IS 2558: Ponceau 4R, Food Grade

11 Pages·1994·1.2 MB·English

by  Bureau of Indian Standards

इंटरनेट मानक
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timely dissemination of this information in an accurate manner to the public.
“जान1 का अ+धकार, जी1 का अ+धकार” “प0रा1 को छोड न’ 5 तरफ”
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“The Right to Information, The Right to Live” “Step Out From the Old to the New”
IS 2558 (1994): Ponceau 4R, Food Grade [FAD 8: Food
Additives]
“!ान $ एक न’ भारत का +नम-ण”
Satyanarayan Gangaram Pitroda
““IInnvveenntt aa NNeeww IInnddiiaa UUssiinngg KKnnoowwlleeddggee””
“!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता हहहहै””ै”
Bhartṛhari—Nītiśatakam
“Knowledge is such a treasure which cannot be stolen”

IS 2558 : 1994
Reaffirmed 2009
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Indian Standard
PONCEAlJ 4R, FOOD GRADE - SPECIFICATION
( Second Revision)
SecondReprintNOVEMBER 1997
UDC644.099.62 :668.811.1 PON
co
BIS 1994
BUREAU OF IN'DIAN STANDARDS
MANAKBHAVAN,9BAHADURSHAHZAFARMARG

NEWDELHI 110002
PriceGroul) 2
October 1994
AMENDMENT NO.1 MAY 2008
TO'
IS 2558 : 1994PONCEAU4R,FOOD GRADE
SPECIFICAnON
(S«o.dRn.1D1I)
[Page 1,Table1,SINo. (i),col3]- Substitute '8S'/01''82'.
[Page 1,Table 1,SlNo. (iii),col3]- Substitute'0.2'for '0.4'.
[Page 1,Table1,SlNo. (iv),col3]- Substitute '0.2'for '0.4'.
(FAD 8)
RCiH'Oii"IPhYunit,aiS,NewDelhlliidia
Food Additives Sectional Committee. FAD 8
FOREWORD
This Indian Standard ( Second Revision) was adopted by the Bureau of Indian Standards. after the
draft finalized by the Food, Additives Sectional Committee had been.approved bythe Food and
Agriculture Division Council. .
This standard is one ofthe series of Indian Standards on synthetic food colours permitted under the
Prevention of Food Adulteration Rules, 1955 issued by the Ministry of Health,Government of India.
These rules, inter-alia prescribe:
'All food colours including natural colouring matter and permitted synthetic food colours and
their preparations or mixtures excluding saffron and curcumin shall be sold only under the
DIS Certification Mark.'
'Indian Standard specification for ponceau 4 R' was first issued in 1963. It was revised in 1974 to
bring it in line with the FAO/WHO specifications and also takinginto account the indigenous data
generated. This standard is being revised again taking into consideration the latest specification for
food colour issued by FAO/WHO [see FAO Food and Nutrition Paper 31/1 ( 1984 ) 'Specification for
identity and purity offood colours' ], the latest specifications laid down under Food Chemical Codex.
EEC Directives, Canadian Food Laws and Food and Drugs Act of USA. In addition, due consideration
bas been given to indigenous data.
In this revision the minimum requirement for total dye content has been raised and the maximum
requirement of loss on drying at 135°C and chloride and sulphate expressed as sodium salt has also
been increased. The limits for water insoluble matter and combined ether extracts have been made
more stringent although, the requirements have still not been aligned with the International require
ments as the industry expressed inability to follow the strict International requirements. The
Committee, however, decided to collect data on the parameter and review the limits at a later
stage. '
Requirements for Ponceau 4 R, food grade have been prescribed under the PFA Rules. As food
colours are under compulsory BIS Certification, the modified values given in Table 1 have been
referred to CCFS for adoption under the PFA Rules. While formulating this standard, necessary
consideration has been given to the Standards of Weights and Measures (Packaged Commodities)
Rules, 1977. The standard is however subject to restrictions imposed under these Rules, wherever
applicable.
DescriptioD
Common Name- Ponceau 4 R.
Synonyms - C.I. Food Red 7, L·Rot No.4, Coccine Nouvelle, Cochineal Red A; E.E.C. Serial
No. E 124.
Colour ofthe 0·1 Percent ( m/v ) Solution InDistilledWater - Red.
Colour IndexNumber ( 1975 ) - No. 16255.
class- Monoazo.
Chemical Name·- Trisodium salt of 1· ( 4· sulpho • 1· naphthylazo ) • 2· naphthol • 6, 8·
disulphonic acid.
Empirical Formula - CIOH11N.Ol0S.Na••
Molecular Weight - 604·5.
( Contlnu,don thirdcov., )
IS1558:1994
Indian Standard
PONCEAU 4R, FOOD GRADE ~ SPECIFICATION
( Second Revision)
1SCOPE 3.2Freedomfro..Contaminants
1.1 Thisstandardprescribes the'requirements and the Precautionsshallbetakentoensurethatthematerialis
methodsofsamplingandtestforPonceau4R,foodgrade. free from mercury, selenium and cbromimumin any
fonn; aromatic amines, aromatic nitro compounds,
Z REFERENCES
aromatichydrocarbons,and cyanides.
ThefollowingIndianStandardsarenecessaryadjuncts 3.3Theproductshallbeprocessed,packed,storedand
to this standard. distributed under hygienic conditions in licenced
ISNo. Title premises(see IS2491 : 1972).
1070: 1992 Reagentgradewater(thintrevision)
4 PACKINGANDMARKING
1699 : 1994 Methods of sampling and test for
food colours (secondrevision) 4.1 Packing
2491 :'1972 Code for hygienic conditions for
The material shall be packed in glass containers,
foodprocessingunits(firstrevision)
polyethylenecontainer,metalcontainers, orcardboard
3 REQUIREMENTS containerssuitablylinedwithpolyethylene. Subject to
agreement between the purchaser and the vendor any
3.1 The material shall conform to the requirements
othersuitablecontainer tnayalsobe used.
prescribed inTable 1.
Table 1Requirementsfor Ponceau 4R
(Clause3.1)
SINo. Characteristic Requirement MethodofTest, Refto
"AnnexofThis Clauseof
Standard IS 1699 : 1994
(1) (2) (3) (4) (5)
i) Totaldyecontent,correctedfor 82 A
sample dried at 105:t1°Cfor
2h,percentby mass,Min
ii) Lossondryingat 135°C,per 18 6
centbymass,Max
and
Chlorides and sulphatcs ex 13
pressedassodiumsalt,percent 14
bymass,Max
iii) Water-insolublematter, percent 0.4 7
bymass,Max
iv) Combined ether extracts per 0.4 8
centbymass,Max
v) Subsidiary dyes, percent by 1.0 B
mass,Max
vi) Dye intermediates, percent by O.S c
mass,Max
vii) .Lead,mg/kg,Max 10 15
viii) Arsenic,mg/kg,Max 3 15
ix) Heavymetals,mg/kg,Max 40 16
IS1558:\1994
4.% Marking 4.2.%.1 Theuse of theStandardMarkisgovernedby
theprovisions oftheBureauofIndianStandardsAct,
4.%.1 Each container shall be legibly and indelibly 1986andtbeRulesandRegulationsmadethereunder.
markedwiththefollowinginformation: Thedetails ofconditions underwhicbthe licencefor
a) The words 'FOODCOLOUR'; tbeuseofStandard Markmaybegrantedto manufac
b) Conunon nameoftbe colour; turersor producers may be obtained fromtheBureau
ofIodia'nStandards.
c) Cheemicalnameoftbecolour;
S SAMPLING
d) Colourindex number;
e) Sourceofmanufacture; S.1 Representative samples ofthe material for tests
t) Date ofInanufacture; shallbedrawnandcriteriaforascertainingconformity
totherquirements of tbisspecificationsballbedeter
g) Nameofmajordyeintermediates;
mined according to the method prescribed in 4 of IS
b) Minimumnetmassingramsor kilograms; 1699: 1994. .
j) Batcborcodenumber;and
'TESTS
k) Any otherrequirementsas specifiedundertbe
Standardso/WeightsandMeasures(Pac/cQged .6.1Testsshallbecarriedoutasprescribedincol4and
Commodities) Rules, 1977/PreventionofFood 5.of Table 1.
AdulterationRules, 1955.
° 6.1 QualityofReagents
4.z.z SISCertificationMarking Unlessspecifiedotherwise, purechemicalsanddistilled
water(seeIS1070:1992)shallbeemployedintests.
The product may also be marked witb tbe Standard NOTE- 'Purechemicals"shallmeanchemicalsthatdo
Mark. notcontainimpuritieswhichaffecttheresultsofanalysis.
ANNEXA
[Table 1,Item (i) ]
DETERNUNATIONOFTHETOTALDYECONTENT
. A·OGENERAL the dye-sample and dry tbis in an air-oven at
105:t 1°C for 2 hours. Calculate tbe Jossofmass on
Two methods, spectrophotometric metbod and the
drying; from tbisdata, calculatetbe dry mass of tbe
titanium trichloride metbod,are specified. In case of
sample(M)inthefinalsolutiontakenformeasurement
dispute,spectrophotometricmethodshallberegarded
ofopticaldensity. .
asthereferencemetbod.
A·I.3Calculation
A-I SPECTROPHOTOMETRICMETHOD
=
Totaldyecontentintbe sample,percentby mass
A-I.IApparatus
ODx 100
Suitable spectropbotometer witb properly calibrated
Mx440
scalesfor botb wavelengtb and optical density.How
ever, a suitable spectrocolorimeter maybe usedafter wbere
proper calibration against a spectrocolorimetermay OD = opticaldensityfound;
be used after proper calibration against a M = dry massofthe sample in100mJsolution;
spectrophotometer.
and
A-I.%Procedure 440 = £1%1 em,506IUD, for Ponceau4Rin0.1 N
hydrochloricacid.
Weigbaccuratelyabout250mgoftbedyesampleand
dissolve in 0.1 N hydrochloric acid in 250-mJ
A·2 TITANIUMTRICHLORIDEMETHOD
volumetric flask. Dilute this further with 0.1 N
hydrochloric acid to make a tinal concentrationof 1
A-Z.IPROCEDURE
mg/IOO mJ (approximately). Findouttbeopticalden
sity oftbis dilutedsolution,against0.1 Nbydrochloric The method given in 5 of IS 1699 : 1994shall be
acidasblank,..t506nm,inaglasscellwitb 10.0 mm followed. Thepercentageoftotal dyecontentsballbe
lightpatb. determinedusingtbefoJJowingcalculations:
A-l.2.1 Simultaneouslyweigbaccuratelyabout2gof 1mJorO.1 Nn03.0.015 11gofPonceau·4R.
2
IS 2558: 1994
ANNEX 8
[Table 1,Item (v) ]
DETERMINATIONOFSUBSIDIARYDYECONTENT
B·I PROCEDURE Developingsolvent =No.3
=
The method given in 9 of IS 1699 : 1994 using the Heightofascentof 17em, then one hour
conditionsgivenbelowshallbefollowed: solvent front forfurtherdevelopment.
ANNEX C"
[ Table 1,Item (vi) ]
DETERMINATIONOFDYEINTERMEDIATES
e-o Two methods, column chromatographic and b) Prepareon 100percent basis, mlvsolution'of
ascending paper chromatographic method have been 2-Napbthol6:8DisulpbonicAcidinamixture
specified. In case of dispute tbe column ofoinepartsofwaterandonepartofammonium
chromatognphic method shall be regarded as the hydroxide, so that 5111 of the solution shall
referencemethod. correspondto0.3 percentinthedyesample.
c-r METHODI C-l.3.Z TestSubstances
C-I.3.1.1 Dyesolution
C-I.OPrinciple
50 ~I of tbe solution sbaII be equivalent to 1 000
Ponceau 4R is prepared by diazotization of I-nap
microrgamsoftbesample.
thylamine-4-sulphonicacidandtbediazosoformed is
coupled with 2-naphthol6 :'8 disulphonic acid.After C-I.3.Z.2Referencesubstances
thecompletionoftbereactionthetracesoftheseinter a) I-Naphthylamine-4-SulphonicAcid,and
mediates remain,whichcanbeestimatedbyascending b) 2-NaphthoI6:8DisulphonicAcid.
papercbromatography.
NOlc - Different concentrations ofdye intermediates
e-I.1Apparatus maybeprepared,ifnecessary.
C-I.l.l Chromatogrphy Tank and Ancillary Equip C-l.3.3 Mark outasheetofchromatographicpaperas
ment- asgivenunder9.2.1 of IS 1699:1994. showninFig.3 of IS 1699: 1994andspot sufficient
e-l.t.2 Microsyringe - capable of 0.2 ml with a quantityof dyesampleas unifonnly as possible with
toleranceof:t0.0002mI. thehelpofmicrosyringe. Alsospotreferencesubstan
ces(C-l.3.Z.2).Beforemountingthesheetofpaper,cut
C-I.I.3 UltravioletLamp - at365.5 nm.
otIsomeportionoftbepaperbelowthebaselinetotake
C·l.1.4 FilterPaper- WhatmanNo.1 orequivalent. careofanyspreadingofthe initialspot. Poursufficient
developingsolventintothetanktobringthesurfaceof
C·l.ZReagents
the solvent just below the cut portion of tbe
C-I.2.1AmmoniumHydroxideSolution- sp gr0.923. chromatographicpaper.Take outthe filterpaperafter
C·I.2.2 l-Naptuhylamine-t-SulphonicAcid thedevelopingsolventhassufficientlymovedup.Dry
C-l.2.3 2-NaehlhoI6:8DisulphonicAcid inanovenat70 to750 Corbybairdrier.
C-l.2.4 Developing Solvent - Sodium bicarbonate C-1.3.4DetectionandComparisonoftheDerivatives
solution(10 percentm/v). C-l.3.4.1 Detectionandsemi-quantitativeestimation
e-l.3 Procedure Inspect the cbromatogram under ultra violet light.
C-l.3.1 PreparationofSolutions Compare visuallythe intensityof tbe Ouorescenceof
thesamplewithtbereferencesubstanceandreporttbe
C-l.3.1.1 Prepare2percentmlv solutionofthedyein
contentof l-Naphthylamine-4-Sulphonic Acid,and2
a mixture of nine parts of water and one part of the
Naphthol6:8DisulpbonicAcidwhichshallbenearest
ammoniumhydroxide.
inintensitytooneofthe correspondingreferencesub
C-l.3.I.% Reference Substances stances.
a) Prepareon100percentbasis,mlvsolutionof1
Napbtbylantine-4-SulpbonicAcidin8 mixture C-%METH6nz
ofninepartsofwaterandonepartofammonium
to
hydroxide, so that 5 f.ll of the solution shall The column chromatographic method givenin of
correspond to 1.5percentintbedyesample. IS 1699 : 1994shallbefollowed.
3
St~ctu,al For~"'a
OH
N=N
So/ubility - Soluble in water.
Sparingly soluble in ethanol.
For the purpose ofdeciding whether a particular requirement of this standard is complied with, the
final value, observed or calculated, expressing the result ofa test or analysis, shaIIbe rounded otTin
accordance with IS 2 : 1960 'Rules for rounding off numerical values (revisedf. The number of
significant places retained in the rounded off value should be the same as that of the specified value in
this standard.