IS 15614: Methomyl, Technical

2006·1 MB·English

by  Bureau of Indian Standards

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IS 15614 (2006): Methomyl, Technical [FAD 1: Pesticides and
Pesticides Residue Analysis]
“!ान $ एक न’ भारत का +नम-ण”
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IS 15614:2006
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Indian Standard
METHOMYL, TECHNICAL — SPECIFICATION
ICS65.100.10

0 BLS2006 .
BUREAU OF INDIAN STANDARDS
MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARG
NEW DELHI 110002

February 2006 Price Group 2
Pesticides and Pesticides Residue Analysis Sectional Committee, FAD 1
FOREWORD
This Indian Standard was adopted by the Bureau of Indian Standards, afier the draft finalized by the Pesticides
and Pesticides Residue Analysis Sectional Committee had been approved bythe Food and Agriculture Division
Cou,ncil.
Methomyl, technical isemployed inthepreparation of insecticidal formulations foragricultural use.
Methomyl isthe accepted common name bythe International Organization for Standardization (1S0) forMethyl
N-[{(methylamino) carbonyl} oxy ]=ethanimidothioate
The empirical and structural formulae andthemolecular mass ofmethomyl aregiven below:
Empirical Formula Structural Formula Molecular Mass
C~HION20ZS 162.2
SCH3
/
CH3NHC0,N =C
\ CH
3
Inpreparation of this standard, due consideration has been given to theprovisions ofthe Insecticides Act, 1968
andtheRulesframedthereunder. However,thisstandard issubjecttotherestrictions imposedundertheInsecticides I
Act and Rules, wherever applicable.
For the purpose of deciding whether a particular requirement of this standard iscomplied with the final value,
observed or calculated, expressing the result of a test or analysis, shall be rounded off in accordance with
IS2:1960 ‘Rules for rounding off numerical values (revised)’. The number ofsignificant places retained inthe
rounded offvalueshould bethe same asthat ofthe specified value inthis standard.
I
IS 15614:2006
Indian Standard
METHOMYL, TECHNICAL — SPECIFICATION
1 SCOPE covered with steel cover with flown-in rubber gasket
and galvanized steelring.
This standard prescribes the requirements and
the methods of sampling and test for MethomyI, The container shall also conform to the general
technical. requirements given inIS 8190 (Part 1).
2 REFERENCES 5 MARKING I
The fol!owing standards contain provisions which 5.1 Thecontainers shallbear legibly and indelibly the
through reference inthis text, constitute provisions of following information in addition to the information
this standard. At the time of publication, the editions as is necessary under the Insecticides Act, 1968 and
indicated were valid. All standards are subject to Rules framed thereunder:
revision, and parties to agreements based on this
a) Name ofthe material;
standard are encouraged to investigate the possibility
of applying the most recent editions of the standards b) Name and address ofthemanufacturer;
indicated below: c) Batchnumber;
d) Date ofmanufacture;
1SNo. Title
e) Date ofexpiry;
1070:1992 Reagent gradewater (third revision)
f) Net mass of contents;
6940:1982 Methods of test for pesticides and
their formulations @-st revision) g) Nominal methomyl content, percent (rn/rn);
h) The cautionary notice as worded in
8190 Requirements for packing of
Insecticides Act, 1968 and Rules framed
(Part 1): 1988 pesticides: Part 1 Solid pesticides
thereunder; and
(second revision)
j) Any other information required under the
10946:.1996 Methods for sampling for technical
Standards of Weights and A4easurex
grade pesticides.first revision)
(Packaged Commodities) Rules, 1977.
3 REQUIREMENTS
5.2 BIS Certification Marking
3.1 Description
‘Theproduct may also be marked with the Standard

The material shall beinthe form of off-white powder, Mark.

free from extraneous impurities.
5.2.1 The use of the Standard Mark is governed by
3.2 The material shall also comply withrequirements the provisions ofthe Bureau of Indian Standards Act,
given inTable 1. 1986andtheRules andRegulations made thereunder.
ThedetailsofconditionsunderwhichtheIicencefor the
4 PACKING
useof StandardMarkmaybe grantedtomanufacturers
The material shall be packed in 50 kg fibre drums orproducersmaybe obtainedfromtheBureauofIndian
having suitable inner packing. The drums would be Standards.
Table 1Requirements for Methomyl, Technical
(Clause 3.2)
SI Characteristic Requirement Method ofTest, Refto
No. A
/ Y
Annexofthis ClauseNo.of
Standard IS6940
(1) (2) (3) (4) (5)
i) Methomyl content,percentbymass,&fin 98.0 A —
ii) Moisture content,percentbymass,Max 0.5 — 4

1
I
IS 15614:2006
6 SAMPLING 7.2 Quality of Reagents
Representative samples shall be drawn as prescribed Unless specified otherwise, pure chemicals and
inIS 1Q946. reagent grade water (see IS 1070) shall be employed
>
intests.
7 TESTS
7.1 Tests shall becarried out bythe methods referred NOTE — ‘Purechemicals’ shall mean chemicals that do not
to inCO14 and 5of Tablel. containimpurities, whichaffecttheresoits ofanalysis,
ANNEX A
[Table 1, Item (i)]
DETERMINATION OF METHOMYL CONTENT
A-1 PR-INCIPLE A-3.2 Water, HPLC/AR grade.
Methomyl content isdetermined byhigh performance A-3.3 Methanol, HPLC/AR grade.
liquidchromatography (HPLC) using internalstandard
A-3.4 Benzamide, internal standard, ofknown_purity.
technique.
A-3.5 Methomyl, analytical standard, of known
A-2 APPARATUS
purity.
A-2.1 A high performance liquid chromatography
&3.6 Phosphoric Acid, IWLCIARgrade, 85percent.
system coupled with ultraviolet detector andaprinter-
plotter-cum-integrator isused for this determination. A-4 PROCEDURE
The suggested operative parameters areasfollows, but
A-4.1 Preparation of Internal Standard
canbe changed ifnecessary, provided standardization
isdone. Weigh 16gof benzamide into a 1000 ml volumetric
flask. Add 200ml of methanol inthe flask to dissolve
Column Reverse phase C8, C18 or
equivalent pre-packed 5-pm benzamide completely and make up to volume with
particle size column, 150mm methanol.
x 4.6 mm, with a 0.5 pm in-
A-4.2 Preparation of Mobile Phase and Sample
Iine pre-column filter with
Solvent System
replaceable 0.5 ~m frit (filter

required to protect the Weigh 1gof85percent phosphoric acid ina2000 mI

analytical column) volumetric flask and make up to volume with HPLC
Temperature Ambient grade water. This solution isa0.0425 percent H~PO~
Mobile phase Acetonitrile:Water(10:90,v/v), aqueous solution with apH ofapproximately 2.7.
pH of water = 2.7 adjusted
with 85 percent phosphoric A-4.3 Preparation of Standard Solution
acid (H3P0,) Weigh accurately 100 mg of methomyl analytical
Flow rate 1.5ml/min standard andadditintoa100mlvolumetric flask. Add
Injection volume : 20 microlitre 4mlofbenzamide internal standard solution. Diluteto
Detector Ultraviolet detector (254 nm) volume with water ofpH 2.7 and label the bottle as
methomyl standardsolution.Dissolveultrasonicallyyfor
A-2.2 Analytical Balance
5rein,filterthestandardsolutionthrough 0.2pm filter,
A-2.3 Microiitre Syringe — 25 microlitre. and inject standard induplicate.
A-2.4 Filter Units, 0.2 ~m pore size. A-4.4 Preparation of Sample Solution
A-2.5 Ultrasonic Bath
Weigh accurately 100mg of methomyl, technical ina
A-2.6 Standard Glassware 100ml volumetric flask. Add 4 ml of the benzamide
internalstandardsolution intheflask. Dilutetovolulne
A-3 REAGENTS
withpH 2.7 water (0.042 5percent H~POiwater) and
A-3. 1 Acetonitrile, HPLC/AR grade. ultrasonicate for 5 min. Label the flask as sample
2
IS 15614:2006
working solution. Filter through a 0.2 ~m filter and A-5 CALCULATION
analyze induplicate.
M,x A,x A,x P
A-4.5 Estimation Methomyl content, percent rrim = “ “ “
M2x A4x AI
A-4.5.1 Filter a portion of all sample and standard
solutions through a0.45 pm filterunit before analysis. where
Afterthecolumn hasequilibrated andastablebaseline ~, = mass of methomyl in standard solution,
isobtained. inject, in duplicate, 20 microlitre each of
ing;
standard and sample solution.
&f2 . massofthe sample taken for test, ing;
A-4.5.2 Retenlion Time (Guide Values) A, = peakareaofmethomyl inthechromatogram
of standard solution;
Methomyl : 6.0 min
A2 = peak area of standard methomyl in the
Benzamide : 5.5min
chromatogram ofsample solution;
(Internal standard)
A, = peakareaofinternal standard, benzamide,
Total run time : 7min inthe chromatogram of-standard solution;
A-4.5.3 From the chromatograms of the standard A, = peakareaofinternal standard, benzamide,
solution and sample solution measure the peak areas in the chromatogram of sample solution;
of the active ingredient and the internal standard and and
calculatethepercentage oftheactiveingredientasgiven P= percentagepurityofmethomyl inreference
inthe calculation (see A-5). standard.

3
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needed; if the review indicates that changes are needed, it istaken up for revision. Users of Indian Standards
should asce~in that they are inpossession ofthe latest amendments or edition by referring tothe latest issue of
‘BIS Catalogue’ and ‘Standards :Monthly Additions’.
This Indian Standard has been developed from Doc :No. FAD 1(1346).
Amendments Issued Since Publication
Amend No. Date of Issue Text Affected
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