IS 13829: Pesticide - Method for Determination of Residues in Agricultural and Food Commodities, Soil and Water - Atrazine and Simazine

10 Pages·1993·1.2 MB·English

by  Bureau of Indian Standards

इंटरनेट मानक
Disclosure to Promote the Right To Information
Whereas the Parliament of India has set out to provide a practical regime of right to
information for citizens to secure access to information under the control of public authorities,
in order to promote transparency and accountability in the working of every public authority,
and whereas the attached publication of the Bureau of Indian Standards is of particular interest
to the public, particularly disadvantaged communities and those engaged in the pursuit of
education and knowledge, the attached public safety standard is made available to promote the
timely dissemination of this information in an accurate manner to the public.
“जान1 का अ+धकार, जी1 का अ+धकार” “प0रा1 को छोड न’ 5 तरफ”
Mazdoor Kisan Shakti Sangathan Jawaharlal Nehru
“The Right to Information, The Right to Live” “Step Out From the Old to the New”
IS 13829 (1993): Pesticide - Method for Determination of
Residues in Agricultural and Food Commodities, Soil and
Water - Atrazine and Simazine [FAD 1: Pesticides and
Pesticides Residue Analysis]
“!ान $ एक न’ भारत का +नम-ण”
Satyanarayan Gangaram Pitroda
““IInnvveenntt aa NNeeww IInnddiiaa UUssiinngg KKnnoowwlleeddggee””
“!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता हहहहै””ै”
Bhartṛhari—Nītiśatakam
“Knowledge is such a treasure which cannot be stolen”

SI 13829 : 3 399

Indian Standard
PESTICIDE - DOHTEM ROF NOITANIMRETED
FO SEUDISER NI LARUTLUCIRGA DNA DOOF
,SEITIDOMMOC LIOS DNA RETAW -
ENIZARTA DNA ENIZAMIS
UDC 664 : 543 [ 632’95’028 ATR ] +

@ BIS 1993
BUREAU OF INDIAN STANDARDS
MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARC
NEW DELHI 110002
October 1993 Price Group 2
sedicitseP eudiseR sisylanA lanoitceS ,eettimmoC FAD 34 .
FOREWORD
sihT naidnI dradnatS was detpoda yb eht uaeruB fo naidnI Standards retfa eht tfard dezilanif yb
eht sedicitseP eudiseR sisylanA lanoitceS eettimmoC had neeb devorppa yb eht dooF and erutlucirgA
noisiviD .licnuoC
,htoB enizarta ( enizairt-5-onimalyporposi-6-onimalyhte-4-orolhc-2 ) and enizamis ( ,4-orolhc-2
enizairt-s-onimalyhte-sib-6 ) era desu as sedicibreh ni erutlucirga rof eht lortnoc fo .sdeew
Assessment fo rieht seudiser ni doof seitidommoc is erofereht an tnatropmi pets ni gnidraugefas
human htlaeh and tnemhsilbatse fo yrotaluger .ycilop
sihT standard lliw elbane eht htlaeh seitirohtua and srehto degagne ni eht dleif ot wollof mrofinu
tset serudecorp rof eht noitamitse fo seudiser fo enizarta and enizamis ni suoirav .seitidommoc
nI eht noitaraperp fo this standard, eud noitaredisnoc has neeb nevig ot eht mumixam stimil fo
enizarta and enizamis seudiser laid rednu eht snoisivorp fo
Prevention of Food Adulteration Act,
and eht seluR demarf .rednuereht ehT tset dohtem is detcirtser ot eht debircserp level fo
1954
.seudiser
nI gnitroper eht stluser fo a tset ro sisylana edam ni ecnadrocca htiw this standard, fi eht lanif ,eulav
devresbo ro ,detaluclac is ot eb dednuor ,ffo ti shall eb enod ni ecnadrocca w$h SI 2 : 1960 .seluR‘
rof gnidnuor ffo laciremun seulav (
revised )‘.
SI 13829 : 1993
Indian Standard
PESTICIDE-METHODFORDETERMINATION
OFRESIDUESINAGRICULTURAEANDFOOD
COMMODITIES,SOILANDWATER-
ATRAZINEAND SIMAZINE
1 EPOCS 5.3 cihpargotamorhC ,nmuloC 25 mc gnol X 2 cm
.d.i
1.1 sihT standard sebircsed gas -otamorhc
cihparg ( CLG ) and hgih ecnamrofrep diuqil 5.4 yratoR muucaV rotaropavE
cihpargotamorhc ( CLPH ) sdohtem rof -imreted
noitan fo enizarta and enizamis seudiser ni doof 5.5 riA rewolB
.seitidommoc
5.6 retaW htaB
1.2 ehT timil fo noitanimreted fo htob eht
sdnuopmoc is 50’0 g/gp yb eht CLHP ,dohtem 5.7 saG hpargotamorhC
saerehw ti is 10’0 g/gp yb eht CLG .dohtem
deppiuqE htiw a negortin cificeps rotceted and
1.3 hguohT on tes erudecorp rof niht reyal gnitarepo rednu eht gniwollof detseggus psra-
yhpargotamorhc ( CLT ) is gnieb ,debircserp .sretem esehT sretemarap yam eb deirav
dezidradnats ( CLT ) serudecorp yam eb gnidrocca ot eht elbaliava ,qitilicaf dedivorp
,dewollof fi ,yrassecen rof eht esoprup fo naelc noitazidradnats is :enod
up, noitacifitnedi and noitamrifnoc fo seudiser
nmuloC : Glass, 100 mc ,htgnel 4 mm
fo enizarta and .enizamis
lanretni retemaid dekcap htiw
5 tnecrep 101-VO no morhcsaG
2 SECNEREFER Q ( 08-06 ) hsem
serutarepmeT : nmuloC nevo : 170°C
ehT naidnI Standards detsil woleb era yrassecen rotcejnI : 200°C
stcnujda ot this standard: rotceteD : 210°C
IS No. Title reirraC gas ( negortin ) wolf : nim/Im03
etar
1070 : 1992 tnegaeR edarg retaw ( driht
revision ) noitneteR emit : enizamiS : 3 setunim
yletamixorppa
11380: 1985 dohteM fo sampling rof eht enizartA : 5’3 setunim
noitanimreted fo edicitsep
yletamixorppa
seudiser ni larutlucirga and
doof seitidommoc
5.8 ertilorciM Syringe - 10 ~1 .yticapac

3. YTILAUQ FO STNEGAER 5.9 hgiH ecnamrofreP diuqiL hpargotamorhC
Unless deificeps ,esiwrehto erup slacimehc and deppiuqE htiw a elbairav evaw htgnel -artlu
dellitsid retaw ( ees SI 1070 : 1992 ) shall eb teloiv ,rotceted gnitarepo rednu eht gniwollof
deyolpme ni eht .stset detseggus .sretemarap esehT sretemarap yam
eb deirav gnidrocca ot eht elbaliava seitilicaf
NOTE - eruP‘ ’slacimehc llahs naem slacimehc
dedivorp noitazidradnats is:
taht od ton niatnoc seitirupmi hcihw tceffa eht
tluser fo .sisylana
nmuloC : xabroZ ODS ro lisitraP
ODS 25 mc~ htgnel and
4 GNILPMAS
6’4 mm lanretni retemaid
ehT evitatneserper samples rof eht esoprup fo eliboM esahp : lonahteM
gnitamitse seudiser fo enizarta and enizamis ni
wolF etar . * 1 nim/lm
eht seitidommoc shall eb drawn ni ecnadrocca
htiw SI 11380 : 1985. evaW htgnel : 230 mn
ecnabrosbA egnar : 20’0
5 SUTARAPPA
noitneteR emit : enizamiS : 6 setunim
yletamixorppa
5.1 Waring rednelB
enizartA : 8 setunim
2.5. yrotarobaL rekahS ( yratoR noitcA ) yletamixorppa
1
.
SI 13829 : 1993
6 STNEGAER dnelB eht erutxim rof 5 setunim and eunitnoc
rehtruf noitcartxe as debircsed ni 7.1.
6.1 mroforolhC - CLPIP edarg
7.3 yttaF ,sporC hcus as sdeesliO and Nuts
6.2 muelorteP rehtE - gniliob egnar 40-60°C.
wolloF eht noitcartxe erudecorp debircsed
6.3 lyhtE rehtE - 5 tnecrep ( v/v ) ni nobrac
ni 7.1. etaropavE mroforolhc yletelpmoc no eht
.edirolhcartet retaw bath htiw eht pleh fo maerts fo ria and
evlossid eht eudiser ni 50 lm muelortep .rehte
6.4 nobraC edirolhcarteT - CLPH .edarg refsnarT eht noitulos ot a lm-052 yrotarapes
lennuf and wash eht rekaeb eciwt htiw 20 lm
6.5 elirtinotecA - CLPH .edarg snoitrop fo muelortep rehte gnirrefsart eht
washings otni eht yrotarapes .lennuf tcartxE eht
6.6 lonahteM - CLPH .edarg muelortep rehte noitulos ni eht yrotarapes
lennuf ecirht htiw lm-52 snoitrop fo .elirtinoteca
6.7 muidoS etatecA reffuB noituloS looP eht elirtinoteca ,tcartxe and refsnart ot a
dnoces lm-052 yrotarapes lennuf and wash htiw
xiM lauqe semulov fo 2 N citeca dica and 1 N 50”ml muelortep .rehte dracsiD eht muelortep
edixordyhmuidos .noitulos rehte .reyal refsnarT eht elirtinoteca noitulos
,ylevitatitnauq yltcerid ot ehI dnuor mottob
6.8 detavitcaeR cisaB Alumina ksalf fo eht yrator muucav rotaropave and
yletelpmoc etaropave ffo eht elirtinotneca rednu
xiM 90 g cisab alumina htiw 10 lm retaw ylthgils decuder erusserp and htiw a retaw bath
ylhguoroht and wolla ot stand .thginrevo at 50°C.
6.9 citecA dicA - 2 N.
7.4 taeM and ggE +
edixordy noituloS - 1 N.
refsnarT a e’lbatius ytitnauq ( 002-001 g > fo
6.11 muidoS etabpluS - .suordyhnA eht elpmas otni a rrniraw rednelb gnola htiw an
lauqe ytitnaiq fo suordihna etahplcsYmuidos
6.12 lyhtE etatecA - AR .edarg and dnelb eht stnetnoc rof 5 .setunim Add
100 lm fo ,mroforolhc and ezinegomoh rof
rehtruf 5 .setunim eunitnoC eht noitcartxe as
6.13 ecnerefeR dradnatS enizartA - fo nwonk
rep eht erudecorp debircsed ni 7.1 and neht
.ytirup
eunitnoc as ni 7.3.
6.14 ecnerefeR dradnatS enizamiS - fo nwonk
.ytirup 7.5 Milk
7 NOITCARTXE refsnarT 200 lm fo eht klim elpmas otni a lm-006
,rekaeb and lm-OOZ lonahtem and xim
7.1 ,niarG ,wartS yaH ( woL erutsioM ) and ylhguoroht yb gnirrits htiw a glass .dor -ortnI

lioS ,ecud htiw ,gnirrits lm-02 muidos etateca reffub

.noitulos xiM llew and peek ni eht eci bath rof
refsnarT a elbatius ytitnauq ( 902-ool g ) ylecG 30 .setunim retliF eht erutxim hguorht a detulf
dnuorg elpmas otni a Waring ,rednelb add retlif repap and esnir eht retlif htiw a tej fo .retaw
lm-001 mroforolhc and ezinegomoh rof tcelloC eht etartlif and retaw washings ni a
5 .setunim refsnitrT eht stnetnoc ylevitatitnauq lm-006 .rekaeb refsnarT eht etartlif ot a 1 lm-OOO
ot a dereppots 1 lm-000 lacinoc ,ksalf add lm-004 yrotarapes lennuf and tcartxe ecirht htiw lm-06
mroforolhc dLa ekahs eht yrruls ylsuorogiv no a snoitrop fo .mroforolhc snoislumE nac eb
noitca-yrator yrotarobal rekahs rof eno .ruoh dediova yb eht noitidda fo a lm-2 detarutas-
retliF eht tcartxe yb ,gnitnaced hguorht a reyal noitulos fo muidos .edirolhc looP eht -orolhc
fo suordyhna muidos etahplus detnuom no a mrof stcartxe ni a lm-052 rekaeb and etaropave
Whatman .oN 1 ro tnelaviuqe retlif repap and a ot ssenyrd no a retaw bath htiw eht pleh fo a
.lennuf etoN eht tcaxe emulov fo eht etartlif maerts fo .ria
.deniatbo etaropavE an touqila fo eht ,tcartxe
tnelaviuqe ot 50 g fo eht porc ,elpmas ylluferac
7.6 retaW
ot ssenyrd no a retaw bath ni a lm-052 ,rekaeb
htiw eht pleh fo a eltneg maerts fo .ria
refsnarT 500 lm fo eht retaw elpmas otni a
7.2 tiurF and selbategeV 1 lm-OOO yrotarapes lennuf and tcartxe eht
suoeuqa reyal ecirht htiw lm-06 snoitrop fo
refsnarT a elbatius ytitnauq ( 100 g ) fo eht .mroforolhc looP eht mroforolhc stcartxe ni a
ylenif deppohc tiurf ro elbategev elpmas otni a lm-052 rekaeb and etaropave eht stnetnoc ot
Waring rednelb gnola htiw tuoba 100 g suordyhna ssenyrd no a retaw bath htiw eht pleh fo a
muidos etahplus and 100 lm fo .mroforolhc maerts fo .ria
2
IS 13829 : 1993
8 CLEAN UP As = kaep aera fo eht standard;
V, = ,emulov ni ,ly fo eht elpmas
Add 25 g detavitcaer cisab alumina ot eht
;detcejni and
cihpargotamorhc ,nmuloc tap yltneg ot etanimile
gnilennahc and ot eveihca mrofinu .gnikcap M = mass, ni g, fo eht elpmas nekat
evlossiD eht detcartxe elpmas eudiser ( ees 7 ) ni rof .sisylana
lm-ol nobrac ,edirolhcartet refsnart no ot eht
NOTE - tnecrep naem yrevocer si denimreted yb
nmuloc and wolla ot etartenep otni eht alumina.
gnikat detaertnu lortnoc elpmas ot hcihw a nwonk
Wash eht rekaeb htiw 10 lm fo nobrac -artet tnuoma fo enizarta ro enizamis si dedda dna
edirolhc and refsnart ot eht nmuloc dezylana sa debircsed .evoba
gniwolla ot etartenep as .erofeb sihT noitarepo
shall eb rehtruf detaeper htiw rehtona 5 lm fo 10 HIGH PERFORMANCE LIQUID
.edirolhcartet nehW eht tnevlos has just CHROMATOGRAPHIC ( HPLC > METHOD
detartenep otni eht ,nmuloc add OS lm fo nobrac
edirolhcaltet and wolla ot pass hguorht eht 10.1 Principle
alumina .reyal nehW eht last pord fo eht nobrac
edirolhcartet has deniard ,nwod ecalp a naelc ehT seudiser fo enizarta ro enizamis detcartxe
lm-052 rekaeb as ,reviecer and add 100 lm fo 5 morf eht elpmas retfa naelc up is devlossid ni
tnecrep lyhte rehte ni nobrac edirolhcartet lonahtem and detamitse yb CLPH deppiuqe htiw
gnitcelloc eht etelpmoc ctaule ni eht .rekaeb teloiv-artlu .rotceted ehT tnetnoc fo enizarta
etaropavE eht stnetnoc fo eht rekaeb ot ssenyrd ro enizamis is denimreted yb gnirapmoc eht
no a retaw bath htiw a maerts fo .ria esnopser htiw eht esnopser fo a nwonk standard
fo ralimis .noitartnecnoc
9 GAS CHROMATOGRAPHIC METHOD
10.2 Procedure
9.1 Principle
evlossiD eht eudiser retfa naelc u$ ( ees 8 > ni
ehT eudiser fo enizarta ro enizamis detcartxe lm-2 lonahtem and .tcejni 5 ~1 fo this noitulos
morf eht elpmas retfa naelc up is devlossid ni otni eht CLPI-I .tnemurtsni yfitnedI eht kaep
lyhte etateca and detamitse gas -ihpargotamorhc rof enizarta ro enizamis and erusaem eht kaep
yllat ni an tnemurtsni deppiuqe htiw a negortin .aera
cificeps .rotceted ehT tnetnoc fo enizarta ro
enizamis ni eht elpmas is denimreted yb 10.3 noitaluclaC
gnirapmoc eht esnopser htiw eht esnopser fo a
nwonk standard fo ralimis .noitartnecnoc eudiseR fo enizarta ro enizamis ( g/gp >
9.2 Prscedare IA x
ZA
evlossiD eht eudiser retfa naelc up ( ees 8 ) ni
erehw
lm-2 lyhte etateca and tcejni 2 folm this noitulos
otni eht gas .hpargotamorhc yfitnedI eht kaep A1 = kaep aera fo eht ;elpmas
rof enizarta ro enizamis yb eht noitneter emit
and erusaem eh$ kaep .aera v, = ,emulov ni ,lu, fo standard
enizarta ro enizamis ;detcejni

9.3 Calculation v, = latot ,emulov ni ,lm fo eht
elpmas ;noitulos
eudiseR fo enizarta
se Al x V, x V, x c C= ,noitartnecnoc ni ,g/gp fo eht
ro enizamis
fx standard ;snoitulos
A2 x V, x M
erehw f= yrevocer rotcaf
A, = kaep aera fo eht ;elpmas 100
=
tnecrep naem ;yrevocer
V, = ,emulov ni ~1, fo standard
enizarta ro enizamis ;detcejni AZ = kaep aera fo eht standard;
,v = latot ,emulov ni ,lm fo eht v, = ,emulov ni ~1, fo eht elpmas
elpmas ;snoitulos ;detcejni and
c = ,noitartnecnoc ni g/gLt fo eht M= mass, ni g, fo eht elpmas nekat
standard ;noitulos rof .sisylana
f = yrevocer rotcaf ETON - tnecreP naem yrevocer si denimreted yb
100 gnikat detaertnu lortnoc elpmas ot hcihw a nwonk
tnuoma fo enizarta ro enizamis si dedda dna
= tnecrep naem yrevocer ’ dezylana sa debircsed .evoba
dradnatS- Mark
ehT esu fo eht dradnatS Mark is denrevog yb eht snoisivorp fo eht
Bureau of Indian
and eht seluR and snoitalugeR edam .rednuereht ehT dradnatS Mark no
Standards Act, 1986
stcudorp derevoc yb an naidnI dradnatS syevnoc eht ecnarussa that yeht evah neeb decudorp
ot ylpmoc htiw eht stnemeriuqer fo that standard rednu a llew denifed metsys fo ,noitcepsni
gnitset and ytilauq lortnoc hcihw is desived and desivrepus yb SIB and detarepo yb eht -orp
.recud dradnatS dekram stcudorp era osla ylsuounitnoc dekcehc yb SIB rof ytimrofnoc ot
that standard as a rehtruf .draugefas sliateD fo snoitidnoc rednu hcihw a ecneci rof eht esu
fo eht dradnatS Mark yam eb detnarg ot srerutcafunam ro srecudorp yam eb deniatbo morf
eht uaeruB fo naidnI Standards.
Bureau of Indian Standards .
SIB is a yrotutats enoitutitsni stablished rednu eht ot otomorp
Bureau of Indian Standards Act, 1986
suoinomrah tnempoleved fo eht seitivitca fo ,noitazidradnats gnikram and ytilauq noitacifitrec fo
sdoog and gnidnetta ot detcennoc srettam ni eht .yrtnuoc
Copyright
SIB has eht thgirypoc fo all its .snoitacilbup oN trap fo eseht snoitacilbup yam eb decudorper ni
yns mrof tuohtiw eht roirp noissimrep- ni gnitirw fo .SIB sihT seod ton edulcerp eht eerf ,esu ni
eht esruoc fo gnitnemelpmi eht standard, fo yrassecen ,slialted hcus as slobmys and ,sezis epyt ro
edarg .snoitangised seiriuqnE gnitaler ot thgirypoc eb desserdda ot eht rotceriD ( snoitacilbuP ,) .SIB
Review of Indian. Standards
stnemdnemA era issued ot standards as eht deen sesira no eht basis fo .stnemmoc Standards era
oslra deweiver ;yllacidoirep a standard gnola htiw stnemdnema is demriffaer nehw hcus weiver
setacidni that on segnahc era ;dedeen fi eht weiver setacidni that segnahc era ,dedeen ti is nekat
up rof .noisiver sresU fo lndian Standards dluohs niatrecsa that yeht era ni noissessop fo eht
tsetal stnemdnema ro noitide yb gnirrefer ot eht tsetal issue fo SIB‘ ’koobdnaH and sdradnatS‘
ylhtnoM .’snoitiddA stnemmoC no this naidnI dradnatS yaM eb tnes ot SIB gnivig eht gniwollof
ecnerefer :
toD : No. FAD 34 ( 0010 )
Amendments Issued Since Poblication ,
dnemA .oN etaD fo eussI txeT detceffA
UAERUB FO NAIDNI SDRADNATS
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