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इंटरनेट मानक Disclosure to Promote the Right To Information Whereas the Parliament of India has set out to provide a practical regime of right to information for citizens to secure access to information under the control of public authorities, in order to promote transparency and accountability in the working of every public authority, and whereas the attached publication of the Bureau of Indian Standards is of particular interest to the public, particularly disadvantaged communities and those engaged in the pursuit of education and knowledge, the attached public safety standard is made available to promote the timely dissemination of this information in an accurate manner to the public. “जान1 का अ+धकार, जी1 का अ+धकार” “प0रा1 को छोड न’ 5 तरफ” Mazdoor Kisan Shakti Sangathan Jawaharlal Nehru “The Right to Information, The Right to Live” “Step Out From the Old to the New” IS 14833 (2000): Lindane Wettable Powder [FAD 1: Pesticides and Pesticides Residue Analysis] “!ान $ एक न’ भारत का +नम-ण” Satyanarayan Gangaram Pitroda ““IInnvveenntt aa NNeeww IInnddiiaa UUssiinngg KKnnoowwlleeddggee”” “!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता हहहहै””ै” Bhartṛhari—Nītiśatakam “Knowledge is such a treasure which cannot be stolen” IS 14833:2000 *m Indian Standard LINDANE WETTABLE POWDER — SPECIFICATION ICS 19.020; 65.100.01 r 0 BIS 2000 BUREAU OF INDIAN STANDARDS MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARG NEW DELHI 110002 October 2000 PriceGroup 2 Pesticides Sectional Committee, FAD 1 FOREWORD This Indian Standard was adopted by the Bureau of Indian Standards, after the draft finalized by the Pesticides Sectional Committee had been approved by the Food and Agriculture Division Council. This formulation is based on Lindane ( CAS No. 58 -89-9 )a broad spectrum, pesticide. (Gamma Isomer of -HCN having minimum purity of 99.5 percent almost free from Alpha, Beta, Delta, etc, isomers is called Linckme). Lindane wettable powders are generally manufactured to contain 6.5 percent Lindane (m/m.) as active ingredient. Lindane wettable powders are widely used for control of large number of pests in agriculture, animal and.public health at low rate of application. In the preparation of this standard, due consideration has been given to the provisions of the Insecticides Act, 1968 and the Rules framed thereunder. However, this standard is subject to the restrictions imposed under these, wherever applicable. For the purpose of’deciding whether aparticular requirement of this standard iscomplied-with, the final value, observed or calculated, expressing the result of a test or analysis, shall be rounded off in accordance with IS 2:1960 ‘Rules t’orrounding off numerical values (revised)’. The number of significant places retained inthe rxmnded oft’value should be the same as that of the specified value in this standard. IS 14833:2000 . Indian Standard LINDANE WETTABLE POWDER — SPECIFICATION 1 SCOPE declared nominal value by more than the tolerance limits indicated below: This standard prescribes the requirement and the method of sampling and tests for Lindane wettable Nonlinal Vulue,Percent Tolerance Limit powders. up to 9 +Iq 2 REFERENCES -5 I The Indian “Standards listed below are necessary 10and below 50 *5 Ofthenominal value adjuncts to this standard: 50 and above +5 -3J IS No. Title 460 (Part 1) : Specification for test sieves : 4 PACKAGING AND MARKING 1985 Part 1wire cloth test sieves (third 4.1 Packaging revision) The material shall be packed in mild steel or tin plate 882:1984 Specifications for Lindane ~irst or fibre board containers lined with polyethylene of revision) thickness not less than 0.062 mm. Retail pack up to 1070:1992 Reagent grade water (third revision) 1kg of material shall be packed in polyethylene bag 6604:1991 Code for packaging ot’solid pesti- of not less than 0.062 mm thickness, its mouth heat- cides (up to 1000 g) (first revision) sea]ed and then packed in cardboard carton 6940 (Part I) : Method oftest for pesticides: Part 1 individually. Carton for packs up to 500 g shall be as 1982 Solid pesticides (second revision) given in IS 6604 but carton for 1kg packs shall be an ‘E’ fluted corrugated board carton of minimum 8190 (Part 1) : Requirement for packing of pesti- specification 240 gsm duplex board/80 gsm kraft. The 1988 cides :Part 1Solid pesticides general requirement of packaging shall conform to 2 10627:1983 Method for sampling of pesticidal of IS X190 (Part 1)and amendment thereof, if any. formulations 4.2 Marking 3 REQUIREMENTS 3.1 Constituents The container shall bear legibly and indelibly the following information in addition to the information 3.1.1 The material shall consist of Lindane together as is necessary under the Insecticides Act, 1968 and with suitable carrier(s), stabilizers(s) and other Rules framed thereunder: formulant(s). a) Name of the material; 3.1.2 Lindane employed in the manufacture of the material shall conform to IS 882 with up to date b) Name of the manufacturer; amendments. c) Date of manufacture and date of expiry; 3.2 Description d) Batch number; The material shall be afine, free flowing white to off e) Net mass of contents; white homogeneous powder, free from visible f) Nominal value of Lindane content, percent extraneous matter and hard aggregates. It shall wet (n/\Jn); and readily on mixing with water providing a suspension g) The cautionary notice worded as in Insecticides suitable for use as spray. Act, 1968 and Rllk. 3.3 The material shall also comply with the 4.2.1 BIS Certijicution Marking requirements given in Table 1. The product may also be marked with the Standard 3.4 Lindane Content Mark. When determined by the method prescribed in Annex A, the observed Lindane content, percent by 4.2.1.1 The use of the Standard Mark is governed by mass of any of the samples shall not differ from the theprovisions of the Bureau of Indian Standards Act, 1 IS 14833:2000 Table 1 Requirements “forLindane Wettable Powders (Clause 3.3) S1No. Characteristic Requirement ‘Method ofTest 1Ref to I Annex Cl No. ofthis of Standard IS 6940 (1) (2) (3) (4) (5) O Lindane cootent, percent by mass Nominal value asdeclared — onthe container (See 3.4) A ii) Material passing through 97.0 11.1 75 micron IS sieve, osper 1S460 (Part 1), percent by mass, &fin iii) Suspensibility, after accelerated 50.0 11.2 storage, percent by mass, &fin($eeNote) iv) WettaMity inseconds, Mux 120 — 11.4 v) Acidity (as HZS04), percent by mass. 0.251 Max ORAIkafinity (as NaOH), 11.3 percent by mass, Mox 1,0 vi) Related isomers Topass test B NOTE — The material need notbesubjected toaccelerated storage treatment ifithas crossed half ofitsshelf lifeosascertained from its date of manufacture and expiry declared on the container. 1986 and the Rules and Regulations made thereunder. 6.2.2 After conducting the suspensibility test as The derails of conditions under which the licence for described in IS 6940 shake vigorously the remaining the use of Standard Mark may be granted to one tenth suspension including sediment with manufacturers or producers maybe obtained from the sufficient acetone. Make the volume to 250 ml with Bureau of Indian Standards. acetone. 5 SAMPLING 6.2.3 Transfer the lfJml ofsolution-quantitatively to a 250 ml separating funnel. Add 100 ml of 2 percent When a bulk quantity of the material is offered for Sodium Chloride solution. Extract theLindane with 3 inspection, representative samples of the material portions each of 50 ml rz-hexane. Filter the total shall be drawn as prescribed in IS 10627 and tested n-hexane layer through a bed of anhydrous sodium within 90 days of its manufacturing. The criteria sulphate. Wash Sodium Sulphate twice with 10-15 ml for conformity shall be as given in IS 10627. When n-hexane. Evaporate the n-hexane on awater bath and the material “isoffered for inspection after 90 days dissolve the residue in 5 ml acetone (taken by pipette) of its manufacturing sampling shall be done and add to it 5ml internaI standard solution by pipette according to IS 10627 and the criteria for (see A-1.4.1). Shake well to homogenize. Use this conformity shall be as given in IS 10627. However, solution for injection in gas chromatographic column the criteria for conformity of the material when as described in A-1.2.1, tested shall be the limit of tolerances, as applicable over the declared nominal value given under 3.4 of Lindane contenting in the suspension including this standard. M,x R2x PxD 6 TESTS sediment = m = R, X 100 6.1 Test shall be carried out by the methods referred where to in CO14 and 5 of Table 1and 6.2. M, = Mass in .gof Lindane in reference 6.2 Suspensibility Test standard solution, RI = Response factor for the Lindane 6.2.1 For carrying out suspensibility test start with reference standard solution, 0.5 percent Lindane tiv concentration after accelerated storage at 54 ~ l“C for 24 hours and proceed as R, = Response factor for the sample solution, described in 11.2 of IS 6940. injected, 2 IS 14833:2000 P = Percent purity of Lindane reference m= Mass ingof Lindane present in the standard, and suspension including the sediment. D = Dilution factor = 5 %.3 QuaIity-of Reagents Suspensibility, looo(rvf-tn) Unless specified otherwise, pure chemicals and percent by tnass = 9A4 distilled water (see IS 1070) shall be employed in where tests. M = Mass, in g of Lindane present in the NOTE — ‘Pure chemicots’ shatl mean chemicats thatdo not sample taken for the preparation of the contain impurities wtich affect the results ofsmatysis. suspension; and ANNEX A [Table 1,S2No. (i)] METHOD FOR DETERMINATION OF LINDANE CONTENT IN LINDANE WETTABLE POWDER A-OGeneral A-1.3.2 Lindane standard of known purity. Lindane content is determined by gas liquid A-1.3.3 Internal Standard chromatography by using an internal standard and a Alpha naphthol. flame ionization detector. A-1.4 Preparation of Standard and Sample A-1 GAS CHROMATOGRAPHIC METHOD Solutions A-1.l Gas liquid chromatography fitted with a flame ionization detector (FID) is used for lindane content A-1.4.1 Preparation of Internal Standard Solution determination. Using -solution containing known Weigh accurately about 150 mg of internal standard amounts of the standard Lindane and internal standard into a 100 ml volume with glass stopper. Add by the response factor R for Lindane content and internal pipette 10ml of internal standard solution and 10ml standard is arrived at a solu(ion containing a known acetone. mass of sample and internal standard is injected into Stopper the flask, tightly and gently swirl the theGLC unit. The percentage of Lindane content is contents for one minute using a rotational motion then computed. of the wrist. Allow to stand for at least five minutes and then shake for one minute. Centrifuge the above A-1.2 Apparatus solution in a stoppered tube and use supernatant A-1.2.1 Gas Liquid Ck-omatogruph layer as sample solution for injection in gas The instrument should be one that is designed for use chromatographic column. with glass columns and that is equipped with an on- A-1.5 Estimation column injection system and a high-sensitivity flame ionization detector. The suggested parameters aregiven A-1.5.1 operating Conditions in the operating conditions (A-1.5.1) which can be Operating conditions forgasliquid chromatography: The changed depending upon the available equipment and temperatures, gas flow rates and retention times given allied facilities provided standardization is done. below are typical values and may have to be adjusted to Chromatographic column: The column should be a obtain optimum results from agiven apparatus. borosilicate glass tube 183cm length and 4mm internal Gas Flow dia. packed with 5 percent OV-210 on chromosorb Carrier Gas (Nitrogen) 25 ml/min W-HP (100- 120 mesh) or suitable equivalent, bent to Fuel Gas (Hydrogen 30 ml/min fit the chromatography. Air 300 ml/min A-1.2.2 Microsyringe Tempemture 10micro litre capacity Column Oven 160’C Injection Port 220’C A-1.3 Reagents .Detector 240”C A-1.3.1 Acetone Chart Speed 0.5 cm/min Analytical reagent grade. Injection Size 2micro Iitre 3 IS 14833:2000 Typical Retention Times: Linclane Gamma HCN content, Alpha-naphthol (1.S.) peak 2.7 min R xW’XP percent by mass = Lindane peak 6.6 min R’XW X 2.5 A-1.5.2 Inject 2.0 (1 portion of standard Lindane where solution until response factor (peak area) for Lindane R = Response factor for sample to internal standard agrees to within two percen(. solution, A-1.5.3 Inject duplicate aliquots ofthe sample solution R’ = Response factor for standard solution, using same column as that of standard. Me:~sure the response factors and compute Lindane content. w = Weight of sample, W‘ = Weight of Lindane standard, and .— A.lxi CALCULATIONS P = Percent purity ofLindane standard. ,,I Area of Lindane Peak Response Factor (R) = NOTE—Atypical chromatogmm fortheinternat standard and Area ofInternal Standard Peak st:mdiml LIndime solution isshown inFig. 1, 0.44 F .73 6.595 STOP cow NAME TIME $lftEA 96.-7691 11,44 2166$71 3.5733 2.73 12580 0.6372 6,595 -------- 13986- -----.- ------ 100 TOTAL 2193439 ---- FIG. 1CHROMATOGRAMOFLINDANESTANDARD ANNEX B [Table 1S1-NO.(vi)] DETERMINATION OF RELATED ISOMERS IN LINDANE DUSTING POWDER B-O GENERAL are any secondary peaks corresponding to alpha, beta or delta isomers and area of any such peak is more For the checking of presence of other isomers than than one percent of the area of lindane peak. lindane, gas chromatogram obtained inAnnex A is to be examined. Typicul Retention Time -B-1 GLC METHOD Alpha-naphthol (1.S.) peak 2.7 min GLC chromatogmrn obtained during determinz~[ion of Alpha-isomer peak 5.5 min Iindane (Annex A) should normally show peak Lindane peak 6.6 min corresponding to internal standard and lindane only. Beta-isomer peak 8.1 min Product does not conform to the specification it’there Delta-isomer peak 9,0 min -4 Bureau of Indirm Standards BIS is a statutory institution established under the Bureau of Indian Standards Act, 1986 to promote harmonious development of the activities of standardization, marking and quality certification of goods and attending to connected matters in the country. Copyright BIS has the copyright of all its publications. No part of these publications may be reproduced in any form w“ithout the prior permission in writing of BIS. This does not preclude the free use, in the course of implementing the standard, of necessary details, such as symbols and sizes, type or grade .designations. 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