IS 15600: Cymoxanil , Technical

2005·1 MB·English

by  Bureau of Indian Standards

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IS 15600 (2005): Cymoxanil , Technical [FAD 1: Pesticides
and Pesticides Residue Analysis]
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IS 15600:2005
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Indian Standard
CYMOXANIL, TECHNICAL — SPECIFICATION
ICS65.100.30
. . ..

0 BIS2005
BUREAU O-F INDIAN STANDARDS
MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARG

NEW DELHI 110002
November 2005 Price Group 2
..
Pesticiclesand Pesticides -ResidueAnalysis Sectional Committee, FAD I
FOREWORD
This Indian Standard was adopted by the Bureau of Indian Standards, after the draft finalized by the Pesticides
and Pesticides Residue Analysis Sectional Committee had been approved bythe Food and Agriculture Division
Council.
Cymoxanil, technical isemployed inthe preparation offungicidal formulations for agricultural use.
Cymoxanil is the accepted common name by the International Organization for Standardization ( 1S0 ) for
~-cyano-N-[ (ethylamino )carbonyl ]-2-( methoxyimino )acetamide.
The empirical and structural formulae and the molecular massofcymoxanil aregiven below.
Empirical Formula Structural Formula Molecular Mass
C7H10N403 HI 198.18
N=C-C-C-NH-C-NH-CHZCH3
II
NOCH3
In preparation of this standard, due consideration has been given to the provisions of the Insecticides Ac(,
1968 and the Rules framed thereunder. However, this standard is subject to the restrictions imposed under the
Insecticides Act, 1968 and Rules, wherever applicable.
For the purpose of deciding whether a particular requirement of this standard is complied with the final
value, observed or calculated, expressing the result of a test or analysis, shall be rounded off in accordance
with 1S2: 1960 ‘Rules for rounding off numerical values (revised)’. The number of significant places retained
inthe rounded off value should be the same.as that of the specified value inthis standard.
IS 15600:2005
Indian Standard
CYMOXANIL, TECHNICAL — SPECIFICATION
1 SCOPE 4 PACKING
This standard prescribes the requirements and The product shall be packed”in multi-layered bag
the methods of sampling and test for cymoxanil, of capacity 20 kg with suitable leak proof and
technical. pilfer proof closure system. The container shall
also conform tothe general requirements asgiven.in
2 REFERENCES
ISt3190(Part 1).
The following standards contain provisions which
5 MARKING
through reference inthis text, corrstitute provisions
of this standard. At the time of publication, the 5.1 The containers shall bear legibly and indelibly
editions indicated were valid. All standards are the folIowing information in addition to the
subject to revision and parties to agreements based information as is necessary under the Insecticides
on this standard are encouraged to investigate the Act, 1968 and Rules framed thereunder:
possibility ofapplying the mostrecent editions ofthe
standards indicated below: a) Name ofthematerial;
b) Name and address of the manufacturer;
IS No. Tit[e
c) Batch number;
1070:1992 Reagent grade water ( third
d) Date of manufacture;
revision )
e) Dateofexpiry;
6940:1982 Methods of test for pesticides and
f) Net mass of contents;
their formulations (jht revision)
13) Nominal cymoxanil content, percent (m/m);
8190 Requirements for packing of
h) Cautionary notice asworded inInsecticides
(Part I):”1988 pesticides: Part 1Solid pesticides
Act, 1968 and Rules framed thereunder; and
(second revtiion )
.0 Any other information required under Ihe
10946:1996 Methods of sampling for technical
Standards of Weights ‘and Measures
grade pesticicles(first revision )
(Packaged Commodities) Rules,.1 977.
3 REQUIREMENTS 5.2 BXSCertification Marking

3.1 Description
The product may also be marked with the Standard
The material shall be inthe form of peach coloured Mark.
homogeneous powder,freetlom extraneous impurities
5.2.1 The use of the Standard Mark isgoverned by
and hard lumps.
the provisions of the Bureau of Indian Standards
3.2 Thematerial shallalsocomply withrequirements Act, 1986 and the Rules and Regulations made
given inTable 1. thereunder. The details of conditions under which
Table1 Requirements forCymoxanil, Technical
(Clause 3.2)
S1 No. Characteristic Requirement Method of Test, Ref to
Annex of Clause No. of
thisStandard IS 6940
(1) (2) (3) (4) (5)
i) Cymoxanil content, percent by mass, &fin 96.0 A —
ii) Material insoluble in acetone, percent by mass, Mm 0.1 — 9
iii) Moisture content, percent by mass, Mox 0.2 — 4.2
iv) Acidity ( asH2S04 ), percent by mass,Max 0.2 — 11.3.2

1
Is 15600:2005
the-Iicencefor the use of the Standard Mark maybe to inCOI4and 5of Table 1.
granted to manufacturers or producers may be
7.2 Quality ofReagents
obtainedfiom the Bureau of Indian Standards.
6 SAMPLING Unless specified otherwise, pure chemicals and
reagent grade water (see IS 1070) shall beemployed
Representative samples ofthematerial shallbedrawn
in tests.
asprescribed inIS 10946.
NOTE — ‘Pure chemicals’ shall mean chemicals that
7 TESTS
do not contain impurities, which affect the results of
7.1 Testsshalj becarried out bythemethods referred analysis.
ANNEX A
( Table 1)
DETERMINATION OF CYMOXANIL CONTENT
A-1 PRINCIPLE A-2.5 Ultrasonic Bath
Cymoxanil content is determined by high A-2.+ Standard Glassware
performance liquid chromatography (HPLC )using
A-3 REAGENTS
internal standard technique.
A-3.1 Acetonitrile, HPLC grade.
.A-2 APPARATUS
A-3.2 HPLC GradeWater
A-2.1 High Performance Liquid Chramatograph,
equipped with a constant flow pump, -constant A-3.3 Acetanilide, ( internal standard ] of known
temperature column compartment, sample injector purity.
capabjeofinjecting25plaliquots, a254mwavelength
A-3.4 Cymoxanil, analytical standard of known
ultraviolet detector.artd arecorder (digital integrator
purity.
or other data handjing device ) is used for this
determination. The suggested operative parameters A-3.5 Ortho Phosphoric Acid, HPLC grade,

are as follows, but can be changed if necessary, 85percent.

provided standardization isdone:
A-4 PROCEDURE
Column Reverse phase C8, C18 or
A-4.1 Preparation ofMobile Phase
equivalent pre-packed column,
4.6x 250mm Adjust thepH of water to 2.8 with ortho phosphoric
acid. Add 1500 ml of thepH 2.8 adjusted water to
Temperature Ambient
500 ml of acetonitrile in a flask , mix thoroughly.
Filter the mixture using the fikering apparatus.
Mobile phase Acetonitrile : Water ( 2“5:75,
v/v),pH of water= 2.8 adjusted
A-4.2 Preparation ofInternalStandard
with 85 percent phosphoric acid
Weigh accurately 400 mg of acetanilide into a
(H~pOq)
100mlvolumetricflask. Makeup tovolumewithHPLC
Flow rate 1.5ml/min grade acetonitrile and ultrasonicate for 5min.
Injection vojume : 20pl A-4.3 Preparation ofStandard Solution
Detector : Ultraviolet detector (254 nm) Weigh accurately 35 mg of cymoxanil analytical
standard of known purity and add it into a 25 ml
A-2.2 Analytical Balance
volumetric flask. Pipette 5mlofacetanilide internal
standard solution into the volumetric flask. Make
A-2.3 Microlitre Syringe —25 pl.
up to volume with acetonitrile and mix. Dissolve
A-2.4 -FilteringApparatus forSampleandStandard ultrasonically for 5 min and filter the standard
Solutions solution through 0.45 pm filter.
2
IS 15600:2005
A-4.4 Preparation ofSample Solution given inthe Calculation (see A-5).
Weigh accurately 35 mgof cymoxanil,-technical and A-5 CALCULATION
add it into a25-ml volumetric flask. Pipette 5 ml of
MIXA3XAZXP
internal standard solution in the flask. Make up to
Cymoxanil content, percent m/m = M ~A x~
volume with acetonitrile, mix and ultrasonicate for
24i
5min. Filter the solution through the0.45 pm filter.
where
A-4.5 Estimation M, = mass of cymoxanil in standard solution,
A-4.5.1 Equilibrate the column with the mobile ing;
phasefor45minbefore injectingthestandard/sample. ~2 . mass of the sample taken for test, ing;
After the column has equilibrated and a stable
A, = peakareaofcymoxanilinthechromatogram
baseline isobtained, inject. induplicate, 20 p] each
of standard solution;
of standard and sample solution.
peak area of-standard cymoxanil in the
A-4.5.2 Retention Time chromatogram ofsample solution;
Cymoxanil : 13.Omin peak area of internal standard,
acetanilide, in the chromatogram of
Acetanilide ( Internal standard ) : 8.4 min
standard solution;
Total run time : 15.Omin peakareaofinternal standard, acetanilide,
inthe chromatogram of sample solution;
A-4.5.3 From the chromatograms of the standard
and
solution and sample solution measure the peak areas
ofthe active ingredient and the internal standard and P= percentagepurityofcymoxanil inanalytical
standard.
calculate the percentage of the active ingredient as
.

3
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of ‘BIS Catalogue’ and ‘Standards : Monthly Additions’.
This Indian Standard has been developed from Doc :No. FAD1( 1534).
Amendments Issued Since Publication
Amend No. Date of Issue Text Affected
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