In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs

274 Pages·2016·38.34 MB·English

by  Shinichi Ohno, Nobuhiko Ohno, Nobuo Terada (eds.)

In Vivo Cryotechnique in
Biomedical Research and
Application for Bioimaging
of Living Animal Organs
Shinichi Ohno
Nobuhiko Ohno
Nobuo Terada
Editors
123
I n Vivo Cryotechnique in Biomedical Research
and Application for Bioimaging of Living
Animal Organs
S hinichi O hno • N obuhiko O hno
N obuo T erada
E ditors
I n Vivo Cryotechnique in
Biomedical Research and
Application for Bioimaging
of Living Animal Organs
E ditors
S hinichi O hno N obuhiko O hno
D epartment of Anatomy and Molecular Histology D epartment of Anatomy and Molecular Histology
Interdisciplinary Graduate School of Medicine Interdisciplinary Graduate School of Medicine
and Engineering and Engineering
U niversity of Yamanashi U niversity of Yamanashi
Y amanashi, J apan Y amanashi, J apan
N obuo T erada
D ivision of Health Sciences
Shinshu University Graduate School of Medicine
Nagano, Japan
Department of Anatomy and Molecular Histology
Interdisciplinary Graduate School of Medicine
and Engineering
U niversity of Yamanashi
Y amanashi, J apan
I SBN 978-4-431-55722-7 ISBN 978-4-431-55723-4 (eBook)
D OI 10.1007/978-4-431-55723-4
L ibrary of Congress Control Number: 2015952637
S pringer Tokyo Heidelberg New York Dordrecht London
© Springer Japan 2 016
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P refa ce
W e medical and biological scientists want to know the real in vivo morphology and also the
immunolocalizations of all molecular components in functioning cells and tissues of living
animal organs; we need the “living animal morphology,” not the dead (nor conventionally
prepared with fi xatives) animal one. Recently, the live imaging of cells and tissues of animals
with fl uorescence-labeled proteins by gene manipulation has become increasingly popular in
biological fi elds. For those reasons, we should now examine the histological or immunohisto-
chemical tissue sections of living animal organs which must be exactly compatible with their
live imaging, usually clarifi ed by recently developed digital imaging techniques.
I n our opinion, the already developed immunohistochemical or morphological research per-
formed by in vivo cryotechnique (IVCT) depends exclusively on functioning cells and tissues
of living animal organs, so it is now necessary to publish a new, innovative book, reviewing
especially the real morphofunctional fi ndings in vivo of cells and tissues of living animal
organs. However, until now there has been no such book about living animal morphology on
permanent tissue section preparations corresponding to the digital live imaging of fl uorescence-
labeled soluble or structural molecules.
I n the past few decades, we have developed an original IVCT to capture the dynamically
changing morphology and immunolocalizations of signal molecules and receptors of cells and
tissues in addition to detecting soluble probes of living animal organs, reported in more than
60 biomedical papers in the last 20 years. Therefore, the publication of such an innovative
review focusing on our research fi eld is both signifi cant and timely.
We hope that this volume will provide many readers with a good opportunity to learn about
IVCT, including cryobiopsy, for their future studies. Finally, we are grateful to all the authors
who have contributed to this publication.
Y amanashi, J apan S hinichi O hno
Y amanashi, J apan N obuhiko O hno
N agano, J apan N obuo T erada
v
C ontents
P art I Overview
1 I ntroduction .............................................................................................................. 3
S hinichi O hno
2 B iomedical Significance and Development of “IVCT” ......................................... 7
S hinichi O hno
3 H ow to Perform “IVCT” ......................................................................................... 11
S hinichi O hno
4 T echnical Merits with “IVCT” ............................................................................... 15
S hinichi O hno
P art II Application of ‘IVCT’ to Various Mouse Organs
5 H istochemical Analyses of Living Mouse Liver Under Different
Hemodynamic Conditions ....................................................................................... 19
N obuhiko O hno, N obuo T erada, and S hinichi O hno
6 T hree-Dimensional Reconstruction of Liver Tissues with Confocal
Laser Scanning Microscopy .................................................................................... 25
Y urika S aitoh, N obuo T erada, N obuhiko O hno, and S hinichi O hno
7 A pplication of “In Vivo Cryotechnique” to Immunohistochemical
Detection of Hypoxia in Mouse Liver Tissues Treated with Pimonidazole ......... 29
N obuo T erada, Y urika S aitoh, N obuhiko O hno, and S hinichi O hno
8 I mmunohistochemical Detection of Soluble Immunoglobulins
in Small Intestines .................................................................................................... 33
S atoshi S himo, S ei S aitoh, Y urika S aitoh, N obuhiko O hno,
and S hinichi O hno
9 D etection of MAPK Signal Transduction Proteins
in an Ischemia/Reperfusion Model of Small Intestines ........................................ 39
J iaorong C hen, N obuo T erada, Y urika S aitoh, Z heng H uang,
N obuhiko O hno, and S hinichi O hno
1 0 H istological Study and LYVE-1 Immunolocalization of Mesenteric
Lymph Nodes ............................................................................................................ 45
Y uqin B ai, B ao W u, N obuo T erada, N obuhiko O hno, Y urika S aitoh,
and S hinichi O hno
1 1 D istribution of Immunoglobulin-P roducing Cells in Immunized
Mouse Spleens .......................................................................................................... 51
S ei S aitoh, N obuo T erada, N obuhiko O hno, and S hinichi O hno
vii
viii Contents
1 2 A lteration of Erythrocyte Shapes in Various Organs of Living
Mice or Under Blood Flow Conditions .................................................................. 57
S hinichi O hno, N obuo T erada, and Y asuhisa F ujii
1 3 D ynamic Ultrastructure of Smooth Muscle Cells in Dystrophin-Deficient
mdx or Normal scn Mice ......................................................................................... 63
S hinichi O hno
1 4 D ynamic Bioimaging of Serum Proteins in Beating Mouse Hearts..................... 67
L iye S hi, N obuo T erada, Y urika S aitoh, N obuhiko O hno,
and S hinichi O hno
1 5 H istochemical Analyses and Quantum Dot Imaging of Microvascular
Blood Flow with Pulmonary Edema ...................................................................... 77
Y urika S aitoh, N obuo T erada, N obuhiko O hno, and S hinichi O hno
1 6 D ynamic Ultrastructure of Pulmonary Alveoli of Living Mice
Under Respiratory Conditions ................................................................................ 83
S hinichi O hno, N obuo T erada, and Y asuhisa F ujii
1 7 I mmunohistochemical Analysis of Various Serum Proteins
in Living Mouse Thymus ......................................................................................... 87
Y uqin B ai, B ao W u, N obuo T erada, Y urika S aitoh, N obuhiko O hno,
S ei S aitoh, and S hinichi O hno
1 8 I mmunohistochemistry and Enzyme Histochemistry of HRP for
Demonstration of Blood Vessel Permeability in Mouse Thymic Tissues ............. 93
B ao W u, N obuhiko O hno, Y urika S aitoh, Y uqin B ai, Z heng H uang,
N obuo T erada, and S hinichi O hno
1 9 I mmunolocalization of Serum Proteins in Living Mouse
Glomeruli Under Various Hemodynamic Conditions........................................... 101
Z ilong L i, N obuhiko O hno, N obuo T erada, and S hinichi O hno
2 0 I mmunohistochemical Analyses on Albumin and IgG in Acute
Hypertensive Mouse Kidneys .................................................................................. 105
Z ilong L i, N obuo T erada, N obuhiko O hno, and S hinichi O hno
2 1 A pplication of Novel “In Vivo Cryotechnique” in Living
Animal Kidneys ........................................................................................................ 109
Z ilong L i, X iaoyue Z hai, N obuhiko O hno, N obuo T erada,
and S hinichi O hno
2 2 A pplication of Periodic Acid-Schiff Fluorescence Emission
for Immunohistochemistry of Living Mouse Renal Glomeruli ........................... 115
Z ilong L i, N obuo T erada, and S hinichi O hno
2 3 I mmunohistochemical Analyses on Serum Proteins
in Nephrons of Protein-Overload Mice .................................................................. 121
N obuhiko O hno, D aoyuan Z hou, N obuo T erada, and S hinichi O hno
2 4 I mmunohistochemical Analyses of Serum Proteins in Puromycin
Aminonucleoside Nephropathy of Living Rat Kidneys ........................................ 127
E ri K awashima, Y urika S aitoh, N obuo T erada, N obuhiko O hno,
K iyoko I nui, A shio Y oshimura, and S hinichi O hno
2 5 I mmunolocalization of Phospho-Arg-D irected Protein Kinase-Substrate
in Hypoxic Kidneys .................................................................................................. 135
S ei S aitoh, N obuo T erada, N obuhiko O hno, Y urika S aitoh,
a nd S hinichi O hno
Contents ix
2 6 D ynamic Ultrastructures of Renal Glomeruli in Living Mice
Under Various Hemodynamic Conditions ............................................................. 141
S hinichi O hno, Y asuhisa F ujii, and N obuo T erada
2 7 F unction of Dynamin-2 in the Formation of Discoid Vesicles
in Urinary Bladder Umbrella Cells ........................................................................ 145
N obuo T erada, Y urika S aitoh, N obuhiko O hno, Y asuhisa F ujii,
and S hinichi O hno
2 8 I nvolvement of Follicular Basement Membrane and Vascular
Endothelium in Blood-Follicle Barrier Formation of Mice.................................. 149
N obuhiko O hno, H ong Z hou, N obuo T erada, and S hinichi O hno
2 9 P ermselectivity of Blood-Follicle Barriers in Mouse
Polycystic Ovary Model ........................................................................................... 155
N obuhiko O hno, H ong Z hou, N obuo T erada, and S hinichi O hno
3 0 A pplication of “In Vivo Cryotechnique” to Immunohistochemical
Study of Serum Albumin in Normal and Cadmium-Treated
Mouse Testis Organs ................................................................................................ 161
N obuo T erada, Y urika S aitoh, N obuhiko O hno, X iaogang L iao,
and S hinichi O hno
3 1 I mmunohistochemical Detection of Angiotensin II Receptors
in Mouse Cerebellum ............................................................................................... 165
Z heng H uang, N obuhiko O hno, N obuo T erada, Y urika S aitoh,
J iaorong C hen, and S hinichi O hno
3 2 A pplication of “In Vivo Cryotechnique” to Immunohistochemical
Analyses for Effects of Anoxia on Serum Immunoglobulin and Albumin
Leakage Through Blood–Brain Barrier in Mouse Cerebellum ........................... 171
N obuo T erada, Y urika S aitoh, N obuhiko O hno, Z agreb Z ea-Aragón,
and S hinichi O hno
3 3 E xtracellular Space in Central Nervous System ................................................... 175
N obuhiko O hno, N obuo T erada, and S hinichi O hno
3 4 O verview on Recent Applications of In Vivo Cryotechnique
in Neurosciences ....................................................................................................... 179
N obuhiko O hno, N obuo T erada, Y urika S aitoh, and S hinichi O hno
3 5 A pplication of “In Vivo Cryotechnique” to Immunohistochemical
Detection of Phosphorylated Rhodopsin in Light- Exposed Retina
of Living Mouse ........................................................................................................ 185
N obuo T erada, Y urika S aitoh, N obuhiko O hno, and S hinichi O hno
3 6 A pplication of “In Vivo Cryotechnique” to Immunohistochemical
Detection of Glutamate in Mouse Retina Inner Segment
of Photoreceptors ..................................................................................................... 189
N obuo T erada, Y urika S aitoh, N obuhiko O hno, and S hinichi O hno
3 7 A pplication of “In Vivo Cryotechnique” to Immunohistochemical
Study of Mouse Sciatic Nerves Under Various Stretching Conditions ................ 193
A kio K amijo, Y urika S aitoh, N obuhiko O hno, S hinichi O hno,
and N obuo T erada