Interaction between the Basolateral K+ and Apical Na' Conductances in Necturus Urinary Bladder

18 Pages·2003·0.99 MB·English

(cid:9)(cid:9)(cid:9)(cid:9)
Interaction between the Basolateral K and Apical
+
Na' Conductances in Necturus Urinary Bladder
JEFFERY R. DEMAREST and ARTHUR L. FINN
Fromthe DepartmentsofMedicineand Physiology, University ofNorthCarolina School
ofMedicine, Chapel Hill, North Carolina 27514 Do
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ABSTRACT Experimentalmodulation of the apical membrane Na' conduct- fro
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ance or basolateral membrane Na'-K' pumpactivity has been shown to result h
in parallel changes in the basolateral K+ conductance in a numberofepithelia. ttp://ru
To determine whether modulation of the basolateral K' conductance would p
re
result in parallel changes in apical Na* conductance and basolateral pump ss.o
activity, Necturus urinary bladders stripped of serosal muscle and connective rg/jg
tissue wereimpaled throughtheir basolateral membranes with microelectrodes p/a
in experiments that allowed rapid serosal solution changes. Exposure of the rticle
basolateral membrane to the K+ channel blockers Bat+ (0.5 mM/liter), Cs' (10 -pd
f/8
mM/liter), or Rb' (10 mM/liter) increased the basolateral resistance (Rb) by 9/4
>75% in each case. The increases in Rb wereaccompanied simultaneously by /56
3
significant increases in apical resistance (R.) of>20% anddecreases in transep- /12
4
6
ithelialNa' transport.TheincreasesinR.,measuredasslope resistances, cannot 6
8
3
be attributed to nonlinearity of the I-V relationship of the apical membrane, /5
6
3
since the measured cell membrane potentials with the K' channel blockers .p
d
present were not significantly different from those resulting from increasing f by g
serosal K+, a maneuver that did not affect R.. Thus, blocking the K+ conduct- ue
ancecauses a reduction in net Na' transport byreducingK+ exit from the cell st o
n
andsimultaneously reducing Na'entry intothe cell. Closecorrelations between 30
the calculatedshort-circuitcurrent andthe apical and basolateral conductances Jan
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a
were preserved after the basolateral K+ conductance pathways had been ry 2
blocked. Thus, the interaction between the basolateral and apical conductances 02
3
revealedbyblocking thebasolateral K*channels ispartofa network offeedback
relationships that normally serves to maintain cellular homeostasis during
changes in the rate oftransepithelial Na' transport.
INTRODUCTION
For some timeit has been known that thereare important feedback mechanisms
that couple the passive membrane permeabilities to the activity ofthe Na' pump
in Na'-transporting epithelia (Schultz, 1981; Diamond, 1982). MacRobbie and
Address reprint requeststo Dr.Arthur L. Finn, Depts.ofMedicineand Physiology, University
of North Carolina at Chapel Hill, Old Clinic Bldg. 226H, Chapel Hill, NC 27514. Dr.
Demarest's present address is Physiology-Anatomy Dept., University of California, Berkeley,
CA94720.
J.GEN. PHYSIOL. ©The RockefellerUniversity Press- 0022-1295/87/04/0563/18$1.00 563
Volume 89 April 1987 563-580
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564 THEJOURNALOF GENERALPHYSIOLOGY " VOLUME 89 " 1987
Ussing (1961) were the first to show that inhibition ofthe Na' pump caused a
decrease in both the apical and basolateral ion permeabilities in the frog
skin. This finding hassubsequently been confirmed by other investigators using
isotopic tracers and intracellular microelectrode techniques (Chase and
Al-Awgati, 1979; Helman et al., 1979). Reuss and Finn (1975) were thefirstto
report electrical interactions betweentheapical andbasolateralmembranes that
could not be attributed to changes in current flow through the parallel shunt
pathway.Thealteration oftheapical membrane electromotiveforce(emf)caused
by theapplicationof mucosalamiloride or by changing the ioniccomposition of
the mucosal bath resulted in a rapid change in the basolateral membrane emf
D
(Reuss andFinn, 1975; Finn and Reuss, 1978; Narvarte and Finn, 1980). More ow
n
recently (Davis and Finn, 1982a, b), it has been shown that inhibition of the loa
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apical membrane Nay channel results in the inhibition of the basolateral K+ d
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conductance.Theseinteractions have been interpretedas homeostaticregulatory m
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mechanisms that servetomaintain steady stateintracellularionic concentrations ttp
andcell volume during changesin the rates oftranscellular ionand waterflux. ://rup
Intheprecedingarticle(Demarestand Finn, 1987),we demonstrated that the ress.o
dominant factor determining the membrane potential in the basolateral mem- rg
/jg
braneoftheNecturus urinarybladderis ahighlyselectiveK+conductance. Other p/a
studies have shown that the activity of basolateral K' channels of epithelia is rticle
dependenton the volume ofthecellsand canbe regulatedby hormones (Nagel -pd
f/8
andCrabbe, 1980; Davisand Finn, 1982a; Maruyama and Petersen, 1982; Lau 9/4
et al., 1984). Both effectsappear to be mediated throughchangesin intracellular /56
3
Ca" (Maruyama and Petersen, 1984; Davis and Finn, 1985). The central role /12
4
6
thatsuch K+conductancepathwayshavein thewidely accepted KoefoedJohnson 68
3
and Ussing (1958) model oftransepithelial Na' transport raises the question of /56
3
whetherthe K' channel is an importantsite forthe regulationof Na'transport. .pd
f b
Is there a tight coupling between the basolateral K+ and apical Na' channels? y g
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Bat+, a known blocker of K+ channels, has been shown to inhibit net Na' est o
transportby epithelia, butits site ofactionhasbeen amatter ofdispute(Ramsay n 3
et al., 1976;Nagel, 1979; Hardcastle et al., 1983). Inthis study, Bat' andseveral 0 Ja
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other K' channel blockers have been used to investigate interactions between ua
the passive membrane permeabilities ofNecturus urinarybladder. Blocking the ry 20
2
basolateral K' conductance results in an immediate reduction of apical Na' 3
conductance that is not mediated through changes in membrane potential.
Preliminary reports of these studies have been presented elsewhere (Demarest
and Finn, 1983, 1984).
METHODS
Urinarybladders from male Necturus maculosus (NascoBiological Supply, Ft. Atkinson,
WI) were mountedhorizontally, serosal side up,in an open-topped Lucite chamberthat
was placed on the stage ofthe inverted microscope (Diavert, E. Leitz, Inc., Rockleigh,
NJ) used to view the epithelial cells at 320X with bright-field illumination during the
experiments. Detailsofthe experimental methodsare describedin theprecedingarticle
(DemarestandFinn, 1987). Briefly, mostoftheserosal muscleand connective tissue was
removed from the basolateral surface of the epithelial cell layer and microelectrode
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DEMAREST AND FINN Interaction between K'and Na'Channels 565
impalements weremadeacross the basolateral membranes ofthe cells. Both sidesofthe
epitheliumwere continuouslyperfused usinga system that allowed rapidchanges in the
composition ofthe serosal bathing solution.
Solutions
The Necturus Ringer solution had the following composition (mM/liter): 95 NaCl, 10
NaHCO3, 2.0 CaC12, 1.0 MgCl2, 1.19 K2HP04, 0.11 KH2PO4, 5 glucose. The total
osmotic concentration was 210 mosmol/kgand the pH was 7.9 when equilibrated with
99% 02, 1% C02. In solutions with ahigher-than-normal [K+](i.e., >2.5mM), KCl was
substituted for NaCl to obtain the concentrations indicated in the text. RbCl and CsCl
weresubstituted forNaClasindicatedin thetext. Amiloride (agiftfromMerck, Sharp&
D
Dohme Research Laboratories, West Point, PA) was dissolved in Necturus Ringer at a ow
n
finalconcentration of 10'4 M.Verapamil (Calbiochem-Behring Corp., LaJolla, CA) was lo
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dissolved in Necturus Ringer at a final concentration of 10"4 M. The experiments were ed
performed atroomtemperature (23 ± 1°C). fro
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ElectricalMeasurements ttp://ru
measurements and circuit analysis were made as described in the preceding pre
article (Demarest andFinn, 1987). ss.o
The emfofthe shunt pathway was assumed tobezerowhen the solutions on thetwo rg/jg
p
asrideesdiosfcutshseedepiintthheeliRuemsuwletrse. identical; experiments employing nonsymmetrical solutions /article
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Statistics f/89
/4
Allmeanvaluesaregivenwithstandarderrors(mean±SE). Comparisons between means /56
3
were made usingthe t test forpaired data. Coefficients andintercepts for least-squares /12
4
regression linesare given withstandard deviationsandwere compared usinganalysis of 66
8
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variance. /5
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3
.p
RESULTS df b
y g
Effects ofBa'fon the Measured ElectricalProperties ue
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The addition of 0.5 mM Ba2+ to the serosal solution (Fig. 1) caused a rapid n 3
0
depolarization ofV,,, which reachedanew steady state in -7 s. Simultaneously, Ja
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Vwe,r,ewaasccshoimfptaedniinedthbeyneagadteicvreeadsiereicntitohne.Trahteiocohfantgheesdienfltehcetimoensmborfatnhee ppootteennttiiaallss ary 20
2
3
gfrom transepithelialcurrent pulses(Ra/Rb), which indicated anincrease
in the relative resistance ofthe basolateral membrane. 11 experiments, in which
measurements weremadein the quasi-steady state30safterthe addition of0.5
mM/liter serosal Ba", are summarizedinTable 1. Fromthe significant decrease
the increase in R,, itcan be calculated thatthe short-circuit current
fell from 29 ± 6 to 20 ± 4 gA-cm-2 (P < 0.01, n = 11), which indicates a
significant inhibitionofnet Na' transport. Higher concentrations ofserosal Bat+
did not produce significantly greater effects (e.g.,in eightexperiments, 1.0mM/
liter Bat+depolarized V,, to 44.7 ± 3.3 mVand reduced Ra/Rbto 3.66 ± 0.55;
theseeffects were not significantly different fromthose shown in Table 1).
Increasingserosal K from 2.5 to 50 mM/liter depolarized V,5 by 32.7 ± 2.5
mV, increased Ra/Rb from 3.27 ± 0.60 to 6.36 ± 0.55, and decreased R, by
>20% (Demarest and Finn, 1987). These effects ofincreased serosal K+ were
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566 THEJOURNAL OF GENERAL PHYSIOLOGY " VOLUME 89 - 1987
50 mV
3s
0-
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FIGURE 1. The effect ofBat+on thecell membrane potentials. The recordstarts fro
withamicroelectrode in the cell. The uppertraceis theapical (Vm,)and thelower m h
twrhaiccehiswtehreebmaseoalsautrereadlw(iVct.h)mreefmebrrenacneetpoottehnetisaelro.sUalpwbaarthdinisgpsoosliuttiivoenfoarsbgortohutnrdac.eAst, ttp://rup
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thearrow, theserosal solutionwas changed toRingerwith0.5mM/literBaC12. The ss.o
repeated downward deflections in the traces were due to transepithelial current rg
pulses (5 pA.cm-2 for 500 ms). The ratio ofthe deflections (AV.,/AV,, = Ra/Rb) /jgp/a
decreased, which indicates an increase in the relative resistance ofthe basolateral rticle
membrane. -p
d
f/8
9
/4
significantly attenuated in the presence of serosal Bat+ (V,s was depolarized by /563
13.3 ± 2.9 mV, Ra/Rb increased from 1.72 ± 0.19 to 3.42 ± 0.42, and R, /124
6
decreased by 8%) as compared with its absence, whichis consistent with partial 68
blockade ofthe basolateral K' conductance by Bat+. 3/56
3
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EffectsofBat-1on theMembrane ResistancesandElectromotiveForces f b
y g
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TinhethvealsuteesaodyfRsat,atReb,aEftae,ranBdaEt+b,,caarlecuslhaotewdnfrinomTatbheledaItIa.oIfnTaablseepIarbaetfeorseetanodf est on 3
experiments on five bladders, Bat+ was found to have no significant effect on 0 Ja
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the shunt resistance, Rs, which was 6.24 ± 1.25 kQ-cm2before and 6.26 ± 1.37 ua
after serosal Bat+. Bat+ caused an increase in Rb and a depolarization ofEb. ry 2
0
2
3
TABLE I
EffectsofSerosalBas+(0.5mM)on the
TransepithelialandCellular Electrical Properties
V., V- V<. R.IRb R,
MV k12-cm'
Control -86.6±5.8 -16.5±5.1 -68.8±3.9 5.25±1.36 4.16±0.69
Ba2` -73.3±5.9 -32.7±5.3 -40.1±3.9 3.16±1.15 4.81±0.78
0 13.4±1.8 -16.7±2.0 28.7±2.9 2.09±0.38 0.64±0.15
P <0.001 <0.001 <0.001 <0.001 <0.01
Measurements were madebycontinuouslyrecordingin single cells. Valuesaremeans
±SE for11 bladders.Ais thedifference betweencontrolandBa4*-treatedtissues.
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DEMAREST AND FINN Interaction between K+and Na'Channels 567
TABLE II
Effect ofSerosalBaY+(0.5mM)onthe Cell
MembraneResistancesand ElectromotiveForces
R, Rb E, Eb
ktl.em2 W-cm, mV mV
Control 7.82±1.88 1.74±0.37 -93±17 -86±5
Bat+ 9.82±2.53 3.95±0.98 -112±19 -73±8
A 2.00±0.85 2.21±0.73 18±2 14±4
P <0.05 <0.02 <0.001 <0.02
Values werecalculated fromthe data inTableIasdescribed inthe text.R, was 8.24
D
kQ CM2.Aisthe differencebetween control andBa'-treatedtissues. ow
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Table III showsthe effects of increased serosal K' on the membrane resistances fro
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andemf's ofBat+-treated bladders. In theabsence ofserosal Bat+, 50 mM/liter h
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serosal K+ did not significantly affect Ra (see Fig. 2) or Ea, but reduced Rb by ://ru
>50% anddepolarizedEb by 53± 5 mV (Demarestand Finn, 1987). However, pre
in the presence of serosal Bat+ (Table III), the effect on Eb was significantly ss.o
rg
attenuated. Thesedata indicate that Bat+ significantly reduces the transference /jg
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nthuemebfeferctofofthBeabta+soolnatRebraclanmebembarttarniebutfeodrtKo+a(pDaermtiaarlesbltoacnkdofFitnhne,ba1s9o8l7a)te.rTahluKs,+ /article-p
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conductance. However, serosal Bat+also caused a significant increase in Ra and f/8
9
a hyperpolarization ofEa (Table II). Fig. 2 shows that these increases are not /4/5
6
simply due to a nonlinear current-voltage relationship of the apical membrane, 3/1
2
since the values of Vmc before and after either 50 mM/liter serosal K+ or 0.5 46
6
mM/liter serosal Bat+ were not significantly different, yet increased K+ did not 83/5
change Ra significantly. Thus, blocking the basolateral K+ conductance resulted 63.p
d
in a decrease in apical Na+permeability that wasreflectedas an inhibition ofthe f b
I.,. The Bat+-induced hyperpolarization of Ea is consistent with a drop in y gue
st o
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3
0
Vmc ARa Jan
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control experimental ry 2
0
2
3
FIGURE 2. Comparison oftheeffectsof50 mM K+and 0.5 mM Ba2+ on Vm, and
the change in R, (AR.). Although the values of V., after K+ or Bat+ were not
significantly different, only Bat+ resulted in asignificant(P<0.05) increase in R,.
(cid:9)
THEJOURNALOF GENERAL PHYSIOLOGY " VOLUME 89 - 1987
A
7.5
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R 5.0
(fl.cm
2.5
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0 10 20 30 a
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Time (seconds) fro
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p
7.5 re
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/jg
R p/a
(!2"cm2) 5.0 rticle
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f/8
9
/4
2.5 /5
6
3
/1
2
4
6
6
8
3
/5
0 10 20 30 63
.p
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Time (seconds) f by g
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FIGURE 3. Time course ofthe changes in the cell membrane resistances caused st o
by Bat+. Two types of time course for R, were observed. (A) In bladders with n 3
0
transepithelial potentials, V,,, greater than ^-70 mV, R, exhibited a biphasic re- Ja
sponse. (B) In bladders with V,sless than ^-70 mV, R, increased monotonically to a nua
quasi-steady state. R,,increased monotonically in all bladders. ry 2
0
2
3
TABLE III
EffectofIncreasingSerosalK'to 50 mMon theCell
MembraneResistancesandElectromotiveForces ofBa2'-treatedBladders
Rb E, Eb
kfl-cm' kfl-cm' mV mV
Bat' 5.97±1.63 3.26±0.58 -81±12 -80±4
Bat'+50 tnM [K'], 5.27±1.16 1.52±0.31 -71±14 -41±3
0.90±0.62 1.73±0.37 9±11 39±2
P NS <0.005 NS <0.001
n = 6.NS,no significantdifference. A is thedifference betweentissuestreated with
Bat'aloneandthoseto whichboth Bat'andK' were added.
(cid:9)(cid:9)(cid:9)
DEMAREST AND FINN InteractionbetweenK' andNo'Channels 569
intracellular Na' resulting from the continued activity of the Na'-K+ ATPase
whilethe rateofapical Na' entry was decreasing.'
Time Course oftheBat'Effects on theCellMembrane Resistances
Two different time courses of Bat+-induced changes in Ra were encountered
that weredependent upon the valueofV.,after Bat+. Ifthe Bat'-induced value
ofV,cwas morenegative thanabout-10 mV, Ra exhibited a biphasic response
(Fig. 3A). Itfirst decreased to a minimum at 9.6 ± 0.8s, which was significantly
(P < 0.01) lower than control. By 20.4 ± 2.4 s, Ra had returned to a level not
significantly different from control, and then continued to increase toa quasi-
steadystate significantly (P<0.05)higher thancontrol by 30 s. Inbladderswith Dow
aBa2'-induced V.,morepositivethan -10mV, Ra increased monotonically toa nlo
a
quasi-steady state significantly (P<0.05) higher than control by 20 s (Fig. 3B). ded
The Ba2'-induced increase ofRb, hyperpolarization ofEa, and depolarization of from
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FBIaGt+U.RTEhe4r.eCwoarsrealastiignoinfibceatntweceonrrtehleatcihoann(gre=in0R.a8,10A,RP., <an0d.V0.0,1)atbe1t0wseaefnteDrRsearoasnadl f by gue
V,,.The solidline (OR, = 0.016 ± 0.003,Vm,- 1.466 ±0.310;P<0.001,n= 16) st o
n
was fitted tothedataby least-squares regression. 3
0
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Eb(Table II) were monotonic in all casesand reachedsteady statelevelsby 20 s. ua
Notethat the Bat'-induced changesin V.,and Vc,had reached theirsteadystate ry 2
0
2
levels within 5-7 s (Fig. 1). 3
There wasasignificant correlation (r = 0.810,P< 0.01) between the change
in Ra (AR,,), and V,c at 10 s after Bat+ (Fig. 4), but no significant correlation (r
= 0.063, P > 0.5) between the quasi-steady state values. In addition, there was
nosignificant difference between the mean steady state values ofRa for bladders
that exhibited biphasic (Ra = 8.15 ± 1.80 kQ-cm2) and monophasic(Ra= 8.79 ±
2.10 kQ.cm2)timecourses.
' Inthehigh-V_winterbladdersofTableII,Eaappearstobeexclusively anNa'emf(Demarest
and Finn, 1987).Calculating theintracellular Na'concentrationfrom the values ofEa (Table
II) using the Nernst equation givesa value of 2.6 mM/liter undercontrol conditionsand 1.2
mM/liter after serosal Bat+. The former value for control conditions is about half of that
estimatedinpreviousstudies onNecturus urinarybladderbyfitting the constant fieldequation
to the current-voltage relationship oftheamiloride-sensitive apical Na'pathway (Fromteret
al., 1977;Thomas etal., 1983).
(cid:9)(cid:9)(cid:9)(cid:9)(cid:9)(cid:9)(cid:9)(cid:9)
570 THEJOURNALOF GENERAL PHYSIOLOGY " VOLUME 89 " 1987
TABLE IV
EffectofVerapamil(100pM)on theResponse to SerosalBap+
Withoutverapamil With verapamil
V, R,/Rb R, V RJRb R,
mV k12.cin' MV kg-cm'
Control -68.7±1.8 3.72±0.75 1.76±0.42 -65.3±2.8 3.15±0.52 1.79±0.43
Bas* -37.4±2.9 1.90±0.31 1.98±0.44 -31.6±2.3 1.60±0.36 2.14±0.41
31.3±1.8 1.82±0.77 0.22±0.06 33.8±1.0 1.55±0.32 0.35±0.13
n=4.Ais thedifference betweencontrolandBas'-treatedtissues.
D
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Effects ofVerapamil on theBat+Response w
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a
VerapamilblocksCa channels that exhibithigh conductances forBat+ (Hagiwara de
d
andByerly, 1981).Averapamil-sensitiveCachannelhasbeenreported in isolated fro
m
toad urinarybladdercells(Humes etal., 1980). To determinewhether theeffect h
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of Bat+ on Ra was due to Bat' entry into the cells, the effects of Bat+ were ://ru
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examined in bladders treated with verapamil (100 1M). Table IV shows that re
there was no significant effect of serosal verapamil on the electrical properties ss.o
rg
or theresponses of the bladders to Bat+. Furthermore, the effects of Bat+ were /jg
p
fthoiusnsdtutdoyb(e<Ico0mmpilne)te.lTyheresveerdsaitbalesufgogretshtethsahtorBtate'xpdooseusrenottimeexseritnvitessteifgfeacttesd biny /article-p
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entering the cells. f/8
9
/4
Effects of Cs+ and Rb+ on the Cell Membrane Resistances and Electromotive /56
3
/1
Forces 2
4
6
6
Fig. 5 shows the effects on V,, of raising serosal K+ from 2.5 to 50 mM/liter 83
/5
6
3
50 [K]S .pd
10 [Cs],1 10 [Rb]j f by g
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st o
n
3
1c8 0 Ja
n
u
a
ry 2
0
2
3
Vcs 70.1 30.8 68.4 54.3 36.9 69.9 45.8 28.6
(mV) ±1.6 ±1.5 t1.9 ±4.7 ±5.6 ±2.9 ±4.1 ±2.8
AVCs 39.3 14.1 17.4 24.1 17.1
±1.6 ±4.5 t4.1 t2.3 t2.4
FIGURE 5. TheresponseofV., to 50 mM/literserosalK+undercontrolconditions
(left),in thepresence of10mM/literserosalCs+(center),andin thepresence of 10
mM/liter serosalRb+(right). RecordsofV, forthreecellsin differentbladders are
shown. Each record starts with a microelectrode in thecell. The horizontal filled
barsat thetopofthefigure indicate thetime periodswhen serosalK+waselevated
from 2.5 to 50 mM/liter. Theopen bars indicate the presence of 10 mM/liter Cs'
(center) or 10mM/liter Rb+ (right). The mean values of V, andthe change in V_
AV_areshownacross thebottom ofthefigure.
(cid:9)(cid:9)
DEMARFST AND FINN Interaction between K+andNa'Channels 571
TABLE V
EffectsofSerosal Cs(10mM) onCell
MembraneResistanceand ElectromotiveForces
R, Rb E, Eb
k12-cmz k12.cm2 mV mV
Control 7.30±1.18 1.78±0.28 -101±10 -96±4
Cs 9.14±1.38 3.15±0.32 -108±10 -93±5
1.84±0.50 1.37±0.32 -7±8 3±4
P <0.01 <0.01 NS NS
n = 11. NS, no significant difference. Ais the differencebetweencontrol and Cs-
D
treated tissues. ow
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a
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d
under control conditions and in the presence of 10 mM/liter Cs' or Rb+. Both from
ions significantly reduced the effect of increasing serosal K+. The high-K+- http
induced changesin Vc,in thepresence ofeither Cs or Rb were notsignificantly ://ru
p
differentfromoneanother, even though thedepolarization of Vc,caused by Cs' re
wassignificantly smallerthan that caused by Rb+(Fig. 5). Thedepolarizations of ss.org
Vc,caused by 10 mM/liter Cs' and Rb+were not significantly differentfromthe /jgp
/a
depolarizations caused by 47.5 mM/liter of these ions (Demarest and Finn, rticle
1987).Thus, 10 mM/liter ofeither ionis sufficient to produceamaximal effect. -p
Cs' and Rb+ caused significantly smaller hyperpolarizations of Vmc than Bat+. df/89
Rb+ hyperpolarized Vmc by 14.9 ± 1.7 mV and Cs' hyperpolarized Vmc by only /4/5
6
3
9.7 ± 1.3 mV, or slightly more than halfofthehyperpolarization caused by Bat+ /1
2
4
(Table I). Both ions significantly inhibited Na' transport. Cs+ reducedtheIx by 66
8
19 ±4% (P < 0.05, n = 11)and Rb+reduced theI,cby 22 ± 4% (P < 0.05, n= 3/5
6
9). However, neitherion wasas effectiveat depolarizing V,,or inhibiting theI,r 3.p
d
as 0.5 mM/liter Bat+ (Table I). Aswas thecase for Bat+, theeffects of Cs+ and f by g
Rb+ were completely reversible. ue
Tables V and VI demonstrate that both Cs'and Rb+at 10 mM/liter were as st on
effective as Bat+ in increasing Rb and Ra. Although small depolarizations of Eb 30 Ja
andhyperpolarizations ofEawere produced byCs'andRb+, none oftheeffects nu
a
wassignificant.TheeffectsofCs'andRb+areconsistentwith theabilityofthese ry 2
0
ions to reduce the transference number of thebasolateral membrane for K+ by 23
competitivelyblockingthe K+conductancepathways (DemarestandFinn, 1987).
TABLE VI
Effects ofSerosal Rb(10 mM) onCell
MembraneResistancesand ElectromotiveForces
R, Rb E, Eb
W-cm, W-cm, mV mV
Control 7.23±1.21 1.92±0.48 -92±19 -104±11
Rb 8.65±1.31 3.93±0.56 -107±15 -92±11
A 1.42±0.60 2.01±0.38 -15±9 12±8
P <0.05 <0.001 NS NS
n = 9. NS, no significant difference. Ais the differencebetween control and Rb-
treated tissues.
(cid:9)(cid:9)
572 THEJOURNAL OF GENERAL PHYSIOLOGY " VOLUME 89 " 1987
Relationship between the Na* Transport and Membrane Conductances
The relationship between the calculated I5c and the apical (G.) and basolateral
(Gb)membrane conductances for Bat+-treated bladdersare shownin Figs. 6 and
7. The lines connect points measured in the same cell before and after serosal
Ba'+. There are highly significant correlations between the I, and Ga and Gb
both before and after Bat+ exposure. The correlation coefficients for each
.
membranewere not significantly affected by Bat+
oontrol 0 r=0.864 P<0.001
D
barium o r=0.799 P<0.001 ow
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0.10 0.30 0.50 .pd
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FIGURE 6. Relationshipbetween theshort-circuit current(Ix)andtheapical mem- st o
n
braneconductance (G.) in the absence (opencircles)and presence (filled circles) of 3
0
0.5mM/literserosal Bat+. The linesconnect points for thetwo conditionsmeasured Ja
n
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isntatthee>s2a5mesacfetlelrfBorat1+6edxipfofseruernet.bladders. The Bali"valuesareforthe quasi-steady ary 20
2
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Table VII shows the slopes and y-intercepts for regression lines fitted to the
IS,, Ga, and Gb data for all ofthe bladders inhibited with Bat+, Cs', or Rb'. All
of the regressions were highly significant and none of the y-intercepts was
significantly different from zero. Blocking the basolateral K+ conductance did
not significantly alter the regression coefficients for either membrane. Thus,
blocking the K+conductance shifts Ga, Gb, and I, to lowervaluesalong the lines
that describe the bladder-to-bladder variability, without changing the slopes or
y-intercepts ofthese relationships.'
sThecalculatedIx isusedhere merely asan indexofNa+transport rate, i.e.,pumpactivity.
SincetheIxisnotincludedintheequivalentcircuit thatwehaveused,the slopesand intercepts
oftherelationships between theI, and themembraneconductances havenotbeeninterpreted
further.