IS 6385: Saccharin, Food Grade

10 Pages·1997·1.1 MB·English

by  Bureau of Indian Standards

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“The Right to Information, The Right to Live” “Step Out From the Old to the New”
IS 6385 (1997): Saccharin, Food Grade [FAD 8: Food
Additives]
“!ान $ एक न’ भारत का +नम-ण”
Satyanarayan Gangaram Pitroda
““IInnvveenntt aa NNeeww IInnddiiaa UUssiinngg KKnnoowwlleeddggee””
“!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता हहहहै””ै”
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“Knowledge is such a treasure which cannot be stolen”

IS 6385:1997

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Indian Standard
SACCHARIN, FOOD GRADE
SPECIFICATION
( Second Revision)
ICS 67.220.20
CBIS 1997
BUREAU OF INDIAN STANDARDS
MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARO
NE W DELHI 110002
September 1997 PriceGroup 2
AMENDMENT NO. 1 FEBRUARY 2006
TO
IS 6385: 1997 SACCHARIN,FOOD GRADE
{SPECIFICATION
:(SecondRevision)
LPage2,TableI,SfNo (I),coil]- Substitute~99'for'98'
(FAD8)
ReprographyUOit.81S,NewDeihl,tiaa
FoodAdditivesSectionalCommittee,FAD8
FOREWORD
ThisIndianStandard(SecondRevision)wasadoptedbytheBureauofIndianStandardsafterthedraft
finalizedbytheFoodAdditivessectionalCommitteehadbeenapprovedbytheFoodandAgriculture
DivisionCouncil.
Saccharinisanon..nutritivesweetener. ItIsnormallyusedbydiabeticsandthosewhoneedlowcalorie
dietasasubstituteforcanesugar. Itisabout500timessweeterthansugar.
Useofsaccharinfoodgradeasartificialsweetenerhasbeenpermittedinselectedfooditemsunderthe
PreventionofFoodAdulterationRules,1955.
Thisstandardwasfirstpublishedin1971andwasrevisedin1978.Itisbeingrevisedtomakethefollowing
changes/additions:
a) Toprovideaseparateclausefordescriptionincorporatingthesolubilitypropertiestokeepthe
standardinlinewiththeFoodChemicalCodex,NRC.
b) Toreducethelimitfortoluenesulfonamides.
c) Tosubstitute therequirement ofleadbyheavymetalsandthecorrespondingchangesin test
method.
d) Toincludedirectionsforstorageandexpirydateunderthemarkingclause.
ChemicalNamesandFormula-Therecognizedchemicalnamesare0-benzosulfimide;2,3dihydro-3
oxobenzisosulfonazole;and1,2..benzisothiazole-3-one-l,I-dioxide.ItsempiricalformulaisC7HsNO)S.
Molecularweightis183.18andstructuralformulaisasunder:
o
~~
~S02
Inpreparationofthisstandard,considerableassistancehasbeenderivedfromtheroHowingpublications:
CompendiumofFoodAdditiveSpecifications,Volume2,Joint FAO/WHOExpertCommitteeon
FoodAdditives(JECFA), 1992.
Specificationsforidentityandpurityofsomefoodadditives,1975.FAO/WHO,Rome.
FOOdChemicalCodex.ThirdEdition. NationalAcademyofSciences,NationalResearchCouncil,
WashingtonDC.USA.
ThisstandardisharmonizedwiththestandardofFAO/WHO.
Forthepurposeofdecidingwhetheraparticularrequirementofthisstandardiscompliedwiththefinal
value,observedorcalculated,expressin,theresultofatestoranalysJs.&hallberoundedoffinaccordance
withIS2:1960'Rules forroundingoffnumericalvalues(revised)'. Thenumberofsignificantplaces
retainedintheroundedoffvalueshouldbethesameastbatofthespeclfledvalueinthisstandard.
IS6385:1997
Indian Standard
SACCHARIN, FOOD GRADE
SPECIFICATION
(Second Revision)
1 SCOPE todrynessandgentlyfusetheresidueoverasmall
flameuntilitnolongerevolvesammonia.Afterthe
Thisstandardprescribestherequirementsandthe
residuehascooled,dissolveit in20mlofwater.
methodsofsamplingandtestforsaccharin.food
neutralize the solution with dilute hydrochloric
grade.
acidandfilter. Theadditionof adrop offerric
Z REFERENCES chloride solution (9 g of ferric chloride and
sufficientwatertomake100ml)tothefiltrateshall
ThefollowingIndianStandardscontainprovisions
produceavioletcolour.
whichthrough reference in this text. constitute
provision of this standard. At the time of 4.1.3Mix20mgofthematerialwith40mgofthe
publication,theeditionsindicatedwerevalid.All resorcinol,add10dropsofconcentratedsulphuric
standards are subject to revisionand parties to acidandheatthemixtureinaliquidbathat200°C
agreementsbasedontbisstandardareencouraged for3minutes.Aftercoolingadd10mlofwaterand
toinvestigatethepossibilityofapplyingtbemost anexcessof10percentsodiumhydroxidesolution.
recenteditionsofthestandardsindicatedbelow: Afluorescentgreenliquidshallbeproduced.
[SHo. Title 4.1.4 Melting range of the material shall be
1070:1992 Reagent grade water (third between226and2300C.
revision)
NOTB - Meltinapoint is the temperatureat which
1699:1995 Meth~ofsamplingandtestfor liquefactionofthe.ubltanceoccun,whichisindicatedbythe
synth,tic food colours (second formationofadefinitemenilcus. Themellinlpointofthe
substanceshouldrailwithintheraneespecified.
rev~)
S34S:1978 Sodi.'" saccharin, food grade 4.2 The material shall also conform to the
(first¥Sion) requirementsgiveninTable1.
3 DESCRIP110N 5 PACKING.~ORAGEANDMARKING
1 .
3.1 Itshallbeintheformofwhitecrystalsorwhite S.l Pacldna
crystallinepowder. Itshallbeodourlessorhaving ThematerialS~llbefilled incontainerswithas
afaintaromaticodour. IthasIntenselysweettaste. littleairspace possible.Thecontainershallbe
The material shall be slightlysoluble in water, suchastoprec decontaminationofthecontents
sparinglysoluble in ethanol, slightlysoluble in withmetalsorttherimpurities.
chloroformandether andisreadilyabsorbedby
dilute solution of ammonia, solutions of alkali 5.2 Storage
hydroxidesorsolutionsofalkalicarbonateswith
Thematerialshallbestoredinacoolanddryplace
theevolutionofcarbondioxide. soastoavoidexcessiveexposuretoheat.
NOTH- The IOIubililyIIintendedonly.information
reprdinaapprolimatesolubilityandisnottobeCODIidered S.3 Marldna
at• quality MluirementandisofmiDOf'lipificanceasa 5.3.1Eachcontainershallbelegiblyandindelibly
mean. of identificationor determination of purityand
depeDdeacemustbeplacedonother.pecificatlons. markedwiththefollowinginformation:
a) Nameofthematerial, includingthewords
4 REQUIREMENTS
'FoodGrade';
4.1 Identification b) Nameandaddressofthemanufacturer;
4.1.1 A saturatedaqueoussolutionofsaccharin c) Netcontent,whenpacked;
shaHbeaddletoUlmus. d) Batchofcodenumber;
e) Directionforstorage;
4.1.2 Dissolveabout100mgofthematerialin5mt
t) Expirydate;and
ofSpercentsodiumhydroxidesolution.Bvaporate
186385:1997
h) Any otherrequirementsasspecified under granted to manufacturers or producers may be
the Standards ofWeights and Measures obtainedfromtheBureauofIndianStandards.
(Packaged Commodities) Rules, 1977/
PreventionofFoodAdulterationRules,1955. 6 SAMPLING
6.1 The representative samples of the material
5.3.2 BISCertificationMarking shallbedrawnaccordingtothemethodprescribed
in4ofIS1699.
TheproductmayalsobemarkedwiththeStandard
7 QUALIlYOFREAGENTS
Mark.
Unless specified otherwise, pure chemicals and
5.3.2.1 The useofthe Standardmarkisgoverned
distilled water (seeIS1070) shall beemployedin
bytheprovisionsofBureauofIndianStandardsAct,
tests.
1986 and the Rules and Regulations made
thereunder. Thedetailsofconditionsunderwhich NOTE- 'Purechemica'"thallmeanchemicalsthatdonot
the licence for the use ofStandardMarkmaybe containimpuritiea.whicbaffecttheresultsofanalysis.
Table1 RequirementsforSaccharin,FoodGrade
(Clause4.2)
SI Charaderlsllc Requirement Method01Test,Refto
No. t '\
Annex IS1699 ISS34S
ofThis
Standard
(1) (2) (3) (4) (5) (6)
i) PurityasC7115N03S,percent 98 A-I
bymass (ondrybasis),Minmass
ii) Moisture,percentbymasson 1.0
dryingatlOSoCfor2hours,Max
iii) Benzoicandsalicylicacids Topautbe A-2
test
iV) Readilycarbonizable Topasathe A-S
substances test
v) Sulphatedash,percentbymau,MtrC 0.2 A-3
vi) Toluenesulfonamidea, mglkg,Max 2S A-4
vii) Heavymetals(asPb),mg/kg,Max 10 A-oS
viii) Arsenic(as~), mglkg,Max 3 15
ix) seleniumas(Se),maika,Max 30

2
IS6385:1997
ANNEX A
(Table 1)
METHODSOFTEST.~ORSACCIIARIN,FOODGRADE
A-I PURITY A-Z TEST FOR BENZOIC AND SAUCYLIC
ACID
A-I.O Two methods have been specified for. .
determination of purity. In case of dispute, . A-2.1 DISSolve0.5 g of the sample In 10 ml of
Method 2 shall be regarded asthe reference boiling water and 3 drops of 9 percent ferric
method. chloride. No precipitate or violet colour shall
appear.
A-I.l Method1
A-3 SUI..PlIATEDASII
A-I.1.1 Reagents A.3.1 Apparatus
A-l.t.t.t Sodiumhydroxidesolution- 0.1N. A-3.1.1Flat-BottomDish- ofsilicaorplatinum.
A-t.1.1.2 Phenolphthaleinindicator A-3.1.2 Muf11e Furnace- maintained at 550 ±
20°C.
A-1.1.2 Procedure - Dissolve about 0.5 g of
previously dried (105°C for 2 hours) sample, A-J.1.3 Desiccator
accuratelyweighedin75mlhotwater.Coolquickly,
A-3.2 Procedure
add phenolphthalein indicator. Titrate with
sodiumhydroxidesolution. EachmillilitreofO.lN Weigh accuratelyabout3gofthematerialin the
sodium hydroxide is equivalent to 18.32 mgof dish, previouslydriedinanair-ovenand weighed.
saccharin(C7HsNOJS). Heat the dish gentlyona flameat first andthen
stronglyinamumefurnaceat550± 200etilJgrey
A-1.2 1\fethod2 ashresults. Cool thedishinadesiccator. Moisten
theresiduewithonemillilitreofsulphuricacidand
A-t.2.1 Reagents cautiouslyheatthedishagainat550±200efor30
A-l.2.1.1 Sodiumhydroxidesolution - 30percent minutes. Coolthedish inadesiccatorand weigh.
(m/v)inwater,andN/lO. Repeat this process ofheating for 30 minutes,
coolingandweighinguntilthedifferencebetween
A-1.2.1.2 Hydrochloficacid- concentrated.
twosuccessivewelghingsislessthan1mg. Record
A-1.2.1.3 Sulphuricacid- N/lO. thelowestDlISS.
A-I.2.1.4 Methylredindicator - Dissolve1gof A-3.3 Cal~tloD
methyl redin100mlof95percentalcohol. Sulphated~,, percentbymass= lOOM(tM~MM)
A-I.2.2 Procedure
where
Transferabout540mgofpreviouslydriedat 1050 M = massingoftheemptydish.
for2hoursandaccuratelyweighedsample, tolong Ml == massingorthedishwiththematerial
neckedflask,havingacapacityof 200mland add takenforthetest,and
toit 10mlof30percent(m/v)solutionofsodium M2 == massingofthedishwith theash.
hydroxide. Boiloverasmallflame for 2minutes,
avoiding loss byevaporation; cool,add 15mt of A-4 DETERMINATIONOFTOLUENE
hydrochloricacid and boil again for SO minutes SULFONAMIDES
under a reflux condenser. Cool, rinse the
A-4.1 Anyofthetwomethodsgiven underA-6of
condenserwith SOmlotwater,and passacurrent
ISS34Smaybeusedexceptthat1Spercentsodium
ofair through the flask to removeacid vapour.
bicarbonatesolutionshouldbe usedinplaceof5
Connectwith-anammonia distillationapparatus,
percentsolution intestpreparation(A-6.1.1.5of
add 20mlof30percent(m/v)solutionofsodium
hydroxide,and distil the ammonia into 40mtof IS5345)inMethod1.
sulphuricacid;titratetheexcessofacidwith N/I0 A-5 REAVYMETALS
sodiumhydroxideusing solutionofmethylredas
indicator. EachmillilitreofN/10 sulphuricacid, Prepareand testa2-1 sampleasperthemethod
neutralizedby,the ammoniain thedistillate, is givenat 16ofIS1699using20PIofleadIon(Pb)
equivalentto 18.32mgofsaccharin(C7H.sN03S). inthesolutionA.
3
BureauofIndinnStundards
BIS isastatutoryinstitutionestablishedunder the BureauofIndian StandardsAct, 1986 to promote
harmoniousdevelopmentoftheactivitiesofstandardization,markingandqualitycertificationofgoods
andattendingtoconnectedmattersinthecountry.
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without the priorpermission inwritingof DIS.Thisdocs not precludethefrcc usc, in thecourseof
implementingthestandard,ofnecessarydetails,suchassymbols andsizes. typeOfgradedesignations.
Enquiriesrelatingtocopyrightheaddressedto the Director(Publications),BIS.
ReviewofIndianStandards
Amendmentsarc issued to standardsas theneed ariseson the basis ofcomments. Standardsarealso
reviewedperiodically;astandardalongwith amendmentsi~reaffirmedwhensuchreviewindicatesthat
nochangesarcneeded;ifthe reviewindicatesthatchangesarcneeded,itistakenupforrevision.Users
ofIndianStandardsshouldascertainthat theyarcinpossessionofthe latestamendmentsoreditionby
referringtothelatestissueoftBTSHandbook'and'Standards:MonthlyAdditions',
ThisIndianStandardhas beendevelopedfrom Doc: No. FAD 8(717),
AmendmentsIssuedSincePubllcatlon
Amend No. DateofIssue Text Affected
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