IRRADIANCMEICRANODPROAPBASGCAITSEICDAACRIODNIAEFFAERBCUTTSIOFNOLIAACCL(ILM.A)TEILZLATIONOF WILFREDOCOLON-GUASP OFATHDEISSUOENFRITVTAEHETRSIIORTNEDYQOUPCOIRTFREOESRMEFENLONTOFTERSDIPDHTAFIOOLRIONTSHTOEHPPEAHGRYRTDAIEDGARULEAETFEUOLFFSICHLOLOMLENT UNIVERSITY FLORIDA ThisworkisdedicatedtomydaughterVeronica motherJosefina. ACKNOWLEDGEMENTS DcsDar.oce.mawmdaDieyaTtMmnvetyieirecermesLio,c.lsaatrDneIrdsAsen.i.rgKn.recaMnNeminercelehtlfaohaerplapJn.rhdEei.cBsiotKaoaocttnoieeno.,ttnhieJniTuashammaeleenxsmkpsbrsEuee.prsapssrBoeeardrtoerftxeotttthemm,rnyyoduecFgdhdrhaoeoitcdurotetmorariDnarcm,.lky Thanksareextendedtoallthemembersofthe FloriculturegroupandtheTissueCultureLaboratoryfortheir technicalsupportandpatience. theNaItwiiosnahltoHisapcaknniocwleSdtguedenttheFUenlilvoewrsshiitpyfoorfPthueeirrtosuRpipcoorta.nd friendIshaimp,thsaunpkpfourlt,toanadllentchoeurgargaedmueanttetshtruoduegnhtosutfomrytghreaidruate workattheUniversityofFlorida. providFiinnagllmye,wIiwtahnthitsotghraanckeoaunrdLofrodrJbelseusssiCnhgrismtefwoirthan extendedchurchfamily. “’ilsls»!ll#3“f^ "lipg»l“ underinvitroconditions(Experiment4) 'sskh| HJSls ISduring30daysexvitroat450 Figurerloeoa3ft-e7.ds^omlIiuncbfrlloeuceunstcutegianrogfscdfouonrutirenngtle3vo0efldsaAyrosofneixAaBvAairtb(rumogt/iaLft)ol6lo5an0 Figuremanrir1ec3ars-')8o1.cu(oEtEfftxfipeenAcrgrtisosmneginoratfowaA4nr)BbAiuntoinvfiwotalrtioearfollreoas3vs0esd(amygesxHc,ai0ts/ec4md5'/lfiermaoofml ofinvitrogrownmicrocuttingswasnegative.Exvitro,Awas lowerforABAtreatedmicrocuttingsthanforcontrolplants. TheeffectsofexogenousABAtreatmentspersistedupto10 daysposttransplantexvitro.At20daysposttransfer, growthandAweresimilarinalltreatmentsandcontrols. Nonstructuralcarbohydratecontentinleafandstem tissueofmicrocuttingswasalteredwithABAtreatments. Underinvitroconditionsandafter5daysofexvitrogrowth, leafsolublesugarcontentwashigherinABAtreatedplants thancontrols.Leafchlorophyll,carotenoid,andleafwater losswereunaffectedbytheABAtreatmentsappliedinvitro. Underexvitroconditions,rootedmicrocuttingswere placedundertwoirradiancetreatmentsof450and650pmol mo'v!ers'a.llgTrhoewthhi,ghwehrileirrnaodnisatnrcuectulreavlelcsarcbaouhsyeddraaterecdounctteinotnwaisn grroeoatteedrmitchranocauttt4i5n0gspmgorlownn’as't1.45L0igwhetrecolmopweenrstahteinonaptoi65n0tspmfoolr Insummary,inA.arbutifoliamicrocuttings,endogenous ABAplayedanimportantroleinleafwaxdevelopmentand increasedtotalnon-structuralcarbohydratesoftheleaves comparedtocontrolplants. Whenappliedinvitro,this growthregulatorhasthepotentialofpre-hardeningtheleaf priortotheonsetofwaterstressencounteredat transplantingintoanexvitroenvironment.