Thorburn, and Lars Hamberger \ simplified pr c f fert k t raphy is the major examination, is sufficient in one-third ANDROLOGY 93 Is Diagnostic Testicular Fine Needle Aspiration Necessary in Azoospermic Men Before Sperm Aspiration/Extraction for Intracytoplasmic Sperm Injection Cycles? Z. Zukerman, R. Orvieto, O. M. Avrech, D. B. Weiss, S. Gottschalk-Sabag, E. Bar-On, O. Rufas, I. Bar-Hava, Z. Ben-Rafael, and B. Fisch We suggest that d c TEFNA npr to IVF-ICSI procedure; when spermatozoa are detected, ryopreservation should be perfor Sex Chromosomal Analysis of Spermatozoa from Infertile Men Using Fluorescence In Situ Hybridization Naomi Nishikawa, Isamu Murakami, Katsuo [kuta, and Kaoru Suzumori Sex chromosome analysis of spe ( ¢ scence in situ hybridization and the rate of XY disomy in isthenoz GYNECOLOGY 103 High Progesterone Levels and Ciliary Dysfunction—A Possible Cause of Ectopic Pregnancy Yoav Paltieli, Izu Eibschitz, Genia Ziskind, Gonen Ohel, Michael Silbermann, and Amnon Weichselbaum Exposure of human Fallopiar ¢ t : significant decline in ciliary . beat frequency and subsequently A Randomized Prospective Cross-Over Study of Highly Purified Follicle-Stimulating Hormone and Human Menopausal Gonadotrophin for Ovarian Hyperstimulation in Women Aged 37-41 Years S. Bassil, C. Wyns, and J. Donnez In women age tion may be the best choice for a good ovarian response CONTENTS, Vol. 17, No. 2 (Continued) PHYSIOLOGY 113. Effectiveness of Low Dose of Gonadotropin Releasing Hormone Agonist on Hormonal Flare-Up Bettina Bstdndig, Isabelle Cédrin-Durnerin, and Jean Noél Hugues Hormonal flare-up after low dose Cytokine Levels in a Patient with Severe Ovarian Hyperstimulation Syndrome Before and After the Ultrafiltration and Reinfusion of Ascitic Fluid Masayuki Ito, Tasuku Harada, Tomio Iwabe, Masahiro Tanikawa, and Naoki Terakawa Measurement of serum cytokine concentrati wulation syndron GENETICS 121 Primer System for Single Cell Detection of Double Mutation for Tay-Sachs Disease Ming Chen Liu, Kenneth C. Drury, Simon Kipersztok, Wenrong Zheng, and R. Stan Williams Simultaneous amplification, de S ene (HEXA) is accomplished using s cells and a novel PCR primer systen SHORT COMMUNICATION 127 Understanding Differences in the Perception of Anonymous Parties: A Comparison Between Gamete Donors and Their Recipients Steven R. Lindheim, Suzanne Kavic, and Mark V. Sauei JOURNAL OF ASSISTED REPRODUCTION AND GENETICS CONTENTS Volume 17, Number 3 March 2000 CLINICAL ASSISTED REPRODUCTION 131 Prolonged Culture of Human Cryopreserved Embryos with Recombinant Human Leukemia Inhibitory Factor Yao-Yuan Hsieh, Horng-Der Tsai, Chi-Chen Chane, Li-Wei Hsu, Su-Chen Chang, and Hui-Yu Lo LIF significantly promotes preimplar LIF-supplemented HTF provided a similar culture environment as LIF-supplemented M# Comparison of Zona Manipulated and Zona Intact Blastocyst Transfers: A Prospective Randomized Trial A. Z. Isik, K. Vicdan, A. Kaba, and G. Dagli Enzymatic treatmetntIm en of zonaz onaf pell cidala cysts east not harmful to the embryos, but significant improvement in pregnancy and implant 110N rates cc ild ot } Does Pretreatment with Progestogen or Oral Contraceptive Pills in Low Responders Followed by the GnRHa Flare Protocol Improve the Outcome of [VF-ET? E. Al-Mizyen, L. Sabatini, A. M. Lower, C. M. Y. Wilson, T. Al-Shawaf, and J. G. Grudzinskas Pretreat owed by the GnRH-a “flare” protocol does not mprove the outcome of IVF-E1 ANDROLOGY 147 The Effect of Human Fallopian Tube Epithelium on Human Sperm Velocity Motility and Binding Genia Ziskind, Yoav Paltieli, Izu Eibschitz, Gonen Ohel, and Amnon Weichselbaum I Xposure ¢ hur y tur id not en ince the motility and velocity of the sperm, but n HZA 151 Analysis of Spermatozoa from Seven ICSI Males with Constitutional Sex Chromosomal Abnormalities by Fluorescent In Situ Hybridization J. C. Giltay, R. J. T. van Golde, and P. M. M. Kastrop FISH studies « perma ICSI tpr vidence that the risk of producing offspring with a sex chromosomal abnormality ts h rer Kk sw nality as compared to the risk for males with a PHYSIOLOGY 156 Physiological Induction of the Acrosome Reaction in Human Sperm: Validation of a Microassay Using Minimal Volumes of Solubilized, Homologous Zona Pellucida Daniel R. Franken, Hadley S. Bastiaan, and Sergio C. Oehninger Hyaluronic Acid Inhibits Apoptosis in Granulosa Cells via CD44 Tomoko Kaneko, Hidekazu Saito, Mayumi Toya, Takakazu Satio, Kenji Nakahara, and Masahiko Hiroi ei was reduced by hyaluronic acid binding to CD44 CONTENTS, Vol. 17, No. 3 (Continued) 168 Effects of Aging on Ovarian Fecundity in Terms of the Incidence of Apoptotic Granulosa cells Seved Homayoon Sadraie, Hidekazu Saito, Tomoko Kaneko, Takakazu Saito, and Masahiko Hiroi \ging increases apoptotic changes . lecreas ecundity GYNECOLOGY 174. Low-Dose Aspirin for Infertile Women with Thin Endometrium Receiving Intrauterine Insemination: A Prospective, Randomized Study Yao-Yuan Hsieh, Horng-Der Tsai, Chi-Chen Chang, Hui-Yu Lo, and Chine-Lun Chen Better pregnancy rate and endometr t ere SV 1 er aspir e the endometrii SHORT COMMUNICATIONS 178 Use of a Laser to Evaluate Zona Pellucida Hardness at different Stages of Mouse Embryonic Development In Vitro and In Vivo Markus Montag, Brita Koll, and Hans van der Ven Up to the moru on Survival and Subsequent In Vitro Development of Hamster Embryos After Exposure to Cryoprotectant Solutions Keiji Mochida, Yoshie Yamamoto, Yoko Noguchi, Kaoru Takano, Junichiro Matsuda, and Atsuo Ogura DMSO and Induction of Early Pregnancy Factor from Mouse Metoestrous Ovaries and Oviducts In Vitro by Steroid Hormones G. E. Lash and M. Legge Metoestrous ovaries and JOURNAL OF ASSISTED REPRODUCTION AND GENETICS CONTENTS Volume 17, Number 4 April 2000 CLINICAL ASSISTED REPRODUCTION 189 Effect of Clinical and Semen Characteristics on Efficacy of Ovulatory Stimulation in Patients Undergoing Intrauterine Insemination Benjamin N. Hendin, Tommaso Falcone, Jorge Hallak, Jeffrey Goldberg, Anthony J. Thomas, Jr. David R. Nelson, and Ashok Agarwal Ovulatory stimulation increases IUL ps 1g patients without a history of pelvic surgery and with good postwash spert Pentoxifylline Initiates Motility in Spontaneously Immotile Epididymal and Testicular Spermatozoa and Allows Normal Fertilization, Pregnancy, and Birth After Intracytoplasmic Sperm Injection P. Terriou, E. Hans, C. Giorgetti, J. L. Spach, J. Salzmann, V. Urrutia, and R. Roulier Pentoxifylline induces mo ticular immotile spermatozoa. It allows normal fertilization, embryo quality, pregnanc ind t { ismic sperm injection Salpingectomy for Repeated Embryo Nonimplantation After In Vitro Fertilization in Patients with Severe Tubal Factor Infertility H. Dechaud, T. Anahory, N. Aligier, F- Arnal, H. Humeau, and B. Hedon SalpI ingectomy imp:I ts witl ind irreversible tubal factor sterility who have tilure IVI Intracytoplasmic Sperm Injection Increases Embryo Fragmentation Without Affecting Clinical Outcome John L. Frattarelli, Mark P. Leondires, Bradley T. Miller, and James H. Segars When compared to IVF, ICSI pr i br n thos mbryos chosen for transfer but results in similar preg 1 implantat Iwo Different Timings of Intrauterine Insemination for Non-Male Infertility Fu-Jen Huang, Shiuh-Young Chane, Yine-Jen Lu, Fu-Tsai Kune, Meng-Yin Tsai, and Jick-Fuu Wu limed intrauteri isemil ANDROLOGY 218 The Effects of Cryopreservation on Semen from Men with Sarcoma or Carcinoma J. Hallak, A. Mahran, J. Chae, and A. Agarwal Semen quality i s will reservation. However, it ts still adequate for 1 PHYSIOLOGY Relationships Between Concentrations of Tumor Necrosis Factor-a and Nitric Oxide in Follicular Fluid and Oocyte Quality Kyu Sup Lee, Bo Sun Joo, Yong Jin Na, Man Soo Yoon, Ook Hwan Choi, and Won Whe Kim Follicular fluid TNF-a concentratior t ul yl juality and follicular fluid NO concentration was increased in women with cndomectriosis CONTENTS, Vol. 17, No. 4 (Continued) SHORT COMMUNICATIONS 229 Pregnancy After Preimplantation Genetic Diagnosis by Fluorescence In Situ Hybridization Using 18-, X-, and Y-Chromosome Probes in an Infertile Male with Mosaic Trisomy 18 Hsiao-Jui Wei, Han-Sun Chiang, Wei-Min Lin, and Jaw-Yuan Wen Repeated Oocyte Maturation Block Keith L. Harrison, Deborah A. Sherrin, and J. Douglas Keeping Successful Pregnancies in a Case of Retrograde Ejaculation Associated with Tethered Spinal Cord Syndrome Hiroaki Shibahara, Hideaki Toji, Minoru Shigeta, Tetsuro Yoshimoto, Hiroki Shima, and Koji Koyama JOURNAL OF ASSISTED REPRODUCTION AND GENETICS CONTENTS Volume 17, Number 5 May 2000 CLINICAL ASSISTED REPRODUCTION The Potential Use of Maturation In Vitro of Human Oocytes in Low Responder Patients Antonio Requena, Fernando Neuspiller, Ana C. Cobo, Marina Aragonés, José Remohi, Carlos Simon, and Antonio Pellicer In vitro oocyte mat could ( . rice yatients with a low response to gonadotropin stimulation but with a norrmn al ] numbnNehrer ¢ The Effect of Patient and Semen Characteristics on Live Birth Rates Following Intrauterine Insemination: A Retrospective Study Benjamin N. Hendin, Tommaso Falcone, Jorge Hallak, David R. Nelson, Sreenivas Vemullapalli, Jeffrey Goldberg, Anthony J. Thomas, Jr., and Ashok Agarwal Advanced age or a history of c oor postwash sperm motility predicts poor [UI success rates. Frequency and Patterns of Premature Sperm Chromosome Condensation in Oocyte Failing to Fertilize After Intracytoplasmic Sperm Injection Bernd E. Rosenbusch Reasons for fertiliza failure afte t smic sperm injection were investigated in 100 oocytes and corresponding data from tp revious Conservative Management versus Aspiration of Functional Ovarian Cysts Before Ovarian Stimulation for Assisted Reproduction Gabriel E. Fiszbajn, Rubén G. Lipowicz, Laura Elberger, Adriana Grabia, Sergio D. Papier, Santiago P. Brugo Olmedo, and Claudio F. Chillik No differences in ART results it ere found when conservative management and aspiration of functiona! Ovarian cysts present bel The Prognostic Value of Age and Follicle-Stimulating Hormone Levels in Women over Forty Years of Age Undergoing In Vitro Fertilization Andrew H. Watt, Anna T. R. Legedza, Elizabeth S. Ginsburg, Robert L. Barbieri, Robert N. Clarke, and Mark D. Hornstein rhe clomiphene citrate challenge test be used to predict IVF outcome in women over age 40 PHYSIOLOGY 269 The Use of a Combined Regimen of GnRH Agonist Plus a Low-Dose Oral Contraceptive Improves the Spontaneous Pulsatile LH Secretory Characteristics in Patients with Polycystic Ovary Disease After Discontinuation of the Treatment Alessandro D. Genazzani, Cesare Battaglia, Ombretta Gamba, Felice Petraglia, Barbara Malavasi, and Andrea R. Genazzani The administration of GnRHa plus A se oral contraceptive restores an appropriate gonadotropin release in PCOD patients CONTENTS, Vol. 17, No. 5 (Continued) GENETICS 276 Fish Analysis of Six Chromosomes in Unfertilized Human Oocytes After Polar Body Removal Elena Martini, Sean P. Flaherty, Nicholas J. Swann, Colin D. Matthews, Frans C. S. Ramaekers, and Joep P. M. Geraedts Repeated FISH analysis and trequenc human oocytes after polar body human oocytes after polar body ren Human Embryos Derived from In Vitro and In Vivo Matured Oocytes: Analysis for Chromosomal Abnormalities and Nuclear Morphology Constance DeScisciolo, Diane L. Wright, Jacob F. Mayer, William Gibbons, Suheil J. Muasher, and Susan E. Lazendorf Compared to ¢ wari SHORT COMMUNICATIONS 293 Paternal Paracentric Inversion of Chromosome 2: A Possible Association with Recurrent Pregnancy Loss and Infertility Denise H. Devine, Gail Whitman-Elia, Robert G. Best, and Janice G. Edwards Menses Predict Successful Ovarian Down-Regulation Using GnRH-a Prior to IVF-ET Suzanne Kavic, M. V. Sauer, and S. R. Lindheim Reduction of Human Menopausal Gonadotropin Dose Before Coasting Prevents Severe Ovarian Hyperstimulation Syndrome with Minimal Cycle Cancellation Mohamed A. Aboulghar, Ragaa T. Mansour, Gamal I. Serour, Catherine A. Rhodes, and Yahia M. Amin