Interorgan exchange of Amino Acids

67 Pages·2007·1.94 MB·English

by  Mick Deutz

Interorgan exchange of Amino
Acids: What is the driving
force?
NEP Deutz
Center for Translational Research in Aging
& Longevity. Donald W. Reynolds Institute
on Aging. UAMS, Little Rock
Interorgan exchange of Amino Acids
Introduction
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Methods to measure interorgan exchange of
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Amino Acids
Interorgan exchange Amino Acids during feeding
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Impact Diet on interorgan exchange
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Impact disease on interorgan exchange
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Conclusion
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Interorgan exchange of Amino
Acids
Is highly active and regulated process
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Provides and delivers Amino Acids to all organs
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and tissues for
protein synthesis
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Specific metabolic functions
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Plays a role in the maintenance of plasma Amino
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Acid homeostasis
Major role of (patho)physiological state and
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nutrition
Interorgan exchange of Amino
Acids
After Feeding and initial phase of fasting
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Dominant flux from the Gut to other tissues
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During prolonged fasting
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Dominant flux from muscle to liver and kidney
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Interorgan Amino Acid transport: Transition from feeding and early
fasting period to prolonged fasting and starvation
Amino Acids
A
mi
n
o Acids A m in o A c i d s Amino Acids
Amino Acids
FASTED
FED
Interorgan exchange of Amino Acids
Introduction
(cid:132)
Methods to measure interorgan exchange of
(cid:132)
Amino Acids
Interorgan exchange Amino Acids during feeding
(cid:132)
Impact Diet on interorgan exchange
(cid:132)
Impact disease on interorgan exchange
(cid:132)
Conclusion
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Peroperative Interorgan
measurement
Direct
puncture of
portal vein
(either blind
or direct)
Catheter
placement in the
pig (full model)
To measure plasma
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flow, PAH is infused
through the splenic
vein (S) and
abdominal aorta (A1)
For turnover
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measurements, tracer
is infused through the
caval vein (V2) for
turnover
measurements
Blood is sampled from
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the abdominal aorta
(A2), caval vein (V1),
hepatic vein (H) and
portal vein (P)
Lab Anim 30(4): 347-58
The Interorgan Pig Model
Implantation of the
splenic and portal
vein catheter
Lab Anim 30(4): 347-58
The Interorgan Pig Model
Implantation and
checking of the
position of the
hepatic vein
catheter
Lab Anim 30(4): 347-58