IS 1698: Indigo Carmine, Food Grade

11 Pages·1994·0.98 MB·English

by  Bureau of Indian Standards

इंटरनेट मानक
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in order to promote transparency and accountability in the working of every public authority,
and whereas the attached publication of the Bureau of Indian Standards is of particular interest
to the public, particularly disadvantaged communities and those engaged in the pursuit of
education and knowledge, the attached public safety standard is made available to promote the
timely dissemination of this information in an accurate manner to the public.
“जान1 का अ+धकार, जी1 का अ+धकार” “प0रा1 को छोड न’ 5 तरफ”
Mazdoor Kisan Shakti Sangathan Jawaharlal Nehru
“The Right to Information, The Right to Live” “Step Out From the Old to the New”
IS 1698 (1994): Indigo Carmine, Food Grade [FAD 8: Food
Additives]
“!ान $ एक न’ भारत का +नम-ण”
Satyanarayan Gangaram Pitroda
““IInnvveenntt aa NNeeww IInnddiiaa UUssiinngg KKnnoowwlleeddggee””
“!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता हहहहै””ै”
Bhartṛhari—Nītiśatakam
“Knowledge is such a treasure which cannot be stolen”

IS1698:1994
(R-.-l_)
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Indian Standard
INDIGO CARMINE, FOOD GRADE
SPECIFICATION
( Second Revision)
FirstReprintJUNE200I
UDC 664.099.62:667.12.6.2
©BlS 1994
BUREAU OF INDIAN STANDARDS
MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARG
NEW DELHI 110002
PriceGroup 2
November 1994
AMENDMENTNO.1 MAY 2008
TO
IS1698:1994INDIGOCARMINE,FOODGRADE
SPECIFICAnON
(SecondRevision)
[Page1,Table I,SlNo.(iii),col3]-Substitute '0.2'for '0.4'.
[Page1,Table I,SINo.(iv),coI3]-Substitute '0.2'for 'OA'.
[PageI, TableI,.'IINo.(v),col3J- Substitute '1.0'for '3.0'.
[PageI,Table I,SINo.(vi),co/3]-Substitute '0.5'for'I'.
(FAD8)
RcprographyUnit.SIS,NewDeihl,India
FoodAdditivesSectionalCommittee,FAD8
FOREWORD
ThisIndianStandard(SecondRevtston)wasadoptedbytheBureauofIndianStandards,afterthe draft
finalizedbythe FoodAddmvesSectionalCommitteehad beenapprovedbythe FoodandAgricultural
DIVISionCouncil,
TIu~standardISoneoftheseries ofIndianStandardsfor ediblesyntheticcolourspermuted under the
PrevenuonofFoodAdulterattonRules,1955ISSuedbytheMinistryofHealth,GovernmentofIndia.These
rules, Inter-aha prescnbe:
'All food coloursincludingnaturalcolounngmatterandpermittedsynthetic food coloursand
theirpreparatIonsormixturesexcludmgsaffronand curcurrunshallbesoldonly underthe BIS
CcrufuauonMark'
'IndianStandardspecincauonformdigocarmrnc'wasfirst ISSUed10 1960 Itwasrevised10 1974tobnng
n10tinewnhtheFAO/WHOspecrncauons ThrsstandardISbemgrevisedagaintakingintoconstderauon
the tates: pubhcauon for the food colour Issued byFAO/wHO [seeFAO Food and Nutnuon Paper
,I/I(1'184)Specifuauonforrdenutyand pUrtlyoffoodcolours),thelatestspecifuauonslaiddown under
FoodChemicalCodex,EECDirecuves,CanadianFoodLaws,Foodand DrugsAuofUSA Inaddiuon.
dueconsiderauonhas beengiven to rndrgenousdata
Inthis revision the limnsforwaterinsolublematter.combinedetherextractsanu subsidiarydyes have
beenmademorestringenttoalign themwnhthe lnternauonalreqUirement\andrequirements10' heavy
metalshave beenmcluded
Requirements for mdigo carrnme, food grade have been prescribed under the Prevenuon vi Food
AdulterattonRules. 1'155 As food coloursarc undercompulsoryBISCertifrcauon,the modifiedvaluc-,
gIVen10TableIhave been referred10CCFSforadopuonunderthe Pf-A Rules While Iorrnutaungthl'
standard,necessdryconsideranon has beengiven to the StandardsofWe/ghB andMeasures (Packaved
Commoduies)Rules. 1'177ThestandardIShoweversubjectto restricuonsImposed underthese Rules,
whereverapplicable
Description
CommonName- Indigocarmme
Synonymr- Indrgoune,FD& CBlue No 2.CI FoodBlue I,EEC Send) No E1'2.L Blau2
Colourofth»0IPercent(mtv]SoluuonIn DtsutledW(/{er Blue
ColourIndex Number(1975) - No 710)5
Cluvs IndlgOid
ChennculName --DisodrumsdlLIIImdrgoune 5'y-dJsulphonlldud
Empmcalformula
MolecularWe/Khl - 4h6 ,6
SITU,turalFormula
Solutnluy SolubleInwater
SparlnglvvolubleInethanol
forthe purpo,e01 deudlngwhetheraparucularreqUiremenlot thr-standardIScompliedwuh,the hndl
value,observedorcalculated,cxprcwmgthe resultofatestordnaIY"ls.shall bcroundedoffInaccordance
wuh IS2 1960 Rulev for roundingolfnumenwlvalues(re'lIed)' The number of"gmhwnl pld<-C'>
retainedInthe roundedoffvalueshouldhethe'dmed'IhdlofIhe,peufteuvalue Inthixstandard
IS1698:1994
Indian Standard
INDIGO CARMINE, FOOD GRADE
SPECIFICATION
( Second Revision)
I SCOPE 2491:1972 Code for hygienic conditions for
1.1 This,tanllarllpre:.cribcstherequirement,and foodprocessingunits(firs!revision)
themethods of sampling and testforindigocar
3 REQUIREMENTS
mine,foodgrade.
2 REFERENCES 3.1 The material shall conform to the require
The following Indian Standard,ale necessaryad mentsprescribedinTable1.
juncts tothisstandard:
3.2 FreedomfromContaminants
ISNo. TItle
Precautions shall be taken to ensure that the
1070:1992 Reagent grade water (thITd material is free from mercury. copper and
revisions chromiuminanyform; aromaticarnines, aromatic
1699: 1994 Methodsof samplingand lest tor nitro compounds, aromatic hydrocarbons, and
foodcolours(secondrevlSwn) cyanides.
TableLRequirementsfor IndigoCarmine
(Clause3.1)
51 (:harackrisUc RequJremrnt MdhodorTrst,Refto
No. (j\llnc.~-~r:~- Cla~~
Standard IS1699 1994
(1) (2) (3) (4) (~)
I) Toldl dye content.corrected torsample 8~ A
I..Inl'L!.-II10'" -r JUCtor 2-h.percent hy
1ll,/l.~.Mrn
n) l.il"'~ondrymgat IJ-4i"C.percentbyma~", j~ o
"nJ
Chloride and sulphate, expressed tt... 13
sodiumS,ltJr.percentbymass,Max 14
Ill) Waler-Jn!loolublematter,percentbyruass. 04 H
Max
IV) Combrned ether extracts, percent by 0.4 C
mass,Max
v) Subsidiarydyes,percentbyrnass,Max 3.0 D
vs} tsaunsulphonicacrd, percent bymass, E
Max
VI/) Lead,mg/kg.Max 10 15
\'111) Arsenic.rng/kg,Max 3(seeNote] is
IX) Heavymel.Is.mglkg,Max 41l lb
NOTE- Thevalueofarsenich..beenreferredtoCCRiforaoopuonunderthePFARules.
ISl"":~
3.3 Theproductshallbeprocessed,packed,stored 4.2.2 BISCmijicatioilMarking
and distributed under hygienic conditions in TheproductmayalsobemarkedwiththeStandard
licencedpremises(see)S2491:1972). Mark.
4 PACKINGANDMARKING 4.2.2.1 Theuseofthe StandardMarkisgoverned
4.1 Packing bytheProvisionsoftheBureauofIndianStandards
Act, 1986 and the Rules and Regulations made
The materialshall be packed in glasscontainers, thereunder.Thedetailsofconditionsunderwhich
polyethylene container, metal containers, or the licence for theuseof Standard Mark maybe
cardboard containers suilably lined with
grantedtomanufacturersor producersmaybeob
polyethylene. Subject to agreement between the tainedfrom theBureauofIndianStandards.
purchaserand thevendoranyothersuitablecon
tainermayalsobeused. 5 SAMPLING
5.1 Representative samples of the material for
4.2 Martdn&
testsshall be drawn and criteria for ascertaining
4.2.1 Eachcontainershallbelegiblyand indelibly conformitytotherequirementsofIhisspedfication
markedwiththefollowing information: shall be determined according h' the method
prescribedin4ofIS1699: 1994.
a) Thewords 'FOODCOLOVR';
b) Commonnameofthecolour; 6 TESTS
c) Chemicalnameofthecolour;
d) Colourindexnumber; 6.1 Testsshallbecarriedoutasprescnbcdin0014
e) Sourcebfmanufacture; and5ofTable1.
f) Dateofmanufacture;
6.2 Quality01Reagents
g) Netmassingramsor kilograms;
h) BalChorcodenumber;and Unlessspecifiedotherwise,purechemicalsanddis
j) Anyotherrequtrementsasspecifiedunderthe tilledwater(seeIS1070: 1992)shall beemployed
SlondardsofWdghlsandMMSUTes(PodUJ/i,f!d intests.
Commodities} Rules, 1977/Pre"ention of
Non- 'run:CkO:m:ab.'ehallmcanchermcalsthotldunul
FoodAdulrermionRules, 1955. Qmwnempurtneswhichaffecttheresultsofdn.t1)'SI!'o
ANNEX A
[Table 1,Item (i) ]
1)Io:TI-:RMINI\T!ONOilTOTAl.DYI-:CONTI-:NT
04.-0 GENERAL A·l.3 Procedure
Twomethods,spectrophotometricmethodand the Weigh accuratelyabout250mgof the dyesample
titaniumtrichloridemethod,arc specified. Incase anddissolvewithammoniumacetatesolutionina
of dispute, spectrophotometric method shall be 250-mlvolumetricflask;dilute thiswith the same
regardedasthereferencemethod. solventto make afinal concentrationof I mgllOO
mI(approximately).Findouttheopticaldensityof
04.·1 SPE<..'TROPIIOTOMJo.TRlCMJo.TIIOD this dilute solution, against ammonium acetate
04.-1.1 Apparatus solutionasblank,at610nm,inagla~cellwith IIJ.O
mmlight path.
Suitable specrropnotomercr with properly
04.-1.3.1 SimultaneouslyweighaccuratelyaboutZg
calibratedscales for bothwavelength and optical
ofthedye-sampleanddrythisinanair-ovcnat 105
density. However, a suitable spectrocolonmerne :t 1°Cfor 2hours. Calculatethe lo~of mass on
may also be used after calibration against a
<trying;from thisdata,calculatethedrymassufthe
spectrophotometer.
sample(M)inIbetinalsolutiontakenfurmeasure
1\.\.2 Reagents ment ufopticaldensity.
A-1.4 Calculation
04.-1.1.1 AmmonIumAUla/I:Solution
OD x ilK)
ZOOmgofammoniumacetateinonehireutwater Totaldyecontentinthe - M x 4511
sample.percent
(re-distilled).
2
IS1698:1"4
where A-2 TITANIUMTRICIIWRlDEM~"IIOD
OD opticaldensityfound; A·1.1 Proc:edure
M <bymass of the sample in 100 ml
Themethodgiven in 5ofIS 1699 :1994shallbe
solution;and
followed.Thepercentageoftotaldyecontentshall
450 Et~lcm,610nm,forindigocarmine bedeterminedusingthefollowingcalculations:
inammoniumacetatesolution.
1mlof0.1NTiO),.,0.02332gofindigocarmine.
ANNEXB
[Table 1,Item (iii)]
DETERMINATION01<'WATERINSOLUBLEMAlTER
D-} PROCEIHJRF. followedtaking2gramofsampleinsteadof4.5·5g.
The method given in 7otIS1699:1994shallbe (see7.2ofIS1699:1994).
ANNEXC
[Table 1,Item (iv)]
DETERMINATIONorCOMBINEDETHEREXTRACT
C-l PROCEDURE followedtaking2gofsampleinsteadof5g(see8.4
The method given in8 of IS1699:1994shallbe ofIS1699:1994).
ANNEXD
[Table1,Item (v)]
DETERMINATIONOFSUBSIDIARYDYECONTENT
0-1 PROCEDURE NOTES
Themethodgivenin901'IS1699:1994using the 1~5,71S01DCf~SCJlilC1lloJ~~WUJ.~bluezonejustIntrout
conditionsgiven belowshallbefollowed: 0(lbe: mam blue band. De not mctudc atm.Lone 111 the:
M1~LlUlrycolounng matter I.ull(.~whICh arc CUI OUl .tnd
Developingsolvent: No.."l mea:"uroJ.
Heightofascentofsolventfront:approximate 17 :zThe15mlsonumhydrogencarbonatesotunonUM..""dInIhe
em generalprocedure~replacedby15ml0.05Nhy....rochtor«..
acid10order10avoid lhe:decompocuonwhich~UU(H\aIL-d
IDchgoundergoesInalkahnesrnUhOfl
ANNEXE
[Table 1,Item (vi)]
DETERMINATION0" THEISATINSULPffONICACID
E·l PRINCIPLE ";.2.2 Mlcrosyringe - Capableofuehvermg 0.2
mlwith atoleranceof ±11.002ml.
E-l.l Separateisatinsulphonicacidfromindigocar
minebyascendingpaperchromatographyandextract E·2.3 Spectrophotometer
itfrom thepaper.Measuretheopticaldensityofthe
E-3 RF.AGJo;NTS
extraet at the wavelength ofmaximum absorbance
around247nm,from whichcalculatethecontentof E-3.1 AtmosphereSaturatingSolvent- Water.
theintermediateasapercentbymassofthesample. E·3.2 Developing Solvent - SOdium cntonde
E-2 APPARATUS solution15percent.
E·2.1 ChromatographTankwithAncillaryEquip E-3.3 ExtractingSolvent_. Ammoniumsulphate
ment-k given under7.2.1oflS1699: 1974. solution,25percen\.
IS16911:1994
E-4 PROCEDURE E-4.2 Holding the chrol1latogram under
ultravioletlight,markIhetopandthebottomofthe
1<:-4.1 Markoutasheetofchromatographypaper fluorescentband. (Rfvalue ofIsaun-o-sulphonic
a.'shownInFIg.2ofIS1699:1994.Apply0.2m!of acidis0.9). CuI the bandfrom Ihesheetasastrip
a 1.0 percent m]v (based on lUO percent purity) and CUI an equivalentstrip from the plain sheer,
aqucoussolutionofthedyeasuniformlyaspossible Extract eaeh srnp separately with 10ml of the
within the confinesofthe 180 mm x 7mm rec ammonium-sulphatesolution and filter. Measure
langle. holding the nozzle of the micro-syringe theopticaldensityofIheextractsat thepeaknear
stcadrlyincontactwith thepaper.Allow thepaper 247:!:5nm,
10dryat room temperaturefor I 102hours,orat
:'ll"Cfor:'minutesfollowedby15minutesatroom E-S CALCULAnON
temperature. Mount the sheet, together with a E-S.l Isatinsuiphonicacid, =(D-d) x F
plain sheet to act as a blank. in frame (C). Pour percentbymass
sulucientdevelopingsolvent into the tray (D) 10
where.
bnngthesurfaceof Ihe solvent about 1embelow
the hase line of the chromatogramsheet. PUIthe D theopticaldensityofthe isatinsul
trame(C)inposnionandreplaceIhecover.Allow phonicacidextract,
Ihe"ilventtoascentIIIthefullheighIofthepapers. d the optical densitv of the cor
Irunxtcrthe paper,to adryingcabmei and dryat respondingblank. and
'0(,(/"C.Remove (heshect«from trame(C). F 6.0.

4